• Korean Journal of Food Science and Technology
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• v.53 no.3
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• pp.290-295
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• 2021

This study investigated the antioxidant effects of complex extracts from pine needles (PN), green tea (GT), and sea buckthorn leaves (SL). Measurement of total polyphenol and flavonoid content, DPPH radical scavenging activity (DPPH), ABTS⁺ radical scavenging activity (ABTS), and reducing power (RP), showed that SL extract (SE) had the highest values. The superoxide radical scavenging activity (SSA) and nitrite scavenging activity (NSA) were the highest in the GT extract (GE). In most experiments, the PN extract (PE) showed higher activity in complex extracts than in single extracts. The combination of GT and SL led to a higher activity than that exhibited by GT (DPPH and ABTS at 300 ppm, RP at 100 and 500 ppm) and SL (SSA at 100 ppm and NSA at 300 ppm) alone. These results suggest that the combination of PE, GE, and SE may be a useful functional food material in the food industry.

• Korean Journal of Medicinal Crop Science
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• v.22 no.6
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• pp.442-448
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• 2014

The major objective of this study was to investigate the usability as cultivar for processing of new Citrus hybrid 'Hamilgam'. We investigated various antioxidant activities, such as DPPH radical, ABTS radical, Hydroxyl radical and Superoxide anion radical effect, along with total polyphenol content and total flavonoid content of Citrus hybrid 'Hamilgam', C. unshiu, C. natsudaidai. The total polyphenol content and total flavonoid content were the highest in the Hamilgam. The major flavonoids of Hamilgam and Natsudaidai were naringin and neohesperidin, and those of Unshu were narirutin and hesperidin, as determined by HPLC. Especially, the neohesperidin content of Hamilgam showed much higher (100 times over) than that of Unshu. However, the aglycone form was not found. DPPH and ABTS radical scavenging activities were the highest in Hamilgam peels. Hydroxyl radical scavenging activity was high in the order of Unshu, Hamilgam and Natsudaidai. Superoxide anion radical scavenging of 3 cultivars peels displayed low activities compared to DPPH and ABTS radical scavenging. Based on these results, Hamilgam peel extracts possess antioxidant activities and may thus serve as potential sources of functional food, cosmetic products, etc.

• Ocean and Polar Research
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• v.34 no.3
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• pp.297-304
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• 2012

Pylaiella littoralis was collected in the Chuuk lagoon of the Federated States of Micronesia (FSM). The FSM has a variety of coral reef ecosystems, which provide essential materials, such as minerals, vitamins, essential amino acids, for marine organisms. In this study, the antioxidant activities of ethanol and enzymatic extracts of P. littoralis were evaluated by measuring their scavenging activities on DPPH free radical, Alkyl radical, hydroxyl radical and cell viability. The enzymatic extracts were hydrolyzed to prepare water soluble extracts by using five carbohydrate degrading enzymes (AMG, Celluclast, Termamyl, Ultraflo, and Viscozyme) and five proteases (Alcalase, Flavourzyme, Kojizyme, Neutrase, and Protamex). As a result, the enzymatic extracts prepared by Flavourzyme, Ultraflo, and Kojizyme exhibited the greatest effects in DPPH free radical, alkyl radical scavenging activity and cell viability. Also, these enzymatic extracts had a higher antioxidant effect then commercial antioxidants in DPPH free radical and Alkyl radical scavenging activity. This study suggests that P. littoralis might be a useful source of natural antioxidants for the development of dietary supplements.

• Horticultural Science & Technology
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• v.30 no.2
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• pp.208-213
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• 2012

This study was performed to compare antioxidant activities of 80% EtOH extracts from young and mature fruit of three pear cultivars ('Wonhwang', 'Whangkeumbae', and 'Chuwhangbae') classified by three parts (peel, core, and flesh) of mature fruit. The total phenolics compound and flavonoid contents of 80% EtOH extracts from young fruit were 1.5-2.5 times higher than the mature fruit with great cultivar difference. In particular, the contents of those compounds were highest in 'Chuwhangbae' pear. The total phenolics compound and flavonoid contents of 80% EtOH extracts from mature fruit were the most in the peel, core, and flesh respectively. DPPH radical scavenging and $ABTS^+$ radical scavenging of 80% EtOH extracts from the young fruit were higher than the mature fruit of 'Chuwhangbae' pear. DPPH radical scavenging and ABTS+ radical scavenging of 80% EtOH extracts from mature fruit were the highest in the peel, core, and flesh respectively.

• FLOWER RESEARCH JOURNAL
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• v.17 no.4
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• pp.271-278
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• 2009

The attempts to develop natural antioxidants have been made with aerial part of 16 Compositae species by analyzing their phenolic compound contents, scavenging activities on 1,l-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radicals, ferrous ion chelating effects and inhibition effects on peroxidation of linoleic acids. Total polyphenol and flavonoid contents were highest in Matricaria recutica. Scavenging activity on DPPH and ABTS radicals were highest with extracts of Echinacea angustifolia and Serratula coronata var. insularis f. insularis, respectively. Aerial palt extracts of all species showed lower DPPH scavenging activity than ascorbic acid and 2,6-Di-tert-butyl-4-methylphenol (BHT). But Serratula coronata var. insularis f. insularis demonstrated higher ABTS scavenging activity than ascorbic acid and BHT. In Hieracium pilosella, Echinacea angustifolia, Matricaria reculica extracts showed higher ABTS scavenging activity than BHT. Ferrous ion chelating effects was highest with Matricaria recutica extract, but the effects were much lower than ethylenediaminetetraacetic acid (EDTA). The inhibition activity in lipid peroxidation of linoleic acids was highest in Eupatorium japonicum with 90.06% inhibition 4 days after reaction and 40.52% after 24 days. This demonstrated higher inhibition activity and longer lasting than BHT. Aerial part of Matricaria recutica for extraction source, rather than flower, has higher potential for antiox.idant material. In conclusion, development of natural antioxidants in Compositae is possible by studying antioxidant activity of each species.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.35 no.3
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• pp.251-256
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• 2009

Investigation of antioxidative constituents from the ethanol extract of Quercus gilva branches led to the identification of four compounds; catechin (1), epi-catechin (2), tyrosol (3) and tiliroside (4). The antioxidative activity was examined using DPPH radical and superoxide anion radical scavenging method. The isolated compounds 1, 2, 3 and 4 exhibited 94.2 %, 93.4 %, 33.6 % and 11.2 % scavenging activities respectively against DPPH radicals at the concentration of $100{\mu}L$/mL. As well, the compounds $1{\sim}4$ showed respectively 60.2 %, 35.1 %, 20.6 %, 4.5 % inhibition activities against superoxide anion radicals at $200{\mu}L$/mL. Interestingly, the compounds $1{\sim}4$ were isolated for the first time from Quercus gilva Blume.

• The Korean Journal of Food And Nutrition
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• v.35 no.5
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• pp.313-323
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• 2022

To investigate antioxidant effects of tea tree root extracts using various extraction methods, cytotoxicity, DPPH and ABTS radical scavenging, SOD, nitrite scavenging activity and inhibitory activity of lipid peroxidation, reducing power, ferrous ion chelating activity were measured. Cytotoxicity for RAW 264.7 cells was not observed at concentrations treated with below 90 ㎍/mL in all extracts. The maximum DPPH radical, nitrite scavenging, SOD activity and inhibitory activity of lipid peroxidation were obtained at the ethylacetate and 70% ethanol extract. The maximum ABTS radical scavenging activity was obtained at the ethylacetate and hot water extract. However, in the case of reducing power and ferrous ion chelating activity, they were obtained at 70% ethanol and hexane extract, respectively. Nitrate scavenging activity showed the most excellent scavenging ability of 59.6% at 90 ㎍/mL of ethylacetate. The hexane extract had the highest ferrous ion chelating activity, showing 61.05% at 50 ㎍/mL, 66.07% at 70 ㎍/mL and 76.81% at 90 ㎍/mL, respectively. The results of this research show that the ethylacetate and 70% ethanol extracts of tea tree root can be used as a natural material for scavenging the radicals. However, future study is necessary to understand the mechanism of antioxidant activity by identification of substances.

• Korean Journal of Plant Resources
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• v.21 no.4
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• pp.292-298
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• 2008

The antioxidant activities of Artemisia capillaris and Artemisia iwayomogi used as Injin (Korean herbal name) were investigated. The DPPH radical scavenging activity of the ethylacetate fractions obtained from the Artemisia capillaris and Artemisia iwayomogi was higher than other fractions. No DPPH radical scavenging activity was observed in hexane frcations. The nitrite scavenging activity was the highest at pH 1.2, and the peroxide value for linoleic acid was increased during the incubation.

• The Korea Journal of Herbology
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• v.32 no.6
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• pp.1-7
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• 2017

Objectives : The heartwood of Sappan Lignum has been used since ancient times as an ingredient in folk medicines against anti-bacterial and anti-anemia purposes. Many bioactive constituents have been derived from this biomass such as chalcones and homoisoflavonoids. In the current investigation, the antioxidant and anti-diabetic properties using DPPH and $ABTS^+$ radicals scavenging, ${\alpha}-glucosidase$, and advanced glycation end products (AGEs) inhibition assays were evaluated by different extraction methods of Sappan Lignum. Methods : In our continuing investigation for bioactive natural ingredients, the antioxidant and ${\alpha}-glucosidase$ inhibitory properties of Sappan Lignum extracts were prepared from different extraction methods and the biological efficacies were investigated in vitro. The antioxidant properties were evaluated employing radical scavenging assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) ($ABTS^+$) radicals. In addition, the anti-diabetic effects of Sappan Lignum extracts were tested via ${\alpha}-glucosidase$ and AGEs formation inhibitory assay. The total phenolic contents were determined using a spectrophotometric method. Results : All the tested samples showed dose-dependent radical scavenging and ${\alpha}-glucosidase$ inhibitory activities. Among the tested extracts, the 80% methanolic extract of Sappan Lignum was showed the most potent activity with an $IC_{50}$ value of $82.3{\pm}1.7{\mu}g/m{\ell}$ against DPPH radical scavenging assay. While, $ABTS^+$ radical scavenging activity of 80% methanolic extract was higher than those of other extracts. Also, ${\alpha}-glucosidase$ inhibitory and AGEs formation effects of each extacts and total phenolic contents were evaluated. Conclusions : These results suggested that Sappan Lignum can be considered as a new effective source of natural antioxidant and anti-diabetic materials.

• Food Science of Animal Resources
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• v.25 no.3
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• pp.344-349
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• 2005

Two different NokJoongTangs were prepared by boiling antler and various oriental medicinal materials. The phenolic contents and free radical scavenging effects against DPPH(1,1-diphenyl-2-picrylhydrazyl) radical and hydroxyl radical of the NokJoongTangs which are used as the health supplement foods, were investigated. NokJoongTang-II prepared dried antler with various oriental medicinal materials, had higher phenolic content than that of NokJoongTang-I prepared 1mm raw antler with the same materials. In addition, NokJoongTang-II exhibited higher scavenging activities than these of NokJoongTang-I against DPPH radical and hydroxyl radical. These result indicate that free radical scavenging activity of NokJoongTang showed a difference by using raw and dry antler for NokJoongTang preparation, and that the findings also show a positive correlation between radical scavenging activity and total phenolic contents.