• Title/Summary/Keyword: Data envelope analysis

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Global Regulation of Gene Expression in the Human Gastric Pathogen Helicobacter pylori in Response to Aerobic Oxygen Tension Under a High Carbon Dioxide Level

  • Park, Shin Ae;Lee, Na Gyong
    • Journal of Microbiology and Biotechnology
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    • v.23 no.4
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    • pp.451-458
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    • 2013
  • The human gastric pathogen Helicobacter pylori (Hp) has been considered a microaerophile. However, we recently reported that, when supplied with 10% $CO_2$, Hp growth is stimulated by an atmospheric level of $O_2$, suggesting that Hp is a capnophilic aerobe. In this study, we investigated the effects of aerobic $O_2$ tension on Hp cells by comparing gene expression profiles of cultures grown under microaerobic and aerobic conditions in the presence of 10% $CO_2$. The results showed that overall differences in gene expression in Hp cells grown under the two $O_2$ conditions were predominantly growth-phase-dependent. At 6 h, numerous genes were down-regulated under the aerobic condition, accounting for our previous observation that Hp growth was retarded under this condition. At 36 h, however, diverse groups of genes involved in energy metabolism, cellular processes, transport, and cell envelope synthesis were highly up- or down-regulated under the aerobic condition, indicating a progression of the cultures from the log phase to the stationary phase. The expression of several oxidative stress-associated genes including tagD, katA, and rocF was induced in response to aerobic $O_2$ level, whereas trxA, trxB, and ahpC remained unchanged. Altogether, these data demonstrate that aerobic $O_2$ tension is not detrimental to Hp cells but stimulates Hp growth, supporting our previous finding that Hp may be an aerobic bacterium that requires a high $CO_2$ level for its growth.

Generation of Design Time History Complying With Japanese Seismic Design Standards for Nuclear Power Plants (일본 원전 내진설계 기술기준을 적용한 모의지진파(가속 도시간이력) 작성)

  • Gin, Seungmin;Kim, Yongbog;Lee, Yongsun;Moon, Il Hwan
    • Journal of the Earthquake Engineering Society of Korea
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    • v.25 no.2
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    • pp.83-91
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    • 2021
  • Seismic designs for Korean nuclear power plants (NPPs) under earthquakes' design basis are noticed due to the recent earthquake events in Korea and Japan. Japan has developed the technologies and experiences of the NPPs through theoretical research and experimental verification with extensively accumulated measurement data. This paper describes the main features of the design-time history complying with the Japanese seismic design standard. Proper seed motions in the earthquake catalog are used to generate one set of design time histories. A magnitude and epicentral distance specify the amplitude envelope function configuring the shape of the earthquake. Cumulative velocity response spectral values of the design time histories are compared and checked to the target response spectra. Spectral accelerations of the time histories and the multiple-damping target response spectra are also checked to exceed. The generated design time histories are input to the reactor building seismic analyses with fixed-base boundary conditions to calculate the seismic responses. Another set of design time histories is generated to comply with Korean seismic design procedures for NPPs and used for seismic input motions to the same reactor containment building seismic analyses. The responses at the dome apex of the building are compared and analyzed. The generated design time histories will be also applied to subsequent seismic analyses of other Korean standard NPP structures.

Structural glass panels: An integrated system

  • Bidini, G.;Barelli, L.;Buratti, C.;Castori, G.;Belloni, E.;Merli, F.;Speranzini, E.
    • Smart Structures and Systems
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    • v.30 no.3
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    • pp.327-332
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    • 2022
  • In building envelope, transparent components play an important role. The structural glazing systems are the weak element of the casing in terms of mechanical resistance, thermal and acoustic insulation. In the present work, new structural glass panels with granular aerogel in interspace were investigated from different points of view. In particular, the mechanical characterization was carried out in order to assess the resistance to bending of the single glazing pane. To this end, a special instrument system was built to define an alternative configuration of the coaxial double ring test, able to predict the fracture strength of glass large samples (400 × 400 mm) without overpressure. The thermal and lighting performance of an innovative double-glazing façade with granular aerogel was evaluated. An experimental campaign at pilot scale was developed: it is composed of two boxes of about 1.60 × 2 m2 and 2 m high together with an external weather station. The rooms, identical in terms of size, construction materials, and orientation, are equipped with a two-wing window in the south wall surface: the first one has a standard glazing solution (double glazing with air in interspace), the second room is equipped with the innovative double-glazing system with aerogel. The indoor mean air temperature and the surface temperature of the glass panes were monitored together with the illuminance data for the lighting characterization. Finally, a brief energy characterization of the performance of the material was carried out by means of dynamic simulation models when the proposed solution is applied to real case studies.

Energy Performance and Operating Cost Assessment for Implementing Green Remodeling Technologies in a Detached House (단독주택 건물 그린리모델링에 따른 건물 에너지 성능과 운전비용 절감 효과 평가)

  • Byonghu Sohn;Su-In Lee;Jae-Sik Kang
    • Journal of the Korean Society for Geothermal and Hydrothermal Energy
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    • v.19 no.4
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    • pp.27-38
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    • 2023
  • The Government the Republic of Korea is showing a lot of interest in net zero-energy buildings (NZEBs) to reduce energy consumption of buildings and to promote green growth policy in construction sector. The application of building passive technologies and renewable energies is essential to achieving NZEBs. Green remodeling reinforced the insulation of the exterior walls and roofs of the buildings and replaced high-efficiency windows and doors. In this study, the energy performance before and after green remodeling applied in a detached house was comparatively analyzed for baseline scenario and three different ones, ALT 1, ALT 2 and ALT 3. A building modeling and simulation software (DesignBuilder V7.0) with EnergyPlus (V9.4) calculation engine was used to calculate the energy demand and energy consumption for each scenario. Based on the calculation results of the building's energy demand for baseline, it was determined that the target building required more heating energy than cooling energy. The simulation results also showed that the implementation of building envelope performance improvement technologies (ALT 1) could notably decrease the heating energy consumption of the building. After the remodeling (ALT 1), the source energy consumption per unit floor area was assessed to be reduced by 65.2%, compared to prior remodeling of 338.7 kWh/m2 -y. Meanwhile, ALT 2 can achieve energy savings of 67.7% and ALT 3 can achieve savings of 73.1%. Following completion of the remodeling project, actual construction costs, and on-site measurements and verification results will be gathered and compared with the simulation results. Additionally, economic analysis including construction costs and payback period will be conducted using actual site data.

An improved method for predicting recurrence period wind speed considering wind direction

  • Weihu Chen;Yuji Tian;Yingjie Zhang
    • Wind and Structures
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    • v.39 no.2
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    • pp.85-100
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    • 2024
  • In light of extreme value distribution probability, an improved prediction method of the Recurrence Period Wind Speed (RPWS) is constructed considering wind direction, with the Equivalent Independent Wind Direction Number (EIWDN) introduced as a parameter variable. Firstly, taking the RPWS prediction of Beijing city as an example, the traditional Cook method is used to predict the RPWS of each wind direction based on the measured wind speed data in Beijing area. On basis of the results, the empirical formulae to determine the parameter variables are fitted to construct an improved expression of the non-exceedance probability of the RPWS. In this process, the statistical model of the optimal threshold is established, and thus the independent wind speed samples exceeding the threshold are extracted and fitted to follow the Generalized Pareto Distribution (GPD) model for analysis. In addition, the Extreme Value Type I (EVT I) distribution model is used to predict and analyze the RPWS. To verify its wide applicability, the improved method is further used in cities like Jinan, Nanjing, Wuxi, Shanghai and Shenzhen to predict and analyze the RPWS of each wind direction, and the prediction results are compared against those gained via the traditional Cook method and the whole direction. Results show that the 50-year RPWS results predicted by the improved method are basically consistent with those predicted by the traditional method, and the RPWS prediction values of most wind directions are within the envelope range of the whole wind direction prediction value. Compared with the traditional method, the improved method can readily predict the RPWS under different return periods through empirical formulae, and avoid the repeated operation process and some assumptions in the traditional Cook method, and then improve the efficiency of prediction. In addition, the improved RPWS prediction results corresponding to the GPD model are slightly larger than those of the EVT I distribution model.

Shoreline-change Rates of the Barrier Islands in Nakdong River Estuary Using Aerial Photography and SPOT-5 Image (항공사진과 SPOT-5 위성영상을 이용한 낙동강 하구역 울타리섬들의 해안선 변화율)

  • Jeong, Sang-Hun;Khim, Boo-Keun;Kim, Beack-Oon;Lee, Sang-Ryong
    • Ocean and Polar Research
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    • v.35 no.1
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    • pp.1-14
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    • 2013
  • Shoreline data of the barrier islands in Nakdong River Estuary for the last three decades were assembled using six sets of aerial photographs and seven sets of satellite images. Canny Algorithm was applied to untreated data in order to obtain a wet-dry boundary as a proxy shoreline. Digital Shoreline Analysis System (DSAS 4.0) was used to estimate the rate of shoreline changes in terms of five statistical variables; SCE (Shoreline Change Envelope), NSM (Net Shoreline Movement), EPR(End Point Rate), LRR (Linear Regression Rate), and LMS (Least Median of Squares). The shoreline in Jinwoodo varied differently from one place to another during the last three decades; the west tail has advanced (i.e., seaward or southward), the west part has regressed, the south part has advanced, and the east part has regressed. After the 2000s, the rate of shoreline changes (-2.5~6.7 m/yr) increased and the east advanced. The shoreline in Shinjado shows a counterclockwise movement; the west part has advanced, but the east part has retreated. Since Shinjado was built in its present form, the west part became stable, but the east part has regressed faster. The rate of shoreline changes (-16.0~12.0 m/yr) in Shinjado is greater than that of Jinwoodo. The shoreline in Doyodeung has advanced at a rate of 31.5 m/yr. Since Doyodeung was built in its present form, the south part has regressed at the rate of -18.2 m/yr, but the east and west parts have advanced at the rate of 13.5~14.3 m/yr. Based on Digital Shoreline Analysis, shoreline changes in the barrier islands in the Nakdong River Estuary have varied both temporally and spatially, although the exact reason for the shoreline changes requires more investigation.

Molecular Cloning and Phylogenetic Analysis of PERVs from Domestic Pigs in Korea (env gene sequences) (국내 돼지에 존재하는 내인성 레트로 바이러스의 엔밸로프 유전자 클로닝 및 분자 계통학적 분석)

  • Lee, Dong-Hee;Yoo, Jae-Young;Lee, Jung-Eun;Kim, Gye-Woong;Park, Hong-Yang;Lee, Hoon-Taek;Kim, Young-Bong
    • Journal of Animal Science and Technology
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    • v.47 no.2
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    • pp.177-186
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    • 2005
  • Xenotransplantation may help to overcome the critical shortage of human tissues and organs for human transplantation, Swine represents an ideal source of such organs owing to their anatomical and physiological similarities to human besides their plentiful supply, However, the use of organs across the species barrier may be associated with the risk of transmission of pathogens, specially porcine endogenous retroviruses (PERVs).• Although most of these potential pathogens could be eliminated by pathogen-free breeding, PERVs are not eliminated by this treatment. PERVs are integrated into the genome of all pigs and produced by normal pig cells and infect human cells. They belong to gamma retroviruses and are of three classes viruses: A, B and C. In the present study, PCR based cloning was performed with chromosomal DNA extracted from pigs from domestic pigs in Korea. Amplified PCR fragments of about 1.5 Kb, covering the partial env gene, were cloned into pCR2.l-TOPO vectors and sequenced. A total of 91 env clones were obtained from domestic pigs, Berkshire, Duroc, Landrace and Yorkshire in Korea. Phylogenetic analysis of these genes revealed the presence of only PERV class A and B in the proportion of 58 % and 42 %, respectively. Among these, 28 clones had the correct open reading frame: 18 clones in class A and 10 clones in class B. Since both these PERV classes are polytropic and have the capacity to infect human cells, our data suggest that proviral PERVs have the potential to generate infectious viruses during or after xenotransplantation in human.

A Comprehensive Study on the Intake Patterns and Expenditures on Ramyun among Adults in Metropolitan Areas of Korea (수도권 거주 성인의 라면류 섭취 현황 및 실태에 관한 연구)

  • Yu, Kyung-Jin;Jung, Hyo-Sun;Yoon, Hye-Hyun
    • Culinary science and hospitality research
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    • v.19 no.1
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    • pp.204-214
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    • 2013
  • The purpose of this study is to identify instant noodle intake and consumption patterns of adults in metropolitan areas of Korea. There was a total of 702 subjects, 18 years of age and above, and data from the self-administrated questionnaires were collected and analyzed using comparative statistical analysis, including frequency, chi-square, t-test, and one-way ANOVA. According to the results, preference level for instant noodles among the adults was indicated to be $4.84{\pm}1.32$ and 54% of subjects either "liked" or "more than liked" noodles. People in 20s liked them the most. Noodles in envelope type package with soup are highly preferred. The most common reason why they chose to eat instant noodles was "convenience." At the same time, people avoid them because of their bad nutrition and calories. Half of the subjects consume instant noodles at least once a week mainly for lunch and dinner as a substitute for their meal. In the case of eating-together-with-family was the largest. 72.4% of subjects consumed entire soup additive content and 20.6% of them eat almost without soup because of being full and worrying that it might be harmful to health. Significant difference was shown in the preference level and intake patterns depending on respondents' general characteristics. Limitations and future research directions are also discussed.

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The study of nondestructive evaluation method of paper records materials by NIR spectroscopy (근적외선 분광분석을 이용한 종이기록물의 비파괴 특성평가 연구)

  • Han, Yoon-Hee;Shin, Yong-Min;Park, Soung-Be;Nam, Sung-Un;Kim, Hyo-Jin
    • Analytical Science and Technology
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    • v.23 no.3
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    • pp.304-311
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    • 2010
  • Near Infrared Spectroscopy (NIRs) has been applied for rapid and nondestructive paper measurement by replacing the current destructive method to the property of paper. Current standard methods for the property of paper were pH, moisture, breaking length, and folding endurance, which data were compared with spectrum of FT-NIR spectrometer. Various paper products such as copy, envelope, white, newspaper, as well as old paper produced around 1960~1980 were used as the sample. The correlation ($R^2$) and standard error of prediction (SEP) results for breaking length, folding endurance, moisture and pH are $R^2$=0.914, SEP=0.508, $R^2$=0.926, SEP=0.281, $R^2$=0.941, SEP=0.931, pH $R^2$=0.949, SEP= -0.0631, respectively. This result show that NIRs can be applied to practical application for nondestructive analysis of paper records materials.

PERIOD CHANGES OF W UMa TYPE CONTACT BINARY SS ARIETIS (W UMa형 접촉쌍성 SS ARIETIS의 공전주기 변화)

  • 김천휘;한원용;윤재혁;나일성
    • Journal of Astronomy and Space Sciences
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    • v.14 no.1
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    • pp.44-58
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    • 1997
  • The BVR CCD photometric observations of W UMa-type eclipsing binary SS Ari were made on ten nights from November 1996 to December 1996. Eight new times of minimum lights were derived. The analysis of times of minima of SS Ari confirms the suggestions of other previous investigators that the orbital period of SS Ari have been suffering from a sinusoidal varition. The amplitude and period for the cyclic period changes were calculated as about $58^{y}$ and $0.^{d}053$, respectively. The period variation has been discussed in terms of two potential mechanisms: 1) the light-time effect due to a hypothetical third body and 2) deformations in the convective envelope of a magnetically active component. In the earlier case, the third body has a mass of $1.3M_{\odot}$, if exist, in the form of a white dwarf or a binary system. It seems that the system velocities from the spectroscopic observations supports this interpretation. Meanwhile in the latter case, the primary component is mainly responsible for the magnetic activity of this system with a theoretical amplitude of $\pm0.^{m}08$. However, we cannot make a conclusion which is reasonable explanation at this point, due to lack of observational data. Moreover, the period variation of SS Ari shows duplication about $14^y$, cyclic period with an amplitude of about $0.^d001$ to the above periodic change. We also cannot make an acceptable conclusion for it at this time.

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