• BMB Reports
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• v.30 no.3
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• pp.200-204
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• 1997

We eliminated introns from replication independent chicken H3.3 histone gene using a H3.3 cDNA clone and a genomic H3.3 clone. After introduction into Rat 3 cells, we observed its pattern of expression by analyzing mRNA from different phases of the cell cycle. Even without introns, the H3.3 gene was expressed constitutively at a low level throughout the cell cycle. This indicates that the introns in the H3.3 gene are not responsible for the cell cycle-independent expression of the gene. This result contradicts previous reports that suggested their importance in cell cycle regulated expression. We believe that other regions of the gene, promoter, coding region, and/or 3'-end of the gene, are involved in its expression pattern.

• Biomedical Science Letters
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• v.8 no.3
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• pp.185-188
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• 2002

We have cloned the gene fur long QT syndrome in Korean genome and determined its detailed nucleotide sequence. Blood DNAs were isolated from 68 healthy individuals (including males and females) and the genomic DNAs were amplified by PCR method followed by automatic DNA sequencing. Entire sequence of the coding region for KCNEI was located in exon 3. PCR products were reexamined for the confirmation of KCNE1-specific amplification by nested PCR. KCNE1 mRNA was 436 bp. This corresponded to 129 amino acids. There was no recognizable difference between males and females. This study should contribute to the better understanding of long QT syndrome in Korean population.

• BMB Reports
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• v.30 no.1
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• pp.80-84
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• 1997

The synthesis and secretion of human angiogenin in E. coli by the natural leader sequence has been studied. We constructed a recombinant plasmid containing human angiogenin cDNA which encompassed all the coding region including leader sequence required for secretion. The recombinant plasmid was introduced into a suitable E. coli host. The angiogenin was detected in the culture medium and periplasm upon the induction of gene expression. The molecular weight of the secreted angiogenin was identical to that of authentic angiogenin purfied from human plasma when estimated by SDS-PAGE and immunoblotting. showing that the natural leader sequence was recognized and processed by the secretion machinery of E. coli. The angiogenin concentration in the culture medium reached a maximum within 2 h when expressed at $37^{\circ}C$ with 0.02~2 mM IPTG. In contrast, the expression level increased gradually over time up to 11 h at $23^{\circ}C$ with 0.002~2 mM IPTG and at $37^{\circ}C$ with 0.002 mM IPTG.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.3
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• pp.253-255
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• 1999

Reverse transcription and polymerase chain reaction (RT-PCR) assay was used to detect SMV strains. A pair of oligonucleotide primers were designed to include the cylindrical inclusion (CI) coding region between 4,176 to 5,560 nt. Amplification from the total RNA extracted from infected plants with SMV yielded a 1,385 bp DNA fragment. RT-PCR was shown to be $10^3$ times more sensitive than the ELISA assay and it could detect a virus in $10^{-6}$ dilution. Restriction enzyme analysis of RT- PCR products using EcoR I showed that SMV isolates were classified into six groups according to the patterns of restriction fragments.

• Journal of Microbiology and Biotechnology
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• v.19 no.9
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• pp.966-971
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• 2009

Peroxidase from Coprinus cinereus (CiP) has attracted attention for its high specific activity and broad substrate spectrum compared with other peroxidases. In this study, the functional expression of this peroxidase was successfully achieved in the methylotrophic yeast Pichia pastoris. The expression level of CiP was increased by varying the microbial hosts and the expression promoters. Since a signal sequence, such as the alpha mating factor of Saccharomyces cerevisiae, was placed preceding the cDNA of the CiP coding gene, expressed recombinant CiP (rCiP) was secreted into the culture broth. The Mut Pichia pastoris host showed a 3-fold higher peroxidase activity, as well as 2-fold higher growth rate, compared with the $Mut^s $ Pichia pastoris host. Furthermore, the AOX1 promoter facilitated a 5-fold higher expression of rCiP than did the GAP promoter.

• Proceedings of the KIEE Conference
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• 2006.07d
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• pp.2142-2143
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• 2006

Today any data storage system cannot satisfy all of these conditions, however holographic data storage system can perform faster data transfer rate because it is a page oriented memory system using volume hologram in writing and retrieving data. System can be constructed without mechanically actuating part therefore fast data transfer rate and high storage capacity about 1Tb/cm3 can be realized. In this research, to reduce errors of binary data stored in holographic data storage system, a new method for bit error reduction is suggested. Firstly, find fuzzy rule to use test bed system for Element of Holographic Digital Data System. Secondly, make fuzzy rule table using DNA coding method. Finally, reduce prior error element and recording digital data. Recording ratio and reconstruction ratio show good performance.

• Journal of the Korean Data and Information Science Society
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• v.18 no.2
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• pp.489-506
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• 2007

Gene structure prediction, which is to predict protein coding regions in a given nucleotide sequence, is the most important process in annotating genes and greatly affects gene analysis and genome annotation. As eukaryotic genes have more complicated structures in DNA sequences than those of prokaryotic genes, analysis programs for eukaryotic gene structure prediction have more diverse and more complicated computational models. There are Ab Initio method, Similarity-based method, and Ensemble method for gene prediction method for eukaryotic genes. Each Method use various algorithms. This paper introduce how to predict genes using HMM(Hidden Markov Model) algorithm and present the process of gene prediction with well-known gene prediction programs.

• 제어로봇시스템학회:학술대회논문집
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• 2002.10a
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• pp.70.1-70
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• 2002

In the fuzzy applications and theories, it is very important to consider how to design the optimal fuzzy model from short training data, in order to construct the reasonable fuzzy model for identifying the practical process. There are several concerns to be confirmed for efficient fuzzy model design. One of concern is the optimization problem of the fuzzy model. In various applications, the genetic algorithm is widely applied to obtain optimal fuzzy model and other cases that adopt evolutionary mechanism of the nature. If we use natural selection and multiplication operation of the genetic algorithm, early convergence to local minimum can be occurred. In other word, we can find only optimum...

• Parasites, Hosts and Diseases
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• v.40 no.1
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• pp.59-64
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• 2002

A Cryptosporidium parvum sporozoite and oocyst λgt11 cDNA library was screened with a hyperimmune rabbit serum that was developed against insoluble fragments of ultrasonicated oocysts. A clone named Cp22.4.1 encoding a protein of 231 amino acids with 4 zinc-finger domains characterized by a Cys-X2-Cys-X4-His-X4-Cys motif was isolated and characterized. There was a complete match between the sequencing data of the coding region of Cp22.4.1 and the corresponding gene at chromosomal level. Cloning in a pBAD-TOPO-TA expression vector permitted to evaluate the antigenicty of the recombinant His-tagged antigen. This antigen was recognized by 2 out of 5 sera from Cruptosporidium immune calves and not by sera from parasite naive animals.

• Proceedings of the Korea Contents Association Conference
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• 2013.05a
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• pp.75-76
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• 2013

유전자 염기서열의 직접적인 변화 대신 후성기작을 통해 유전자 발현이 조절되는 후성유전은 크게 DNA 메틸화(methylation), 히스톤 변형(modification), ncRNA(non-coding RNA)로 제어가 가능하다. 후성유전을 이해하기 위해 노화 관련 유전자를 대상으로 데이터베이스를 구축하고 전반적인 연구결과를 살펴보고자 한다. 유전자의 프로모터(promoter), CpG island(CGI) 부위에 메틸화가 될 경우 다른 부위에 비해 유전자 발현에 큰 영향을 주므로, 특히 CGI 부위를 중심으로 전체 유전자 그룹과 노화 관련 유전자 그룹간의 분포도를 비교 분석하였다. 또한 ncRNA 중 miRNA와 노화 유전자와의 상호작용을 분석하였다. 이와 같은 분석접근 방법은 노화 관련 유전자의 조절을 이해하는데 도움이 될 것으로 사료된다.