Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Detection of Mycoplasma gallisepticum using Polymerase Chain Reaction(PCR)

PCR 기법을 이용한 Mycoplasma gallisepticum의 검출

  • Lee, Young-ju (National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forest) ;
  • Kim, Ki-seuk (National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forest) ;
  • Kim, Jong-wan (National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forest) ;
  • Tak, Ryun-bin (College of Veterinary Medicine Kyungpook National University)
  • 이영주 (농림부 국립수의과학검역원) ;
  • 김기석 (농림부 국립수의과학검역원) ;
  • 김종완 (농림부 국립수의과학검역원) ;
  • 탁연빈 (경북대학교 수의과대학)
  • Received : 1998.05.02
  • Published : 1999.03.22
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Abstract

A species-specific 760 base pair(bp) BamHI to EcoRI DNA fragment(fMG-2) of lipoprotein gene was isolated from a Mycoplasma gallisepticum(M gallisepticum) genomic library. Based on the DNA sequence data of fMG-2, a pair of 25bp primers was synthesized. When used in the polymerase chain reaction(PCR), 732bp DNA products were amplified from 6 standard strains and 10 field isolates of M gallisepticum, but not from 2 Mycoplasma synoviae and 7 other Mycoplasma species. The lower detection limit was 100fg of the genomic DNA. Identity of the PCR products was confirmed by comparison of patterns of restriction endonuclease analysis with AseI, DraI, EcoRV and SspI.

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