• Journal of Microbiology and Biotechnology
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• v.20 no.12
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• pp.1637-1646
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• 2010

The bacterium Deinococcus radiodurans is one of the most resistant organisms to ionizing radiation and other DNA-damaging agents. Although, at present, 30 Deinococcus species have been identified, the whole-genome sequences of most species remain unknown, with the exception of D. radiodurans (DRD), D. geothermalis, and D. deserti. In this study, comparative genomic hybridization (CGH) microarray analysis of three Deinococcus species, D. radiopugnans (DRP), D. proteolyticus (DPL), and D. radiophilus (DRPH), was performed using oligonucleotide arrays based on DRD. Approximately 28%, 14%, and 15% of 3,128 open reading frames (ORFs) of DRD were absent in the genomes of DRP, DPL, and DRPH, respectively. In addition, 162 DRD ORFs were absent in all three species. The absence of 17 randomly selected ORFs was confirmed by a Southern blot. Functional classification showed that the absent genes spanned a variety of functional categories: some genes involved in amino acid biosynthesis, cell envelope, cellular processes, central intermediary metabolism, and DNA metabolism were not present in any of the three deinococcal species tested. Finally, comparative genomic data showed that 120 genes were Deinococcus-specific, not the 230 reported previously. Specifically, ddrD, ddrO, and ddrH genes, previously identified as Deinococcus-specific, were not present in DRP, DPL, or DRPH, suggesting that only a portion of ddr genes are shared by all members of the genus Deinococcus.

• Earthquakes and Structures
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• v.23 no.3
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• pp.259-269
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• 2022

In this study, we introduce a canonical correlation analysis method to accurately assess the tunnel damage potential of ground motion. The proposed method can retain information relating to the initial variables. A total of 100 ground motion records are used as seismic inputs to analyze the dynamic response of three different profiles of tunnels under deep and shallow burial conditions. Nine commonly used ground motion parameters were selected to form the canonical variables of ground motion parameters (GMP_CCA). Five structural dynamic response parameters were selected to form canonical variables of structural dynamic response parameters (DRP_CCA). Canonical correlation analysis is used to maximize the correlation coefficients between GMP_CCA and DRP_CCA to obtain multivariate ground motion parameters that can be used to comprehensively assess the tunnel damage potential. The results indicate that the multivariate ground motion parameters used in this study exhibit good stability, making them suitable for evaluating the tunnel damage potential induced by ground motion. Among the nine selected ground motion parameters, peck ground acceleration (PGA), peck ground velocity (PGV), root-mean-square acceleration (RMSA), and spectral acceleration (Sa) have the highest contribution rates to GMP_CCA and DRP_CCA and the highest importance in assessing the tunnel damage potential. In contrast to univariate ground motion parameters, multivariate ground motion parameters exhibit a higher correlation with tunnel dynamic response parameters and enable accurate assessment of tunnel damage potential.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.21 no.5
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• pp.59-69
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• 2011

Since power analysis attack which uses a characteristic that power consumed by crypto device depends on processed data has been proposed, many logics that can block these correlation originally have been developed. DRP logic has been adopted by most of logics maintains power consumption balanced and reduces correlation between processed data and power consumption. However, semi-custom design is necessary because recently design circuits become more complex than before. This design method causes unbalanced design pattern that makes DRP logic consumes unbalanced power consumption which is vulnerable to power analysis attack. In this paper, we have developed new logic style which adds another discharge phase to discharge two output nodes at the same time based on DyCML to remove this unbalanced power consumption. Also, we simulated 1bit fulladder to compare proposed logic with other logics to prove improved performance. As a result, proposed logic is improved NED and NSD to 60% and power consumption reduces about 55% than any other logics.

• Journal of Life Science
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• v.31 no.5
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• pp.464-474
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• 2021

Inflammation induced by metabolic syndromes, cancers, injuries, and sepsis can alter cellular metabolism by reducing mitochondrial function via oxidative stress, thereby resulting in neuropathy and muscle atrophy. In this study, we investigated whether butyrate, a short chain fatty acid produced by gut microbiota, could prevent mitochondrial dysfunction and muscle atrophy induced by lipopolysaccharide (LPS) in the C2C12 cell line. LPS-activated MAPK signaling pathways increased the levels of the mitochondrial fission signal, p-DRP1 (Ser616), and the muscle atrophy marker, atrogin 1. Interestingly, butyrate significantly inhibited the phosphorylation of JNK and p38 and reduced the atrogin 1 level in LPS-treated C2C12 cells while increasing the phosphorylation of DRP1 (Ser637) and levels of mitofusin2, which are both mitochondrial fusion markers. Next, we investigated the effect of MAPK inhibitors, finding that butyrate had the same effect as JNK inhibition in C2C12 cells. Also, butyrate inhibited the LPS-induced expression of pyruvate dehydrogenase kinase 4 (PDK4), resulting in decreased PDHE1α phosphorylation and lactate production, suggesting that butyrate shifted glucose metabolism from aerobic glycolysis to oxidative phosphorylation. Finally, we found that these effects of butyrate on LPS-induced mitochondrial dysfunction were caused by its antioxidant effects. Thus, our findings demonstrate that butyrate prevents LPS-induced muscle atrophy by improving mitochondrial dynamics and metabolic stress via the inhibition of JNK phosphorylation. Consequently, butyrate could be used to improve LPS-induced mitochondrial dysfunction and myopathy in sepsis.

• International Journal of Stem Cells
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• v.16 no.2
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• pp.123-134
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• 2023

Objective: The heart contains a pool of c-kit⁺ progenitor cells which is believed to be able to regenerate. The differentiation of these progenitor cells is reliant on different physiological cues. Unraveling the underlying signals to direct differentiation of progenitor cells will be beneficial in controlling progenitor cell fate. In this regard, the role of the mitochondria in mediating cardiac progenitor cell fate remains unclear. Specifically, the association between changes in mitochondrial morphology with the differentiation status of c-kit⁺ CPCs remains elusive. In this study, we investigated the relationship between mitochondrial morphology and the differentiation status of c-kit⁺ progenitor cells. Methods and Results: c-kit⁺ CPCs were isolated from 2-month-old male wild-type FVB mice. To activate differentiation, CPCs were incubated in α-minimal essential medium containing 10 nM dexamethasone for up to 7 days. To inhibit Drp1-mediated mitochondrial fragmentation, either 10 μM or 50 μM mdivi-1 was administered once at Day 0 and again at Day 2 of differentiation. To inhibit calcineurin, either 1 μM or 5 μM ciclosporin-A (CsA) was administered once at Day 0 and again at Day 2 of differentiation. Dexamethasone-induced differentiation of c-kit⁺ progenitor cells is aligned with fragmentation of the mitochondria via a calcineurin-Drp1 pathway. Pharmacologically inhibiting mitochondrial fragmentation retains the undifferentiated state of the c-kit⁺ progenitor cells. Conclusions: The findings from this study provide an alternative view of the role of mitochondrial fusion-fission in the differentiation of cardiac progenitor cells and the potential of pharmacologically manipulating the mitochondria to direct progenitor cell fate.

• Biomedical Science Letters
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• v.18 no.2
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• pp.112-122
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• 2012

A gradual change of molecules that are related in fission and fusion is occurred during aging process. Although aging effects on mitochondrial fusion and fission are investigated, it is still unclear that the extent of the change in mitochondria fusion and fission periodically. In this study we investigated the changes of mitochondrial proteins involved in fusion (Mfn2, Opa1) and fission (Drp1, Fis1) in the human gracilis muscle ranging from 10 to 50 years of age (n=40). The gracilis muscle showed a significant increase in muscle apoptotic changes in the age of 50s compared with 10s by using in situ terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL). The expression levels of Drp1 and Fis1 (P<0.01, P<0.05) mRNA were significantly elevated and the Mfn2 and Opa1 (P<0.01, P<0.05) levels were decreased from older individuals. The ratio of fission and fusion was altered and the level of increment of fission gene was greater than fusion gene decrement in the age of 50s. These findings suggest that changes of mitochondrial fusion and fission proteins related with aging might contribute to aged muscle apoptosis.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 2004.05a
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• pp.454-457
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• 2004

The high degree of uncertainty in customer demands forces companies of today to equip with a flexible and adaptable structural organization. It also has an influence on the configuration of the company's supply chains, since the members in supply chains are dependent to the goods customers need. If a company can not come up with the customers' requirements and their level of satisfaction, the competitiveness of the company will be weaken in the market places. To meet such conditions, therefore, reconfiguration of supply chains should be considered as an important factor in a complex, dynamic, and fluctuating market environment. In this paper, therefore, we have adapted a fractal concept to SCM to facilitate reconfiguration of supply chains. To do so, we have first introduce fractal-based SCM, referred to as fSCM. Then, we have described definitions of the reconfigurability in supply chains and the methodology of Dynamic Restructuring Process (DRP) in supply chains including specific strategies and constraints. An exemplary model will also be briefly illustrated to demonstrate how supply chains conduct DRP autonomously.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.19 no.5
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• pp.1091-1096
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• 2015

In this paper, WiMedia Distributed-MAC protocol is adopted for development of multi-hop N-screen wireless services. Furthermore, to provide the multi-hop OSMU (One Source Multi Use) N-screen service through P2P streaming in the D-MAC protocol, a new multi-hop resource reservation scheme is proposed and analyzed to reduce end to end delay. For this Multi-hop resource reservation scheme, a new MSCDRP (Multi-Stage Cooperative DRP) IE is proposed. Simulation results show that our multi-hop MSCDRP scheme reduces the end to end delay and increases throughput at various multi-hop N-screen network environment.

• Journal of The Korean Society of Agricultural Engineers
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• v.55 no.3
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• pp.13-24
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• 2013

Agriculture is affected directly by climate conditions and changes, and it is necessary to understand the impact of climate change on agricultural reservoirs which are the main water resources for paddy fields in Korea. This study aimed to evaluate the impact of climate change on the anti-drought capacity including water supply capability (WSC) and drought response ability (DRA) of agricultural reservoirs based on RCP (Representative Concentration Pathway) 4.5 and 8.5 scenarios of CanESM2 (The Second Generation Earth System Model) provided by CCCma (Canadian Center for Climate Modeling and Analysis). The WSC and DRA were estimated using frequency analysis and runs theory. The six reservoirs (Yooshin, Nogok, Kumsung, Songgok, Gapyung, Seoma) were selected considering geographical characteristics and design criteria of reservoir capacity. In case of Seoma reservoir, more than 10 year drought return period (DRP), the variation of the WSC was estimated larger than the others. In case of Yooshin reservior (2~5 DRP) DRC was decreased in 2025s under RCP8.5. These results could be utilized for agricultural reservoirs management and future design criteria considering climate change impacts on paddy irrigation.

• Korean Chemical Engineering Research
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• v.50 no.2
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• pp.304-309
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• 2012

Size verification of small and large bubbles in a bubble column was investigated by employing the dynamic gas disengagement (DGD) method and dual electrical resistivity probe (DRP) method, simultancously. The holdups of large and small bubbles in the bubble column in a given operating condition were obtained by means of the DGD method by measuring the pressure drop variation in the column with a variation of time after stopping the gas input into the column. The size and frequency of bubbles were measured by the DRP method in the same operating condition, from which the bubble holdup of each range of size was obtained. The verification of size in determining the large or small bubbles was decided by comparing the holdups of large or small bubbles measured by the DGD method with that measured by the DRP method. Filtered compressed air and tap water were used as a gas and a continuous liquid medium. The diameter and height of the bubble column were 0.102 m and 1.5 m, respectively. The demarcation size between the large and the small bubbles in the bubble column was 4.0~5.0 mm; the demarcation size was about 5.0 mm when the gas velocity was in the relatively low range, but about 4.0 mm when the gas velocity was in the relatively high range, within this experimental conditions.