• 제목/요약/키워드: Dead Water

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고분자 전해질 연료전지를 이용한 Laptop Computer용 전원 공급 장치 (Fuel Cell for the Energy Supply of Laptop Computers)

  • 권부길;이현준;황정태
    • 한국전기화학회:학술대회논문집
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    • 한국전기화학회 2003년도 연료전지심포지움 2003논문집
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    • pp.151-154
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    • 2003
  • The lap-top with a integrated fuel cell system presented here proves that these solutions have a great potential. Though, there are things that have to be improved before they are introduced to the marked. In this system, heat and water management as well as dead end operation are critical issues which must be directly addressed in further research. Both modified stack design and other new cooling techniques are possible ways to continue the work.

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Rapid Detection of Viable Escherichia coli O157 by Coupling Propidium Monoazide with Loop-Mediated Isothermal Amplification

  • Zhao, Xihong;Wang, Jun;Forghani, Fereidoun;Park, Joong-Hyun;Park, Myoung-Su;Seo, Kun-Ho;Oh, Deog-Hwan
    • Journal of Microbiology and Biotechnology
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    • 제23권12호
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    • pp.1708-1716
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    • 2013
  • Conventional molecular detection methods cannot distinguish between viable and dead Escherichia coli O157 cells. In this study, the loop-mediated isothermal amplification (LAMP) method combined with propidium monoazide (PMA) treatment was developed to selectively detect viable E. coli O157 cells. Four primers, including outer primers and inner primers, were specially designed for the recognition of six distinct sequences on the serogroups (O157) of the specific rfbE gene of the E. coli O157 genome. PMA selectively penetrated through the compromised cell membranes and intercalated into DNA. Amplification of DNA from dead cells was completely inhibited by $3.0{\mu}g/ml$ PMA, whereas the DNA derived from viable cells was amplified remarkably within 1 h by PMA-LAMP. Exhibiting high sensitivity and specificity, PMA-LAMP is a suitable method for evaluating the inactivation efficacy of slightly acidic electrolyzed water in broth. PMA-LAMP can selectively detect viable E. coli O157 cells. This study offers a novel molecular detection method to distinguish between viable and dead E. coli O157 cells.

오픈 캐소드형 고분자전해질 연료전지의 성능향상과 회복기법 (Performance Enhancement and Recovery Method of Open Cathode PEMFC)

  • 이기택;김준범
    • 공업화학
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    • 제28권1호
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    • pp.118-124
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    • 2017
  • 공랭식 고분자전해질 연료전지는 개방된 cathode구조로 인하여 시스템의 단순화와 부품 수 저감의 장점이 있다. 공랭식 연료전지는 최근에 많이 연구되고 있지만, 성능이 외부 환경에 영향을 받으며, 공기의 상대습도가 낮은 경우 전해질막의 건조로 인한 성능 감소가 발생할 수 있다. 본 연구에서는 공랭식 연료전지의 성능에 영향을 주는 요인인 cathode 측 공기 유량과 anode 측 purge interval영향에 대해 분석하였으며, 스택을 운전하지 않는 상태로 장기간 보관하는 것이 성능에 미치는 영향에 대하여 실험을 수행하였다. 연료전지 외부에 설치한 fan의 전압을 조절하면 cathode 측 공기의 공급유량을 변화시킬 수 있고 스택의 온도도 제어할 수 있으므로, fan전압은 공랭식 연료전지의 성능에 영향을 주는 중요한 인자이다. 연료전지 시스템을 단순화하고 수소의 사용률을 높이기 위하여 anode 측은 dead ended anode (DEA) 기법을 사용하였다. 주기적인 purge를 실행하여 생성된 물과 가스를 배출하였으며, purge 주기를 변경하면서 스택의 성능에 미치는 영향에 대하여 실험을 수행하였다. 스택의 보관기간이 길어질수록 membrane dehydration으로 인해 성능이 감소하는 것을 실험을 통해 파악하였고, 단시간에 성능을 회복할 수 있는 기법을 제시하였다.

시동/정지 반복에 의한 데드엔드형 고분자전해질 연료전지의 성능 감소 (Performance Degradation of Dead-end Type PEMFC by Startup and Shutdown Cycles)

  • 정재현;정재진;송명현;정회범;나일채;이호;박권필
    • Korean Chemical Engineering Research
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    • 제51권5호
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    • pp.540-544
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    • 2013
  • 고분자전해질연료전지(PEMFC)는 시동/정지과정에서 성능과 수명이 감소한다. 본 연구에서는 캐소드가스로 산소를 사용하는 데드엔드 형 PEMFC의 시동/정지 과정의 영향을 분극곡선, 임피던스(EIS), SEM과 TEM을 사용해 연구하였다. 시동/정지 과정에서 PEMFC 성능감소를 막기 위해서는 더미 로드를 사용해야 함을 보였다. 시동/정지 반복과정 중 50% 상대습도(RH)에서 캐소드 카본지지체의 부식에 의한 열화가 100% RH보다 심했다. 데드엔드 형 PEMFC의 정지과정에서 PEMFC에 물을 공급해줌으로써 50% RH에서 열화속도를 감소시켰다.

국내 사용 농약을 대상으로 한 HPLC 방법에 의한 옥탄올/물 분배계수 추정법의 적용성 검토 (Applicability of the HPLC Method for the Estimation of Octanol/water Partition Coefficient to Pesticides of Domestic Use)

  • 김균;권진욱;김용화
    • Environmental Analysis Health and Toxicology
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    • 제16권4호
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    • pp.189-196
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    • 2001
  • Octanol/water partition coefficients of 52 chemicals were calculated using RP-HPLC estimation method and predicted by computer program, PCHEM. The result showed relationship between literature values and RP-HPLC observed values (relative coefficient r$^2$=0.916), but the relationship of PCHEM values with literature values was lower than RP-HPLC value (relative coefficient r$^2$=0.795). The average difference in partition coefficient between the RP-HPLC method and flask-shaking method was log Kow=0.54, while the average difference between the values predicted form the computer program and flask- shaking method was log Kow = 0.36 Compared to octanol/water partition coefficients by 3 methods (Flask-shaking, RP-HPLC, computer prediction), the octanol/water partition coefficient values based on the flask-shaking method were very similar to the literature values, while the octanol/water partition coefficient values by RP-HPLC method without to consider the dead time, and computer prediction values did not significantly differ with the literature values.

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A new method for in line electrokinetic characterization of cakes

  • Lanteri, Yannick;Ballout, Wael;Fievet, Patrick;Deon, Sebastien;Szymczyk, Anthony;Sauvade, Patrick
    • Membrane and Water Treatment
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    • 제4권3호
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    • pp.157-174
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    • 2013
  • The present study is devoted to the validation of a new method for in line electrokinetic characterisation of deposits on membrane surfaces. This method is based upon simultaneous measurements of transversal streaming potential and permeates flux at constant pressure before and during the deposit formation. Dead-end filtration experiments were conducted with negative flat membranes forming a narrow slit channel, negative hollow fiber membranes and mono-dispersed suspensions of (negatively charged) polystyrene latex and (positively charged) melamine particles at various concentrations. It was observed that the overall streaming potential coefficient increased in absolute value with the deposited latex quantity, whereas it decreased and changed of sign during the filtration of melamine suspensions. By considering a resistance-in-series model, the streaming potential coefficient of the single deposit ($SP_d$) was deduced from the electrokinetic and hydraulic measurements. The independence of $SP_d$ with respect to growth kinetics validates the measurement method and the reliability of the proposed procedure for calculating $SP_d$. It was found that $SP_d$ levelled off much more quickly when filtration was performed through the slit channel. This different behaviour could result from a non-uniform distribution of the deposit thickness along the membrane given that the position of measuring electrodes is different between the two cells.

인산염 마그네시아 시멘트에 의한 중금속 이온 고정화 (Solidification of Heavy Metal Ions Using Magnesia-phosphate Cement)

  • 최훈;강현주;송명신;정의담;김주성
    • 한국세라믹학회지
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    • 제48권1호
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    • pp.20-25
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    • 2011
  • Since 1980's, many mines have been closed and abandoned due to the exhaustion of deposits and declining prices of international mineral resources. Because of the lack of post management for these abandoned mines, Farm land and rivers were contaminated with heavy metal ions and sludge. We studied on the solidification/stabilization of heavy metal ions, chromium ions and lead ions, using magnesia-phosphate cement. Magnesia binders were used calcined-magnesia and dead-burned magnesia. Test specimens were prepared by mixing magnesia binder with chromium ions and lead ions and activators. We analyzed the hydrates by reaction between magnesiaphosphate cement and each heavy metal ions by XRD and SEM-EDAX, and analyzed the content of heavy metal ions in the eruption water from the specimens for the solidification and stabilization of heavy metal ions by ICP. The results was shown that calcined magnesia binder is effective in stabilization for chromium ions and dead-burned magnesia binder is effective in stabilization for lead ions.

브레이크 스위치 결함에 의한 간헐적인 가속불량 현상의 고장진단 사례연구 (Case Study of Intermittent Poor Acceleration Fault Diagnosis by Brake Switch Fault)

  • 김성모;조행득
    • 한국생산제조학회지
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    • 제24권2호
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    • pp.203-210
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    • 2015
  • This paper investigates the failure of a car with a 2.5-liter CRDi engine of the Hyundai Company. The failure is caused by intermittent poor acceleration while driving. To analyze the cause, we investigated the air intake volume, the fuel injection, and the air-fuel ratio, which were determined to be normal. The brake switch signal error was discovered while analyzing the function that limits the output of the engine. While investigating the cause, we discovered the corrosion of the pins on the connector of the brake switch. We determined that it was generated by soapy water flowing in the solar film. Therefore, the cause of the failure was the brake switch signal errors. Additionally, we determined that ECM was the normal fail-safe mode that implemented the override device for safety during normal acceleration. Based on these results, further solar film experiments must be conducted to fully elucidate the causes.

퇴화처리에 따른 참깨, 파, 상추 종자의 당, 아미노산, 단백질 누출 (Leakage of Sugars, Amino Acids and Protein from Differently - Aged Seeds of Sesame, Welsh Onion and Lettuce)

  • 이석순;홍승범
    • 한국작물학회지
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    • 제40권4호
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    • pp.407-412
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    • 1995
  • 비파괴적인 방법으로 활력이 높은 종자를 선별하는 방법을 개발하기 위하여, 참깨, 파, 상추 종자를 상대습도 90%, 온도 45$^{\circ}C$에서 인위적으로 퇴화시켜 활력이 다른 종자를 물에 침지하였을 때 종자가 분비하는 당, 아미노산, 단백질의 양과 속도 등을 조사한 결과를 요약하면 다음과 같다. 1. 참깨 종자는 활력과 침지시간에 관계없이 당, 아미노산, 단백질을 거의 분비하지 않았다. 2. 파의 죽은 종자는 건전종자나 활력이 중인 종자보다 아미노산을 더 많이 분비하였으나 전당과 단백질 분비량은 크지 않았다. 3. 상추의 죽은 종자는 당, 아미노산, 단백질을 모두 많이 분비하였다. 건전종자와 활력이 중인 종자는 당은 거의 분비하지 않았으나 아미노산과 단백질은 다소 많이 분비하였다.

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