• Proceedings of the Korean Society of Applied Pharmacology
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• 1996.04a
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• pp.277-277
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• 1996

Anti-CALLA Fab-RTA immunotoxins were constructed using two crosslinking agent, SMPT and SMCC, to generate a disulfide and a thioether bridge between Fab fragment of K269-65 MoAb and RTA toxin moieties, respectively. These immunotoxins were selectively immunoreative with CALLA$\^$+/ B-lineage Daudi cells. SMPT and SMCC mediated RTA immunotoxins were prepared with 49% and 53% of the RTA conjugation yields, respectively. SDS-PAGE analysis show that immunotoxins were constructed with major Fab-1 RTA (76kda), minor Fab-2RTA (106kda) and Fab-3RTA (136kda) compositions. The breakdown rates of immunotoxins were determined in the presence of glutathione by measuring the amount of reduced immunotoxins using size-exclusion HPLC. The SMCC immunotoxins were more resistant to the glutathione than SMPT immunotoxins. But, our data showed that the SMPT mediated disulfide bonded immunotoxins were much more active than the SMCC mediated thioether bonded immunotoxins to kill the target cells in vitro.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 1999.05a
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• pp.104-108
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• 1999

It is presented many effect that represented by implicated one image more than each images with a fragment meaning. ‘Mosaic’ is technique be applied at this situation. ‘Mosaic’ is created by complicated one new image which by multi image be eliminated overlap region. This research is development for mosaic system by multi image. The system is divided that shot segmentation and mosaic image creation. Shot segmentation divided that merge with respect to similarity images which video data of moving picture in sequence time and mosaic image creation is composed of one image with which all frames in segmented shot.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.126-126
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• 2018

Many of researches have revealed that human intestinal microbiota is related to health. Several diseases like obesity, diabetes, and hypertension are affected by the microbiota directly and indirectly. So, interventions with food and drug have been tried to change a composition of the microbiota to better condition. However, few natural medicines have elucidated to date. To understand an influence on microbiota by plant materials including Glycyrrhizae Radix, the extract of medicines were administered to mice and the feces were collected before and after the administration. The feces were analyzed by terminal restriction fragment length polymorphism (T-RFLP). The changes in composition of mice gut microbiota were detected and analyzed. The data could be utilized to further study about biological activities of the plant medicines.

• Fisheries and Aquatic Sciences
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• v.4 no.1
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• pp.25-31
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• 2001

4-Aminobutyrate aminotransferase plays an essential role in the 4-aminobutyric acid shunt, converting 4-aminobutyrate to succinic semi aldehyde. We isolated and sequenced' a fish cDNA fragment that encodes 4-aminobutyrate aminotransferase. A brain cDNA library from flounder (Paralichthys olivaceus) was constructed using the ZAP- III XR vector and screened for the fish 4-aminobutyrate aminotransferase gene using a probe derived from the conserved sequences of known mammalian 4-aminobutyrate aminotransferases. A partial cDNA for 4-aminobutyrate aminotransferase was cloned and found to be 700 bp in length corresponding to 66 amino acids. Nucleotide sequence of the clone was aligned with NCBI (National Center for Biotechnology Information) DNA sequence data base. The result showed high sequence identity with previously reported mammalian 4-aminobutyrate aminotransferases. The trans­criptional level of flounder 4-aminobutyrate aminotransferase was detected with the presence of mRNA at different flounder tissues by reverse transcription-polymerase chain reaction (RT-PCR). The expression of flounder 4-aminobutyrate aminotransferase was also tested and detected from the flounder tissues of the brain, liver, kidney and pancreas.

• 한국HCI학회:학술대회논문집
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• 2007.02a
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• pp.1025-1030
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• 2007

여러 학계와 산업계로부터 인체영상과 같은 정적인 볼륨 데이터뿐만 아니라, 유체 흐름과 같은 동적으로 움직이는 Time-Variant 볼륨 데이터에 대한 실시간 렌더링의 요구가 계속되고 있다. 일반적으로 Time-Variant 데이터는 그 크기가 정적 볼륨 데이터의 수배에서 수백 배에 이르러, 이를 실시간으로 가시화하는 데에 많은 어려움이 있어왔다. 한편, PC 그래픽스 하드웨어의 급격한 발전에 따라 슈퍼컴퓨터나 다수의 컴퓨터들을 이용한 병렬/분산 렌더링으로나 가능했던 Time-Variant 볼륨 데이터의 실시간 볼륨 렌더링을 한대의 일반 PC에서 수행하려는 시도가 계속되고 있다. GPU의 꼭지점 및 프래그먼트 쉐이더(vertex & fragment shader)는 수치 계산에 최적화된 벡터 연산과 사용자 프로그래밍 기능으로 빠른 볼륨 렌더링을 일반 PC에서도 가능하게 했다. 본 논문에서는 GPU를 이용해서 Time-Variant 볼륨 데이터를 빠르게 가시화하고, 이렇게 개발한 GPU 볼륨 렌더링 프로그램을 사용자가 사용하기 편리하도록 사용자 친화적인 유저 인터페이스를 설계하고 구현하였다. 특히, 시간에 따라 동적으로 변화해야 하는 전이함수를 최대한 편리하게 생성할 수 있도록 전이함수 에디터에 중점을 두었다.

• Journal of the Korean Society of Costume
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• v.53 no.4
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• pp.31-47
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• 2003

The purpose of this study was to investigate the Digital Paradigm cords depicted on the Digital Clothing by borrowing nearby fields. Therefore, it will offer not only theoretical data but also databases for product development on functional and aesthetic views even though it is not systematically set up the idea vet. The Digital Paradigm cords of Interactivity. Nonlinearity and Combination in the Digital Clothing were determined. This study found that Interactivity Paradigm cord was expressed by the interaction of clothing and computer, the techniques of built in·attach·detach·fragment of the Digital Clothing at the external indicators, and maintained this personalizing that was pursued comprehension and the shape and silhouette of the existed clothing, that Nonlinearity Paradigm cord was able to overcome the limitation of a successively approach, in other words the discontinuous moving possibilities of time and space, that Combination Paradigm cord was contained creative concept without fixed ideas through coordination of Digital Clothing that was combination. continuous creation, changes and process between heterogeneous factors. In conclusion, introduction of the new Digital Paradigm is going to accelerate on lasting development and research.

• Proceedings of the Korean Society of Medical Physics Conference
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• 2002.09a
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• pp.211-213
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• 2002

The purpose of this work is acquiring some parameters of therapeutic heavy ion beams after penetrating a thick target. The experiments were performed using a pencil-like $\^$12/C beam of about 3 mm in diameter from NIRS-HIMAC, and the data were taken at several points of the target thickness for $\^$12/C beam of 290 MeV/u and 400 MeV/u. By the simultaneous measurements using some detectors, the atomic number of each fragment particle was identified, and the beam profile, the dose distribution and the LET spectrum for each element were derived.

• Journal of Microbiology and Biotechnology
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• v.9 no.6
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• pp.732-736
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• 1999

The superoxide dismutase (SOD) in Lactococcus lactis was measured quantitatively and qualitatively under various culture conditions. The L. lactis SOD was induced by oxidative stress. As the concentration of paraquat to produce superoxide radicals increased, the growth of L. lactis decreased with concomitant increase of SOD activity. The SOD activity was found to be growth-phase dependent: when aerobically grown cells entered to the stationary phase, the activity increased gradually until the late stationary phase. From inhibition studies, L. lactis SOD was found to be insensitive to KCN and $H_2O_2$ which are known to inhibit Cu/ZnSOD and FeSOD, respectively. Moreover, as the concentration of manganese in the medium increased, the activity of SOD also increased. These data strongly suggested that L. lactis possessed a single manganese-containing SOD (MnSOD). Finally, a putative sod gene fragment of 510 bp was identified in L. lactis using a polymerase chain reaction (PCR) with degenerate primers designed from the deduced DNA sequences of known SOD genes.

• BMB Reports
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• v.31 no.2
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• pp.196-200
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• 1998

Zeins, maize storage proteins, are retained in the endoplasmic reticulum (ER) during the subcellular targeting process without the ER retention signal. Circumstantial data indicate that the 27K zein is an ER transmembrane protein. The potential transmembrane domain may permit the 27K zein to remain in the ER. This study investigated the potential transmembrane feature by employing alkaline extraction, proteinase K digestion, and surface biotinylation on isolated intact protein bodies. These assays consistently support the possibility of the 27K zein as a transmembrane protein. The 27K zein polypeptide was shown to be associated with alkali-stripped membranes. The polypeptide was digested by proteinase K to a smaller fragment. According to surface biotinylation, the 27K zeins was labeled to the exclusion of other classes of zeins. This study, therefore, concludes that the 27K zein has an ER transmembrane domain, which may serve as an anchor for zeins' ER retention.

• Journal of Microbiology and Biotechnology
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• v.22 no.4
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• pp.563-566
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• 2012

To investigate the possibility of horizontal gene transfer between agricultural microorganisms and soil microorganisms in the environment, Bacillus subtilis KB producing iturin and the PGPR recombinant strain Pseudomonas fluorescens MX1 were used as model microorganisms. The soil samples of cucumber or tomato plants cultivated in pots and the greenhouse for a six month period were investigated by PCR, real-time PCR, Southern hybridization, and terminal restriction fragment length polymorphism (T-RFLP) fingerprinting. Our data from Southern blotting and T-RFLP patterns suggest that the model bacteria do not give significant impacts on the other bacteria in the pots and greenhouse during cultivation.