• 한국환경성돌연변이발암원학회지
• /
• 제17권2호
• /
• pp.71-77
• /
• 1997

The single cell gel electrophoressis(SCGE) assay, also known as the comet assay, is a rapid, simple, visual and sensitive technique for measuring and analysing DNA breakage in mammalian cells. The SCGE or comet assay is a promising test for the detection of DNA damage and repair in individnal cells. It has widespread potential applications in DNA damage and repair studies, genotoxicity testing and biomonitoring. In this microgel electrophoresis technique, cells are embedded in agarose gel on microscope slides, iysed and electrophoresed under alkaline conditions. Cells with increased DNA damage display increased migration of DNA from the nucleus towards the anode. The length of DNA migration indicates the amount of DNA breakage in the cell. The comet assay is also capable of identifying apoptotic cells which contain highly fragmented DNA. Here we review the development of the SCGE assay, existing protocols for the detection and analysis of comets, the relevant underlying principles determining the behaviour of DNA and the potential applications of the technique.

• Communications for Statistical Applications and Methods
• /
• 제13권3호
• /
• pp.525-535
• /
• 2006

We describe tests for detecting and locating quantitative traits loci (QTL) for traits in Hanwoo. Lod scores and a permutation test have been described. From results of a permutation test to detect QTL, we select major DNA markers of ILSTS098 microsatellite locus in Hanwoo chromosome 2 for further analysis. K-means clustering analysis applied to four traits and eight DNA markers in ILSTS098 resulted in three cluster groups. We conclude that the major DNA markers of BMS1167 microsatellite locus in Hanwoo chromosome 2 are markers 105bp, 113bp and 115bp. Finally, bootstrap testing method has been adapted to calculate confidence intervals and for finding major DNA Markers.

• 한국식품과학회지
• /
• 제19권3호
• /
• pp.212-219
• /
• 1987

재래종 적색 자두 (Prunus salicina)에서 효소를 추출하여 4종류의 polyphenol화합물과 반응시켜 얻어진 갈변반응 생성물에 대하여 Bacillus subtilis H17과 M45를 이용한 rec-assay와 Salmonella typhimurium TA98과 TA100 두 균주를 이용한 Ames test, 그리고 calfcthymus DNA를 이용하는 DNA절단시험을 이용하여 돌연변이원성과 돌연변이 억제작용을 조사하였다. 포자 rec-assay 에서는 pyrogallol, hydroxyhydroquinone, 3,4-dihydroxytoluene, chlorogenic acid 의 갈변반응 생성물은 모두 DNA손상능력이 없었으며 8가지 금속이온 중 ${Zn}^{2+}$과 ${Ni}^{2+}$의 첨가로 고초균 DNA손상에 약한 영향을 나타내었다. DNA절단시험 결과 4종류 갈변반응 생성물 모두 DNA절단작용이 없었으며 금속이온의 영향에 있어서는 pyrogallol 갈변반응 생성물이 ${Cu}^{2+}$의 영향을 받아 ${Cu}^{2+}$의 농도가 증가함에 따라 강한 절단작용을 나타내었으며 3,4-dihydroxytoluene 과 hydroxyhydroquinone갈변반응 생성물은 금속이온의 영향을 전혀 받지 않았다. 또한 chlorogenic acid갈변반응 생성물은 DNA 절단을 억제하는 효과를 나타내었다. Ames test에서는 4가지 갈변반응 생성물 모두 변이원성은 없었으며 benzo$[{\alpha}]$pyrene을 사용한 변이원성 억제작용 실험결과 benzo$[{\alpha}]$pyrene의 활성을 강하게 억제하는 것으로 나타났다.

• 식물분류학회지
• /
• 제48권4호
• /
• pp.289-300
• /
• 2018

Coreanomecon is a monotypic and endemic genus in Korea, distributed mainly in the southern regions. Coreanomecon is morphologically similar to Hylomecon by producing red latex, easily distinguished from Chelidonium, which produces yellow latex. Coreanomecon were merged into Hylomecon or Chelidonium depending on the authors. To understand the phylogenetic relationship of Coreanomecon, DNA sequences of chloroplast rbcL and matK and nuclear Internal Transcribed Spacer (ITS) regions were determined from the species of Papaveroideae (Papaveraceae) in Korea and analyzed with the Maximum Parsimony and Bayesian methods. Phylogenetic analyses of Papaveroideae suggest that Coreanomecon is sister to the clade of Chelidonium and Stylophorum in the ITS data and that it is sister to Hylomecon in the chloroplast (cpDNA) data. A constraining analysis using the Shimodaira-Hasegawa test (S-H test) suggested that the ITS data do not reject the sister relationship of Coreanomecon and Hylomecon. The S-H test also suggested that the cpDNA data is compatible with the placement of Coreanomecon as a sister to the clade of Chelidonium and Stylophorum. Although the conflicting phylogenetic results may stem from insufficient phylogenetic signals, they may also be associated with hybridization between Hylomecon and an ancestor of Stylophorum and Chelidonium. The results of this study suggest that Coreanomecon is a distinct lineage as an endemic genus, supporting the morphological data.

• 대한의생명과학회지
• /
• 제28권3호
• /
• pp.157-169
• /
• 2022

DNA typing is the typical technology in the forensic science and plays a significant role in the personal identification of victims and suspects. Short tandem repeat (STR) is the short tandemly repeated DNA sequence consisting of 2~7 bp DNA units in specific loci. It is disseminated across the human genome and represents polymorphism among individuals. Because polymorphism is a key feature of the application of DNA typing STR analysis, STR analysis becomes the standard technology in forensics. Therefore, the DNA database (DNA-DB) was first introduced with 4 essential STR markers for the application of forensic science; however, the number of STR markers was expanded from 4 to 13 and 13 to 20 later to counteract the continuously increased DNA profile and other needed situations. After applying expanded STR markers to the South Korean DNA-DB system, it positively affected to low copy number analysis that had a high possibility of partial DNA profiles, and especially contributed to the theft cases due to the high portion of touch DNA evidence in the theft case. Furthermore, STR marker expansion not only contributed to the resolution of cold cases but also increased kinship index indicating the potential for improved kinship test accuracy using extended STR markers. Collectively, the expansion of the STR locus was considered to be necessary to keep pace with the continuously increasing DNA profile, and to improve the data integrity of the DNA-DB.

• 한국동물학회지
• /
• 제20권4호
• /
• pp.159-168
• /
• 1977

계대 배양중인 생쥐의 임파종양 L5178Y 세포의 유전자돌연변이 유발성 검출법(Methotrexate-저항성)과 순수분리한 사람의 임파구에서의 DNA 회복복제법을 사용하여 Salmomella/microsome 시스템에서 돌연변이 유발성이 확인된 살충데 DDVP와 trichlorfon, 살균제 TMTD 및 제초제 MO와 NIP등 5종의 농약이 포유동물 세포에 미치는 유전적 영향을 조사했다. 조사한 농약중 TMTD는 상기조사한 시스템 모두에서 양성결과를 보여준 반면에 DDVP와 trichlofon은 L5178Y 세포에서의 돌연변이 유발성은 나타내지 않았으나 DNA회복 복제법에서는 양성결과를 보여주었다. MO와 NIP는 조사한 시스템 모두에서 양성결과를 나타내지않았다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권19호
• /
• pp.8063-8067
• /
• 2014

Background: Cervical cancer is a major public health problem in Bangladesh. Persistence of high risk human papillomavirus (HRHPV) influences the progression of the disease, with an important role in followup for cervical intraepithelial neoplasia (CIN). Objective: To establish application of high risk HPV DNA test in the follow-up of women after treatment of CIN. Materials and Methods: This cross-sectional and hospital based study was carried out among 145 CIN treated women during the previous six months to three years at the colposcopy clinic of Bangabandhu Sheikh Mujib Medical University, Dhaka, between January 2011 and June 2012. Pap smear and HPV samples were collected and colposcopy was performed to find out the persistence of the disease. Cervical samples obtained were tested for HPV DNA using the Hybrid Capture II (HC-II) test. A cervical biopsy was collected whenever necessary. The results were compared to assess the efficacy of different methods during follow up such as Pap smear, HPV test and colposcopy. Results: Mean age of the recruited women (n=145) was 33.6 (${\pm}7.6$), mean age of marriage was 16.8 (${\pm}2.9$) and mean age of 1st delivery was 18.8 (${\pm}3.5$) years. More than half had high grade CIN before treatment and 115 (79.3%) women were managed by LEEP and 20.7% were managed by cold coagulation. Among the 145 treated women, 139 were negative for HPV DNA and six of them (4.1%) were HPV positive. Sensitivity of Pap smear (40.0) and HPV DNA test (40.0) was poor, but specificity was quite satisfactory (>93.0) for all the tests. Conclusions: The high risk HPV DNA test can be an effective method of identifying residual disease. It can be added to colposcopy and this should be applied to all treated women attending for their first or second post-treatment follow-up visit at 6 months to one year, irrespective of the grade of treated CIN.

• 한국식품위생안전성학회지
• /
• 제31권1호
• /
• pp.1-7
• /
• 2016

본 연구의 목적은 NIH3T3와 HepG2 세포에서 에탄올 유도 세포독성 및 유전독성에 대하여 녹차엑기스(GTE)와 epigallocatechin-3-gallate (EGCG)의 보호작용을 평가하는데 있다. 세포생존율은 MTT assay를 실시하였으며 DNA 손상도는 Comet assay로 실시한 결과 에탄올은 농도의존적인 세포독성과 유전독성을 나타내었다. 한편 GTE와 EGCG는 에탄올 유도 세포독성 및 DNA 손상에 대하여 유의성 있는 억제효과를 나타내었으며 DPPH시험과 LDL oxidation 및 8OH-2'dG 생성시험에서 항산화효과를 나타내었다. 한편 녹차성분 함유 시판 리큐르주도 순수 에탄올에 비하여 세포독성억제 및 DNA 손상억제효과를 나타내었다. 이상의 시험결과 GTE와 함유 EGCG는 항산화성 유전독성억제기전을 통한 에탄올독성저감 물질로 판단된다.

• 분석과학
• /
• 제21권1호
• /
• pp.48-52
• /
• 2008

본 연구에서는 사건 현장 증거물에서 타액반의 확인을 위해 개발된 $SALIgAE^{(R)}$ 시약의 유효성을 검토하였다. $SALIgAE^{(R)}$ 검사법의 상세한 작용 기작에 대해서는 상업적 이유 등으로 잘 알려져 있지 않아 실험을 통해 민감도와 특이성을 검토하였으며, 이를 기존의 타액검사 방법인 아가로스 겔 확산법 및 $Phadebas^{(R)}$ 검사법과 비교하였다. 사건 현장에서 경제적이며 쉽고 신속하게 타액검사를 수행할 수 있도록 $SALIgAE^{(R)}$검사법을 변형하였는데, 5분 이내에 1/600 이상 희석된 타액까지 확인이 가능하였다. 타액 이외의 인체 분비물(정액, 질액, 뇨, 땀, 콧물)은 5분 이내에 $SALIgAE^{(R)}$ 검사 양성반응을 보이지 않았다. 또한 $SALIgAE^{(R)}$ 검사 시약은 상온에서도 높은 안정성을 보여 법과학 실험실에서는 물론 사건 현장에서도 유용하게 사용할 수 있을 것으로 판단되었다.

• 대한한의학회지
• /
• 제44권4호
• /
• pp.72-86
• /
• 2023

Objectives: The medical field is rapidly evolving with AI and digital technologies like AI-based X-ray analysis and digital therapeutics gaining approval. Telemedicine is becoming prominent, and medical schools are adapting by integrating AI education. Pusan National University leads a talent training project for AI in health. Korean Medicine is incorporating AI with diagnostic systems and chatbots. However, there's a lack of research on education awareness in Korean Medicine Colleges. The study aims to assess opinions on integrating AI, digital therapeutics, and DNA test into the Korean medicine college curriculum for improved education. Methods: We selected appropriate four specific areas: artificial intelligence in medicine, digital therapeutics, DNA test, and telemedicine. The questionnaire developed for this study underwent expert evaluation and was subsequently administered to registered KMDs of the Association of Korean Medicine, as well as students from 12 Korean Medicine universities. The survey was designed to analyze the awareness and perceived importance of the 4 areas. Results: Both KMDs and Korean medicine students exhibited comparable awareness levels across the four objectives. Notably, both groups identified a high educational necessity and importance of artificial intelligence in medicine for clinical settings. Statistically significant differences were observed between KMDs and students in their perspectives on the importance of telemedicine and DNA test in the Korean medicine field, the educational necessity of DNA test within Korean medicine universities, and the need for comprehension of regulations related to digital therapeutics. Conclusion: The survey of Korean medicine professionals and students underscores a strong understanding of key areas such as Telemedicine, medical AI, DNA test, and digital therapeutics. Medical AI is identified as crucial for future education. There's a consensus on the need for curriculum changes in Korean medicine schools, particularly in adapting to evolving healthcare trends. The focus should be on practical clinical application, with a call for additional research to better integrate student and practitioner perspectives in future curriculum reform discussions.