• Journal of The Korean Society of Grassland and Forage Science
• /
• v.20 no.2
• /
• pp.103-108
• /
• 2000

To produce of transgenic orchardgrass, the seed-derived calli of orchardgrass (Dactylis glomerata L.) co-cultivated with Agrobacterium turnefaciens EHAlOl harboring binary vector pIG121-Hm were selected with hygromycin and then transferred onto N6 regeneration medium containing 1 rngl l of NAA, 5 rngl l of kinetin, 250 rngl l of carbenicillin and 50 mg/ l of hygromycin. The efficiency of transformation was differed on cultivars, that is, 'Potomac' appeared 12% of transformation efficiency while 'Amba' did 5.5%. The addition of acetosyringone during co-cultivation was a key to successhl transformation of orchardgrass. Transgene fragments were identified by PCR analysis and the constitutive expression of GUS gene was confirmed by Northern blot analysis. (Key words : Acetosyringone, Agrobacterium tumefaciens, Orchardgrass (Dactylis glomerata L.), Transformation)

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.13 no.3
• /
• pp.184-189
• /
• 1993

Orchardgrass(Dactylis glomerata L.) hays, which had been harvested in different seasons and grown with different levels of potassium (K)fertilizer, were fed to foats in metabolic cages in order to know the effect forage mineral content on mineral balance of goats. The K contents of the feed were 3.4, 4.9 and 5.8% and magnesium (Mg) contents were 0.26, 0.21 and 0.21% on a det matter(DM) basis in K-low, K0medium and K-high treatments, respectively. Urinary K excretion was significantly more on K-high than on K-low treatment. Faecal and urinary Mg excrerions on K-low were higher than those on K-medium and K-high feeds. And there was a tendency of positive relation between urinary Mg excretion and serum Mg concentrarion. Goats on K-high treatment seemed to drink more water and excrete more urine than those on the other feeds.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.24 no.1
• /
• pp.25-28
• /
• 2004

To determine lethal temperature of orchardgrass (Dactylis glomerata L. cv. Janbeol 102) developed in Korea at heat-stressed conditions, seedlings grown in a amall pots for 4 weeks were treated at $45^{\circ}C$, $50^{\circ}C$ or $55^{\circ}C$ for 1 h. Heat treatments at $60^{\circ}C$ and $65^{\circ}C$ for 1 h, several plants were withered and showed damage symptom on their leaves. When the plants were exposed to $70^{\circ}C$ for 1 h, most of leaves were severely withered, but it was not lethal conditions for the whole plants. By contrast, most of plants were died within one day after heat treatment at $80^{\circ}C$ for 1h. Furthermore, plants exposed to $80^{\circ}C$ for 55 min were also died within 7 days. It was found that new shoots were regenerated from the plants that had been treated at $80^{\circ}C$ within 50 min. These results indicate that heat treatment at $80^{\circ}C$ for 55 min is an optimum condition to distinguish the lethality of orchardgrass plants. Simple viability assay system established in this study will be useful for selection and characterization of heat-tolerant transgenic orchardgrass plants.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.20 no.1
• /
• pp.7-12
• /
• 2000

This study was carried out to improve the ability of embryo formation and the efficiency of plant regeneration from suspension cultured cells of seed derived calli of orchardgrass (Dactylis glomerata L.). The frequency of formation of round cell and cell colonies was highest at 50 days after suspension culture in $N_6$ medium supplemented with $4\;g/{\ell}$ casein hydrolysate (CH), $20\;g/{\ell}$ sucrose and $30\;g/{\ell}$ sorbitol. The highest frequency of plant regeneration and somatic embryo formation was obtained from suspension cultured cells of 60 days. Addition of CH ($4\;g/{\ell}$) in suspension culture medium gave the highest frequency of embryo formation (39.6%) and plant regeneration (73.0%).

• Proceedings of the Korean Society of Grassland Science Conference
• /
• 2000.09a
• /
• pp.102.2-102
• /
• 2000

• Korean Journal of Plant Tissue Culture
• /
• v.28 no.2
• /
• pp.75-79
• /
• 2001

An experiment was carried out to introduce OsHSP17.9, a low molecular HSP gene isolated from rice plant to orchardgrass (Dactylis glomerata L.) using Agrobacterium. Mature seed-derived calli of orchardgrass were co-cultured with Agrobacterium tumefaciens EHA101 harboring the plasmid pIG-HSP17.9 for transformation. Calli selected by hygromycin were transferred to N$_{6}$ medium containing 1 mg/L NAA, 5 mg/L kinetin, 250 mg/L cefotaxime and 50 mg/L hygromycin and several hygromycin resistant plants were obtained. Stable incorporation of the introduced OsHSP17.9 to the genome of the hygromycin resistant plants was confirmed by PCR and Southern blot analysis. Transformation efficiency was variable between cultivars in which it was 16.5% in Potomac and 8.0% in Frontier. Constitutive expression of the transgene in the transformed orchardgrass tissues was identified by Northern blot analysis but transcript levels were different among individual plants.s.

• Proceedings of the Korean Society of Grassland Science Conference
• /
• 2001.06a
• /
• pp.83.2-84
• /
• 2001

• Journal of Plant Biotechnology
• /
• v.30 no.4
• /
• pp.341-346
• /
• 2003

In an effort to optimize tissue culture conditions for genetic transformation of orchardgrass (Dactylis glomerata L.), an efficient and high-frequency plant regeneration system from seed-derived calli was established. Embryogenic calli induced on MS medium containing 3mg/L 2,4-D and 0.1mg/L BA had significantly improved regeneration ability. Plant regeneration rate was 92％ when embryogenic calli were cultured on N6 medium supplemented with 1mg/L 2,4-D and 3mg/L BA. Among three kinds of medium, MS and N6 medium were optimal for embryogenic callus induction and plant regeneration, respectively. Ho difference in callus induction frequency was observed among four cultivars of orchardgrass, however, "Roughrider" cultivar showed higher regenerability with the frequency of 61％. Addition of maltose to the regeneration medium as a carbon source dramatically increased regeneration frequency up to 69％. A short tissue culture period and high-frequency regeneration system would be beneficial for molecular breeding of orchardgrass through genetic transformation.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.10 no.3
• /
• pp.141-146
• /
• 1990

Changes in dry matter yield, crude components, nutrients uptake($P_2O_5$, $K_2O$, CaO, MgO) and sulphur containing amino acid(cysteine, methionine) of orchardgrass(Dacty1is glomerata. L) and alfalfa(A4edicago sativa. L) by gypsum application(as sulphur source, 0, 2. 5, 10, 20kg SIlOa) were investigated to understand the effect of sulphur on herbage production in pasture, which was established in 1987 as means of hand broadcasting. The effect of gypsum on dry matter yield at different cutting times during growing seasons has not been found both in orchardgrass and in alfalfa, but in respect to annual total dry matter yield there were increment in herbage yield (P<0.05) of alfalfa at 5, 10, 20kg SIlOa in 1989 and the amounts of sulphur taken up in herbage slightly increased according to the rates of gypsum application. Maximum apparent recovery of sulphur was 7.55% at 2kg SIlOa in orchardgrass and was 17.8% at 5kg S/lOa in alfalfa. There were no any great differences in the content of crude components of both species and this trend was similar with the mineral contents of orchardgrass. But in alfalfa, the amounts of $K_2O$, CaO, and $P_2O_5$ taken up were increased by gypsum application and the increment in the amounts of minerals taken up in herbage at 20kg SIlOa were 14.9 of $K_2O$, 9.1 of CaO, and 2.5kgIlOa of $P_2O_5$ as compared to those of at untreated plot. Cysteine and methionine were not influenced by gypsum applicaton not only in orchardgrass but also cysteine in alfalfa, however, the content of methionine in alfalfa was slightly increased at 2, 5, lOkg SIlOa and at 20kg SIlOa was reverse.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.10 no.1
• /
• pp.1-9
• /
• 1990

Tillering of orchardgrass during establishment year was considered as an important factor for ground cover and forage yield. Field experiment was conducted to examine seasonal fluctuation of tiller number in three cultivars of orchardgrass; Potomac, Hall-mark, and Sumas, after transplanting with five planting density. Also, effects of plant density and nitrogen application in cultivar, Potomac were studied on tillering, forage yield and various growth characteristics in pot experiment Tillers per plant decreased with increased density and showed seasonal variations. It increased up to July, decreased during summer months, and increased again from October. Sumas had less tillers than other cultivars, especially at high density. Tillers per unit area kept high during spring. After then, there was stady decrease through summer and remained constant without affecting by planting density. Nitrogen application increased tillers per unit area in Potomac up to 20kg/10a, but decreased its tiller production at 40kg/lOa. Maximum number of tiller was obtained in the combinations of 20kg application of nitrogen with higher density than 324 plant per square meter. Forage yield increased as nitrogen application increased, while it did not respond to plant density. Forage yield had high linear correlation between LAI, leaf number with a peak at 2800 culrns. For high forage yielding, it was suggested to obtain optimum number of tillers per unit area in early season as possible, applying of adequate amount of nitrogen with relative high density depend on cultural condition.