• Natural Product Sciences
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• 제20권2호
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• pp.80-85
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• 2014

In the course of screening for antioxidant compounds from natural plants in Korea by measuring the radical scavenging effect, a methanol extract of the flower buds of Daphne genkwa S. et Z. (Thymelaeaceae) was found to show a potent antioxidant activity. Subsequent activity-guided fractionation of methanol extract of D. genkwa led to the isolation of four compounds from the ethyl acetate soluble fraction. The chemical structures were elucidated as genkwanin (1), 3'-hydroxygenkwanin (2), apigenin (3), and tiliroside (4) by spectroscopic techniques. Among them, compound 2 showed the significant anti-oxidative effect on DPPH. And compound 2 showed the significant riboflavin-and xanthine-originated superoxide quenching activities. To verify the antioxidant enzymatic activities of compound 2, the SOD enzymatic activity was measured spectrophtometrically using prepared Caenorhabditis elegans homogenates. The results showed that compound 2 was able to elevate SOD activity of C. elegans in a dose dependent manner. Moreover, compound 2 decreased the intracellular ROS accumulation of worms.

• 식물분류학회지
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• 제47권1호
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• pp.6-10
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• 2017

Microsatellite markers were isolated for Daphne kiusiana var. kiusiana (Thymelaeaceae), an evergreen broad-leaved shrub endemic to Korea and Japan. Because its populations in Jeju Island are morphologically controversial, and consistently threatened by anthropogenic pressures, taxonomic delimitation and conservation effort are required at the genetic level. We developed 21 polymorphic microsatellite loci from Next Generation Sequencing data. The primer set included di-, tri-, and tetra-nucleotide repeats. Variability in the markers was tested for 80 individuals of D. kiusiana from three natural populations in Jeju Island and Japan. Among the 21 loci, three were unavailable for population JKJU of Japan. The Neighbor-Joining tree based on microsatellite markers described here classified the three populations into two groups according to geographical or morphological traits. These will be a powerful genetics tool for determining the taxonomic boundary and establishing suitable conservation strategies for D. kiusiana in Jeju Island.

• 생물환경조절학회지
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• 제20권4호
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• pp.346-351
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• 2011

본 실험은 자생식물인 서향의 실내 분화로의 도입을 위해 대량 번식 및 광, 토양 조건 구명 실험을 수행하였다. 서향의 대량 번식을 위해 적합한 삽목 상토, 발근촉진제의 농도, 삽목 시기를 찾는 실험을 하였고, 실내로 도입하기 위해 인공광 처리(100, 1000, 2500lux)와 분화상토처리(원예용 혼합상토(바로커), 펄라이트 + 원예상토(1 : 1), 송이 + 원예상토(1 : 1), 마사토 + 원예상토(1 : 1), 모래 + 원예상토(1 : 1))를하였다. 삽목 상토 선발하기 위해 펄라이트, 버미큘라이트, 펄라이트와 버미큘라이트 혼용(1 : 1), 원예용상토 처리를 한 결과에서는 펄라이트와 버미큘라이트 혼용(1 : 1)처리 구에서 발근율이 93.3% 가장 우수하였다. 발근촉진제를 농도별로는 루톤과 IBA 100ppm 처리에서 뿌리 생육이 가장 양호하였다. 녹지삽 시기는 7월에 하는 것이 발근율이 96.7%로 우수하였다. 화분 이식 후 1000lux 이상의 광 조건에서 생육이 가장 좋았으며, 분화용 상토로는 제주 송이와 원예용 상토를 혼합한 토양에서 생육이 가장 좋았다.

• 생약학회지
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• 제35권1호통권136호
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• pp.62-79
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• 2004

Cell-scattering is a phenotypic change easily observed in most epithelial cells treated with Hepatocyte Growth Factor /scatter Factor (HGF/SF) or phorbol esters (PKC-activators). Recent studies have shown the possibilities to use as therapeutic materials of HGF/SF or non tumor promoting phorbol esters for liver disease, cancer and AIDS. In this study, we tested a cell-scattering activity of 534 methanol extracts from plants inhabiting in Korean peninsula using the phenotype-based assay system. Nine Active extracts were detected : Daphne genkwa, Daphne kiusiana, and Aleurites fordii showed high activity (+++), Euphorbia sieboldiana and Rhodotypos scandens showed medium activity (++), Sambucus sieboldiana var. pendula, Catalpa bignonioides, Sambucus sieboldiana and Lycoris squamigera showed low activity (+). Furthermore, the effects of these active materials in the culture cells were investigated with biochemical studies.

• Animal Systematics, Evolution and Diversity
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• 제40권2호
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• pp.150-156
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• 2024

A taxonomic review of the Korean Chrysis smaragdula group (Hymenoptera: Chrysididae), characterized by the six apical teeth (including lateral angles or obtuse protuberances) of metasomal tergum 3, is presented. Three species, namely Chrysis daphne, C. equestris, C. principalis, are included herein. Of these, C. equestreis is recorded from Korea for the first time. Chrysis daphne is a corrected name for the previous Korean records under the name of C. fasciata. A determination key to species complemented with illustrations is made. Additional diagnoses including color variations and descriptions of male genitalia (unavailable in C. equestris) based on Korean materials are also provided.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2002년도 총회 및 추계학술발표회
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• pp.110.1-110
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• 2002

• IMMUNE NETWORK
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• 제15권2호
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• pp.91-99
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• 2015

Herpes simplex virus (HSV) is a common causative agent of genital ulceration and can lead to subsequent neurological disease in some cases. Here, using a genital infection model, we tested the efficacy of vinegar-processed flos of Daphne genkwa (vp-genkwa) to modulate vaginal inflammation caused by HSV-1 infection. Our data revealed that treatment with optimal doses of vp-genkwa after, but not before, HSV-1 infection provided enhanced resistance against HSV-1 infection, as corroborated by reduced mortality and clinical signs. Consistent with these results, treatment with vp-genkwa after HSV-1 infection reduced viral replication in the vaginal tract. Furthermore, somewhat intriguingly, treatment of vp-genkwa after HSV-1 infection increased the frequency and absolute number of $CD3^-NK1.1^+NKp46^+$ natural killer (NK) cells producing interferon (IFN)-${\gamma}$ and granyzme B, which indicates that vp-genkwa treatment induces the activation of NK cells. Supportively, secreted IFN-${\gamma}$ was detected at an increased level in vaginal lavages of mice treated with vp-genkwa after HSV-1 infection. These results indicate that enhanced resistance to HSV-1 infection by treatment with vp-genkwa is associated with NK cell activation. Therefore, our data provide a valuable insight into the use of vp-genkwa to control clinical severity in HSV infection through NK cell activation.

• 한국환경복원기술학회지
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• 제20권3호
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• pp.33-44
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• 2017

This study investigated in the vascular plants of special protection zones (the native land of Berchemia berchemiaefolia in Sadam-ri and Gallon valley) of Songnisan National Park and identified the whole vascular plants. The numbers of vascular plants were summarized as 273 taxa including 82 families, 182 genera, 236 species, 3 subspecies, 28 varieties and 6 forms. Woody plants were identified as 124 taxa (45.4%) and herbaceous plants as 149 taxa (54.6%). A total of 107 taxa were identified in the area of Sadam-ri, and 246 taxa were found in the Gallon valley. A total of 8 taxa of rare plants were identified, each of which was divided into 1 taxa of endangered (EN; Daphne pseudomezereum var. koreana), 3 taxa of vulnerable (VU; Paeonia japonica, Berchemia berchemiaefolia and Iris minutiaurea), 3 taxa of least concern (LC; Aristolochia contorta, Syringa patula var. kamibayshii and Goodyera schlechtendaliana) and 1 taxa of data deficient (DD; Alangium platanifolium). The Korean endemic plants were 9 taxa (Clematis trichotoma, Thalictrum actaefolium var. brevistylum, Aster koraiensis, Cirsium setidens, etc.). In the specific plants by floristic region were 34 taxa, a degree I were 17 taxa (Chloranthus japonicus, Celastrus stephanotifolius, etc.), 9 taxa of a degree II (Euonymus pauciflorus, Acer triflorum, etc.), 4 taxa of a degree III (Aconitum longecassidatum, Angelica gigas, etc.), 2 taxa of a degree IV (Ulmus macrocarpa and Daphne pseudomezereum var. koreana), 2 taxa of a degree V (Berchemia berchemiaefolia and Alangium platanifolium). The naturalized plants were 5 taxa including Fallopia dumetorum, Trifolium repens, Ailanthus altissima, Oenothera biennis, Erigeron annuus. Naturalization rate (NR) was 1.8% of all 273 taxa of vascular plants and the invasive alien plants were not found. The purpose of this study is to identify the flora that grows in the special protection zones of Songnisan National Park and provide the basic data for the management of the special protection zone in the future.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.27-27
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• 2020

Daphne L. (Thymelaceae) comprises about 95 species distributing worldwide from N Africa, N India, SE Asia to E Asia and the coast of the Mediterranean of Europe. In Korea, five species of this genus have been described. In this study, we included four species (D. genkwa, D. pseudomezereum, D. kiusiana, D. jejudoensis) from Korea, excluding cultivated D. odora. The morphological characters through local surveys and the re-classification of the specimens collected in the Korea National Herbarium (KH) were carried out and distribution maps for each taxon were also prepared. The major characters include habit, trichomes in winter bud, leaf, and twig, phyllotaxis, inflorescence, size of calyx lobe and trichomes in the calyx tube, etc. The distribution map showed that D. genkwa is mainly distributed in the coastal area of Hwanghaenam-do, Pyeongannam-do, Jeollabuk-do and Jeollanam-do, whereas D. pseudomezereum is distributed in the limestone zone of Gangwon-do, Jeollabuk-do, and Gyeongsangbuk-do. Similarly, D. kiusiana is mostly found in Jeollanam-do, Gyeongsangnam-do, and Jeju-do. In addition, D. jejudoensis is known to be distributed in forests of Murueng, Andeok, and Seonheul-ri in Jeju-do, but recently, new habitat is discovered in the island forest areas of Jeollanam-do. However, some of these individuals showed the characteristics of D. kiusiana, thus before come to any conclusion detailed taxonomic review of D. jejudoensis and D. kiusiana is required.

• 한국환경과학회:학술대회논문집
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• 한국환경과학회 2018년도 정기학술대회 발표논문집
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• pp.214-214
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• 2018