• Title/Summary/Keyword: DNP

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Inhibitory Effects of Scrophulariae Radix on β-hexosaminidase release and cytokine production in RBL-2H3 cells (현삼(玄蔘) 추출물이 RBL-2H3 비만세포에서 β-hexosaminidase 및 cytokine 분비에 미치는 효과)

  • Kim, Se-Gie
    • The Korea Journal of Herbology
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    • v.32 no.6
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    • pp.9-15
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    • 2017
  • Objectives : Traditional medicines isolated from natural products often have positive effects in the prevention and healing of various immune disorders, such as allergy and atopic inflammation. Scrophulariae Radix (SR) been used in oriental medicine used for treatment of acute and chronic inflammatory diseases. Mast cells are known to play important roles in the initiation of allergic reactions. In this study, we investigated the effects of SR ethanol extract on inflammatory responses in IgE-stimulated RBL-2H3 mast cells. Methods : Rat basophilic leukemia RBL-2H3 cells were purchased from Korean Cell Line Bank (KCLB No. 22256). Cell viability was measured by MTT assay. Assays for ${\beta}-Hexosaminidase$ Secretion : RBL-2H3 cells were sensitized with dinitrophenyl-ImmunoglobulinE (DNP IgE). The next antigen DNP-BSA ($25ng/m{\ell}$) was added for 10 minutes and the reaction was terminated after 5 minutes in the ice bath. To determine ${\beta}-Hexosaminidase$ release, supernatants were aliquoted into 96-well plates. Samples were mixed with substrate solution and incubated for 1 h at $37^{\circ}C$. Absorbance was measured with a spectrophotometer at 405 nm. IL-4 and tumor necrosis $factor-{\alpha}$($TNF-{\alpha}$) concentrations in cell culture supernatants were measured using enzyme-linked immunosorbent assay (ELISA) kits. Results : The cytotoxicity of SRE in RBL-2H3 cells was less than 5%. SRE inhibited DNP-IgE-imduced degranulation of mast cells in RBL-2H3 cells. Also significantly decreased the levels of inflammatory cytokine, IL-4 and TNF-alpha. In this study, the SRE showed potential anti-allergic and antiinflammatory. Conclusions : These results indicate that SRE could be inhibit the allergic response through suppressing the mast cell activation.

A Study on State Estimation Based Intrusion Detection in Power Control Systems Using DNP3 over TCP/IP (DNP3 over TCP/IP 환경 전력 제어시스템에서의 상태추정 기반 침입 탐지 연구)

  • Hyeonho Choi;Junghee Lee
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.34 no.4
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    • pp.615-627
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    • 2024
  • With the evolution of power systems and advancements in IT technology, there is an increasing demand to shift from serial-based communication to TCP/IP-based communication. However, TCP/IP communication entails various security threats, necessitating extensive consideration from an information security perspective. Security measures such as authentication and encryption cannot be rapidly implemented due to issues like the replacement of Remote Terminal Units (RTUs) and the performance requirements of encryption algorithms. This paper proposes a state estimation-based intrusion detection model to identify and effectively detect threats to power control systems in such a context. The proposed model, in addition to signature detection methods, verifies the validity of acquired data, enabling it to detect attacks that are difficult to identify using traditional methods, such as data tampering.

A Study of Accumulated Ecosystem Carbon in Mt. Deogyusan, Korea (덕유산의 생태계 탄소축적량 산정에 관한 연구)

  • Jeong, Seok-hee;Eom, Ji-young;Jang, Ji-hye;Lee, Jae-ho;Cho, Koo-hyun;Lee, Jae-seok
    • Korean Journal of Environmental Biology
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    • v.33 no.4
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    • pp.459-467
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    • 2015
  • Understanding of a carbon storage in a regional scale ecosystem is a very important data for predicting change of global carbon cycle. Therefore, the real data collected in the various ecosystems are a very useful for enhancing accuracy of model prediction. We tried to estimate total accumulated ecosystem carbon in Deogyusan National Park (DNP) with naturally well preserved ecosystem. In DNP, vegetations were classified to four main communities with Quercus mongolica community (12,636.9 ha, 54.8%), Quercus variabilis community (2,987.0 ha, 13.0%), Pinus densiflora community (5,758.0 ha, 25.0%), and Quercus serrata community (402.9 ha,1.7%). Biomass and soil carbons were estimated by the biomass allometric equations based on the DBH and carbon contents of litter and soil (0~30 cm) layers collected in 3 plots ($30cm{\times}30cm$) in each community. The biomass and soil carbons were shown as high value as 1,759,000 tC and 7,776,000 tC, respectively, in Quercus mongolia community in DNP area. In Quercus mongolica, Quercus variabilis, Quercus serrata, Pinus densiflora communities, the accumulated ecosystem carbon were shown 9,536,000 tC, 1,405,000 tC, 147,000 tC, 346,000 tC, respectively. Also, the total ecosystem carbon was estimated with 11,434,000 tC in DNP.

Effects of Perilla frutescens Extract on Anti-allergic Reactions in a Mouse Model (소엽 추출물이 마우스모델에서 항알레르기 반응에 미치는 영향)

  • Ko, Jung-A;Lim, Hun-Sun;Kim, Gun-Hee;Park, Ji-Yong;Han, Chan-Kyu;Park, Hyun-Jin
    • Korean Journal of Food Science and Technology
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    • v.42 no.4
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    • pp.488-493
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    • 2010
  • We investigated the effect of Perilla frutescens (PF) ethanol extract powder (PF-E30) on the local allergic reaction activated by anti-DNP IgE and the mast cell-mediated immediate-type allergic reactions induced by compound 48/80 in a mouse model. One gram of PF powder extracted with 30% ethanol at $80^{\circ}C$ contained 12.3 mg of rosmarinic acid. Oral administration of PF-E30 (0.1 to 0.5 mg/kg body weight) significantly reduced plasma histamine levels and inhibited histamine release from peritoneal mast cells in mice activated by compound 48/80 or anti-DNP IgE. Moreover PF-E30 dose-dependently inhibited the production of antigen-induced IgE. These results indicate that the PF ethanol extract inhibits mast cell-mediated immediate-type allergic reactions in vivo and in vitro.

Inhibitory Effect of Saururus chinensis (Lour.) Baill Extracts on Allergy in Mouse Models (Mouse 모델 알레르기 반응에서 삼백초(三白草)가 미치는 영향)

  • Suk, Min-Hee;Kang, Kyung-Hwa;Choi, Yung-Hyun;Choi, Byung-Tae;Lee, Yong-Tae
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.19 no.1
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    • pp.146-151
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    • 2005
  • We investigated the effect of Saururus chinensis (Lour.) Baill (SCB) on allergy in mice. We conformed compound 48/80-induced mesenteric mast cell degranulation, active systemic anaphylatic shock and histamine release. Also observed acetic acid-induced vascular permeability and anti-dinitrophenyl (DNP) IgE-mediated passive cutaneous anaphylaxis. SCB inhibited mesenteric mast cell degranulation and active systemic anaphylatic shock induced by compound 48/80 dose-dependently. When SCB was pretreated by intra-peritoneal injection, the plasma histamine levels were reduced. SCB also significantly inhibited acetic acid-induced vascular permeability and anti-DNP IgE-mediated passive cutaneous anaphylaxis. In addition, SCB reduced IL-10 mRNA expression of the lung on ovalbumin-induced allergy. These results indicate that SCB inhibits allergy.

The Antiallergic and Antioxidative Effects of Chaenomeles Sinensis (CS) in RBL 2H3 Cells (목과(木瓜) 추출물이 항알레르기 및 항산화 작용에 미치는 영향)

  • Lee, Seung-Yeon
    • The Journal of Pediatrics of Korean Medicine
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    • v.24 no.2
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    • pp.126-136
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    • 2010
  • Objectives The purpose of this study was to investigate the antiallergic and antioxidative effects of Chaenomeles Sinensis (CS). Methods CS pretreatments inhibited anti-DNP IgE in RBL-2H3 mast cells for an hour. we measured cell viability, $\beta$-hexosaminidase release, IL-4, TNF-$\alpha$ secretion, and IL-4, TNF-$\alpha$ mRNA expression CS pretreatments inhibited DNP-HSA($10\;{\mu}g/ml$) for ten minutes, we measured Dicholrodihydrofluorescein(DCF) and DPPH radical-scavenging activity in 0.5 mM 1,1-diphenyl-2-picrydrazyl(DPPH) radical solution, 0.1ml, 99% ethanol 0.8ml, and CS 0.1 ml mixed solution. Results 0, 1, 2, 3, 4, 5 mg/ml CS treatments were not affect on cell viability and inhibited b-hexosaminidase release, IL-4, TNF-$\alpha$ secretion, CS treatments also decreased IL-4, TNF-$\alpha$ mRNA expression in RBL-2H3 cells. CS treatments inhibited reactive oxygen species(ROS) and DPPH radical-scavenging activity. Conclusions These results suggest that CS may be useful for the prevention or treatment of allergic disease.

Monitoring system design&Implementation using wireless solution (무선 통신을 사용한 모니터링 시스템의 설계 및 구현)

  • Jeon Yoon-Ho;Woo Jong-Hyun;Kim Young-Ryun;Kim Hee-Dong
    • 한국정보통신설비학회:학술대회논문집
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    • 2002.08a
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    • pp.39-42
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    • 2002
  • RS-232C는 정보기기들을 연결하는 직렬통신 표준으로, 간단한 구조로 인해, 복잡한 요구 조건이 없는 상용 기기들의 제어 및 감시용으로 사용되고 있다. 최근 무선동신기술이 발전함에 따라, 저가격의 무선통신 기술을 활용하여 무선 RS-232C에 대한 요구가 늘어나고 있다. 무선 RS-232C로 기존 유선의 RS-232C를 대체함으로서, 기존 시스템의 변경없이 편리한 접속기능을 대체한 수 있을 것이다. 본 논문에서는 유선 RS-232C를 대체하는 무선 RS-232C 모듈을 채용하여, 전력제어에 사용하는 배전자동화 장치의 관리를 간편하게 하는 시스템의 구현에 대해서 다루고 있다. 배전 자동화시스템은 일종의 SCADA시스템의 일부로서, 통신 프로토콜로서 산업용 DNP3.0을 사용하고 있다. 무선 RS-232C 링크의 신뢰성을 확보하기 위하여, 무선 구간에 DNP3.0을 채택하였으며, 무선 통신방식으로는 무선 RF, 블루투스(Bluetooth), 무선랜(wireless LAN)등 3가지 방식을 채용하여, 설계, 구현내용을 기술하였다.

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Removal of heavy metal and Hydrogen sulfide/Nitrophenol using Mackban-stone (맥반석을 이용한 중금속과 악취물질/nitrophenol의 제거)

  • Quen, Zhe-Xue;Yin, Cheng-Ri;Jin, Yin-Shu;Seok, Mi-Soo;Lee, Sung-Taik
    • Journal of the Korea Organic Resources Recycling Association
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    • v.9 no.2
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    • pp.45-51
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    • 2001
  • Mackban-stone effectively removed heavy metals, such as Fe, Cu, Cd, and Zn, with best removal of Fe and Cu. And the removal of heavy merals related with ion exchange of Ca. Mackban-stone is also an efficient deodorant of hydrogen sulfide and ammonia and inhibited the growth of E coli. The degradation rare of 4-nitropheno1 by Nocardioides sp. PNP101 and 2,4-dinitrophenol by Strain CJ1 and Rhodococcus sp. DNP 505 are increased by Mackbane-srone.

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Effect of Kamihyungbangjihwang-tang on Immediate-type Allergic Reaction (가미형방지황탕이 즉시형 알러지반응에 미치는 영향)

  • Kim Kwang Sik;Lee Dong Hee;Ko Dae Woong;Song Jung Mo;Eun Jae Soon
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.17 no.5
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    • pp.1270-1275
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    • 2003
  • The purpose of this research was to investigate the effects of Kamihyungbangjihwang-tang (KHT) on immediate-type allergic reaction. KHT was composed of hyungbangjihwangtang (HJT) and Rubus coreanus Miquel (RC) and Aspalathus linear is (AL). KHT and HJT (500 mg/kg) inhibited the systemic anaphylaxis induced by compound 48/80 and inhibited the passive cutaneous anaphylaxis (PCA) induced by anti-dinitrophenyl (DNP)-IgE and DNP-human serum albumin (HSA) in vivo. In addition, KHT, HJT, RC and AL inhibited the release of histamine and increased the release of cAMP from rat peritoneal mast cells. The anti-allergic action of KHT was more potent than those of HJT. These results indicate that KHT may be useful for the prevention and treatment of type I allergy related disease via inhibition of histamine release from mast cells.

Effect of Bopaewon-tang on Allergic Reaction (보폐원탕(補肺元湯)이 알러지반응에 미치는 영향)

  • Jeon Yong-Keun;Leem Jae-Yoon;Song Jung-Mo;Eun Jae-Soon
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.19 no.6
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    • pp.1604-1609
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    • 2005
  • The purpose of this research was to investigate the effects of Bopaewon-tang (BT) on allergic reaction. In the present study, we examined the effect of BT on type 1 and type tV allergic reaction. BT (500 mg/kg) did not affect the systemic anaphylaxis induced by compound 48180 and the passive cutaneous anaphylaxis induced by anti-dinitrophenyl (DNP)-IgE and DNP-human serum albumin in vivo. Also, BT did not affect the release of histamine from peritoneal mast cells in rats. In addition, BT did not affect the permeability of evans blue into peritoneal cavity, but inhibited the writhing syndrome induced by acetic acid. BT inhibited the delayed type hypersensitivity induced by SRBC and the contact dermatitis induced by dinitrofluorobenzene. These results indicate that BT may be useful for the prevention and treatment of type IV allergy related disease.