• 생약학회지
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• 제35권1호통권136호
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• pp.80-87
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• 2004

From the $CHCl_3$ and BuOH soluble fractions of the heartwood of Hemiptelea davidii, eleven compounds have been isolated. On the basis of spectral data, they were identified as ${\beta}-sitosterol$ (1), scopoletin (2), kaempferol (3), 4-hydroxybenzoic acid (4), 2-(4-hydroxyphenyl) ethanol (5), aromadendrin (6), scopolin (7), kaempferol 6-C-glucoside (8), aromadendrin 6-C-glucoside (9), taxifolin 6-C-glucoside (10) and quercetin 6-C-glucoside (11), respectively. Among these compounds, compounds 3, 8, 10, and 11 showed potent DPPH radical scavenging activity with $IC_{50}$ values of 11.9, 14.7, 10.3 and $6.2\;{\mu}g/ml$, respectively.

• Food Science and Biotechnology
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• 제15권1호
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• pp.39-43
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• 2006

Levels of quercetin in different parts of onion were investigated using high performance liquid chromatography (HPLC) and liquid chromatography mass spectrometry (LC/MS) suitable for use with functional food material. Two main peaks were observed on HPLC chromatograms from the extracts of the skin, and the outer, middle, and core parts of onion. Using LC/MS, peak 1 was tentatively identified as quercetin monoglucoside at m/z 466.4, and peak 2 as quercetin with [M]+ at m/z 303.3. The levels of quercetin in the skin, and the outer, middle and core parts of the plant were 16.83,2.67,0.95, and 0.35 mg/g, respectively. In the study of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, skin, the nonedible part, contained the highest amount of quercetin, compared to the other edible parts, and showed the highest DPPH radical scavenging activity. Levels of quercetin and DPPH radical scavenging activity increased from core to skin. All parts of onion exhibited the strongest antibacterial activity only against Staphylococcus aureus and Vibro parahaemolyticus. Antibacterial activities of onion exhibited that S. aureus was more sensitive than V. parahaemolyticus. Among the four onion extracts, the middle part showed the strongest inhibitory activity against S. aureus but all onion extracts showed similar antibacterial activities against V. parahaemolyticus.

• 미생물학회지
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• 제45권1호
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• pp.54-57
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• 2009

대두발효식품인 청국장에는 다양한 항산화물질, peptide 등의 생리활성물질이 존재한다. 청국장 ethanol 추출물은 285 nm에서 0.55의 흡광도 값을 보여 주었다. 이 영역에는 phenol을 포함한 아미노산 및 peptide류가 포함되어 있다고 알려져 있다. 1,1-diphenyl-2-picrylhydrazyl (DPPH)방법으로 청국장의 항산화도를 측정했을 때, ethanol 추출물의 농도가 증가할수록 항산화도는 증가하였다. 무염 생청국장 30 g을 복용하고 2시간 마다 혈압을 측정하였다. 수축기혈압은 복용 후 6시간 지난 후, 평균혈압이 14 mmHg 떨어졌고, 이완기혈압은 8 mmHg 떨어지는 등 혈압강하 효과가 뚜렷하였다. 청국장에 존재하는 daidzein, 항산화물질, Lys-Pro과 같은 angiotensin I-converting enzyme (ACE) 억제제 등이 복합적으로 작용하면서 혈압강하에 기여할 수 있을 것이다.

• Food Science and Biotechnology
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• 제18권6호
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• pp.1464-1469
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• 2009

Jiwhang (Rehmannia glutinosa), one of the most widely used medicinal herbs, was dried with various methods such as sun drying, hot air drying, vacuum drying, and freeze drying methods, and their effects on the antioxidant capacity in relation with the content of total phenolic compounds were studied with a steamed-and-dried rehmannia (sookjiwhang) for comparison. Generally, total phenolic contents decreased significantly by all of the drying treatments except the steamed-and-dried rehmannia, in which total phenolic contents increased 2.4 fold compared with fresh rehmannia. Content of verbascoside, a functional phenolic compound, was the highest in the freeze-dried rehmannia ($177.97{\pm}0.02\;{\mu}g/g$ d.m.) followed by vacuum-dried ($105.55{\pm}0.07\;{\mu}g/g$ d.m.), hot air-dried ($23.01{\pm}0.02\;{\mu}g/g$ d.m.), and sun-dried ($4.89{\pm}0.13\;{\mu}g/g$ d.m.) ones comparable to the fresh rehmannia ($80.15{\pm}1.26\;{\mu}g/g$ d.m.). Antioxidant capacity determined by both 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethyl-benzthiazoline-6-sulphonic acid) (ABTS) methods agreed with the result of total phenolic contents, that is, the antioxidant capacity was the highest in the steamed-and-dried rehmannia followed by fresh rehmannia, vacuum-dried, hot air-dried, sun-dried, and freeze-dried ones. Conclusively, the total phenolic contents and antioxidant capacity of rehmannia were greatly affected by the drying methods used.

• Natural Product Sciences
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• 제20권1호
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• pp.22-28
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• 2014

A new natural product, tetrastigmol A (1), and several known compounds as flavonoids (2 - 8), steroids (9 - 10), bergenin and its derivative (11 - 12), stilbens (13 - 15), lignan (16), benzenecarboxylic derivative (17) and two norisoprenoid (18 - 19) were isolated from the stem of Tetrastigma erubescens Planch. (Vitateae). Their structures were determined on the basis of NMR spectroscopic data. This is the first report on chemical constituents of this plant. Compounds 1, 6 - 8 and 12 - 15 showed strong antioxidant activity using two methods including DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging and lipid peroxidation inhibitory assays.

• 한국응용과학기술학회지
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• 제34권2호
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• pp.271-279
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• 2017

본 연구는 황련으로부터 분리된 fraction의 항균효능과 항산화 효과를 평가하고 그것의 화장품 소재로서의 가능성을 확인하였다. 황련으로부터 분리된 fraction의 항균활성은 Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Candida albicans 균주로 disc diffusion 방법을 통해 생육저해환(clear zone)을 측정하였다. 그 결과 Fr. 1을 제외한 모든 시료에서 S. aureus와 candida. A에서 항균활성을 나타내는 것으로 확인 하였다. 항산화 평가를 위해 황련 fraction의 농도(50, 125, 250) ${\mu}g/mL$에 따라 처리하여 1,1-diphenyl-2-picrylhydrazyl (DPPH) 라디칼 소거능과 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) 양이온 라디칼 소거능을 확인하였다. 그 결과 Fr. 1, 2, 3, 4의 $250{\mu}g/mL$ 농도에서 DPPH 라디칼 소거능 활성이 각 11.4%, 30.3%, 42.0%, 53.1%로 $ABTS^+$ 라디칼 소거능 활성은 동일농도에서 각 28.6%, 96.2%, 98.6%, 97.1%로 나타났다. Fr. 3, 4는 동일농도의 대조군 BHT 활성의 86.5%보다 높은 활성산소 저해능을 보였다. 황련의 세포독성을 측정한 WST assay 결과에서 Fr. 4를 제외하고는 Fr. 1, 2, 3은 독성을 나타내지 않았다. 이러한 결과로 황련으로부터 분리된 fraction은 항균능과 항산화 능을 가지는 화장품 소재로서의 가치를 가진다고 볼 수 있다.

• 대한화장품학회지
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• 제44권3호
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• pp.317-325
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• 2018

본 연구는 갯버들 추출물의 항산화 활성 및 미백활성을 평가하기 위해 수행하였다. 갯버들 줄기의 용매별 추출물의 총 폴리페놀 함량은 142.60-151.95 mg GAE/g, 총 플라보노이드 함량은 83.43-2.60 mg CE/g으로 확인되었다. 갯버들 줄기의 용매별 추출물의 1,1-diphenyl-2-picrylhydrazyl (DPPH) 라디칼 소거활성을 평가한 결과 30% 메탄올 추출물이 가장 높은 항산화 활성($IC_{50}$; $19.68{\mu}g/mL$)을 나타내었다. Tyrosinase 억제활성은 4종류의 용매 추출물 모두 유사하게 평가되었으며, 30% 에탄올 추출물 $200{\mu}g/mL$ 농도가 가장 높은 억제활성(35.18%)을 나타내었다. 갯버들 추출물 중 메탄올 추출물은 B16F10 세포주의 생존율에 영향을 미치지 않은 농도에서 유의적으로 멜라닌 생합성을 저해하는 것으로 확인되었다. 결과적으로 갯버들 추출물은 항산화 및 미백활성을 가지는 효과적인 화장품 성분으로 사용 가능할 것으로 사료된다.

• 한국식품저장유통학회지
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• 제30권6호
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• pp.960-968
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• 2023

The rhizome of Phragmites communis Trin. is used for vomiting and belching by clearing stomach and the sprout is used as tea. Phragmites japonica is similar with P. communis except the color of sheath is purple. This study is aimed to compare the in vitro antioxidant activity, total polyphenol and flavonoids contents of P. communis and P. japonica. The antioxidant activities of fractions from the two Phragmites plants were evaluated by 1,1-diphenyl-2- picrylhydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assay. The antioxidant activity varied with plant parts and extract solvents. The fractions of leaf extract from the two Phragmites plants (4.06±1.32-16.47±1.28%) showed higher antioxidant activity by DPPH assay compared with rhizome fractions of two Phragmites plants (0.00±0.00-14.15±0.07%), these are lower compared with ascorbic acid and butylhydroxyanisole (BHA). The highest ABTS radical scavenging activity was found for rhizome ether fraction, namely 74.95±0.56% and 73.04±1.85% for P. communis and P. japonica, these are higher than BHA. The total polyphenol and flavonoids contents were different with plant parts and extract solvents, likewise antioxidant activity. A significant correlation was shown between DPPH and ABTS radical scavenging activity. Considering the results of this study, the leaves and stems of P. communis and P. japonica are expected to be used as natural antioxidants.

• Preventive Nutrition and Food Science
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• 제16권4호
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• pp.328-332
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• 2011

After preparation of acetone, ethanol, methanol, and water extracts (10 g/300 mL) of dried persimmon (Diospyros kaki cv. Fuyu) flower-buds, total phenolic contents (TPC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (RSA), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) RSA, reducing power (RP), and tyrosinase inhibitory activity of the extracts were evaluated. The methanol extracts produced the highest TPC (113.39 mg gallic acid equivalents/g), DPPH RSA ($IC_{50}=40.25\;{\mu}g/mL$), ABTS RSA ($IC_{50}=58.17\;{\mu}g/mL$) and RP ($IC_{50}=69.43\;{\mu}g/mL$) activities while the water extracts generated the lowest values. The ethanol extract showed the highest tyrosinase inhibitor activity (88.90%) at a concentration of 1 mg/mL. These results indicated that persimmon flower-buds may be a useful source of natural antioxidants.

• 한국식품영양학회지
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• 제24권4호
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• pp.713-719
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• 2011

The antioxidant activities of the ethanol extract of Arctium lappa were assessed by measuring the 1,1-diphenyl-2-picrylhydrazyl( DPPH) radical scavenging effect, inhibition of $Fe^{2+}$-induced lipid peroxidation, inhibition of malondialdehyde(MDA)-bovine serum albumin(BSA) conjugation reaction and antimutagenic capacities using the Ames test. The DPPH radical scavenging activity and inhibition of $Fe^{2+}$-induced lipid peroxidation of the $Arctium$ $lappa$ ethanol extract significantly increased in a dose-dependent manner. In the radical scavenging assay using DPPH, the $IC_{50}$ of the Arctium lappa extract was 296 ${\mu}g$/assay(1.29 mg of dry sample). In addition, the $IC_{50}$ in the inhibition of $Fe^{2+}$-induced lipid peroxidation was 1,759 ${\mu}g$/assay(7.65 mg of dry sample). This extract also significantly inhibited the MDA-BSA conjugation reaction with an $IC_{50}$ of 57.58 mg/assay(250 mg of dry sample). However, no inhibitory effects against the direct and indirect mutagenicities in $Salmonella$ Typhimurium TA98 and TA100 were observed. Based on these results, the ethanol extract of $Arctium$ $lappa$ was shown to display considerable antioxidative activities.