Biomedical Science Letters (대한의생명과학회지)
- Volume 8 Issue 3
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- Pages.185-188
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- 2002
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- 1738-3226(pISSN)
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- 2288-7415(eISSN)
Complete Nucleotide Sequence of KCNE1 in Korean Genome
- Yeo, Shin-Il (Department of Biology, Keimyung University) ;
- Kim, Su-Won (Department of Biology, Keimyung University) ;
-
Kim, Yoon-Nyun
(Department of Cardiology, Keimyung University Dongsan Medical Center)
;
- You, Kwan-Hee (Department of Biology, Chungnam University) ;
- Shin, Song-Woo (Wooridul Spine Hospital) ;
- Kim, Myoung-Hee (Department of Anatomy, Yonsei University College of Medicine) ;
-
Song, Jae-Chan
(Department of Veterinary Science, Kyungpook National University)
;
-
Yoo, Min
(Department of Biology, Keimyung University)
- Published : 2002.09.01
Abstract
We have cloned the gene fur long QT syndrome in Korean genome and determined its detailed nucleotide sequence. Blood DNAs were isolated from 68 healthy individuals (including males and females) and the genomic DNAs were amplified by PCR method followed by automatic DNA sequencing. Entire sequence of the coding region for KCNEI was located in exon 3. PCR products were reexamined for the confirmation of KCNE1-specific amplification by nested PCR. KCNE1 mRNA was 436 bp. This corresponded to 129 amino acids. There was no recognizable difference between males and females. This study should contribute to the better understanding of long QT syndrome in Korean population.