• Journal of Microbiology and Biotechnology
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• v.20 no.10
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• pp.1367-1377
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• 2010

Scientific research regarding the use of live bacterial cells for therapeutic purposes has been rapidly growing over the years and has generated considerable interest to scientists and health professionals. Probiotics are defined as essential live microorganisms that, when administered in adequate amounts, confer a health benefit on the host. Owing to their considerable beneficial health effects, these microorganisms are increasingly incorporated into dairy products; however, many reports have demonstrated their poor survival and stability. Their survival in the gastrointestinal tract is also questionable. To overcome these problems, microencapsulation techniques are currently receiving considerable attention. This review describes the importance of live probiotic bacterial microencapsulation using an alginate microparticulate system and presents the potentiality of various coating polymers such as chitosan and polylysine for improving the stability of this microencapsulation.

• Annual Conference of KIPS
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• 2014.11a
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• pp.2-5
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• 2014

국내 낙농가는 일 년 내내 과중한 업무에 시달리지만 낙농 효율이 바닥으로 떨어지고 있다. 낙농 효율을 높이기 위해 구축 되어진 낙농 솔루션들이 있지만 각 솔루션별로의 수집되는 많은 데이터를 바탕으로 사람이 종합적인 판단을 수행하고 이를 낙농경영에 적용시키기에는 많은 무리가 따른다. 본 연구에서는 이미 구축되어진 낙농 솔루션을 통하여 수집되는 데이터를 실시간 분석 및 예측을 수행함으로써 낙농가가 보다 효율적인 개체관리(건강, 착유, 번식, 급이), 경영관리, 환경관리를 수행하고 낙농가의 고된 업무를 자동화 시스템을 통하여 지원 할 수 있는 시스템 설계를 제안한다.

• Food Science and Industry
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• v.52 no.4
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• pp.387-400
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• 2019

Fat replacers are divided into three categories. These include carbohydrate-based, protein-based and fat-based replacers. Carbohydrate-based replacers occupy half of the fat replacers market. The main ingredients of carbohydrate-based are gums, starch, modified starch, cellulose and fiber. The functional properties of fat replacers are to retain moisture, to retard staling, to provide mouthfeel and texture, to emulsify, to stabilize emulsion, and to reduce fat. Using these functionalities, fat replacers are used in various foods such as baked goods, salad dressing, sauces, meat products, dairy products, frying foods, bakery, and confectionery. Success factors of fat replacers in the market are sensory equivalent, texture and safety as food ingredients.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2001.06a
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• pp.155-157
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• 2001

Bifidobacterium spp. is nonpathogenic, gram-positive and anaerobic bacteria, which inhabit the intestinal tract of humans and animals. In breast-fed infants, bifidobacteria comprise morethan 90％ of the gut bacterial population. Bifidobacteria spp. are used in commericial fermented dairy products and have been suggested to exert health promoting effects on the host by maintaining intestinal microflora balances, improving lactose tolerance, reducing serum cholesterol levels, increasing synthesis of vitamins, and aiding the immune enchancement and anticarcinogenic activity for the host. These beneficial effects of Bifidobacterium are strain-related. Therefore continued efforts to improve strain characteristics are warranted. in these respect, development of vector system for Bifidobacterium is very important not only for the strain improvement but also because Bifidobacterium is most promising in serving as a delivery system for the useful gene products, such as vaccine or anticarcinogenic polypeptides, into human intestinal tract. For developing vector system, we have characterized several bifidobacterial plasmids at genetic level and developed several shuttle vectors between E. coli and Bifidobacterium using them. Also, we have cloned and sequenced several metabolic genes and food grade selection marker. Also we have obtained bifidobacterial surface protein, which will be used as the mediator for surface display of foreign genes. Recently we have succeeded in expressing amylase and GFP in Bifidobacterium using our own expression vector system. Now we are in a very exciting stage for the molecular breeding and safe delivery system using probiotic Bifidobacterium strains.

• Journal of Software Assessment and Valuation
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• v.17 no.2
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• pp.117-124
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• 2021

Livestock behavioral analysis is a factor that has a great influence on livestock health management and agricultural productivity increase. However, most digital devices introduced for behavioral analysis of livestock do not provide raw data and also provide limited analysis results. Such a closed system makes it more difficult to integrate data and build big data, which are essential for the introduction of advanced IT technologies. Therefore, it is necessary to supply farm-scale data collection devices that can be easily used at low cost. This study presents a data collection system for analyzing the behavior of livestock. The system consists of a number of miniature computing units that operate wirelessly, and collects livestock body temperature and acceleration data, location information, and livestock environment data. In addition, this study presents an algorithm for estimating the behavior of livestock based on the collected acceleration data. For the experiment, a system was built in a Korean cattle farm in Icheon, Gyeonggi-do, and data were collected for 20 Korean cattle, and based on this, the empirical and analysis results were presented.

• Food Science of Animal Resources
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• v.43 no.2
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• pp.220-231
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• 2023

Although omega-3 fatty acids including docosahexaenoic acid (DHA) contain various health-promoting effects, their poor aqueous solubility and stability make them difficult to be induced in dairy foods. The aims of this research were to manufacture casein derivative-based delivery system using acid-induced gelation method with glucono-σ-lactone and to investigate the effects of production variables, such as pH and charged amount of linoleic acid, on the physicochemical properties of delivery systems and oxidative stability of DHA during storage in model milk. Covalent modification with linoleic acid resulted in the production of casein derivatives with varying degrees of modification. As pH was reduced from 5.0 to 4.8 and the charged amount of linoleic acid was increased from 0% to 30%, an increase in particle size of casein derivative-based delivery systems was observed. The encapsulation efficiency of DHA was increased with decreased pH and increased charged amount of linoleic acid. The use of delivery system for DHA resulted in a decrease in the development of primary and secondary oxidation products. An increase in the degree of modification of casein derivatives with linoleic acid resulted in a decrease in the formation of primary and secondary oxidation products than of free DHA indicating that delivery systems could enhance the oxidative stability of DHA during storage in model milk. In conclusions, casein derivatives can be an effective delivery system for DHA and charged amount of linoleic acid played a key role determining the physicochemical characteristics of delivery system and oxidative stability of DHA.

• Journal of the East Asian Society of Dietary Life
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• v.23 no.6
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• pp.671-680
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• 2013

This study was conducted to examine the consumption realities of processed food and the perception of food labeling with targets of university students attending schools in Daegu and Gyeongbuk areas. The reason for buying processed food was because it is easy to cook. As for the number of using processed food, it was answered that everyday dairy products, noodles, breads and snacks for 3~4 times a month, and meat processed foods, canned foods, sauces and frozen foods for 2~3 times a month. With regard to selecting processed products, both male and female students answered with taste and price first, barely considering the safety and nutritional elements. It turned out that they check the price, expiration date and country of origin in order upon buying processed foods. Most of them recognized food labeling, but answered that they checked it sometimes in order to check the 'expiration date' and the 'calories'. They were aware of the food nutritional contents indication. It was turned out that male students check it for nutrients and female students for weight control. The effect of the processed food indication was that it is easy to choose the necessary foods for health and as well as for comparing the food with other products. The satisfaction of the food labeling system was mediocre. The results indicated that the food indication system needs to be educated or promoted.

• Journal of Microbiology and Biotechnology
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• v.20 no.1
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• pp.179-186
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• 2010

In biological wastewater treatment, high lipid concentrations can inhibit the activity of microorganisms critical to the treatment process and cause undesirable biomass flotation. To reduce the inhibitory effects of high lipid concentrations, a two-phase anaerobic system, consisting of an anaerobic sequencing batch reactor (ASBR) and an upflow anaerobic sludge blanket (UASB) reactor in series, was applied to synthetic dairy wastewater treatment. During 153 days of operation, the two-phase system showed stable performance in lipid degradation. In the ASBR, a 13% lipid removal efficiency and 10% double-bond removal efficiency were maintained. In the UASB, the chemical oxygen demand (COD), lipid, and volatile fatty acid (VFA) removal efficiencies were greater than 80%, 70%, and 95%, respectively, up to an organic loading rate of 6.5 g COD/l/day. No serious operational problems, such as significant scum formation or sludge washout, were observed. Protein degradation was found to occur prior to degradation during acidogenesis.

• Journal of Biosystems Engineering
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• v.34 no.4
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• pp.228-233
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• 2009

In order to cut the round bale easily, a roughage cut-feeder for the round bale was developed in this study. This study consists of two parts. One is development of a cutting mechanism and a performance test reported in the previous paper. This is the second part of the study. For the study, recent trend of traditional cutting system was analyzed. Also, cost of the prototype cut-feeder was analyzed and determine the break-even point of farm size was determined by comparing with traditional method using a traditional cutter. A prototype cut-feeder was investigated with the fact that working performance, fiber length and shape of roughage and feeding quality was good. Operating cost of the prototype cut-feeder decreased rapidly with an increase of farm size. Break-even point in terms of farm size was 36 heads for beef and 28 heads for dairy. Also, costs estimated were 118,000 won/head year and 148,000 won/head year, respectively.

• Food Science of Animal Resources
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• v.39 no.1
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• pp.13-22
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• 2019

This study aimed to develop a bile-responsive expression system for lactobacilli. The promoters of four genes, encoding phosphoenolpyruvate-dependent sugar phosphotransferase (mannose-specific), L-lactate dehydrogenase (LDH), HPr kinase, and D-alanine-D-alanine ligase, respectively, which were highly expressed by bile addition in Lactobacillus johnsonii PF01, were chosen. Each promoter was amplified by polymerase chain reaction and fused upstream of the ${\beta}$-glucuronidase gene as a reporter, respectively. Then, these constructs were cloned into E. coli-Lactobacillus shuttle vector pULP2, which was generated by the fusion of pUC19 with the L. plantarum plasmid pLP27. Finally, the constructed vectors were introduced into L. plantarum for a promoter activity assay. The LDH promoter showed the highest activity and its activity increased 1.8-fold by bile addition. The constructed vector maintained in L. plantarum until 80 generations without selection pressure. A bile-responsive expression vector, $pULP3-P_{LDH}$, for Lactobacillus spp. can be an effective tool for the bile-inducible expression of bioactive proteins in intestine after intake in the form of fermented dairy foods.