• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.15 no.1
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• pp.31-36
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• 2004

Background and Objectives : It has been shown that the epilaryngeal tube in the human airway is responsible for vocal ring, or the singer's formant. In previous study, authors showed that in trained tenors, besides the conventional singer's formant in the region of ,5500Hz, another energy peak was observed in the region of 8,000Hz. This peak was interpreted as the second resonance of the epilarynx tube. Singers in other voice categories who produce vocal ring are assumed to have the same peak, but no measurements have as yet been made. Materials and Methods : Fifteen tenors, fourteen baritones, seven sopranos and five mezzo sopranos attending the music college, department of vocal music who could reliably produce the head and chest registers were chosen for this study. Each subject was asked to produce an/ah/sound for at least three seconds for the head register sound(tenors ; G4, barions ; E4 sopranos ; F5 and mezzosopranos ; C5) and for the chest register sound (tenors ; C3, baritones ; D3, sopranos ; D4 and Mezzosoprano ; A3). The sound data was analyzed using the Fast Fourier Transform (FFT)-based power spectrum, Long term average(LTA) power spectrum using the FFT algorithm of the Computerized Speech Lab (CSL, Kay elemetrics, Model 4300B, USA). Statistical analysis was performed using the Mann-Whitney test of the Statistical Package for Social sciences(SPSS). Results : For head register sounds, a significant increase was seen in the 2,200-3,400Hz region(p<0.05) and the Similar to the head register sounds, there was a significant increase in energy in the four trained singer group compared with the untrained group in the 2,200-3,100Hz region(p<0.05), the 7,800-8,400Hz region(p<0.05) for the chest register sounds. Conclusions : When good vocal production was made for the head and chest registers, an energy peak was observed near 2,500Hz, a frequency already known as the "singer's formant', in all subjects in the study group. Another region of increased energy was observed around 8,000Hz that had not been noticed previously. The authors believe this region to be the second singer's formant.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.11
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• pp.1530-1540
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• 2016

This study was conducted to estimate the genetic parameters of ${\beta}$-hydroxybutyrate (BHBA) and acetone concentration in milk by Fourier transform infrared spectroscopy along with test-day milk production traits including fat %, protein % and milk yield based on monthly samples of milk obtained as part of a routine milk recording program in Korea. Additionally, the feasibility of using such data in the official dairy cattle breeding system for selection of cows with low susceptibility of ketosis was evaluated. A total of 57,190 monthly test-day records for parities 1, 2, and 3 of 7,895 cows with pedigree information were collected from April 2012 to August 2014 from herds enrolled in the Korea Animal Improvement Association. Multi-trait random regression models were separately applied to estimate genetic parameters of test-day records for each parity. The model included fixed herd test-day effects, calving age and season effects, and random regressions for additive genetic and permanent environmental effects. Abundance of variation of acetone may provide a more sensitive indication of ketosis than many zero observations in concentration of milk BHBA. Heritabilities of milk BHBA levels ranged from 0.04 to 0.17 with a mean of 0.09 for the interval between 4 and 305 days in milk during three lactations. The average heritabilities for milk acetone concentration were 0.29, 0.29, and 0.22 for parities 1, 2, and 3, respectively. There was no clear genetic association of the concentration of two ketone bodies with three test-day milk production traits, even if some correlations among breeding values of the test-day records in this study were observed. These results suggest that genetic selection for low susceptibility of ketosis in early lactation is possible. Further, it is desirable for the breeding scheme of dairy cattle to include the records of milk acetone rather than the records of milk BHBA.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.5
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• pp.2743-2746
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• 2013

Background: Dilantin sodium (phenytoin) is an antiepileptic drug, which is routinely used to control generalized tonic clonic seizure and partial seizure episodes. A few case reports of oral squamous cell carcinomas arising from regions of phenytoin induced gingival overgrowth (GO), and overexpression of mitogenic factors and p53 have presented this condition as a pathology with potential to transform into malignancy. We recently investigated the genetic status of p53 and H-ras, which are known to be frequently mutated in Indian oral carcinomas in GO tissues and found them to only contain wild type sequences, which suggested a non-neoplastic nature of phenytoin induced GO. However, besides p53 and H-ras, other oncogenes and tumor suppressors such as PIK3CA, p14ARF, p16INK4a and $p21^{Waf1/Cip1}$, are frequently altered in oral squamous cell carcinoma, and hence are required to be analyzed in phenytoin induced GO tissues to be affirmative of its non-neoplastic nature. Methods: 100ng of chromosomal DNA isolated from twenty gingival overgrowth tissues were amplified with primers for exons 9 and 20 of PIK3CA, exons $1{\alpha}$, $1{\beta}$ and 2 of p16INK4a and p14ARF, and exon 2 of $p21^{Waf1/Cip1}$, in independent reactions. PCR amplicons were subsequently gel purified and eluted products were sequenced. Results: Sequencing analysis of the twenty samples of phenytoin induced gingival growth showed no mutations in the analyzed exons of PIK3CA, p14ARF, p16INK4a and $p21^{Waf1/Cip1}$. Conclusion: The present data indicate that the mutational alterations of genes, PIK3CA, p14ARF, p16INK4a and $p21^{Waf1/Cip1}$ that are frequently mutated in oral squamous cell carcinomas are rare in phenytoin induced gingival growth. Thus the findings provide further evidence that phenytoin induced gingival overgrowth as a non-neoplastic lesion, which may be considered as clinically significant given the fact that the epileptic patients are routinely administered with phenytoin for the rest of their lives to control seizure episodes.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.49 no.3
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• pp.301-309
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• 2016

To facilitate the effective use of butter clam shell as a natural calcium resource, we determined the optimal conditions for calcium lactate (BCCL) preparation with high solubility using response surface methodology (RSM). The polynomial models developed by RSM for pH, solubility and yield were highly effective in describing the relationships between factors (P<0.05). Increased molar ratios of calcined powder (BCCP) from butter clam shell led to reduced solubility, yield, color values and overall quality. The critical values of multiple response optimization to independent variables were 1.75 M and 0.94 M for lactic acid and BCCP, respectively. The actual values (pH 7.23, 97.42% for solubility and 423.22% for yield) under optimization conditions were similar to the predicted values. White indices of BCCLs were in the range of 86.70–90.86. Therefore, organic acid treatment improved color value. The buffering capacity of BCCLs was strong, at pH 2.82 to 3.80, upon the addition of less than 2 mL of 1 N HCl. The calcium content and solubility of BCCLs were 6.2–16.7 g/100 g and 93.6-98.5%, respectively. Fourier transform analysis of infrared spectroscopy data identified BCCL as calcium lactate pentahydrate, and the analysis of microstructure by field emission scanning electron microscopy revealed an irregular form.

• Journal of Korea Water Resources Association
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• v.41 no.9
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• pp.959-967
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• 2008

Disaggregation techniques are widely used to transform observed daily rainfall values into hourly ones, which serve as important inputs for flood forecasting purposes. However, an important limitation with most of the existing disaggregation techniques is that they treat the rainfall process as a realization of a stochastic process, thus raising questions on the lack of connection between the structure of the models on one hand and the underlying physics of the rainfall process on the other. The present study introduces a nonlinear deterministic (and specifically chaotic) framework to study the dynamic characteristics of rainfall distributions across different temporal scales (i.e. weights between scales), and thus the possibility of rainfall disaggregation. Rainfall data from the Seoul station (recorded by the Korea Meteorological Administration) are considered for the present investigation, and weights between only successively doubled resolutions (i.e., 24-hr to 12-hr, 12-hr to 6-hr, 6-hr to 3-hr) are analyzed. The correlation dimension method is employed to investigate the presence of chaotic behavior in the time series of weights, and a local approximation technique is employed for rainfall disaggregation. The results indicate the presence of chaotic behavior in the dynamics of weights between the successively doubled scales studied. The modeled (disaggregated) rainfall values are found to be in good agreement with the observed ones in their overall matching (e.g. correlation coefficient and low mean square error). While the general trend (rainfall amount and time of occurrence) is clearly captured, an underestimation of the maximum values are found.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.12
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• pp.452-458
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• 2017

In this study, a device was developed for diagnosing depression using EEG signals from July 2016 to June 2017. For normal people, the left alpha rhythm is more activated than the right alpha rhythm, but for the depressed patients, the right alpha rhythm is more activated than the left one. An analog circuit and digital low pass filter were used for noise removal and amplification of EEG, and the Hamming window function was applied to eliminate the signal leakage generated by the fast Fourier transform. To verify the validity of the developed diagnosis system, the EEG of 20 university students in the 3rd and 4th grade with an average age of 24 years was measured. Calculations of the relative value of the left and right alpha rhythm for the depression diagnosis revealed a minimum, maximum, and mean value of 66.7, 113.3, and 92.2, respectively. In addition, 7 out of 20 subjects were between 90 and 95, and those with a higher mean deviation of approximately 20 tended to have mild depression. These results can provide meaningful data for the development of depression treatment equipment by solving the left and right brain asymmetry problem, and it may be applied usefully to diagnose depression after clinical trials on a large number of depressed patients.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.9 no.1
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• pp.99-111
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• 1999

The purpose of this study was to evaluate crystalline silica contents in airborne respirable dusts from various manufacturing industries and to compare analytical ability of two different methods of quantifying crystalline silica, X-ray diffraction(XRD) and Fourie transform infrared spectroscopy(FTIR). Various manufacturing industries with a history of having pneumoconiosis cases and also known to generate dusts containing crystalline silica were investigated. These industries include: ceramics, brick, concrete, and abrasive material etc. The personal respirable dust samples were collected using l0mm, Dorr-Oliver nylon cyclone equipped with 37mm, $5{\mu}m$ pore size. polyvinylchloride (PVC) filters as collection media. All samples were weighed before and after sampling and were pretreated according to the NIOSH sampling and analytical methods 7500, and 7602 for dust collection and quartz analysis. A total of 48 samples were collected from these industries. Initial analyses of these samples showed log-normal distributions for dust and quartz concentrations. Some results from ceramics and stone exceeded current Korean Occupational Exposure Limits. The average concentrations of personal respirable dust by cyclone were 0.43, 0.24, 0.26, 0.42, 0.53 and $0.29mg/m^3$ in ceramics, stone, concrete, glass, briquets, and others, respectively. A comparison of performance of two analytical methods for quantifying crystalline silica was performed using data from ceramics. The results showed that no significant difference was found between two methods for ceramics. The mean crystalline silica contents determined by XRD were 3.41 % of samples from briquets and 7.18 % from ceramics and were 2.58 % from concrete and 10.33 % from ceramics by FTIR. For crystalline silica analysis, two analytical techniques were highly correlated with $r^2=0.81$ from ceramics. Both cristobalite and tridymite were not detected by XRD and FTIR.

• Horticultural Science & Technology
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• v.31 no.6
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• pp.748-755
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• 2013

This study was conducted to find a salt tolerance gene in Brassica rapa. In order to meet this objective, we analyzed data from a KBGP-24K oligo chip [BrEMD (Brassica rapa EST and microarray database)] of the B. rapa ssp. pekinensis 'Chiifu' under salt stress (250 mM NaCl). From the B. rapa KBGP-24K microarray chip analysis, 202 salt-responsive unigenes were primarily selected under salt stress. Of these, a gene with unknown function but known full-length sequence was chosen to closely investigate the gene function. The selected gene was named BrSSR (B. rapa salt stress resistance). BrSSR contains a 285 bp open reading frame encoding a putative 94-amino acid protein, and a DUF581 domain. The pSL94 vector was designed to over-express BrSSR, and was used to transform tobacco plants for salt tolerance analysis. T1 transgenic tobacco plants that over-expressed BrSSR were selected by PCR and DNA blot analyses. Quantitative real-time RT PCR revealed that the expression of BrSSR in transgenic tobacco plants increased by approximately 3.8-fold. Similar results were obtained by RNA blot analysis. Phenotypic characteristics analysis showed that transgenic tobacco plants with over-expressed BrSSR were more salt-tolerant than the wild type control under 250 mM NaCl for 5 days. Based on these results, we hypothesized that the over-expression of BrSSR may be closely related to the enhancement of salt tolerance.

• Journal of the Society of Naval Architects of Korea
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• v.29 no.4
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• pp.114-131
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• 1992

In the present work, a two-dimensional boundary-value problem for a large amplitude motion is treated as an initial-value problem by satisfying the exact body-boundary and nonlinear free-surface boundary conditions. The present nonlinear numerical scheme is similar to that described by Vinje and Brevig(1981) who utilized the Cauchy's theorem and assumed the periodicity in the horizontal coordinate. In the present thesis, however, the periodicity in the horizontal coordinate is not assumed. Thus the present method can treat more realistic problems, which allow radiating waves to infinities. In the present method of solution, the original infinite fluid domain, is divided into two subdomains ; ie the inner and outer subdomains which are a local nonlinear subdomain and the truncated infinite linear subdomain, respectively. By imposing an appropriate matching condition, the computation is carried out only in the inner domain which includes the body. Here we adopt the nonlinear scheme of Vinje & Brevig only in the inner domain and respresent the solution in the truncated infinite subdomains by distributing the time-dependent Green function on the matching boundaries. The matching condition is that the velocity potential and stream function are required to be continuous across the matching boundary. In the computations we used, if necessary, a regriding algorithm on the free surface which could give converged stable solutions successfully even for the breaking waves. In harmonic oscillation problem, each harmonic component and time-mean force are obtained by the Fourier transform of the computed forces in the time domain. The numerical calculations are made for the following problems. $\cdot$ Forced harmonic large-amplitude oscillation(${\omega}{\neq}0,\;U=0$) $\cdot$ Translation with a uniform speed(${\omega}=0,\;U{\neq}0$) The computed results are compared with available experimental data and other analytical results.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.02a
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• pp.407-408
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• 2011

Natural boron consists of two stable isotopes 10B and 11B with natural abundance of 18.8 atom percent of 10B and 81.2 atom percent of 11B. The thermal neutron absorption cross-section for 10B and 11B are 3837 barn and 0.005 barn respectively. 10B enriched specific compounds are used for control rods and as a reactor coolant additives. In this work 2 methods for boron enrichment were analysed: 1) Gas irradiation in static conditions. Dissociation occurs due to multiphoton absorption by specific isotopes in appropriately tuned laser field. IR shifted laser pulses are usually used in combination with increasing the laser intensity also improves selectivity up to some degree. In order to prevent recombination of dissociated molecules BCl3 is mixed with H2S 2) SILARC method. Advantages of this method: a) Gas cooling is helpful to split and shrink boron isotopes absorption bands. In order to achieve better selectivity BCl3 gas has to be substantially rarefied (~0.01%-5%) in mixture with carrier gas. b) Laser intensity is lower than in the first method. Some preliminary calculations of dissociation and recombination with carrier gas molecules energetics for both methods will be demonstrated Boron separation in SILARC method can be represented as multistage process: 1) Mixture of BCl3 with carrier gas is putted in reservoir 2) Gas overcooling due to expansion through Laval nozzle 3) IR multiphoton absorption by gas irradiated by specifically tuned laser field with subsequent gradual gas condensation in outlet chamber It is planned to develop software which includes these stages. This software will rely on the following available software based on quantum molecular dynamics in external quantized field: 1) WavePacket: Each particle is treated semiclassicaly based on Wigner transform method 2) Turbomole: It is based on local density methods like density of functional methods (DFT) and its improvement- coupled clusters approach (CC) to take into account quantum correlation. These models will be used to extract information concerning kinetic coefficients, and their dependence on applied external field. Information on radiative corrections to equation of state induced by laser field which take into account possible phase transition (or crossover?) can be also revealed. This mixed phase equation of state with quantum corrections will be further used in hydrodynamical simulations. Moreover results of these hydrodynamical simulations can be compared with results of CFD calculations. The first reasonable question to ask before starting the CFD simulations is whether turbulent effects are significant or not, and how to model turbulence? The questions of laser beam parameters and outlet chamber geometry which are most optimal to make all gas volume irradiated is also discussed. Relationship between enrichment factor and stagnation pressure and temperature based on experimental data is also reported.