• Journal of the Korean Society of Food Science and Nutrition
• /
• v.45 no.3
• /
• pp.327-332
• /
• 2016

This study investigated the antioxidant and antimicrobial activities of various solvents (acetone, ethyl acetate, and ethanol) for extraction of Auricularia auricula-judae. Antioxidant activity was evaluated by determining total polyphenol and flavonoid contents, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation radical scavenging activity. Total polyphenol and flavonoid contents were not significantly different among the extracts, whereas DPPH radical scavenging activity and ABTS cation radical scavenging activity were significantly higher in ethanol and acetone extracts. DPPH radical scavenging activities of ethanol and acetone extracts showed high values (58.7% and 46.7%, respectively). The antimicrobial properties of these extracts were determined against six bacterial pathogens (Bacillus subtilis, Staphylococcus aureus, Micrococcus luteus, Escherichia coli, Pseudomonas aeruginosa, and Enterobacter cloacae) by the disc diffusion method. The acetone extracts showed antimicrobial activities against all tested bacteria, and all extracts showed the highest antimicrobial activity against B. subtilis.

• The Korean Journal of Food And Nutrition
• /
• v.26 no.4
• /
• pp.609-614
• /
• 2013

This study was carried out in order to investigate the functional properties of methanol extracts from two parts (root and fruit) of Xanthium strumarium by means of measuring the contents of total polyphenols and flavonoid as well as determining $ABTS{\cdot}^+$, DPPH radical scavenging activity, hydroxyl radical scavenging activity (OH${\cdot}$) and anticancer activity. The examination of physiologically active substances in the two parts revealed that the Xanthium strumarium fruit had high total polyphenol, flavonoid contents, $ABTS{\cdot}^+$ DPPH and hydroxyl radical scavenging activity. The Xanthium strumarium fruit has higher activities of anticancer activities on prostate cell lines compared to other cancer cell lines.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2018.04a
• /
• pp.87-87
• /
• 2018

매실나무겨우살이 막걸리의 생리적 효능을 검정하여 의약 화장품의 소재화 가능성을 검토할 목적으로 발효기간과 겨우살이 함량에 따른 총 폴리페놀, 플라보노이드 함량, DPPH free radical 소거능을 측정하였고, tyrosinase 저해활성은 5분 간격으로 65분간 측정하였다. 막걸리는 백미와 기장을 7:3의 비율로, 겨우살이의 함량을 0%, 5%, 10%로 달리하여 10일 간격으로 최대 30일까지 Shaking incubator($30{\sim}35^{\circ}C$, 100 rpm)에서 발효하였고, 발효가 끝난 막걸리는 80 mesh 망으로 여과하였다. 총 폴리페놀 함량은 10일에 최대치를 나타내었고, 0%는 $633.53{\mu}g/ml$, 5%는 $759.67{\mu}g/ml$, 10%는 $752.2{\mu}g/ml$을 나타내었다. 기간이 경과함에 따라 감소하는 경향이었으며 30일에 0%는 $507.57{\mu}g/ml$, 5%는 $570.13{\mu}g/ml$, 10%는 $551{\mu}g/ml$를 함유하고 있는 것으로 나타났다. 총 플라보노이드 함량 또한 10일에 최대치를 나타내었고, 0%는 $16.18{\mu}g/ml$, 5%는 $63.87{\mu}g/ml$, 10%는 $62.99{\mu}g/ml$였고, 기간이 경과함에 따라 감소하는 경향성을 나타내어 30일에 0%는 $0.59{\mu}g/ml$, 5%는 $10.98{\mu}g/ml$, 10%는 $1.18{\mu}g/ml$를 함유하였다. DPPH free radical 소거능은 0%의 경우 10일에 72.34%로 최대치가 나타났고, 30일에 58.61%로 가장 낮은 소거능을 보여주어 기간이 경과함에 따라 감소하는 경향성을 보여주었다. 하지만, 겨우살이 첨가군인 5%(10일:91.13%, 20일:91.09%)와 10%(10일:91%, 20일:90.8%)는 비교적 차이가 없었고, 발효기간이 30일이 경과한 후 5%는 93.46%, 10%는 92.3%로 최대값을 보여주었다. Tyrosinase 저해활성은 DPPH free radical 소거능이 가장 좋은 30일 발효 막걸리를 측정하였다. 모든 겨우살이 첨가군에서 5분이 경과하였을 때, 0%는 82.68%, 5%는 69.29%, 10%는 79.53%의 저해활성을 나타내었으며, 시간에 유의적으로 저해활성이 감소하였다. 본 연구 결과에 따르면, 겨우살이 막걸리의 총 폴리페놀과 플라보노이드 함량은 기간이 경과함에 따라 감소함에도 불구하고, DPPH free radical 소거능은 증가함을 알 수 있었다. Tyrosinase 저해활성은 5% 첨가 막걸리에서 가장 낮았으며, 겨우살이를 첨가하지 않은 0%에서 가장 저해활성이 높고 오랜시간 유지되는 것을 확인할 수 있었다.

• Korean Journal of Microbiology
• /
• v.45 no.4
• /
• pp.397-403
• /
• 2009

For the research of the natural antioxidant from marine sources, an antioxidant-producing marine actinomycetes was isolated from sea water in Jeju coastal area. The strain was identified based on 16S rDNA sequencing, the morphology by a method of scanning electron microscopy, physiological and biochemical characteristics and cellular fatty acid analysis. The isolated strain ACT-1 cell size was $0.5\sim1.0{\mu}m$ and gram positive, aerobic, nonmotile, substrate mycelium are red and gray aerial mycelium. 16S rRNA sequence analysis showed that were Gram-positive bacteria grouped on Streptomyces genus. Results of cellular fatty acid analysis showed that major cellular fatty acids were $C_{15:0}$ anteiso (39.33%), $C_{16:1}$ cis 9 (11.96%), $C_{16:0}$ (13.08%) and $C_{17:0}$ anteiso (10.99%). Finally, strain was identified Streptomyces sp. ACT-1. The antioxidant activity of methanol extract from Streptomyces sp. ACT-1 was evaluated by measuring DPPH, hydroxyl, and alkyl radical scavenging activity using an electron spin resonance (ESR) spectrometer. DPPH radical scavenging activity of SBME-1 (Streptomyces broth methanol extract) was 67% at $1,000{\mu}g$/ml. Hydroxyl radical scavenging activity of SBME-1 was 84% at $500{\mu}g$/ml. Alkyl radical scavenging activity of SBME-1 was 71% at $1,000{\mu}g$/ml.

• Journal of the Korean Applied Science and Technology
• /
• v.34 no.2
• /
• pp.296-304
• /
• 2017

The purpose of this study was to investigate the applicability of the Psidium guajava leaf extract as a whitening functional cosmetic material. We measured DPPH radical scavenging activity, intracellular ROS, cytotoxicity in B16F10 melanoma cells and cytoprotective effect on ultraviolet A, in vitro tyrosinase inhibitory effect and melanin biosynthesis inhibitory effect. The antioxidative effect was confirmed through high DPPH radical scavenging activity and intracellular ROS activity inhibition measurement of the Psidium guajava leaf extract. The survival rate of B16F10 melanoma cells was more than 98% at all concentrations, and the cytoprotective effect from ultraviolet ray A was found to increase in a concentration-dependent manner. In addition, in vitro tyrosinase activity inhibitory effect of 10% and melanin biosynthesis inhibitory effect of 20% were observed. Through less toxicity for B16F10 melanoma cell, high antioxidant activity, inhibition of tyrosinase activity and melanin biosynthesis inhibitory effect, we confirmed the possibility of developing the Psidium guajava leaf extract as a whitening functional cosmetic material with a safe and excellent whitening effect.

• KSBB Journal
• /
• v.18 no.6
• /
• pp.511-516
• /
• 2003

The methanol extract from leaves of Catalpa ovata 6. DoN showed DPPH (1,1-diphenyl-2-picrylhydrazyl) radical-scavenging activity, and its antioxidative compounds were studied. The ethyl acetate-soluble neutral fraction from the methanol extract was successively purified with silica gel adsorption column chromatography, Sephadex LH-20 column chromatography and HPLC. Three antioxidative compounds were isolated and identified as piperitol, pinoresinol and lariciresinol by HR-MS and NMR spectroscopic analyses. The DPPH radical-scavenging activity of the identified compounds decreased in the order of lariciresinol > pinoresinol > piperitol.

• The Journal of the Convergence on Culture Technology
• /
• v.8 no.2
• /
• pp.409-416
• /
• 2022

As a food ingredient, the extract has been proven of its antioxidant properties and safe to be consumed. It's increased antioxidant activity was evaluated by comparing its activity to the complex extract of fruits and vegetables, which is known to exhibit both antioxidant and physiological activities. In this experiment, after pre-heat treatment process, the increased antioxidant extract showed 41% of DPPH radical scavenging activity. The remarkable antioxidant activity of the produced extract will become useful in creating cosmetic or health products.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.38 no.12
• /
• pp.1649-1655
• /
• 2009

The antioxidant activities of water extracts from wild vegetables such as Ligularia fischeri (GC), Capsicum annuum L. (GCY), Aster scaber (CNM), Petasites japonicus S. et Z. Max (MYD), Ipomoea batatas L. (Lam) (GGM) were evaluated and compared with water extracts from freeze dried block. The antioxidant properties of water extracts from wild vegetables and their freeze dried block were evaluated using different antioxidant tests; 2.2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging, hydroxyl radical scavenging and nitrite scavenging activities. The water extracts from wild vegetables were found to have a higher total phenolic content than water extracts from freeze dried block. Total phenolic contents of water extracts from GC, GCY, CNM, MYD, and GGM were $471.66{\pm}3.52\;{\mu}g/mg,\;141.33{\pm}2.51\;{\mu}g/mg,\;177.33{\pm}2.88\;{\mu}g/mg,\;238.66{\pm}9.50\;{\mu}g/mg\;and\;122.67{\pm}3.51\;{\mu}g/mg$, respectively. At the concentrations of 1000 ppm, water extracts from GC, GCY, CNM, and GGM showed higher activities than water extracts from their freeze dried block on DPPH radical scavenger activity. The activity of water extracts from CNM, GC, GCY, MYD, and GGM were 90.9%, 89.9%, 76.6%, 71.1%, and 57.4%, respectively. When 10000 ppm of GC, GCY, CNM, MYD, and GGM water extracts tested for hydroxyl radical scavenging activity, activities were increased by 38.8%, 33.4%, 35.9%, 34.3%, and 33.8%, respectively and a similar effect was found with water extracts from GCY, CNM, and GGM freeze dried block at 10000 ppm concentration. However, the water extracts from GC and MYD was slightly more effective than freeze dried block extracts. The water extracts from wild vegetables and their freeze dried block had effective DPPH radical scavenger activity and hydroxyl radical scavenging activity at all tested concentrations. Nitrite scavenging activity of GC water extract significantly increased in a dose-dependent manner and the extract had higher nitrite scavenging activity than extracts freeze dried block extracts. We found that freeze dried block maintained antioxidant activities of the wild vegetables.

• Journal of Life Science
• /
• v.24 no.4
• /
• pp.386-392
• /
• 2014

Haw (Crataegus pinnatifida $B_{UNGE}$) is a medicinal plant commonly used in Korea due to its beneficial health effects. This study was performed to investigate the antioxidative activity of bioactive components from haw seed. Freeze-dried seed was processed with various solvents such as 70% methanol, 70% ethanol, a mixture of chloroform and methanol (2:1, v / v), n-butanol and ethyl acetate (EA). DPPH, reducing power, ferric reducing antioxidant power (FRAP), and ABTS radical scavenging activity decreased in this order: 70% methanol, 70% ethanol, EA, CM, n-butanol extracts. NO radical scavenging activity was high in the n-butanol extract (36.73% in 0.8 mg/ml concentration) and low in the 70% ethanol extract (24.96% in 0.8 mg/ml concentration). $OH^-$ radical scavenging activity of n-butanol extract (40.42% in 0.8 mg/ml concentration) was higher than the other extracts, but EA extract was the lowest (13.39%) at the same concentration. The results show that each extract contained various antioxidative substances and it seemed that different influence at each antioxidative activity.

• Journal of Applied Biological Chemistry
• /
• v.66
• /
• pp.503-511
• /
• 2023

The aim of this study was to investigate in vitro antioxidant activities of defatted Camellia japonica L. seeds (DCJS). The DCJS were extracted using ethanol and then fractionated with butanol (BuOH), ethyl acetate (EtOAc), chloroform, and hexane. To evaluate antioxidant activity of extract and fractions from DCJS, we investigated free radical scavenging activities such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS⁺), hydroxyl radical (^•OH), and superoxide anion (O₂^-) radicals. The five extract and fractions of DCJS dose-dependently increased DPPH, ABTS⁺ and O₂^- radical scavenging activities. The BuOH fraction of DCJS showed the highest free radical scavenging activities among other extract and fractions. The contents of total polyphenol and flavonoid in BuOH fraction of DCJS were 23.26 mg GAE/g and 32.39 mg QE/g, respectively. The polyphenol and flavonoids contents of BuOH fraction has highest than other extract and fractions. In addition, BuOH and EtOAc fraction of DCJS contained 102.37 and 165.05 ㎍/g of camelliaside B, respectively. Therefore, DCJS has higher antioxidant activity and may be useful as a natural antioxidant material.