• 한국자기공명학회논문지
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• 제21권1호
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• pp.13-19
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• 2017

Human sterol ${\Delta}8-{\Delta}7$ isomerase, commonly known as emopamil binding protein (EBP), is an essential protein in the cholesterol-synthetic pathway, and mutations of this protein are critically associated with human diseases such as Conradi-Hunermann-Happle or male EBP disorder with neurological defects syndrome. Due to such a clinical importance, EBP has been intensively investigated and some important features have been reported. EBP is a tetra-spanning membrane protein, of which $2^{nd}$, $3^{rd}$, and $4^{th}$ membrane-spanning ${\alpha}$ helices play an important role in its enzymatic function. However, detailed structural feature at atomic resolution has not yet been elucidated, due to characteristic difficulties in dealing with membrane protein. Here, we over-expressed EBP using Escherichia coli and performed detergent screening to find suitable membrane mimetics for structural studies of the protein by NMR. As results, DPC and LMPG could be evaluated as the most favorable detergents to acquire promising NMR spectra for structural study of EBP.

• 한국자기공명학회논문지
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• 제7권1호
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• pp.37-45
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• 2003

Human CD99 is a ubiquitous 32-kDa transmembrane protein encoded by mic2 gene. Recently it has been reported that expression of a splice variant of CD99 transmembrane protein (Type I and Type II) increases invasive ability of human breast cancer cells. To understand structural basis for cellular functions of CD99 Type II, we have initiated studies on hCD99$\^$TMcytoI/ using circular dichroism (CD) and multi-dimensional NMR spectroscopy. CD spectrum of hCD99$\^$TMytoI/ in the presence of 200mM DPC and CHAPS displayed an existence ${\alpha}$-helical conformation, showing that it could form an ${\alpha}$-helix under membrane environments. In addition, we have found that the cytoplasmic domain of CD99 would form symmetric dimmer in the presence of transmembrane domain. Although it has been rarely figured out the correlation between structure and functional mechanism of hCD99$\^$TMcytoI/, the dimerization or oligomerization would play an important role in its biological function.

• 한국자기공명학회논문지
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• 제21권3호
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• pp.78-84
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• 2017

Pseudin is a naturally occurring 24 amino-acid-residue antimicrobial peptide derived from the skin of paradoxical frog Pseud's paradoxa. It shows potency against the bacteria and antibiotic-resistant bacteria strain, but has high cytotoxicity against mammalian cell. In our previous study, substitution of $Pro^{11}$ for Gly (Ps-P) increased bacterial cell selectivity but decreased the antibacterial activity of pseudin. In this study, we designed pseudin analogue, Ps-4K-P with increased cationicity up to +7 in Ps-P by substituting Glu14, Gln10, Gln24, and Leu18 with Lys. Ps-4K-P showed improved potent antibacterial activity with high bacterial cell selectivity. We determined the tertiary structure of Ps-4K-P in the presence of DPC micelles by NMR spectroscopy and it has a hinge structure at $Pro^{11}$ followed by three turn helices from $Pro^{11}$ to $Val^{23}$ at the C-terminus. Amphipathicity with increased cationicity as well as helix-hinge-helix structural motif provided by introduction of a Pro at position $Gly^{11}$ are the crucial factors which confer antibacterial activity with bacterial cell selectivity to Ps-4K-P.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2002년도 추계학술발표논문집 (하)
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• pp.2341-2344
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• 2002

우편물량 처리에서 병목현상의 원인이 되고있는 수작업에 의한 순로구분 작업을 원활하고 신속하게 처리하며 집배원의 업무 부담을 경감시키기 위해 자동화가 시도되고 있다. 이러한 자동화된 순로구분기의 개발에 있어 순로구분에 필요한 주소 데이터 베이스를 관리하기 위해서는 각 배달지점에 대한 고유한 ID 부여가 필요하다. 따라서 본 논문에서는 아직 체계화되지 않은 한국 주소의 배달점 코드 설계 방안에 대해서 연구하였다. 먼저 해외 각국의 배달점 코드할당 방법을 알아보고, 배달점 코드의 기본 정의 및 설계 방향을 제시한다. 그리고 각 주소의 고유한 배달점 코드를 설계하는데 있어 여러 가지 방안을 제시하고 각 대안별 장단점 및 설계 방법을 제시하였으며 적합한 설계 방안을 선정하였다. 또한 기존의 주소체계뿐만 아니라 도입 예정인 새주소 체계와의 적합성도 고려하였다. 본 논문에서 제시한 우편번호 앞 3자리와 6자리의 일련번호를 연결한 배달점 코드가 현재의 한국 주소체계 뿐만 아니라 새주소 체계에도 적합할 것으로 판단된다. 마지막으로 몇 가지 적용 사례를 통한 제안한 방안들을 검증해 보았다.

• International Journal of Industrial Entomology and Biomaterials
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• 제7권1호
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• pp.21-27
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• 2003

Manifestation of heterotic genetic interaction was studied in different hybrids made between multivoltine recurrent backcross (RBL)/congenic lines (Con. L) during unfavourable season when temperature and relative humidity are > $30^{\circ}C$ and 86%, respectively. A few number of silkworm race or strain or breed like Nistari (N ＋ p or Np) can sustain the temperature above 3$0^{\circ}C$ and RH above 86%. The present heterosis study screened a hybrid i.e., CB$_{5}$Lm5RBL1M$_{6}$DPC-LmE$^1$RBL and its reciprocal provided heterobeltiotic effect on survival by number and pupation rate at a magnitude of 20% (p < 0.01) and yield by weight of 10% (p < 0.01). Beside all the hybrids expressed heterosis over check - Nistari (N ＋ p) with better quality silk. Therefore, aforesaid hybrid may be useful for utilization at commercial level during adverse seasons of West Bengal.gal.

• 한국병원경영학회지
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• 제13권4호
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• pp.101-118
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• 2008

Japanese national health expenditure was 8.0% of GDP in 2004, and it was lower than average of OECD countries. But it has increased rapidly in recent years. Japan has relatively many acute care beds and high-price medical equipments, and the average length of stay is long. Japanese government is trying healthcare reform to contain healthcare expenditure, increase the efficiency of management and improve the quality of healthcare. As healthcare policies for hospitals such as DPC (Diagnosis Procedure Combination) for acute care beds, reduction of long-term care beds, and functional differentiation and liaison among healthcare institutions are implemented, the number of hospitals in financial difficulties is increasing. The serious situation urges hospitals to adapt to changes and search new directions of management. They need to establish and implement appropriate positioning strategy, and increase management efficiencies. Korean healthcare system has similarities with Japanese in many aspects. The recent reform and changes in Japanese healthcare system and hospitals give suggestions to Korean hospitals as to how they can prepare for environmental changes and improve management.

• 한국환경성돌연변이발암원학회지
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• 제19권1호
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• pp.20-27
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• 1999

In order to know the inhibitory effect of magnesium carbonate(MgCO3) on cytotoxicity, DNA damage, mutagenicity, and cell transforming ability of nickel subsulfide, the inhibition of cell proliferation, DNA-protein crosslinks formation (DPC), HGPRT point mutation, and cell transformation were evaluated. Nickel subsulfide(Ni3S2) and magnesium carbonate as insoluble compounds were used for this study. BALB/3T3 cell, CHO-K1 cell, and C3H10T1/2 cell were used in this experiment. Exposure concentration of nickel subsulfide was 1 $\mu\textrm{g}$/ml. The concentrations of magnesium carbonate in this study were 0.6 $\mu\textrm{g}$/ml, 1.2 $\mu\textrm{g}$/ml, 2.4 $\mu\textrm{g}$/ml and the molar ratio of magnesium to nickel when exposed simultanously were 0.5, 1.0 and 2.0 respectively. The results were as follows; 1. Magnesium carbonate reduced the inhibitory effect of nickel subsulfide on cell proliferation. 2. Magnesium carbonate also reduced the effect of nickel subsulfide on DNA-protein crosslinks formation. 3. HGPRT point mutagenicity of nickel subsulfide was reduced when magnesium carbonate treated simultaneously. 4. Magnesium carbonate reduced cell transforming ability of nickel subsulfide. Conclusively, nickel subsulfide showed cytotoxicity, cell transforming ability, and mutagenicity strongly and magnesium carbonate may have protective roles in these nickel effects.

• 한국식물병리학회지
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• 제13권1호
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• pp.37-45
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• 1997

Disease incidence (DI), pre-emergence damping-off (PDO), days until the first symptom appeared (DUS), disease progress curve (DPC), and area under disease progress curve (AUDPC) were investigated in vivo after sowing ginseng seeds in each of 37 ginseng-cultivated soils which were sampled from 4 regions in Korea. Non linear fitting parameters, A, B, K and M, were estimated from the Richards' function, one of the disease progress models, by using the DI at each day from the bioassay. Inter- and intra-relationships between disease variables and stand-missing rate (SMR) in fields were investigated by using the simple correlation analysis. Disease variables of the root rot were divided into two groups: variables related to disease incidence, e.g., DI, AUDPC and A parameter, and variables related to disease progress, e.g., B, K and M parameters. DI, AUDPC, and DUS had significant correlations with SMR in ginseng fields, and then it showed that the disease development in vivo corresponded with that in fields. Soil samples could be separated into 3 and 4 groups, respectively, on the basis of the principal component 1 (PC1) and the principal component 2 (PC2), which were derived from the principal component analysis (PCA) of Richards' parameters, A, B, K and M. PC1 accounted for B, K and M parameters, and PC2 accounted for A parameter.

• Asian Pacific Journal of Cancer Prevention
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• 제15권24호
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• pp.10971-10975
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• 2015

Objective: To investigate bladder and intestinal function recovery and quality of sexual life after laparoscopic nerve-sparing radical hysterectomy (LNRH) for treatment of early invasive cervical carcinoma. Methods: Subjects included patients who underwent radical hysterectomy by laparotomy who were randomly assigned to 2 groups: 30 patients who underwent LNRH and 35 classical laparoscopic radical hysterectomy (LRH). We assessed the patients general clinical information, surgical characteristics, pathological findings, and adjuvant therapies. A urodynamic study was used to assess bladder function. Intestinal function recovery and quality of sexual life were evaluated by questionnaire. Results: No significant differences were found in age, surgery characteristics, pathological findings, adjuvant therapies, and main adverse effects between the 2 groups. The mean duration of the postoperative catheterization (DPC) in group LNRH was shorter than that in group LRH (P < 0.001). The maximum flow rate, maximum cystometric capacity, maximum detrusor pressure and urinary complications in group LNRH were better than those in group LRH. The quality of sexual life evaluated according to the female sexual function index (FSFI) was better in group LNRH than in those who underwent LRH. The intestinal function of patients in group LNRH also recovered better compared with patients in group LRH.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제7권9호
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• pp.2120-2133
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• 2013

In this paper, we address the problem of linear minimum mean-squared error (MMSE) transmitter design for the cognitive radio (CR) multi-user multiple-input single-output (MU-MISO) broadcasting channel (BC), where the cognitive users are subject to not only a sum power constraint, but also a interference power constraint. Evidently, this multi-constraint problem renders it difficult to solve. To overcome this difficulty, we firstly transform it into its equivalent formulation with a single constraint. Then by utilizing BC-MAC duality, the problem of BC transmitter design can be solved by focusing on a dual MAC problem, which is easier to deal with due to its convexity property. Finally we propose an efficient two-level iterative algorithm to search the optimal solution. Our simulation results are provided to corroborate the effectiveness of the proposed algorithm and show that this proposed CR MMSE-based scheme achieves a suboptimal sum-rate performance compared to the optimal DPC-based algorithm with less computational complexity.