• Microbiology and Biotechnology Letters
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• v.31 no.2
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• pp.117-123
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• 2003

For screening of autoregulator receptor gene from Saccharopolyspora erythraea, PCR was performed with primers of receptor gene designed on the basis of amino acid sequences of autoregulator receptor proteins with known function. PCR products were subcloned into the BamHI site of pUC19 and transformed into the E. coli DH5$\alpha$. The isolated plasmid from transformant contained the fragment of 120 bp, which was detected on 2% gel after BamHI treatment. The insert, 120 bp PCR product, was confirmed as the expected internal segment of gene encoding autoregulator receptor protein by sequencing. Southern and colony hybridization using Saccha. erythraea chromosomal DNA were performed with the insert as probe. The plasmid (pEsg) having 3.2 kbp SacI DNA fragment from Saccha. erythraea is obtained. The 3.2 kbp SacI DNA fragment was sequenced by the dye terminator sequencing. The nucleotide sequence data was analyzed with GENETYX-WIN (ver 3.2) computer program and DNA database. frame analyses of the nucleotide sequence revealed a gene encoding autoregulator receptor protein which is a region including KpnI and SalI sites on 3.2 kbp SacI DNA fragment. The autoregulator receptor protein consisting of 205 amino acid was named EsgR by author. In comparison with known autoregulator receptor proteins, homology of EsgR showed above 30%.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.8
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• pp.1-8
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• 2020

Air ducts installed for ventilation inside buildings accumulate contaminants during their service life. Robots are installed to clean the air duct at low cost, but they are still not fully automated and depend on manpower. In this study, an intersection detection algorithm for autonomous driving was applied to an air duct cleaning robot. Autonomous driving of the robot was achieved by calculating the distance and angle between the extracted point and the center point through the intersection detection algorithm from the camera image mounted on the robot. The training data consisted of CAD images of the duct interior as well as the cross-point coordinates and angles between the two boundary lines. The deep learning-based CNN model was applied as a detection algorithm. For training, the cross-point coordinates were obtained from CAD images. The accuracy was determined based on the differences in the actual and predicted areas and distances. A cleaning robot prototype was designed, consisting of a frame, a Raspberry Pi computer, a control unit and a drive unit. The algorithm was validated by video imagery of the robot in operation. The algorithm can be applied to vehicles operating in similar environments.

• Journal of the Korean Institute of Navigation
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• v.23 no.1
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• pp.75-84
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• 1999

Although many studies on modelling of port competitive situation have been conducted, both theoretical frame and methodology are still very weak. In this study, therefore, a new algorithm called ESD (Extensional System Dynamics) for the evaluation of port competition was presented, and applied to simulate port systems in northeast asia. The detailed objectives of this paper are to develop Unit fort Model by using SD(System Dynamics) method; to develop Competitive Port Model by ESD method; to perform sensitivity analysis by altering parameters, and to propose port development strategies. For these the algorithm for the evaluation of part's competition was developed in two steps. Firstly, SD method was adopted to develop the Unit Port models, and secondly HFP(Hierarchical Fuzzy Process) method was introduced to expand previous SD method. The proposed models were then developed and applied to the five ports - Pusan, Kobe, Yokohama, Kaoshiung, Keelung - with real data on each ports, and several findings were derived. Firstly, the extraction of factors for Unit Port was accomplished by consultation of experts such as research worker, professor, research fellows related to harbor, and expert group, and finally, five factor groups - location, facility, service, cargo volumes, and port charge - were obtained. Secondly, system's structure consisting of feedback loop was found easily by location of representative and detailed factors on keyword network of STGB map. Using these keyword network, feedback loop was found. Thirdly, for the target year of 2003, the simulation for Pusan port revealed that liner's number would be increased from 829 ships to 1,450 ships and container cargo volumes increased from 4.56 million TEU to 7.74 million TEU. It also revealed that because of increased liners and container cargo volumes, length of berth should be expanded from 2,162m to 4,729m. This berth expansion was resulted in the decrease of congested ship's number from 97 to 11. It was also found that port's charge had a fluctuation. Results of simulation for Kobe, Yokohama, Kaoshiung, Keelung in northeast asia were also acquired. Finally, the inter port competition models developed by ESB method were used to simulate container cargo volumes for Pusan port. The results revealed that under competitive situation container cargo volume was smaller than non-competitive situation, which means Pusan port is lack of competitive power to other ports. Developed models in this study were then applied to estimate change of container cargo volumes in competitive relation by altering several parameters. And, the results were found to be very helpful for port mangers who are in charge of planning of port development.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.3
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• pp.286-292
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• 2005

This study is to investigate the industrial boiler which can be significantly affected by the restriction of NOx. Note that the application of SNCR method to industrial boiler is usually blown as not feasible due to the insufficient residence time for proper mixing. The purpose of this study is to investigate the applicability of the SNCR system application to the industrial boiler, which produces 40 tons of steam per hour using heavy oil. For the industrial boiler with 3-D rectangular coordinate, the general coding are made fur various turbulence modeling such as turbulent flow, turbulent fuel combustion, thermal NO formation and destruction together with the NO reaction with reducing agents. Further, the incorporation of drop trajectory model is successfully made in 3-D rectangular coordinate with Lagrangian frame and the main swirl burner effect on the characteristics of flame is considered. As expected a short flame was created and thereby NOx is removed more efficiently by increasing the proper region of temperature for NO reduction reaction. The validation of program was made successfully by the comparison of experimental data. Based on the reliable calculation results, the SNCR method in a industrial boiler shows the possibility as one of viable NO reduction method by the use of well designed mixing air of reducing agent.

• Journal of KIISE:Software and Applications
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• v.35 no.11
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• pp.671-680
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• 2008

Motion capture devices have been utilized in producing several contents, such as movies and video games. However, since motion capture devices are expensive and inconvenient to use, motions segmented from captured data was recycled and synthesized to utilize it in another contents, but the motions were generally segmented by contents producers in manual. Therefore, automatic motion segmentation is recently getting a lot of attentions. Previous approaches are divided into on-line and off-line, where ow line approaches segment motions based on similarities between neighboring frames and off-line approaches segment motions by capturing the global characteristics in feature space. In this paper, we propose a graph-based high-level motion segmentation method. Since high-level motions consist of repeated frames within temporal distances, we consider similarities between neighboring frames as well as all similarities among all frames within the temporal distance. This is achieved by constructing a graph, where each vertex represents a frame and the edges between the frames are weighted by their similarity. Then, normalized cuts algorithm is used to partition the constructed graph into several sub-graphs by globally finding minimum cuts. In the experiments, the results using the proposed method showed better performance than PCA-based method in on-line and GMM-based method in off-line, as the proposed method globally segment motions from the graph constructed based similarities between neighboring frames as well as similarities among all frames within temporal distances.

• KIPS Transactions on Software and Data Engineering
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• v.1 no.3
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• pp.177-186
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• 2012

In this paper, we propose a real-time approach for detecting and tracking a hand region by analyzing depth images. We build a hand model in advance. The model has the shape information of a hand. The detecting process extracts out moving areas in an image, which are possibly caused by moving a hand in front of a camera. The moving areas can be identified by analyzing accumulated difference images and applying the region growing technique. The extracted moving areas are compared against a hand model to get justified as a hand region. The tracking process keeps the track of center points of hand regions of successive frames. For this purpose, it involves three steps. The first step is to determine a seed point that is the closest point to the center point of a previous frame. The second step is to perform region growing to form a candidate region of a hand. The third step is to determine the center point of a hand to be tracked. This point is searched by the mean-shift algorithm within a confined area whose size varies adaptively according to the depth information. To verify the effectiveness of our approach, we have evaluated the performance of our approach while changing the shape and position of a hand as well as the velocity of hand movement.

• Korean Journal of Applied Biomechanics
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• v.18 no.4
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• pp.99-107
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• 2008

The aim of this study was to provide fundamental data in training to improve athletes' competitiveness through the comparative analysis of kinematic variables according to the types of stance. For this study, the subjects selected 4 Junior Weight lifters. Subjects performed two type(8-type and 11-type) Dead-lift and their performance was sampled at 60frame/sec. using four high-speed digital video cameras. After digitizing images from four cameras, the two-dimensional coordinates were used to produce three-dimensional coordinates of the 15 body segments(20 joint makers and 2 bar makers). And the results were as follows. 1. As for the time required for stances, 8-type motion was faster than 11-type motion. 2. As for the body-center shift in stances, 8-type motion was bigger than 11-type motion in back and forth motion shift, and 11-type motion was bigger than 8-type motion in right and left, up and down motion shift. 3. As for the speed of a body-center and a babel, 8-type motion was faster than 11-type motion. 4. As for the motion-trace of a babel in stances, 8-type motion was bigger than 11-type in back and forth, right and left motion and 11-type motion was bigger than 8-type in up and down motion. 5. As for the body-angles in stances, 8-type motion was bigger than 11-type in the stance angle, and 11-type motion is bigger than 8-type in the angles of a coxa, a knee and an ankle. As a result of the comparative analysis between 8-type and 11-type stance of Junior Weight lifters dead-lift, both were generally similar in variables, but 8-type motion was more stable than 11-type in aspects of time, speed, center shift, angle change.

• Nuclear Engineering and Technology
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• v.46 no.3
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• pp.439-446
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• 2014

An amphibious inspection robot system (hereafter AIROS) is being developed to visually inspect the in-containment refueling storage water tank (hereafter IRWST) strainer in APR1400 instead of a human diver. Four IRWST strainers are located in the IRWST, which is filled with boric acid water. Each strainer has 108 sub-assembly strainer fin modules that should be inspected with the VT-3 method according to Reg. guide 1.82 and the operation manual. AIROS has 6 thrusters for submarine voyage and 4 legs for walking on the top of the strainer. An inverse kinematic algorithm was implemented in the robot controller for exact walking on the top of the IRWST strainer. The IRWST strainer has several top cross braces that are extruded on the top of the strainer, which can be obstacles of walking on the strainer, to maintain the frame of the strainer. Therefore, a robot leg should arrive at the position beside the top cross brace. For this reason, we used an image processing technique to find the top cross brace in the sole camera image. The sole camera image is processed to find the existence of the top cross brace using the cross edge detection algorithm in real time. A 5-DOF robot arm that has multiple camera modules for simultaneous inspection of both sides can penetrate narrow gaps. For intuitive presentation of inspection results and for management of inspection data, inspection images are stored in the control PC with camera angles and positions to synthesize and merge the images. The synthesized images are then mapped in a 3D CAD model of the IRWST strainer with the location information. An IRWST strainer mock-up was fabricated to teach the robot arm scanning and gaiting. It is important to arrive at the designated position for inserting the robot arm into all of the gaps. Exact position control without anchor under the water is not easy. Therefore, we designed the multi leg robot for the role of anchoring and positioning. Quadruped robot design of installing sole cameras was a new approach for the exact and stable position control on the IRWST strainer, unlike a traditional robot for underwater facility inspection. The developed robot will be practically used to enhance the efficiency and reliability of the inspection of nuclear power plant components.

• Journal of Wetlands Research
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• v.9 no.1
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• pp.79-89
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• 2007

This paper provides a comprehensive overview of the present status of several environmental problems caused by emerging toxic substances such as persistent organic pollutants (POPs), endocrine disrupting chemicals (EDCs), and arsenic in various environmental media in Vietnam. Monitoring data reported during the 1990s demonstrated elevated contamination of DDTs in most of these compartments in Vietnam. Studies in frame of the Asia-Pacific Mussel Watch Program revealed that fish, mussels and resident birds from Vietnam contained higher concentrations of DDTs as compared to other countries in region, suggesting the role of Vietnamese environment as a significant emission source of DDT in the Southeast Asian region. The estimated dietary intakes of PCBs and DDTs for Vietnamese were relatively high among Asian developing countries, suggesting potential risk for humans posed by thesechemicals. Widespread contamination of some endocrine active compounds such as alkylphenols and phthalates was observed at various sites along the coasts of northern and middle Vietnam. The presence of significant source of bisphenol-A along Red River estuary was revealed with the concentrations comparable to those reported for developed nations. A case study on seasonal variation of alkylphenols and phthalates in surface water of river delta and estuary of north and middle Vietnam indicated the differences in distribution of these compounds between dry and rainy seasons. Higher concentrations of alkylphenols and phthalates were found in dry season in estuary; while the contrasting pattern was observed in the river delta, showing elevated residues in rainy season. This result suggests the different behavior of alkylphenols and phthalates in river delta and coastal environment. From ecotoxicological perspectives, concentrations of bis-phenol A and di(2-ethylhexyl)phthalates [DEHP] in surface water from some locations in Vietnam exceeded the guideline values for Ecotoxicological Effects and the Environmental Risk Limit, respectively, suggesting potential for toxic implications on aquatic wildlife. Widespread and elevated arsenic contamination was discovered inour recent surveys in groundwater in a large area of suburban areas of Hanoi city, the capital of Vietnam. The most recent investigation in 4 villages showed about more than 50 % of groundwater samples contained As concentrations exceeding 50 g/L (the WHO and Vietnamese standard). In particular, in Son Dong villages, 58 % of samples analyzed contained As concentrations higher than 200 g/L. Good correlations were found in As concentrations in water and hair and urine of peoples in corresponding families, suggesting the chronic exposure to As by people living in As-contaminated ground water areas. In Son Dong village, As levels in hair (mean: 1.7 mg/kg dry wt) and urine (g/g creatinine) exceeding the reference values recommended by WHO, suggesting potential for human risk posed by long term accumulation of As in human body. Future studies should be focused on the time trends of POPs and EDCs in biota in Vietnam in order to predict future trend of contamination and to reveal new clues for understanding possible toxic impacts on aquatic organisms. The issues of arsenic contamination in groundwater and their chronic toxic implications on human health should be systematically investigated in the future.

• Korean Journal of Plant Resources
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• v.20 no.2
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• pp.155-167
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• 2007

This study was carried out to offer the raw data for environment conservation by surveying and analysing the flora distributed over Jinhae-si, and to present the frame for preparing the method of obtaining the bioresource in approach to plant resource. The period of survey was from May, 2005 to June, 2006, and the routes were A(Mt. Jangbok), B(Ahnmin hill) C(Mt. Hwa). The results as follows. The vascular plants were summarized as 447 taxa; 98 families, 286 genera, 394 species, 49 varieties, and 4 forma. The rare plants designated by Korea Forest Service were 2 taxa; Tricyrtis dilatata and Viola albida. The Korean endemic plants were 11 taxa; Tricyrtis dilatata, Salix caprea, Carpinus coreana, Melandryum seoulense, Clematis trichotoma, Thalictrum uchiyamai, Deutzia coreana, Spiraea prunifolia for. simpliciflora, Lespedeza maritima, Forsythia koreana and Weigela subsessilis.