• 제목/요약/키워드: DSS model

검색결과 149건 처리시간 0.031초

Diversity of Deep-sea Piezophiles and Their Molecular Adaptations to High-pressure Environment

  • Kato, Chiaki;Sato, Takako;Tamegai, Hideyuki;Nakasone, Kaoru
    • 한국미생물학회:학술대회논문집
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    • 한국미생물학회 2007년도 International Meeting of the Microbiological Society of Korea
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    • pp.80-82
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    • 2007
  • We have isolated numerous cold deep-sea adapted microorganisms (piezophilic, formerly referred to as "barophilic" bacteria) using deep-sea research submersibles. Many of the isolates are novel psychrophilic bacteria, and we have identified several new piezophilic species, i.e., Photobacterium profundum, Shewanella violacea, Moritella japonica, Moritella yayanosii, Psychromonas kaikoi, and Colwellia piezophila. These piezophiles are involving to five genera in gamma-Proteobacteria subgroup and produce significant amounts of unsaturated fatty acids in their cell membrane fractions to maintain the membrane fluidity in cold and high-pressure environments. Piezophilic microorganisms have been identified in many deep-sea bottoms of many of the world oceans. Therefore, these microbes are well distributed on our planet. One of the isolated deep-sea piezophiles, Shewanella violacea strain DSS12 is a psychrophilic, moderately piezophilic bacterium from a sediment sample collected at the Ryukyu Trench (depth: 5,110 m), which grows optimally at 30 MPa and $8^{\circ}C$ but also grows at atmospheric pressure (0.1 MPa) and $8^{\circ}C$. We have examined this strain to elucidate the molecular basis for gene regulation at different pressure conditions because this strain is useful as a model bacterium for comparing the various features of bacterial physiology under pressure conditions. In addition, we completed the sequencing of the entire genome of this piezophilic bacterium and we expect that many biotechnologically useful genes will be identified from the genome information.

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Homology Modeling and Molecular Docking Study of Translationally Controlled Tumor Protein and Artemisinin

  • Chae, Jin-Sun;Choi, In-Hee;Kim, Choon-Mi
    • Archives of Pharmacal Research
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    • 제29권1호
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    • pp.50-58
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    • 2006
  • Translationally controlled tumor protein (TCTP), also known as histamine releasing factor (HRF), is found abundantly in different eukaryotic cell types. The sequence homology of TCTP between different species is very high, belonging to the MSS4/DSS4 superfamily of proteins. TCTP is involved in both cell growth and human late allergy reaction, as well as having a calcium binding property; however, its primary biological functions remain to be clearly elucidated. In regard to many possible functions, the TCTP of Plasmodium falciparum (Pf) is known to bind with an antimalarial agent, artemisinin, which is activated by heme. It is assumed that the endoperoxide-bridge of artemisinin is opened up by heme to form a free radical, which then eventually alkylates, probably to the Cys14 of PfTCTP. Study of the docking of artemisinin with heme, and subsequently with PfTCTP, was carried out to verify the above hypothesis on the basis of structural interactions. The three dimensional (3D) structure of PfTCTP was built by homology modeling, using the NMR structure of the TCTP of Schizosaccharomyces pombe as a template. The quality of the model was examined based on its secondary structure and biological function, as well as with the use of structure evaluating programs. The interactions between artemisinin, heme and PfTCTP were then studied using the docking program, FlexiDock. The center of the peroxide bond of artemisinin and the Fe of heme were docked within a short distance of $2.6{\AA}$, implying the strong possibility of an interaction between the two molecules, as proposed. When the activated form of artemisinin was docked on the PfTCTP, the C4-radical of the drug faced towards the sulfur of Cys14 within a distance of $2.48{\AA}$, again suggesting the possibility of alkylation having occurred. These results confirm the proposed mechanism of the antimalarial effect of artemisinin, which will provide a reliable method for establishing the mechanism of its biological activity using a molecular modeling study.

Changes in Physiological Activity of Gardenia Fructus by Roasting Treatment

  • Park, Ji Sun;Choi, Ha Kyoung;Kang, Jeong Eun;Shin, Yong Wook;Lee, In Ah
    • 인간식물환경학회지
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    • 제23권4호
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    • pp.455-464
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    • 2020
  • Background and objective: This study was conducted to examine changes in the composition and physiological activity of Gardenia Fructus after being roasted. Methods: The antioxidant, anti-inflammatory and antibacterial activity of Gardenia Fructus was evaluated using the Gardenia Fructus (GF) and roasted Gardenia Fructus (RGF) ethanol extracts, and their components were analyzed through HPLC. Results: As a result, it was confirmed that the content of gardenoside and geniposide decreased and the content of genipin increased when GF was roasted. The total content of polyphenols was 54.5 ± 2.18 mg gallic acid equivalents (GAE) per gram of the GF extract and 69.6 ± 0.36 mg GAE per gram of the RGF extract. As a result of evaluating 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, both the GF and RGF extracts showed the similar activity to ascorbic acid at the concentrations of 1 mg/mL or higher. In RAW 264.7 macrophages stimulated by lipopolysaccharides (LPS), the RGF extract showed a higher effect of reducing NO production, and significantly reduced the expression of an inflammatory cytokine, IL-6. As a result of evaluating the antimicrobial activity, the RGF extract showed higher antimicrobial activity against Escherichia coli and Bacillus subtilis. In the dextran sulfate sodium salt (DSS) induced inflammatory bowel disease mouse model, the RGF extract reduced the weight of the spleen, and both the GF and RGF extracts reduced the number of bacteria in the colon. Conclusion: Therefore, it has been confirmed through this study that roasting at a high temperature changes the main components of the GF extract and increases its biological activity. The RGF extract is expected to be used as a natural material with antioxidant, anti-inflammatory and antibacterial effects.

염증성 장질환 모델 및 크론병 환자에서의 점막상피 HuR 단백질의 변화 분석 (Tissue Distribution of HuR Protein in Crohn's Disease and IBD Experimental Model)

  • 최혜진;박재홍;박지연;김주일;박성환;오창규;도기헌;송보경;이승준;문유석
    • 생명과학회지
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    • 제24권12호
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    • pp.1339-1344
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    • 2014
  • 염증성 장질환은 점막의 만성적 궤양과 염증을 동반하는 면역질환으로 알려져 있으며, 특히 TNF${\alpha}$와 같은 염증성 사이토카인은 주요한 생물학적 치료의 표적으로 이용되고 있다. 염증성 사이토카인의 유전자발현에서 전사물의 안정화는 매우 중요한 조절과정이며, 특히 본 연구에서는 이 안정화에 핵심적인 단백질인 HuR의 발현과 조직 분포에 대하여 동물모델과 환자의 조직에서 분석하였다. DSS를 처리함으로 유도되는 장염증 동물 모델에서 HuR 단백질의 발현량이 높았음을 확인했고, 점막의 상피조직 및 선조직 상피세포에서 상대적인 발현이 증대되었다. 또한 단백질의 활성측면에서 세포질로 이동된 HuR 단백질의 양도 상대적으로 증가하였다. 공간분포적으로 보면 DSS에 의한 화학적 점막자극에 의하여 초기에는 villi 하부에서의 발현정도가 상대적으로 villus 말단에 비하여 높게 유지되었다. 크론병 환자의 생검을 통하여 정상부위와 병변부위에서 HuR 단백질을 비교분석 하였다. 크론병 환자들의 병변에서는 지속적으로 HuR의 발현이 증대되어 있음을 확인했으며, 동물조직과 유사하게 병변부위의 장관상피세포 및 선 상피에서 주로 발현양이 높았다. 이러한 결과는 염증성 장질환에서의 HuR 단백질이 초기 염증성 인자의 발현에 중요한 역할이 예상되며, 구체적인 분자기전의 규명도 향후 기대된다. 이를 근간으로 하여 염증성 장질환의 진단과 치료의 표적개발에서 유용하게 응용하고자 한다.

Effect of Dietary Selenium on the Colon Carcinogenesis in Male ICR Mice

  • Cho, Min-Haeng;Kim, Jun-Hyeong;Hue, Jin-Joo;Kang, Bong-Su;Park, Hyun-Ji;Nam, Sang-Yoon;Yun, Young-Won;Kim, Jong-Soo;Jeong, Jae-Hwang;Lee, Beom-Jun
    • 한국식품위생안전성학회지
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    • 제25권3호
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    • pp.269-277
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    • 2010
  • 본 연구에서는 azoxymethane (AOM)과 dextran sodium sulfate (DSS)로 유도된 대장 발암과정에 대한 셀레늄의 방어 효과를 조사하였다. 셀레늄 결핍(0.02 ppm Se), 정상(0.1 ppm Se), 과다(0.5 ppm Se)사료를 12주간 식이로 급여하여 혈액검사와 대장암 발생의 초기단계인 aberrant crypt foci (ACF)수를 측정했으며, 암 발생율을 조사하였다. ICP-AES를 사용하여 간의 셀레늄 농도를 측정하였으며, 또한 셀레늄포함 항산화효소인 glutathione peroxidase (GPx) 활성을 알아보았다. 또한 TUNEL assay와 PCNA, $\beta$-catenin에 대한 면역조직 염색을 수행하였다. ACF 수 및 종양 발생률에 있어서, 셀레늄과다사료를 급여한 군이 정상셀레늄사료를 급여한 군보다 낮았으며, 셀레늄결핍사료를 급여한 군은 오히려 ACF 수 및 종양 발생률이 높았다. GPx 활성은 셀레늄의 섭취가 과다한 군에서 높게 나타났으며, 이 때, TUNEL에서 apoptotic positive cell이 증가하는 것을 확인했다. 또한 셀레늄의 섭취가 과다한 군에서 PCNA와 $\beta$-catenin의 발현이 감소됨을 볼 수 있었다. 본 마우스 모델실험에서 셀레늄은 여러 기전에 의해 대장암 발생을 억제할 수 있을 것으로 사료된다.

RAW 264.7 대식세포와 궤양성 대장염 마우스 모델에서의 들깨의 항염증 효과 (Anti-inflammatory Activity of Perilla frutescens Britton Seed in RAW 264.7 Macrophages and an Ulcerative Colitis Mouse Model)

  • 이유나;송보람;주지형
    • 한국식품과학회지
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    • 제46권1호
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    • pp.61-67
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    • 2014
  • 본 연구에서는 생 들깨와 볶은 들깨의 염증매개물질 감소 효과를 RAW 264.7 대식 세포와 궤양성 대장염이 유도된 마우스를 이용하여 비교 분석하고자 하였다. LPS 처리에 의해 활성화된 RAW 264.7 대식세포에서 들깨의 에탄올 추출물은 볶음 여부와는 관계없이 NO, IL-6, TNF-${\alpha}$와 같은 염증매개물질 수준을 유의적으로 감소시키는 효과(대조군 대비 45-85% 수준)가 있었다. 반면 DSS 처리에 의해 궤양성 대장염이 유도된 마우스 모델에서는, 볶은 들깨 식이(1%)만이 대장의 $PGE_2$, $LTB_4$와 같은 염증매개물질 수준을 유의적으로 감소시키는 효과(각각 대조군 대비 34%, 58% 수준)가 있었다. 이와 같은 연구 결과를 종합하여 보면, 볶은 들깨는 in vitro 항염성 뿐 아니라 in vivo 항염성을 가지는 것으로 판단된다. 앞으로 들깨의 볶음 과정에서 생성된 항염 기능 성분들을 분리 동정하는 연구와 볶은 들깨의 항염성과 관련된 기전에 관한 후속 연구가 지속적으로 이루어진다면 볶은 들깨가 대장염을 포함한 여러 염증관련 질병 예방에 유용한 소재로 이용될 수 있을 것으로 기대된다.

참여자관점에서 공급사슬관리 시스템의 성공에 영향을 미치는 요인에 관한 실증연구 (An Empirical Study on the Determinants of Supply Chain Management Systems Success from Vendor's Perspective)

  • 강성배;문태수;정윤
    • Asia pacific journal of information systems
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    • 제20권3호
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    • pp.139-166
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    • 2010
  • The supply chain management (SCM) systems have emerged as strong managerial tools for manufacturing firms in enhancing competitive strength. Despite of large investments in the SCM systems, many companies are not fully realizing the promised benefits from the systems. A review of literature on adoption, implementation and success factor of IOS (inter-organization systems), EDI (electronic data interchange) systems, shows that this issue has been examined from multiple theoretic perspectives. And many researchers have attempted to identify the factors which influence the success of system implementation. However, the existing studies have two drawbacks in revealing the determinants of systems implementation success. First, previous researches raise questions as to the appropriateness of research subjects selected. Most SCM systems are operating in the form of private industrial networks, where the participants of the systems consist of two distinct groups: focus companies and vendors. The focus companies are the primary actors in developing and operating the systems, while vendors are passive participants which are connected to the system in order to supply raw materials and parts to the focus companies. Under the circumstance, there are three ways in selecting the research subjects; focus companies only, vendors only, or two parties grouped together. It is hard to find researches that use the focus companies exclusively as the subjects probably due to the insufficient sample size for statistic analysis. Most researches have been conducted using the data collected from both groups. We argue that the SCM success factors cannot be correctly indentified in this case. The focus companies and the vendors are in different positions in many areas regarding the system implementation: firm size, managerial resources, bargaining power, organizational maturity, and etc. There are no obvious reasons to believe that the success factors of the two groups are identical. Grouping the two groups also raises questions on measuring the system success. The benefits from utilizing the systems may not be commonly distributed to the two groups. One group's benefits might be realized at the expenses of the other group considering the situation where vendors participating in SCM systems are under continuous pressures from the focus companies with respect to prices, quality, and delivery time. Therefore, by combining the system outcomes of both groups we cannot measure the system benefits obtained by each group correctly. Second, the measures of system success adopted in the previous researches have shortcoming in measuring the SCM success. User satisfaction, system utilization, and user attitudes toward the systems are most commonly used success measures in the existing studies. These measures have been developed as proxy variables in the studies of decision support systems (DSS) where the contribution of the systems to the organization performance is very difficult to measure. Unlike the DSS, the SCM systems have more specific goals, such as cost saving, inventory reduction, quality improvement, rapid time, and higher customer service. We maintain that more specific measures can be developed instead of proxy variables in order to measure the system benefits correctly. The purpose of this study is to find the determinants of SCM systems success in the perspective of vendor companies. In developing the research model, we have focused on selecting the success factors appropriate for the vendors through reviewing past researches and on developing more accurate success measures. The variables can be classified into following: technological, organizational, and environmental factors on the basis of TOE (Technology-Organization-Environment) framework. The model consists of three independent variables (competition intensity, top management support, and information system maturity), one mediating variable (collaboration), one moderating variable (government support), and a dependent variable (system success). The systems success measures have been developed to reflect the operational benefits of the SCM systems; improvement in planning and analysis capabilities, faster throughput, cost reduction, task integration, and improved product and customer service. The model has been validated using the survey data collected from 122 vendors participating in the SCM systems in Korea. To test for mediation, one should estimate the hierarchical regression analysis on the collaboration. And moderating effect analysis should estimate the moderated multiple regression, examines the effect of the government support. The result shows that information system maturity and top management support are the most important determinants of SCM system success. Supply chain technologies that standardize data formats and enhance information sharing may be adopted by supply chain leader organization because of the influence of focal company in the private industrial networks in order to streamline transactions and improve inter-organization communication. Specially, the need to develop and sustain an information system maturity will provide the focus and purpose to successfully overcome information system obstacles and resistance to innovation diffusion within the supply chain network organization. The support of top management will help focus efforts toward the realization of inter-organizational benefits and lend credibility to functional managers responsible for its implementation. The active involvement, vision, and direction of high level executives provide the impetus needed to sustain the implementation of SCM. The quality of collaboration relationships also is positively related to outcome variable. Collaboration variable is found to have a mediation effect between on influencing factors and implementation success. Higher levels of inter-organizational collaboration behaviors such as shared planning and flexibility in coordinating activities were found to be strongly linked to the vendors trust in the supply chain network. Government support moderates the effect of the IS maturity, competitive intensity, top management support on collaboration and implementation success of SCM. In general, the vendor companies face substantially greater risks in SCM implementation than the larger companies do because of severe constraints on financial and human resources and limited education on SCM systems. Besides resources, Vendors generally lack computer experience and do not have sufficient internal SCM expertise. For these reasons, government supports may establish requirements for firms doing business with the government or provide incentives to adopt, implementation SCM or practices. Government support provides significant improvements in implementation success of SCM when IS maturity, competitive intensity, top management support and collaboration are low. The environmental characteristic of competition intensity has no direct effect on vendor perspective of SCM system success. But, vendors facing above average competition intensity will have a greater need for changing technology. This suggests that companies trying to implement SCM systems should set up compatible supply chain networks and a high-quality collaboration relationship for implementation and performance.

NQO1 (NAD(P)H:quinone oxidoreductase 1)에 의한 대식세포 활성화 억제 (Inhibitory Effect of NAD(P)H:Quinone Oxidoreductase 1 on the Activation of Macrophages)

  • 훙지;장펑;윤이나;김호
    • 생명과학회지
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    • 제27권8호
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    • pp.873-878
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    • 2017
  • 본 연구는 대식세포 활성화 과정에서 NQO1의 역할을 확인하는 것이다. 대식세포의 활성화 정도는 배양액으로 분비하는 IL-6와 $TNF-{\alpha}$ 양을 측정하여 평가하였다. 먼저 NQO1 WT 생쥐와 NQO1 KO 생쥐에서 각각 분리한 복강대식세포의 활성화 정도를 비교해 보았다. 특이하게도 NQO1 KO 복강대식세포가 NQO1 WT에 비해서 더 높게 활성화되어 있었다. 또한 일반 생쥐의 복강대식세포에 NQO1 억제제(dicumarol)을 처치한 경우에도 강한 활성이 유도됨을 확인하였다. Dicumarol을 처치한 RAW264.7 (대식세포주)에 서도 강한 활성화가 관찰되었다. 이는 NQO1이 대식세포의 활성화 과정을 억제하는 경로와 연관되어 있음을 보여준다. 더욱이 dicumarol을 처치하여 NQO1의 기능을 억제시킨 다양한 대식세포에서 $I{\kappa}B$ 단백질이 유의하게 감소한다는 사실을 확인하였다. 대식세포 활성화 과정을 매개하는 주요 신호분자가 $NF{\kappa}B$이며 이 분자에 대한 억제자가 $I{\kappa}B$라는 사실들을 감안할 때, NQO1의 기능이 $I{\kappa}B$ 단백질변성 억제와 연관되어 있으며 이를 통해 대식세포의 활성화를 차단했을 가능성이 있다. 본 연구는 향 후 대식세포 활성화 과정을 조절하는 NQO1의 역할을 규명하는데 있어서 중요한 기초 결과가 될 것이다.

홍수조절지 건설 후 사격장 주변지역의 위해성예측 사례연구 (Prediction of Potential Risk Posed by a Military Gunnery Range after Flood Control Reservoir Construction)

  • 류혜림;한준경;남경필;배범한
    • 한국지하수토양환경학회지:지하수토양환경
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    • 제12권1호
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    • pp.87-96
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    • 2007
  • 본 연구는 경기도 소재 모 사격장의 주요 오염물질 여섯 가지에 대하여 홍수조절지가 건설된 이후 사격장 주변지역의 위해성을 예측하기 위하여 수행되었다. 해당 지역의 주요 오염물질 중 인체에 독성이 있는 화약물질 3종과 중금속 3종을 대상물질로 선정하였으며, 오염의 정도와 토지의 이용용도에 따라 네 지역으로 나누어 평가를 실시하였다. 위해성이 과대평가되는 것을 피하기 위하여 대상지역의 인문사회학적 특성 및 지반환경공학적 특성을 기반으로 노출경로모델(Conceptual Site Model)을 작성하였으며 각 노출경로에 따른 오염물질 이동모델 및 위해성 평가는 API's DSS(American Petroleum Institute's Decision Support System)를 이용하였다. 수용체나 지역의 특성을 하나의 값으로 대표할 수 없는 경우 위해성이 과소평가되는 것을 방지하기 위하여 가장 안전한 값을 사용하였다. 위해성 예측결과, 피탄지인 Ac 지역에서 TNT(Tri-Nitro-Toluene)와 카드뮴의 비발암위해도가 1을 조금 넘고, RDX(Royal Demolition Explosives)의 경우 50이 넘어, 대상지역 전체에 대한 총 비발암위해도는 62.828라는 매우 큰 값을 나타내었다. 한편, 발암위해도는 납이 약 $5\;{\times}\;10^{-4}$, 카드뮴이 약 $1\;{\times}\;10^{-3}$으로, 일반적으로 받아들여지는 발암위해도의 적정수준인 $10^{-4}{\sim}10^{-6}$에 비하여 $5{\sim}10$ 배 정도 크게 평가되었다. 이러한 위해성평가 결과를 통하여, 해당지역에 홍수조절지를 건설하기 전에 비발암물질과 발암물질 모두에 대한 즉각적인 복원사업이 진행되어야 하며, 홍수조절지 건설 후에도 사격장이 계속 운영될 경우 적절한 오염물질의 관리정책이 필요함을 알 수 있었다.