• BMB Reports
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• v.43 no.9
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• pp.609-613
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• 2010

The Dvl and Axin proteins, which are involved in the Wnt signaling pathway, each contain a conserved DIX domain in their sequences. The DIX domain mediates interaction between Dvl and Axin, which together play an important role in signal transduction. However, the extremely low production of DIX domain fragments in E. coli has prevented more widespread functional and structural studies. In this study, we demonstrate that the DIX domains of Dvl and Axin are expressed noticeably in a multi-cistronic system but not in a mono-cistronic system. Formation of the $DIX_{Dvl1}-DIX_{Axin1}$ complex was investigated by affinity chromatography, SEC and crystallization studies. Unstable DIX domains were stabilized by complexing with counterpart DIX domains. The results of the preliminary crystallization and diffraction of the $DIX_{Dvl1}-DIX_{Axin1}$ complex may prove useful for further crystallographic studies.

• BMB Reports
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• v.38 no.2
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• pp.243-247
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• 2005

Dishevelled (Dvl) is a positive regulator of the canonical Wnt signaling pathway, which regulates the levels of $\beta$-catenin. The $\beta$-catenin oncoprotein depends upon the association of Dvl and Axin proteins through their DIX domains, and its accumulation directs the expression of specific developmental-related genes at the nucleus. Here, the $^1H$, $^{13}C$, and $^{15}N$ resonances of the human Dishevelled 2 DIX domain are assigned using heteronuclear nuclear magnetic resonance (NMR) spectroscopy. In addition, helical and extended elements are identified based on the NMR data. The results establish a structural context for characterizing the actin and phospholipid interactions and binding sites of this novel domain, and provide insights into its role in protein localization to stress fibers and cytoplasmic vesicles during Wnt signaling.

• Geophysics and Geophysical Exploration
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• v.22 no.4
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• pp.225-238
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• 2019

Migration velocity analysis (MVA) for creating optimum depth-domain velocities in seismic imaging was applied to marine long-offset multi-channel data, and the effectiveness of the MVA approach was demonstrated by the combinations of conventional data processing procedures. The time-domain images generated by conventional time-processing scheme has been considered to be sufficient so far for the seismic stratigraphic interpretation. However, when the purpose of the seismic imaging moves to the hydrocarbon exploration, especially in the geologic modeling of the oil and gas play or lead area, drilling prognosis, in-place hydrocarbon volume estimation, the seismic images should be converted into depth domain or depth processing should be applied in the processing phase. CMP-based velocity analysis, which is mainly based on several approximations in the data domain, inherently contains errors and thus has high uncertainties. On the other hand, the MVA provides efficient and somewhat real-scale (in depth) images even if there are no logging data available. In this study, marine long-offset multi-channel seismic data were optimally processed in time domain to establish the most qualified dataset for the usage of the iterative MVA. Then, the depth-domain velocity profile was updated several times and the final velocity-in-depth was used for generating depth images (CRP gather and stack) and compared with the images obtained from the velocity-in-time. From the results, we were able to confirm the depth-domain results are more reasonable than the time-domain results. The spurious local minima, which can be occurred during the implementation of full waveform inversion, can be reduced when the result of MVA is used as an initial velocity model.

• 한국신재생에너지학회:학술대회논문집
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• 2005.06a
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• pp.638-642
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• 2005

For gas hydrate exploration, long offset multichannel seismic data acquired using by the 4km streamer length in Ulleung basin of the East Sea. The dataset was processed to define the BSRs (Bottom Simulating Reflectors) and to estimate the amount of gas hydrates. Confirmation of the presence of Bottom Simulating reflectors (BSR) and investigation of its physical properties from seismic section are important for gas hydrate detection. Specially, faster interval velocity overlying slower interval velocity indicates the likely presences of gas hydrate above BSR and free gas underneath BSR. In consequence, estimation of correct interval velocities and analysis of their spatial variations are critical processes for gas hydrate detection using seismic reflection data. Using Dix's equation, Root Mean Square (RMS) velocities can be converted into interval velocities. However, it is not a proper way to investigate interval velocities above and below BSR considering the fact that RMS velocities have poor resolution and correctness and the assumption that interval velocities increase along the depth. Therefore, we incorporated Migration Velocity Analysis (MVA) software produced by Landmark CO. to estimate correct interval velocities in detail. MVA is a process to yield velocities of sediments between layers using Common Mid Point (CMP) gathered seismic data. The CMP gathered data for MVA should be produced after basic processing steps to enhance the signal to noise ratio of the first reflections. Prestack depth migrated section is produced using interval velocities and interval velocities are key parameters governing qualities of prestack depth migration section. Correctness of interval velocities can be examined by the presence of Residual Move Out (RMO) on CMP gathered data. If there is no RMO, peaks of primary reflection events are flat in horizontal direction for all offsets of Common Reflection Point (CRP) gathers and it proves that prestack depth migration is done with correct velocity field. Used method in this study, Tomographic inversion needs two initial input data. One is the dataset obtained from the results of preprocessing by removing multiples and noise and stacked partially. The other is the depth domain velocity model build by smoothing and editing the interval velocity converted from RMS velocity. After the three times iteration of tomography inversion, Optimum interval velocity field can be fixed. The conclusion of this study as follow, the final Interval velocity around the BSR decreased to 1400 m/s from 2500 m/s abruptly. BSR is showed about 200m depth under the seabottom