• Bulletin of the Korean Chemical Society
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• 제31권12호
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• pp.3567-3572
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• 2010

Black pine barks from the southern region of Korea were extracted using pressurized hot water and the water soluble extracts were then separated in a stepwise fashion using a variety of solvents, column chromatography (CC), thin layer chromatography (TLC), and high pressure liquid chromatography (HPLC). The antioxidant activities of each fraction and the active compounds were determined based on the radical scavenging activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH), reductive potential of ferric ion, and total phenol contents. A DPPH test showed that the half maximal effective concentration ($EC_{50}$ value : $6.59{\pm}0.31\;{\mu}g/mL$) of the ethyl acetate fraction (ca. 0.67%) was almost the same as that of the control compounds and inversely proportional to the value of the total phenol contents. The cell viability of the water extracts was confirmed by methyl thiazol-2-yl-2,5-diphenyl tetrazolium bromide (MTT) with enzyme linked immune sorbent assay (ELISA). Catechin, epicatechin, quercetin and ferulic acid were isolated from the ethyl acetate fraction as active compounds and identified by nuclear magnetic resonance. The antioxidant activity as value of DPPH of each of the separated compounds was lower than the ethyl acetate fraction, and ferulic acid was the lowest among these compounds.

• 한국응용과학기술학회지
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• 제39권1호
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• pp.10-17
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• 2022

본 연구에서는 비건 인증(Vegan) 화장품 원료 7종 혼합추출물의 항산화능 및 미백효과를 확인하였다. 라디컬 소거능으로서 DPPH, ABTS, FRAP 실험, 항산화 물질 측정으로서 폴리페놀과 플라보노이드 함량 측정을 실시하였다. DPPH 실험에서는 105.74 mg ascorbic acid / g의 항산화능을 나타내었으며, ABTS 실험에서는 85.31 mg ascorbic acid / g의 항산화능을 나타내었다. FRAP에서는 7종 혼합추출물의 1 mg이 ascorbic acid 198.01±5.50 ㎍의 환원력을 보였다. 폴리페놀 함량은 30.19±0.75 mg/g으로 나타났으며, 플라보노이드 함량은 9.12±0.36 mg/g으로 나타났다. 한편 세포실험에서는 세포 독성과 미백활성을 알아보았다. 세포독성의 경우 20% 이하의 세포 독성을 보였으며, 100 ㎍/mL 농도에서 34.70±2.97%의 멜라닌 생성 억제능을 보여 7종 혼합추출물의 기능성 화장품 원료로서의 가능성을 확인하였다.

• 한국응용과학기술학회지
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• 제15권3호
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• pp.27-31
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• 1998

Antioxodative substances in Mulberry leaves were examined. Antioxidative substances in Mulberry leaves were extracted by 80% methanol agueous solution. Antioxidative activity of extract was determined by examining hydrogen donating ability on 1,1-diphenyl-2-picrylhydrazyl (DPPH) and the inhibitory effect on the formation of the peroxide from Linoleic acid in the test tube at $50^{\circ}C$. Antioxidative substance were, then, separated and indentified by thin layer chromatography(TLC), UV-Vis spectrum and High performance liquid chromatography(HPLC) methods. Hydrogen donating ability on DPPH and antioxidative ability on linoleic acid of the extracted antioxidative substance were higher than those of 100ppm butylated hydroxy toluene(BHT). The extracted antioxidative substances were separated by TLC using ethylacetate : chloroform : formic acid : water(8 : 1 : 1 : 1 v/v) as a solvent, and a spot at Rf=0.35 was detected. The spot was scraped from the plate, and extrated by methanol. The extract was analyzed by UV-Vis spetra and HPLC, and chlorogenic acid was identified as a antioxidative substance.

• Journal of Forest and Environmental Science
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• 제23권1호
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• pp.1-4
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• 2007

The bark of Juglans mandshurica Maxim. was collected, extracted with acetone-$H_2O$ (7:3, v/v), fractioned with n-hexane, $CH_2Cl_2$ and EtOAc, then each fraction was freeze-dried to give some powders. A portion of the EtOAc (28.4 g) fraction was chromatographed on a Sephadex LH-20 column eluting with a series of MeOH-$H_2O$ and EtOH-hexane mixture. Four gallotannins, gallic acid (1), ellagic acid (2), 1,2,4,6-tetra-O-galloyl-${\beta}$-D-glucose (3) and 1,2,3,4,6-penta-O-galloyl-${\beta}$-D-glucose (4), have been isolated from the EtOAc fraction Their structures were elucidated on the basis of chemical evidence and spectrometric analysis such as NMR and MS. The antioxidant activities on each fraction and the isolated gallotannins were evaluated by DPPH radical scavenging test.

• KSBB Journal
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• 제27권2호
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• pp.131-135
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• 2012

To develop potential cosmetic ingredients with antimicrobial and antioxidant activities of 4 Korean bamboo species (P. bambusoides, P. nigra var. henonis, P. pubescens and Sasa coreana) using three different extraction methods-water, ethanol and supercritical fluid extraction. Antimicrobial activities and DPPH assay have been examined. Among the antimicrobial activities against two test strains, Escherichia coli and Staphylococcus aureus, ethanol extracts of 3 bamboo trees, P. bambusoides, P. nigra var. henonis, and P. pubescens, showed stronger than those of supercritical extracts. However, 4 bamboo supercritical extracts showed dose-dependent increase in antioxidant activity by DPPH assay. These results suggest that water fraction of bamboo extracts may be useful for the cosmetic ingredient with low cytotoxicity.

• Archives of Pharmacal Research
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• 제29권3호
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• pp.199-202
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• 2006

From the leaves of Goniothalamus tenuifolius, a new natural product namely 3'-hydroxy-3,5,7,4'-tetramethoxyflavone (1) was isolated, along with seven other known compounds (2-8). Each of these isolates was evaluated for free radical scavenging activity on the DPPH decoloration test. The data obtained in this study suggested that the ortho 3',4'-diphenolic structure was essential for the activity of these flavonol derivatives.

• 한국식생활문화학회지
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• 제33권2호
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• pp.169-175
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• 2018

This study was conducted to investigate the quality characteristics of Yanggaeng prepared with different concentrations of mealworm powder: 0, 1, 2, and 3%. Regarding the proximate composition, moisture, crude lipid and ash contents showed no significant differences among groups, while crude protein contents increased as the mealworm powder increased. The value of lightness (L) and yellowness (b) were higher in 3% added groups than those of control groups. Consumer acceptance test showed no significant differences between the controls and added groups were observed in color, smell, taste and overall acceptability. The total polyphenol content ranged from 19.87 to 51.37 mg GAE/100 g, exhibiting the increase with increasing mealworm powder level. DPPH radical scavenging activity and reducing power increased as the amount of mealworm powder increased. In conclusion, it is suggested that mealworm powder can be incorporated into Yanggaeng up to 3% without affecting the sensory quality of Yanggaeng.

• Archives of Pharmacal Research
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• 제26권10호
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• pp.821-825
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• 2003

The effects of purple sweet potato anthocyanin (SPA) and Cordyceps mushroom extract (CME) on lipid peroxidation, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and cognitive deficits were examined. Both SPA and CME exhibited DPPH radical scavenging activities with similar potency. In contrast, only SPA was shown to effectively inhibit lipid peroxidation initiated by $Fe^{2+}$ and ascorbic acid in rat brain homogenates. Furthermore, SPA markedly enhanced cognitive performance, assessed by passive avoidance test in ethanol-treated mice. Combined treatments with SPA and CME did not significantly influence the effects of SPA alone. These results demonstrate that anthocyanin prepared from purple sweet potato exhibits memory enhancing effects, which may be associated with its antioxidant properties.

• 한국식품영양학회지
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• 제34권4호
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• pp.415-422
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• 2021

This study assessed the preparation and quality characteristics of cookies prepared from Taraxacum coreamm powder (TCP) (0, 2, 4, 6, 8%) substituting wheat flour. The pH of the cookie dough significantly decreased with increasing TCP. However the density of the cookie dough was not significantly different among the test groups. The baking loss rate of the groups with TCP was slightly lower, whereas the moisture content was higher than the control group. The spread factor of the cookies decreased significantly with increasing TCP. The hardness of the cookies increased as TCP increased. In Hunter's color value, the L and a value of the cookies decreased with increasing TCP, while the b value increased. The DPPH radical scavenging activity of the cookies significantly increased with increasing TCP. Based on the above results, this study suggested that TCP can be a good ingredient to increase the functionality of cookies.

• Journal of Nutrition and Health
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• 제43권2호
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• pp.105-113
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• 2010

천연 생리활성 물질로서의 흰씀바귀 뿌리의 특성을 검색하고자 성분분석, 세포 생존율, DPPH 라디칼 소거능을 검색하였다. 흰씀바귀 뿌리의 수분은 79.14%이었고 냉동건조시료 100g 당 조회분 2.49 g, 조지방 2.56 g, 조단백 8.28 g, 칼륨 216.95 mg, 인 89.15 mg이었고, 유리당은 glucose, sucrose 및 fructose으로 확인되었으며, 총 페놀 함량은 59.82 mg/100 g이었다. 지방산은 linoleic acid가 41.24%로 가장 많았고, 흰씀바귀 뿌리에서 확인된 아미노산은 총 17종으로 28.12 mg/g을 함유하였으며, 주요 아미노산 함량은 glutamic acid, arginine, aspartic acid 순으로 각각 5.54 mg/g, 5.03 mg/g, 2.96 mg/g이었다. 신장세포주인 Vero cell에서 메탄올 추출물과 각 분획별로 세포 생존율을 측정한 결과, 흰씀바귀 뿌리 메탄올 추출물과 부탄올 분획물, 물 추출물에서는 높은 세포 생존율로 독성이 거의 없었고 헥산, 클로로포름, 에틸아세테이트 분획물은 $100\;{\mu}g/mL$ 이상의 농도에서 낮은 세포 생존율을 보여 신장 세포의 독성이 관찰되었다. 특히 부탄올 분획물에서는 높은 세포 생존율을 나타내 신장 세포의 성장과 증식의 속도를 증가시키는 데 도움이 될 것으로 생각된다. 간 세포주인 chang cell에서도 같은 경향을 보였다. 흰씀바귀 뿌리 메탄올 추출물과 극성이 다른 5가지 용매, 헥산, 클로로포름, 에틸아세테이트, 부탄올, 물로 계통분획하여 DPPH 라디칼 소거능을 측정한 결과, 흰씀바귀 뿌리 메탄올 추출물 및 5가지 분획물의 DPPH 라디칼 소거능은 $4,000{\sim}0.12\;{\mu}g/mL$에서 농도 의존적으로 감소하였고, 50% 소거능 (${\mu}g/mL$)에 대해 에틸아세테이트 < 부탄올 < 클로로포름 < 헥산 분획물 < 메탄올 < 물 추출물의 순으로 나타났으며, 에틸아세테이트 분획물의 DPPH 라디칼 소거 효과가 가장 좋은 것으로 확인되었다. 본 연구에서 흰씀바귀 뿌리의 성분과 추출물 및 분획물의 세포 생존율과 DPPH 라디칼 소거능이 검토하였고 흰씀바귀에 대한 여러 다른 연구물들을 바탕으로 흰씀바귀가 건강기능성 식품의 신소재로 활용 할 수 있는 가능성을 확인하였다.