• Asian-Australasian Journal of Animal Sciences
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• 제13권1호
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• pp.31-38
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• 2000

This study was conducted to evaluate changes in the lysine to digestible energy (DE) ratio on performance, apparent ileal and fecal nutrient digestibilities as well as blood urea nitrogen (BUN), and to estimate optimal lysine:DE ratios for growing pigs of different sexes. A total of 150 pigs ($(Landrace{\times}Yorkshire){\times}Duroc$, 16.78 kg average body weight, 75 barrows and 75 gilts) was randomly allotted into a $2{\times}3$ (sex by diet) factorial design. Three diets were formulated to contain a crude protein level of 19%, a DE level of 3.5 Mcal/kg with three lysine:DE ratios of 3.2 (low), 3.5 (middle) and 3.8 (high) g lysine/Mcal DE per kg diet for both barrows and gilts throughout the study. With increasing dietary lysine:DE ratio, the average daily gain (ADG) of barrows decreased but there was no significant difference among treatments (p>0.05). However, ADG was significantly higher in gilts fed the diet containing the high lysine:DE ratio (p<0.05), followed by the middle and low lysine:DE ratio dietary groups. No significant effects of lysine:DE ratios on feed intake (ADFI) and feed conversion (F/G) were observed for barrows and gilts during overall period (p>0.05), while the optimal F/G was found in barrows fed diets of low and in gilts fed high lysine:DE ratio. Blood urea nitrogen had a positive relationship with growth rate. The results showed that the optimal lysine:DE ratios were 3.2 and 3.8 g lysine/Mcal DE per kg diet for barrows and gilts of 16 to 57 kg body weight, respectively.

• Asian-Australasian Journal of Animal Sciences
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• 제27권4호
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• pp.551-560
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• 2014

Twenty-four male 1-year old swamp buffaloes (Bubalus bubalis) were randomly allocated to 4 groups. One group grazed on guinea grass (GG) and another on guinea grass and the legume Stylosanthes guianensis (GL). The other two groups were kept in pens and fed freshly cut guinea grass and concentrate at an amount of 1.5% (GC1.5) and 2.0% (GC2.0) of body weight, respectively. The effect of the different feeding intensities on carcass characteristics and meat quality were assessed. The mean body weight at slaughter was 398 (${\pm}16$) kg. Average daily gain was higher in concentrate-supplemented groups (570 and 540 g/d in GC1.5 and GC2.0, respectively) when compared to GG (316 g/d) and GL (354 g/d) (p<0.01). Likewise, the warm carcass weight was higher in GC1.5 and GC2.0 compared to GG and GL. Dressing percentage was 48.1% and 49.5% in GC1.5 and GC2.0 in comparison to 42.9% and 44.8% observed in GG and GL, respectively. Meat of Longissimus throracis from GC1.5 and GC2.0 was redder in color (p<0.01), while water holding capacity (drip and thawing loss) was improved in pasture-fed groups (p<0.05). Protein and fat content of Longissimus thoracis was higher in animals supplemented with concentrate (p<0.01), as was cholesterol content (p<0.05), whereas PUFA:SFA ratio was higher and n-6/n-3 ratio lower (p<0.01) in pasture-fed buffaloes. Results of the present study showed that the supplementation of pasture with concentrate enhances the growth and carcass characteristics of swamp buffaloes expressed in superior dressing percentage, better muscling, and redder meat with a higher content of protein and fat, whereas animals grazing only on pasture had a more favorable fatty acid profile and water holding capacity. In conclusion, the supplementation of concentrate at a rate of about 1.5% of body weight is recommended to improve the performance and carcass quality of buffaloes.

• Asian Pacific Journal of Cancer Prevention
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• 제13권9호
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• pp.4597-4606
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• 2012

Cholangiocarcinoma (CCA) is an uncommon adenocarcinoma which arises from the epithelial cells of the bile ducts. The aim of the study was to investigate the cytotoxicity, toxicity, and anticancer activity of a crude ethanolic extract of ginger (Zingiber officinale Roscoe) against CCA. Cytotoxic activity against a CCA cell line (CL-6) was assessed by calcein-AM and Hoechst 33342 assays and anti-oxidant activity was evaluated using the DPPH assay. Investigation of apoptotic activity was performed by DNA fragmentation assay and induction of genes that may be involved in the resistance of CCA to anticancer drugs (MDR1, MRP1, MRP2, and MRP3) was examined by real-time PCR. To investigate anti-CCA activity in vivo, a total of 80 OV and nitrosamine (OV/DMN)-induced CCA hamsters were fed with the ginger extract at doses of 1000, 3000, and 5000 mg/kg body weight daily or every alternate day for 30 days. Control groups consisting of 10 hamsters for each group were fed with 5-fluorouracil (positive control) or distilled water (untreated control). Median $IC_{50}$ (concentration that inhibits cell growth by 50%) values for cytotoxicity and anti-oxidant activities of the crude ethanolic extract of ginger were 10.95, 53.15, and $27.86{\mu}g/ml$, respectively. More than ten DNA fragments were visualized and up to 7-9 fold up-regulation of MDR1 and MRP3 genes was observed following exposure to the ethanolic extract of ginger. Acute and subacute toxicity tests indicated absence of any significant toxicity at the maximum dose of 5,000 mg/kg body weight given by intragastric gavage. The survival time and survival rate of the CCA-bearing hamsters were significantly prolonged compared to the control group (median of 54 vs 17 weeks). Results from these in vitro and in vivo studies thus indicate promising anticancer activity of the crude ethanolic extract of ginger against CCA with the absence of any significant toxicity. Moreover, MDR1 and MRP3 may be involved in conferring resistance of CCA to the ginger extract.

• Asian-Australasian Journal of Animal Sciences
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• 제33권6호
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• pp.921-929
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• 2020

Objective: The aim of the present study was to investigate the beneficial effects of dietary supplementation with oil palm frond (leaf) (OPF) with and without oil palm meal (OPM) on nutrient intake and digestibility, ruminal fermentation and growth performance in goats. Methods: Six female crossbred goats were fed for 28 days of 3 diet treatments; 100% para-grass (T1); 50% para-grass + 50% OPF (T2), and 30% para-grass + 50% OPF + 20% OPM (T3). Body weight, rectal temperature, respiratory rate, and urine volume, food intake, dry matter intake and water intake were measured daily. Nutrient digestibility was determined from five consecutive days of last week in each diet. Ruminal fluid, urine and blood were collected at the end for determination of rumen protozoa and volatile fatty acid contents, urinary allantoin excretion, blood cell count and chemistry profiles. Results: Goats fed T2 and T3 showed higher dry matter and nutrients intakes while protein digestibility was suppressed compared with those for T1. Crude fat digestibility declined in T2 but maintained after adding the OPM (T3). High fat intake by giving OPF and OPM corresponded to a higher ruminal acetate/propionate ratio (C2/C3) and serum cholesterol level. An increased urinary allantoin/creatinine ratio was found in T2 and T3 compared with T1, implying an increased number of ruminal microbes. Conclusion: Increased dry matter intake in T2 and T3 suggested that oil palm by-products are partly useful as a replacement for para-grass in goats. Replacement with the by-products increased plasma cholesterol level, which suggested that these products are a useful energy source. Changes in rumen parameters suggested an increased microbial number and activity suitable for acetate production. However, the limited digestibility of protein implies that addition of high protein feeds may be recommended to increase body weight gain of goats.

• 한국언론정보학보
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• 제67권
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• pp.88-118
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• 2014

이 연구는 첫째, 현대 한국사회의 신문사에 의해 발화되는 '민생'이 어떤 의미를 가지며, 그것을 지칭하는 구체적인 대상이 무엇인가를 밝히고자 했다. 둘째, 신문사에 의해서 형성되는 거대담론과 시간의 흐름에 따라 변하는 것 그리고 변하지 않는 것이 무엇인지를 고찰하고자 했다. 이를 위해 한국의 주요 종합일간지의 표제어에 '민생'이란 키워드를 담고 있는 사설들을 분석했다. 이를 통해 민생담론은 누적적으로 형성된 담론 관행이 시간의 흐름과 사회변화에 따라 변하는 것을 알 수 있었다. 정치적 관점에서는 정부별로 담론이 형성되고 전환되는 양상이 뚜렷하게 나타났다. 민생을 대변한다는 암묵적인 전제하에, 정부 정치권과 저항세력에 대해 적대적이나 공격적인 진술을 정당화했다. 또한 민생담론은 한국의 민주화 세계화 과정이라는 거대한 사회적 흐름과 관계했고, 민주화의 발전과정과 함께 비판의 주된 대상이 정부중심에서 정당중심으로 변해온 것을 확인할 수 있었다. 경제적인 관점에서 민생담론이 노동쟁의, 물가, 통화증가율, 주택문제, 외환위기 등을 해결하기 위한 노력이었다면, 환란이후인 2000년대 들어서는 경제성장과 분배문제부터 양극화 문제, 일자리 창출, 비정규직 철폐 등의 문제들로 전환되는 양상을 보였다. 우리사회에 위기가 닥칠 때마다 민생경제 담론은 경제정책을 주도하는 주체에 대해 비판을 가했지만, 위기를 벗어나려는 대안의 모색이나 구체적인 분석을 제시한 것은 아니었다. 결론적으로 한국의 주요종합일간지 신문사설에 의해서 생산되는 '민생'이란 단어의 의미는, 사전적 정의로서의 '국민생활' 그 자체와 거리가 있었다.

• 생물환경조절학회지
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• 제8권3호
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• pp.192-201
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• 1999

본 연구는 ASKAM모델로 $CO_2$, 광 및 엽면적지수가 단고추의 동적 광합성율에 추정가능성을 평가하기 위하여 수행되었다 $CO_2$ 농도는 2구획으로 구분된 반밀폐된 생장상에서 3수준 (400, 700 or 1000$\mu$mo1 mol$^{-1}$)으로 조절하였다. 온도와 습도는 21$^{\circ}C$ 및 80-95%로 조절하였고, 양액이 순환되는 용기내에서 재배하였다. 일태양복사, 온도 및 $CO_2$농도의 단기 자료를 기초로 하여 일중 단기간격에 대한 작물 순$CO_2$흡수량를 ASKAM모델로 추정하였고, 광합성 측정 중 각 구획과 기부에서의 $CO_2$농도를 매분마다 측정하였다. 순수 $CO_2$의 구획으로의 흐름, 지상방사, 구획내의 광합성유효방사, 은도 및 상대습도를 측정하였다. 본 작물생장모델은 작물의 광합성율을 적절하게 추정할 수 있는 것으로 평가되므로 생장해석, 작물관리 및 온실관리에 광범위하게 적용될 수 있을 것으로 판단되었다.

• 한국양식학회지
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• 제19권4호
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• pp.231-235
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• 2006

본 연구는 항암, 항균, 항산화 활성이 뛰어나다고 보고되고 있는 버섯균사체를 사료첨가제 이용 가능성을 확인하기 위하여 실험을 실시하였다. 일반 시중에서 판매중인 배합사료에 상황버섯 균사체와 운지버섯 균사체를 혼합배양한 배양액 1개와, 상황버섯 균사체와 운지버섯균사체를 각각 단독으로 배양한 배양액 2개로 총 3종류의 배양액을 가지고 배합사료와 혼합하여 12주 동안 넙치에게 급이 하여 성장률, GPT측정 및 V. anguillarum을 이용하여 공격 실험한 결과 대조구에 비해 균사체배양액이 첨가된 실험구에서 좋은 효과를 보였다. 성장률인 경우 대조구에 비해 실험구가 $12{\sim}20%$ 가량 어체중이 증가와 어체의 평균 전장이 길이도 전체적으로 증가하는 양상을 나타내었다. 또한 10주에서는 운지버섯(CV)과 상황버섯(PL) 균사체 혼합 배양액을 첨가한 사료의 실험구가 다른 실험구에 비해 높은 체중을 나타냈다. 넙치의 간수치를 실험한 결과 GPT 측정 Karamen unit 수치가 사육기간이 지날수록 지속적으로 낮아졌으며, 실험구들 또한 대조구와 비교해보았을 때 전체적으로 수치가 낮은 것을 확인할 수가 있었다. 인위적인 세균 공격실험의 경우 전체적으로 실험구가 대조구에 비해 $20{\sim}40%$ 정도의 높은 생존율의 차이를 보였으며, 특히 혼합배양액을 첨가한 실험구의 경우 가장 높은 40%의 생존율을 나타냈다. 그러므로 버섯 균사체 배양액을 일반 사료에 사료첨가제로서의 이용가능성이 매우 크다고 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제22권8호
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• pp.1140-1150
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• 2009

Forage diets provide good quality carcasses in sheep but very little is known in tropical goats. An experiment was designed with Creole male goats using grass-based systems to assess carcass yield, scores, cuts and composition. After weaning (84 d, 9.2 kg LW) two modes of forage feeding were compared with two replicates of each. Feeding groups were: PF for animals reared at pasture (n = 62) and IF when reared indoors (n = 60). Given that forage finishing will result in low ADG it appeared necessary to study different fattening lengths. The kids were equally divided into 4 groups: group A (n = 32), 4mo after weaning; group B (n = 32), 4mo after A; group C (n = 30), 3mo after B and group D (n = 28), 2mo after C. The animals grazed (in two sub-flocks) on irrigated tropical pastures managed in a rotational system (28 d of re-growth) at a mean stocking rate of 1,200 kg/ha/yr LW. The IF groups were reared in collective pens on a slatted floor (2 replicates of 7 or 8 kids each). They were fed the same stand of tropical grass (25% DM, 12% CP) as that of pasture that was cut daily and provided ad libitum. The ADG (-10%), the weights of omental fat (-60%) and fat in shoulder (-18%), the ultimate pH of carcass (-12%), the meat colour score (-24%), the ""parameter accounting for redness (12%) and the DM and lipid contents (-4%) were significantly lower (p<0.05) in PF than in IF, while the liver was heavier (+23%, p<0.05). Feeding conditions seemed to be similar, thus, differences could be related to gastrointestinal parasitism in the PF system and hypotheses are discussed. Increasing the fattening duration, resulted in significant difference (p<0.01) in many traits: the weights at slaughter and of carcass increased by 40% and 60% from groups A to D and consequently the weights of body compartments and carcass cuts (1.5 to 2.0 fold more). When the results were presented as percentage of empty body weight and carcass weight, these preliminary results (carcass weight 9kg and yield 53%, muscle proportion 70%) and qualitative parameters (low fat score 2/5, fat proportion 5%), seem to be a good incentive for the sector to develop a niche market to meet consumer lean meat expectations. The indoors system could be implemented where there was low availability of grazing areas or problems of dog attacks.

• Asian-Australasian Journal of Animal Sciences
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• 제15권10호
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• pp.1428-1432
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• 2002

Thirty-six (20 wethers and 16 rams) adult Gansu highland finewool sheep with an average liveweight of $19.42{\pm}1.83kg$ were used. The sheep were allocated to 4 treatments and confined in individual feeding stalls. The 80 day feeding trial was divided into 2 periods of 38 and 42 days. The sheep had access to clean water and were fed chopped wheat straw ad libitum. Daily supplemented diets of 150 g for the first period and 200 g for the later period were provided. The 4 types of supplements were: Wheat bran type (I), which is the traditional feeding practice in the area and the control group; Linseed cake type (II); Linseed cake+poultry litter type (III); Poultry litter+distiller grains type (IV). The digestibility trial was conducted using a total collection method at the end of the first period in the feeding trial. The results showed that the digestibilities of DM, OM, CP, CF, NDF and ADF in group II and III were increased to more than 50%. CP and NDF digestibility in group II were significantly increased by 23.6% and 25.5% respectively over group I (p<0.01) and by 10.1% and 13.1% respectively over group III (p<0.05). The digestibility of DM, OM, CP, CF, NDF and ADF in group IV was lower than group I by 4.4 to 8.4% (p>0.05). Compared to group I, group II and III increased straw intake by 17.6% (p<0.01) and 10.8% (p<0.05). The ADG increased from 16.2 g in group I to 45.3 g and 32.8 g (p<0.01) in group II and III respectively. The ratio of supplement intake to gain decreased from 10.7 in group I to 3.9 and 5.4 (p<0.01) in group II and III respectively. The ratio of supplement cost to gain also decreased by 60.0% and 64.3%, respectively. The wool growth rate and wool strength in these 2 groups were noticeably improved. This significantly enhanced the wool's textile value. The above parameters in group IV were lower than in group I, except the ratio of supplement cost to gain. This research indicates that local low quality nitrogen resources could be used effectively and economically to improve the utilization of wheat straw fed to sheep on smallholder farms under harsh loess plateau conditions in the winter.

• Asian-Australasian Journal of Animal Sciences
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• 제13권12호
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• pp.1659-1666
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• 2000

Two experiments were conducted to determine the effects of Leucaena leucocephala (leucaena) and Gliricidia sepium (gliricidia) supplementation on intake, digestion, outflow rates, microbial protein yield and live-weight changes in sheep fed with ammoniated rice straw (ARS). In experiment 1, three rumen cannulated Japanese Corriedale wether (mean body weight of 35.6 kg) in $3{\times}3$ Latin Square Design were used. Animals were fed ad libitum ARS alone, or supplemented with 200 g of either leucaena or gliricidia. In experiment 2, twenty-four growing native Philippine sheep with average body weight of $13.5{\pm}0.25kg$ were used in a completely randomized design (CRD) and offered similar diets to those of experiment 1. Supplementation increased total dry matter intake and nutrient digestibility except for fiber (p<0.05) without affecting ARS consumption. Nitrogen balance revealed that absorbed and retained N was significantly higher in leucaena and gliricidia. The significant improvement in N utilization and more digestible OM intake brought about by the inclusion of leucaena and gliricidia to ARS resulted in increased (p<0.05) microbial N yield. Efficiency of microbial N supply in supplemented group was not significantly different, but higher (p<0.05) than the 24.92 g N/kg DOMR for ARS group. Liquid outflow rate was 7.8 and 6.8 %/h, while the solid phase of rumen digesta was 4.4 and 3.8 %/h for the leucaena and gliricidia group respectively, which were significantly higher than 5.30 and 2.50 %/h in the control diet. The increase in total DMI resulted to higher (p<0.01) growth performance and efficient feed utilization. Average daily gain (ADG) was 19.3, 34.6 and 33.9 g/d for the ARS, leucaena and gliricidia respectively. It is therefore concluded that addition of leucaena and gliricidia to ARS in could increase nutrient intake and digestibility, subsequently improving N utilization and livestock performance.