Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Studies on Antioxidant and Whitening Activities of Salix gracilistyla Extracts

갯버들 추출물의 항산화 및 미백활성 연구

  • Jeong, Yong-Un (Department of Integrated Biosciences, Research Institute for Biomedical & Health Science, College of Biomedical and Health Science, Konkuk University) ;
  • Park, Young-Jin (Department of Integrated Biosciences, Research Institute for Biomedical & Health Science, College of Biomedical and Health Science, Konkuk University)
  • 정용운 (건국대학교 의료생명대학 바이오융합과학부, 의료생명연구소) ;
  • 박영진 (건국대학교 의료생명대학 바이오융합과학부, 의료생명연구소)
  • Received : 2018.08.03
  • Accepted : 2018.09.18
  • Published : 2018.09.30
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Abstract

This study was carried out to evaluate the antioxidant and whitening activities of Salix gracilistyla extracts. The total polyphenol contents of the extracts were 142.60-151.95 mg GAE/g and total flavonoid contents were 83.43-92.60 mg CE/g. In 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, the 30% methanol extract showed the highest antioxidant activity ($IC_{50}$; $19.68{\mu}g/mL$). Tyrosinase inhibitory activity was similar in all four solvent extracts, and the highest inhibitory activity (35.18%) was obtained at a concentration of $200{\mu}g/mL$ of 30% ethanol extract. In addition, methanol extracts did not affect cell viability at all treatment concentrations and were found to significantly reduce the melanin content of B16F10 cells. As a result, it is considered that Salix gracilistyla extracts can be used as an effective cosmetic ingredient having antioxidant and whitening activity.

본 연구는 갯버들 추출물의 항산화 활성 및 미백활성을 평가하기 위해 수행하였다. 갯버들 줄기의 용매별 추출물의 총 폴리페놀 함량은 142.60-151.95 mg GAE/g, 총 플라보노이드 함량은 83.43-2.60 mg CE/g으로 확인되었다. 갯버들 줄기의 용매별 추출물의 1,1-diphenyl-2-picrylhydrazyl (DPPH) 라디칼 소거활성을 평가한 결과 30% 메탄올 추출물이 가장 높은 항산화 활성($IC_{50}$; $19.68{\mu}g/mL$)을 나타내었다. Tyrosinase 억제활성은 4종류의 용매 추출물 모두 유사하게 평가되었으며, 30% 에탄올 추출물 $200{\mu}g/mL$ 농도가 가장 높은 억제활성(35.18%)을 나타내었다. 갯버들 추출물 중 메탄올 추출물은 B16F10 세포주의 생존율에 영향을 미치지 않은 농도에서 유의적으로 멜라닌 생합성을 저해하는 것으로 확인되었다. 결과적으로 갯버들 추출물은 항산화 및 미백활성을 가지는 효과적인 화장품 성분으로 사용 가능할 것으로 사료된다.

Keywords

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