• Korean Journal of Plant Taxonomy
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• v.43 no.2
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• pp.94-98
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• 2013

A new species, Daphne jejudoensis M. Kim, has been named and described from Seonheul, Jejudo Province, Korea. Daphne jejudoensis shares several characteristics (white calyx colors, long calyx tube, evergreen leaves, leathery leaf textures, etc.) with its related species D. kiusiana, but it is distinct from D. kiusiana which has a hairy calyx tube and lobes, short calyx lobes, oblanceolate leaves (with acute apex), and a different distribution (coastal region in Geojedo Is.) by having a glabrous calyx tube and lobes, long calyx lobes, elliptic leaves (with acuminate apex), and by its distribution (inland region in Jejudo Is.).

• Research in Plant Disease
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• v.22 no.1
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• pp.59-63
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• 2016

A new poty-like virus was isolated from plants of winter daphne (Daphne odora) that showed virus-like symptoms on leaves, from four regions of Korea during 2014. Filamentous-shaped particles were observed by transmission electron microscopy of preparations extracted from symptomatic leaves and examined by the direct negative stain method. RT-PCR assay showed that three samples were positive for both Cucumber mosaic virus and potyvirus, and only one sample was positive for potyvirus only. A BLAST comparison to partial sequences from helper-component proteinase, cylindrical inclusion and coat protein genes detected the highest nucleotide identity of 76%, 72%, and 72% with Daphne mosaic virus, respectively, levels below the potyvirus species discrimination threshold. The new potyvirus was isolated using indicator plants (Chenopodium amaranticolor), in which local lesions were produced. In this study, we identified a novel potyvirus from winter daphne, which we have named Daphne mottle virus (DapMoV).

• The Plant Pathology Journal
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• v.21 no.2
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• pp.102-105
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• 2005

Severe wilt disease epidemic was found on winter daphnes (Daphne odora Thunb.) cultivated in farmers, nurseries in Suncheon, Jeonnam in 2003. Typical symptoms appeared on the leaves of winter daphne as yellowish wilts and turned brown from the lower leaves on the same plant. Severely infected leaves were defoliated, resulting in blight of stems and eventual death of the entire plant. Black decayed vascular tissues were distinctly observed in a wilted plant. Fusarium sp. was isolated from the diseased plants repeatedly and its pathogenicity was confirmed by artificial inoculation on healthy plants. The fungus was identified as Fusarium oxysporum on the basis of the morphological and cultural characteristics on potato dextrose agar and carnation leaf agar. The optimum temperature for fungal growth was around $25{\circ}C$ and the fungal growth was inhibited by metconazole, triflumizole and trifloxystrobin on potato dextrose agar. This is the first report on the wilt disease of winter daphnes caused by F.oxysporum in Korea.

• Korean Journal of Plant Taxonomy
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• v.47 no.3
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• pp.207-221
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• 2017

Thymelaeaceae in Korea includes ten species in five genera (Daphne, Diarthron, Edgeworthia, Stellera, and Wikstroemia). This study investigated the morphology of these species using herbarium specimens and fresh materials obtained during fieldwork to understand the variation level and to delineate each taxon in the family. Our comparative morphological analysis showed that the Korean taxa in Thymelaeaceae were easily distinguished based on their morphologies. It is easy to distinguish Daphne from Wikstroemia when the Korean taxa are considered. However, Daphne genkwa is morphologically similar to Wikstroemia based on the leaf arrangement, pubescence of the leaves, and the ovary shape. The results here suggest that further systematic studies of the Daphne group using more data are necessary.

• Korean Journal of Plant Taxonomy
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• v.51 no.2
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• pp.171-175
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• 2021

Daphne genkwa (Thymelaeaceae) is a small deciduous shrub widely cultivated as an ornamental. The complete chloroplast genome of this species is presented here. The genome is 132,741 bp long and has four subregions: 85,668 bp of large single-copy and 28,365 bp of small single-copy regions are separated by 9,354 bp of inverted repeat regions with 107 genes (71 protein-coding genes, four rRNAs, and 31 tRNAs) and one pseudogene. The phylogenetic tree shows that D. genkwa is nested within Wikstroemia and is not closely related to other species of Daphne, suggesting that it should be recognized as a species of Wikstroemia.

• Journal of Forest and Environmental Science
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• v.35 no.1
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• pp.31-40
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• 2019

Daphne pseudomezereum var. koreana is an endangered deciduous shrub distributed in mountain areas that is vulnerable to climate change. The purpose of this study was to provide foundational data on the physical characteristics, soil environment, and vegetation structure of habitats of Daphne pseudomezereum var. koreana habitat in Korea in order to help with management decisions on ecosystem restoration. Rock exposure was 15 to 35%, with an average of 24%. The native habitat of D. pseudomezereum included 129 taxa consisting of 46 families and 95 genera. Two-way cluster analysis divided the habitat into three plant communities: Community I (dominaterd by Tilia amurensis and Quercus mongolica), Community II (dominaterd by Fraxinus rhynchophylla and Acer pseudosieboldianum), and Community III (dominaterd by Ulmus davidiana var. japonica). The diversity indices for Communities I, II, and III were 1.124, 1.047 and 0.932, respectively. The soils were loam or clay loam. Soil pH, organic matter content, and available phosphoric acid were 5.40, 14.38%, and 31.08 ppm, respectively. Ordination analysis resulted that most significant factors influencing D. pseudomezereum distribution were magnesium content of soil, shrub layer, and altitude.

• Journal of Korean Society of Forest Science
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• v.109 no.2
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• pp.189-194
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• 2020

Daphne pseudomezereum var. koreana is native to Korea and is distributedin Kangwon-do, Jeollabuk do, and Gyeongsang-do. This economically valuable species has experienced a dramatic decrease in natural habitat due to climate change and is difficult to cultivate. In this study, we investigate a mass propagation method for D. pseudomezereum through in vitro culture and genetic resource preservation.WPM medium was better than the MS medium for shoot growth. As a result, we compared the shoot number and length of apical (W/AP) and non-apical shoots (W0/AP) with BA and GA₃ treatments in WPM medium. Their shoots and length grew well in both BA 8ìM + GA₃8ìM-treated apical shoot and without-apical shoot. NAA did not effectively induce rooting of the in vitro plantlet.

• Journal of Bio-Environment Control
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• v.19 no.4
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• pp.246-250
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• 2010

This study was conducted to find out the propagation method of a rare variety Daphne kiusiana Miq. in Korea through softwood cuttings. Daphne kiusiana Miq are the evergreen broad leaf tree which have lived in the southern part of Korea. The flowering time of Daphne kiusiana Miq is early spring and the flower has an attractive fragrance. Generally, plants are dioecious and only the male plants are found in Korea. Propagation by seed is very difficult in this tree. Softwood cuttings were taken to establish an efficient propagation method in Daphne kiusiana Miq Cuttings were grown in perlite:vermiculite (1 : 1), perlite, vermiculite, sand rooting media under shaded greenhouses during summer in 2007. The rooting rates of cuttings were 86.7%, 95%, 75% and 95% in perlite:vermiculite (1 : 1), perlite, vermiculite and sand media, respectively. Softwood cuttings taken on July and rooting at $27^{\circ}C$ were significantly improved the growth and rooting rates. Softwood cutting can be an effective means of propagation in Daphne kiusiana Miq.

• Bulletin of the Korean Chemical Society
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• v.35 no.2
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• pp.669-671
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• 2014

• Research in Plant Disease
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• v.11 no.1
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• pp.72-76
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• 2005

In 2002 and 2003, Phytophthora blight on Daphne odora occurred in fields at Waryong Mountain, Sacheon city, Korea. The disease usually initiated on the root and crown of the plant. Crown lesions began with light brown as water-soaking. Infection rate of the disease reached up to 60% in a heavily infested fields. The causal fungus was identified as Phytophthora nicotianae based on following mycological characteristics. Sporangium: readily formed in water, papillate, noncaducous, ovoid to spherical, 23~$56{\times}$~34 $\mu\textrm{m}$ in size. Oogonium: spherical, smooth walled, 18~26 $\mu\textrm{m}$ in size. Oospore: aplerotic, globose, 16~24 $\mu\textrm{m}$ in size. Antheridium: amphigynous, unicellula, spherical. Chlamydospore: abundant, spherical, 16~32 $\mu\textrm{m}$ in size. Sexuality: heterothallic. Optimum growth temperature was about 25~$30^{\circ}C$. Pathogenicity of the isolate was confirmed on host plant and the fungus was identified as Phytophthora nicotianae. This is the first report of Phytophthora blight of Daphne odora in Korea.