• Korean Journal of Veterinary Research
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• v.39 no.1
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• pp.90-95
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• 1999

A species-specific 760 base pair(bp) BamHI to EcoRI DNA fragment(fMG-2) of lipoprotein gene was isolated from a Mycoplasma gallisepticum(M gallisepticum) genomic library. Based on the DNA sequence data of fMG-2, a pair of 25bp primers was synthesized. When used in the polymerase chain reaction(PCR), 732bp DNA products were amplified from 6 standard strains and 10 field isolates of M gallisepticum, but not from 2 Mycoplasma synoviae and 7 other Mycoplasma species. The lower detection limit was 100fg of the genomic DNA. Identity of the PCR products was confirmed by comparison of patterns of restriction endonuclease analysis with AseI, DraI, EcoRV and SspI.

• The Journal of Information Technology and Database
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• v.4 no.2
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• pp.3-19
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• 1998

관계형 데이터베이스 성능을 향상시키는데 중요한 요소 중의 하나는 트랜잭션을 처리하기 위해 데이터를 디스크에서 주 기억장치로 옮기는데 필요한 디스크 액세스(access) 횟수이다. 본 연구는 관계형 데이터베이스에서 트랜잭션을 처리할 때, 릴레이션(relation)을 속성의 중복할당을 허용하여 분할하고, 디스크에 단편(fragment)으로 저장하므로써 필요한 단편만을 액세스하여 디스크의 액세스 횟수를 줄이는 방법을 연구하였다. 본 연구에서는 속성의 중복할당을 허용하여 디스크의 액세스 횟수를 최소화시킬 수 있는 수직분할문제에 수리모형을 조회, 갱신트랙잭션을 모두 고려하여 0-1 정수계획법으로 개발하였다. 또한 모형에 대한 최적해법으로 분지한계법을 제안하였으며, 분지한계법으로 큰 문제를 푸는데는 많은 시간이 소요되므로 계산량을 줄일 수 있는 초기처리방법과 비용계산방법을 제안하였다. 속성의 중복을 허용하여 구한 해가 중복을 고려하지 않은 경우의 해보다 디스크 액세스횟수가 감소한 것으로 나타났으며, 갱신트랜？션의 수가 증가함에 따라 중복되는 속성의 수가 감소하는 결과를 나타내었다.

• Bulletin of the Korean Chemical Society
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• v.31 no.11
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• pp.3318-3326
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• 2010

Five new series of 3,4-ethylenedioxythiophene derivatives carrying important pharamacophores, viz., amide, ester, ether and active secondary aryl moieties have been designed and synthesized through multistep reactions starting from thiodiglycolic ester and diethyl oxalate. They have been characterized by elemental and spectral data. All the target compounds have been screened for their anticonvulsant activity at three different models viz. maximal electroshock (MES), subcutaneous metrazole (scMET), and 6 Hz screen and evaluated for their neurotoxicity in rotorod model. Compound 6a emerged as lead with no neurotoxicity. All the five series of compounds are safe in the toxicity studies at the maximum dose of 300 mg/kg of body weight. Amongst the tested compounds, the ester pharmacophore with thioamide fragment has showed better activity than the other analogs.

• Korean Journal of Crystallography
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• v.11 no.2
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• pp.84-88
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• 2000

The crystal of cholesteryl crotylcarbonate was investigated by X-ray diffraction method. Crystallographic data for the title compound: P21, a=13.510(2)Å, b=11.843(2)Å, c=19.882(2)Å, β=106.88(1)°, Z=4. Reflections were collected with an Enraf-Nonius CAD-4 diffractometer equipped with a graphite monochromator. The structure was solved by direct methods and refined by least-squares analyses. The final R value was 0.125 for 2607 reflections. The cholesterol fragment of the title compound were in good agreement with those for related cholesterol derivatives. The molecules were stacked in clearly separated layers. At the center of the layers, there were cholesteryl-cholesteryl interactions between the symmetry-related B molecules and the cholesteryl-C(17) side chain of A molecules. There were also interactions between the C(17) side chain of B molecules and the crotylcarbonate chain in the interface region between layers. The crystal structure of the title compound turned out to be isostructural with those of cholesteryl ethylcarbonate and cholesteryl propylcarbonate.

• Mycobiology
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• v.39 no.2
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• pp.71-78
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• 2011

The phylogenetic relationships of the most dominant and morphologically cryptic endophytic fungal isolates from each of five selected medicinal plants, namely Potentilla fulgens, Osbeckia stellata, Osbeckia chinensis, Camellia caduca, and Schima khasiana of the biodiversity rich state of Meghalaya, were assessed with random amplification of polymorphic DNA and PCR-restriction fragment length polymorphism profiles. Sequencing of the internal transcribed spacer 1, small subunit rRNA and partial ${\beta}$-tubulin gene fragments was also conducted to determine the phylogenetic relationships of these isolates with fungal sequences available in Genbank, NCBI. The identity of the fungal isolates is suggested based on the molecular phylogenetic data.

• Genomics & Informatics
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• v.4 no.2
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• pp.65-70
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• 2006

Peptide mass mapping is the matching of experimentally generated peptides masses with the predicted masses of digested proteins contained in a database. To identify proteins by matching their constituent fragment masses to the theoretical peptide masses generated from a protein database, the peptide mass fingerprinting technique is used for the protein identification. Thus, it is important to know the theoretical mass distribution of the database. However, few researches have reported the peptide mass distribution of a database. We analyzed the peptide mass distribution of non-redundant protein sequence database in the NCBI after digestion with 15 different types of enzymes. In order to characterize the peptide mass distribution with different digestion enzymes, a power law distribution (Zipfs law) was applied to the distribution. After constructing simulated digestion of a protein database, rank-frequency plot of peptide fragments was applied to generalize a Zipfs law curve for all enzymes. As a result, our data appear to fit Zipfs law with statistically significant parameter values.

• Biomolecules & Therapeutics
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• v.8 no.3
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• pp.217-222
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• 2000

Bacterial components and their derivatives have been reported to mediate various immunomodulating activities and to activate immune cells including macrophage. In this study, the secretory and cellular macrophage response to teichoicated fragments (TFs) from pneumococcal cell wall subcomponent were examined. Tumoricidal activity was measured by MTT assay and secretory molecules were assessed by biological assay. After stimulation of macrophages with various doses of TFs for 18hrs, secretion of TNF-$\alpha$, nitrite and $H_2O$$_2$ were significantly increased as compared to medium-treated control. In addition, tumorcidal activity of TFs-treated macrophages was enhanced, whereas production of IL-1 and IL-6, and phagocytic activity were not induced. These data suggest that TFs is a potent inducer of macrophage secretory and cellular activities.

• Molecules and Cells
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• v.44 no.12
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• pp.883-892
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• 2021

Genome-wide chromosome conformation capture (3C)-based high-throughput sequencing (Hi-C) has enabled identification of genome-wide chromatin loops. Because the Hi-C map with restriction fragment resolution is intrinsically associated with sparsity and stochastic noise, Hi-C data are usually binned at particular intervals; however, the binning method has limited reliability, especially at high resolution. Here, we describe a new method called HiCORE, which provides simple pipelines and algorithms to overcome the limitations of single-layered binning and predict core chromatin regions with three-dimensional physical interactions. In this approach, multiple layers of binning with slightly shifted genome coverage are generated, and interacting bins at each layer are integrated to infer narrower regions of chromatin interactions. HiCORE predicts chromatin looping regions with higher resolution, both in human and Arabidopsis genomes, and contributes to the identification of the precise positions of potential genomic elements in an unbiased manner.

• BMB Reports
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• v.36 no.5
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• pp.427-432
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• 2003

Jute is the principal coarse fiber for commercial production and use in Bangladesh. Therefore, the development of a high-yielding and environmental-stress tolerant jute variety would be beneficial for the agro economy of Bangladesh. Two molecular fingerprinting techniques, random-amplified polymorphic DNA (RAPD) and amplified-fragment length polymorphism (AFLP) were applied on six jute samples. Two of them were cold-sensitive varieties and the remaining four were cold-tolerant accessions. RAPD and AFLP fingerprints were employed to generate polymorphism between the cold-sensitive varieties and cold-tolerant accessions because of their simplicity, and also because there is no available sequence information on jute. RAPD data were obtained by using 30 arbitrary oligonucleotide primers. Five primers were found to give polymorphism between the varieties that were tested. AFLP fingerprints were generated using 25 combinations of selective-amplification primers. Eight primer combinations gave the best results with 93 polymorphic fragments, and they were able to discriminate the two cold-sensitive and four cold-tolerant jute populations. A cluster analysis, based on the RAPD and AFLP fingerprint data, showed the population-specific grouping of individuals. This information could be useful later in marker-aided selection between the cold-sensitive varieties and cold-tolerant jute accessions.

• Proceedings of the Korean Society for Language and Information Conference
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• 2002.02a
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• pp.2-27
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• 2002

As pact of a long-ranged project that aims at establishing database-theoretic semantics as a model of computational semantics, this presentation focuses on the development of a syntactic component for processing strings of words or sentences to construct semantic data structures. For design arid modeling purposes, the present treatment will be restricted to the analysis of some problematic constructions of Korean involving semi-free word order, conjunction arid temporal anchoring, and adnominal modification and antecedent binding. The present work heavily relies on Hausser's (1999, 2000) SLIM theory for language that is based on surface compositionality, time-linearity arid two other conditions on natural language processing. Time-linear syntax for natural language has been shown to be conceptually simple and computationally efficient. The associated semantics is complex, however, because it must deal with situated language involving interactive multi-agents. Nevertheless, by processing input word strings in a time-linear mode, the syntax cart incrementally construct the necessary semantic structures for relevant queries and valid inferences. The fragment of Korean syntax will be implemented in Malaga, a C-type implementation language that was enriched for both programming and debugging purposes arid that was particluarly made suitable for implementing in Left-Associative Grammar. This presentation will show how the system of syntactic rules with constraining subrules processes Korean sentences in a step-by-step time-linear manner to incrementally construct semantic data structures that mainly specify relations with their argument, temporal, and binding structures.