• Title/Summary/Keyword: DOG1 protein

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Electrophoretic analysis of the major protein of erythrocyte membrane in man, bovine, horse, and dog: their relation to erythrocyte sedimentation rate (사람, 소, 말, 개의 적혈구막 단백의 전기 영동법에 의한 분석 - 적혈구 침강 속도와의 관계 -)

  • Bahk, Yeong-woo
    • Korean Journal of Veterinary Research
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    • v.41 no.1
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    • pp.21-28
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    • 2001
  • The protein of the bovine, horse and dog erythrocyte membrane were analyzed by polyacrylamide gel eletrophoresis in sodium dodecyl sulfate and their relation to the sedimentation rate of animal erythrocytes were investigated by treating the erythrocytes with proteinases such as trypsin and chymotrypsin. Protein content in erythrocyte membrane was in human, in Jindo dog, in cattle and in horse, showing similar in among. The erythrocyte sedimentation rates bovine erythrocytes from Hostein and Korean native cattle were very slow compared with the human one(1/7 as slow as the human one) as reported previously. Although the general protein profiles of the bovine erythrocyte membranes were almost similar to that of human, bovine erythrocyte membranes showed one additional protein band, called band Q in this study, which migrated electrophoretically to the mid-position between band 2 and band 3 in human erythrocyte membranes. The erythrocyte sedimentation of race horse were very fast compared with the human one are reported previously. Although the general protein profiles of the race horse erythrocyte membranes were almost similar to that of human, band 3 content was showing higher in race horse(34.7%) than in human(25.3%). The general protein profile of the Jindo dog erythrocyte membrane was almost similar to the human patterns, Jindo dog erythrocyte membranes showed one absent protein band. It was band 7. The glycoprotein profiles of the bovine erythrocyte membranes revealed by periodic acid-Schiff(PAS) stain showed a marked difference from that of human. The PAS-1(glycophorin) and PAS-2(sialoglycoprotein) present in human erythrocyte membrane were almost absent from the bovine erythrocyte membranes showed a strong PAS-positive band near the origin of the electraphorograms, which is named as PAS-B in this study. The PAS-1 and PAS-2 present in human erythrocyte membrane were almost absent from race horse erythrocyte membranes, but PAS-2 was more in only race horse from that of human. The PAS-1 and PAS-2 were absolutely absent from the Jindo dog erythrocyte membrane. These results suggest the slow sedimentation rate of bovine erythrocytes is due in part to the presence of band Q protein fraction and PAS-B glycoprotein in the bovine erythrocytes, and that the fast sedimentation rate of race horse erythrocyte is due in part to the presence of more band 3 protein fraction and PAS-E glycoproteins in the race horse erythrocytes.

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Changes in Edible Culture of Dog Meat and Evolutionary Study (식용견 문화의 변화와 진화론적 고찰)

  • Sim, Soon-Chul;Choi, Hyun-Jung
    • Culinary science and hospitality research
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    • v.24 no.1
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    • pp.122-129
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    • 2018
  • The purpose of this study is to understand the evolution of food culture by applying the evolutionary mechanism to the process of forming the dog meat culture. To do this, this study first examined mutation, selection, and replication as a evolutionary mechanism by biological genes and explain the evolutionary process of food culture by applying so-called 'mime' which is a virally-transmitted cultural symbol or social idea. A meme acts as a unit for carrying cultural ideas, practices, that can be transmitted from one mind to another through writing, speech, gestures, rituals, or other imitable phenomena with a mimicked theme. In addition, this study also intended to use in-depth interviews on how people have diverse cultural perspectives interpret and accept edible culture of dog meat. In Korea, which was a traditional farming society, dog meat which is easier to obtain compare to beef has been chosen as an important source of protein. And this choice has been repeatedly reproduced through generations. However, the current generation's awareness of the edible culture of dog meat has changed. The meme of pet culture has been selected and replicated, and this cultural evolution will eventually lead to the culling of dog meat.

Effects of Crude Protein Levels on Nitrogen Balance in Adult Jindo Dog (사료내 조단백질 수준이 진도성견의 질소균형에 미치는 영향)

  • Kang, Tae-Il;Maeng, Won-Jai;Kim, Myeong-Hwa;Lee, Sang-Rak
    • Journal of Animal Science and Technology
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    • v.51 no.2
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    • pp.155-162
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    • 2009
  • This study was conducted to determine the protein requirement level in adult Jindo dog (Korea Jindo Dog) through nitrogen balance experiments. 12 female dogs aged 36~38 weeks old (for early stage) or aged 45~49 weeks old (for late stage) were fed one of 3 diets containing 17, 19 and 21% of crude protein. Body weight of dogs were measured during the 12 days of feeding trial, and feces and urine were collected last 3 days of the trial period. In early stage of Jindo dog, average daily gain of dogs fed experimental diets containing 17, 19 and 21% of crude protein were -38.33, -16.25 and -12.71g/d, respectively. There was a significant differences between 17 and 21% of crude protein treatments (p<0.05) and calculated retained nitrogen were -0.29, -0.04 and -0.03g/kg$BW^{.75}$/d, respectively. In late stage of Jindo dog, average daily gain of dogs fed experimental diets containing 17, 19 and 21% of crude protein were -34.05, -28.71 and -28.28g/d, and calculated retained nitrogen were -0.33, -0.06 and 0.09g/kg$BW^{.75}$/d, respectively, and were significantly higher (p<0.05) in dogs fed 21% of crude protein diet than those of other dogs. In early stage and late stage of adult Jindo dog, a calculated linear regression equation for nitrogen intake (x) and nitrogen retention (y) were y = 0.7484x-1.18 ($R^2$ = 0.9923) and y = 0.88231x-1.0894 ($R^2$ = 0.9982) and the requirement of crude protein were estimated as 9.85g/kg$BW^{.75}$/d and 7.72g/kg$BW^{.75}$/d from above equation.

Effects of Protein Levels on Growth and Nitrogen Balance in Growing Jindo Dog (단백질 수준이 진도자견의 성장과 질소평형에 미치는 영향)

  • Kang, T.I.;Maeng, W.J.;Kim, M.H.;Lee, Sang-Rak
    • Journal of Animal Science and Technology
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    • v.49 no.5
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    • pp.633-638
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    • 2007
  • This study was conducted to determine the protein requirement level in growing Jindo dog through nitrogen balance experiment. Twelve female dogs aged 18~20 weeks old were allotted one of 3 dietary treatments containing 21, 23 and 25% of crude protein. Average daily gain of dogs fed experimental diets containing 21, 23 and 25% of crude protein were 65.42, 79.58 and 99.17g/d, respectively, and there was a significant difference between 21 and 25% of crude protein treatments(p<0.05). Retained nitrogen were calculated 0.74, 0.96 and 1.31g/kgBW.75/d for dogs fed diets containing 21, 23 and 25% of crude protein, respectively, and were significantly higher(p<0.05) in dogs fed 25% of crude protein diet then those of other dogs. A quadratic regression equation was calculated between nitrogen intake(x) and nitrogen retention(y); y=-2.519x2+12.79x-14.79, and it was found a significantly(p<0.05) higher regression coefficient of 0.782. From the above equation, it was estimated maintenance requirement of crude protein for growing Jindo dog is 11.25g/kg BW.75/d.

Magnetic Resonance Imaging Diagnosis of Epidural Idiopathic Sterile Pyogranulomatous Inflammation in a Dog

  • Hwang, Taesung;Shin, Changho;Kim, Youngki;Yeon, Seongchan;Lee, Hee-chun
    • Journal of Veterinary Clinics
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    • v.34 no.5
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    • pp.377-380
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    • 2017
  • An 8-year-old, shih-tzu female dog was referred due to neurological signs including paraparesis and back pain. On the complete blood count, hematologic analysis showed elevated leukocytosis. Serum biochemical analysis revealed elevated serum alkaline phosphatase concentration and C-reactive protein concentration. On the neurologic exam, the dog was suspected to have thoracolumbar myelopathy. On magnetic resonance imaging, there were masses within the spinal canal at L1-3 intervertebral disc space that were located dorsal to spinal cord. It was hyperintense on T1-, T2-weighted magnetic resonance images, Fluid-attenuated inversion recovery, and fat suppression images. The contrast-enhanced T1-weighted images showed no enhancement. The lesions were well circumscribed. The spinal cord was compressed and displaced ventrally by the mass. After removal of the masses via L1-L3 dorsal laminectomy, pyogranulomatous inflammation was confirmed by histopathological examination. Six months after surgery, the dog recovered uneventfully and remained fully ambulatory with no neurological deficits. This case demonstrates the utility of magnetic resonance imaging for the diagnosis of spinal canal pyogranulomatous inflammation.

Binding of Nalidixic Acid with Plasma Protein -On the Species Difference in Binding- (Nalidixic Acid와 혈장단백(血漿蛋白)과 결합(結合)에 관(關)한 연구(硏究) -동물(動物)의 종속차(種屬差)에 대(對)하여-)

  • Kim, Shin-Keun
    • Journal of Pharmaceutical Investigation
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    • v.6 no.1
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    • pp.14-17
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    • 1976
  • Binding of nalidixic acid with plasma of male and female rats, dogs, and rabbits was studied in vitro using the method of equilibrium dialysis in 1/15 mole phosphate buffer (pH 7.4). Rat plasma had the most extensive binding capacity followed by dog and rabbit plasma, and the plasma of female had more extensive capability than male in rat and rabbit but it was reversed in dog.

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C-Kit-Negative Gastrointestinal Stromal Tumor in the Stomach

  • Seo, Ho Seok;Hyeon, Ji Yeon;Shin, Ok-Ran;Lee, Han Hong
    • Journal of Gastric Cancer
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    • v.15 no.4
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    • pp.290-294
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    • 2015
  • C-kit-negative gastrointestinal stromal tumors (GISTs) are uncommon, and there have been few reports about the diagnosis and treatment of c-kit-negative GISTs in the stomach. We report the case of a patient who was diagnosed with a huge and atypical GIST in the stomach. The GIST was completely resected and finally diagnosed as c-kit-negative GIST based on immunohistochemical staining of tumor cells, which were negative for CD117 and CD34 and positive for Discovered on GIST-1 (DOG1). C-kit-negative GISTs could be treated by complete resection and/or imatinib, which is the same treatment for c-kit-positive GISTs.

Nutritional evaluation of new alternative types of dog foods including raw and cooked homemade-style diets

  • Boyeon Choi;San Kim;Goo Jang
    • Journal of Veterinary Science
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    • v.24 no.5
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    • pp.63.1-63.9
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    • 2023
  • Background: New alternative types of pet foods such as raw and cooked homemadestyle diets containing human food ingredients have been introduced due to a trend of pet humanization and diversification of consumer needs. Objectives: To evaluate nutritional adequacy of new alternative types of dog foods containing human food ingredients as maintenance diets for dogs. Methods: Eleven homemade-style foods for adult dogs were purchased from online channel in Korea and analyzed to evaluate nutritional adequacy for adult dogs. Nutrients analyzed included crude protein, amino acids, crude fat, fatty acids, and minerals. Results: Crude protein and amino acids in all products satisfied Association of American Feed Control Officials (AAFCO) requirements. Crude fat in one of 11 products did not meet AAFCO requirements. The most deficient minerals were selenium (10 of 11, 90.9%), copper (five of 11, 45.5%), zinc (five of 11, 45.5%), potassium (three of 11, 27.3%), calcium (three of 11, 27.3%), iron (two of 11, 18.2%), and magnesium (one of 11, 9.1%). Six products were not in the range of the recommended Ca:P ratio in AAFCO dog food maintenance nutrient profiles. Conclusions: This study performed nutritional evaluation of raw and cooked homemadestyle foods as maintenance diets for adult dogs. Some nutritional inadequacies were observed including some minerals, Ca:P ratio, and omega-6:omega-3 fatty acid ratio, although three products (26.2%) satisfied the AAFCO standard except selenium. Overall, the data suggest a need for accurate nutritional adequacy statement for consumers based on proper methods to validate the formula.

A Case of Canine Babesia spp. Infection (Caine Babesia spp. 감염증예)

  • Chae Joon-Seak;Ihn Dong-Chul;Han Jae-Chul;Kim Nam-Soo;Lee Joo-Muk;Choi In-Hyuk
    • Journal of Veterinary Clinics
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    • v.6 no.1
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    • pp.185-191
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    • 1989
  • A dog which was hospitalized to Veterinary Teaching Hospital, College of Veterinary Medicine, Jeonbug National University on December 28, 1988 was revealed severe anemia: hemoglobinuria and weakness. In the inspections, abdominal pain and spleno megaly at the ventral abdomen were detected by palpations. In the examinations of blood, the obtained results were summarized as follows: Babesla spp. was identified on the blood smear stained with Giemsa. The Babesia spp. was assumed to the Babesia gibsoni for the their small size and pleomorphism such as comma form, ring form and dot form. In the blood examinations of the patient, Ht: 22.5%, RBC:354${\times}$10$^4$/${\mu}\ell$, Hb: 8.8g/dl, serum protein: 8g/dl, and WBC count was 21, 425/${\mu}\ell$. In the chemical examinations of serum, the value of AST(GOT) was 30iu and ALT(GPT) was 20iu, respectively. The blood sugar was 60mg/d1. In the urine test, urine protein was 30mg/d1 and the hemoglobin In the urine was the +++ and occult blood reaction(Benzidine test) in the feces was +++. Splenomegaly was confirmed by X-ray examination. To confirm for the Babesia spp. infection, 5ml of the whole blood of the patient(3% of Parasitized erythrocytes) were inoculated into the cephalic vein of the two normal dogs. In the blood of experimental dogs which were inoculated parasitized blood, Babesia spp. was detected in the two doss and pleomorphic parasites were observed, too. In the blood examinations of No. 1 the Ht and RBC were decreased to 6.8% and 52${\times}$10$^4$/${\mu}\ell$, respectively. WBC count was 10.600/${\mu}\ell$ and serum protein was 6.8g/dl. The rates of parasitized erythrocytes were 15% in the experimental dog. Also +++ of the hemoglobin was detected in the urine. In the X-ray examination, splenomegaly was comfirmed and it was confirmed by autopsy of the experimental dog(No. 1).

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A case of protein losing nephropathy in a dog infected with canine Babesia gibsoni

  • Kim, Ha-Jung;Park, Chul;Jung, Dong-In;Kang, Byeong-Teck;Kim, Ju-Won;Lim, Chae-Young;Park, Hee-Myung
    • Korean Journal of Veterinary Research
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    • v.46 no.1
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    • pp.77-81
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    • 2006
  • A 1-year and 6-month-old, intact male, Shih-tzu dog was referred to the Konkuk University Veterinary Teaching Hospital, due to ascite, melena, severe anemia, and polyuria/polydipsia. Complete blood count showed moderate leukocytosis, lymphocytosis, monocytosis, and microcytic hypochromic nonregenerative anemia. On serum biochemistry profiles, hypoalbuminemia and hypoproteinemia were observed, and proteinuria was detected on urinalysis. Pleural effusion and mild ascites were noted in radiological findings, and bilateral nephromegaly was noted on ultrasonography. Babesia gibsoni was detected by PCR. Babesiosis and glomerular disease was treated at a time with prednisolone and buparvaquone. This therapeutic regime was very efficacious and clinical sings were rapidly improved. Proteinuria and severe anemia were disappeared following therapy.