• Title/Summary/Keyword: DIG

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Memory, deconstruction and reconstruction of 'history': Suzan-Lori Parks' The America Play ('역사'의 기억과 해체 그리고 재구성: 수잔-로리 팍스의 "미국 극")

  • Park, Jin-Sook
    • English Language & Literature Teaching
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    • v.15 no.3
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    • pp.315-332
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    • 2009
  • The purpose of this paper is to scrutinize how Parks recalling, deconstructing and reconstructing African-American memories of the absences in American history through a black Lincoln impersonator named, The Foundling Father or The Lesser Known. Parks unearths and reconstitutes a significance for the historical event of Lincoln's assassination by repetitive mimicry and verbal puns. As a pun of the Founding Father, the Foundling Father reminds us of Abraham Lincoln, one of the most venerated figures in American history. In the first act, the black Foundling Father performs as The Great Man. This inverted minstrel show of the black Foundling Father performing a white Lincoln exposes the desire of the Foundling Father to insert his narrative within the history of America. With a series of assassinations, the African-American performers figuratively murder the power and control of the American myth. In the second act, his wife Lucy and his son Brazil dig relics from the past out of 'The great hole of history' instead of the Foundling Father. Digging and burial for African-Americans are their livelihood and their calling as well. As Parks pointed out, they should locate the ancestral buried ground, dig for bones and find bones because so much of African-American history has been unrecorded, dismembered and washed out. Parks leaves the possibilities of digging and burying on the black history through Lucy and Brazil.

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A Study on the Implementation of a 40kbps Narrowband Powerline Communication Modem Using the Chirp Technique (Chirp 방식을 이용한 40kbps급 협대역 전력선 통신 모뎀 구현에 관한 연구)

  • Park, Sung-Wook;Ohm, Woo-Yong
    • Journal of the Institute of Electronics Engineers of Korea TE
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    • v.42 no.4
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    • pp.53-62
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    • 2005
  • In this paper, we implement a 40kbps narrowband powerline communication modem which is able to high transfer rate than conventional powerline communication technology by using the chirp technique that is one of the spread spectrum techniques. A modem that implement in this paper used the CEBus standard and a symbol that the linear variable frequency signal of 100$\sim$380kHz is kept for 25us. Experimental results, we verified the possibility that communication technology with the Chirp technique can make transfer rate more than 40kbps at narrowband communication.

Digoxigenin-labeled in situ Hybridization for Diagnosis of Aujeszky's disease in Pigs (Digoxigenin-labeled in situ Hybridization을 이용한 오제스키병의 진단)

  • Kim, Soon-Bok;Moon, Oun-Kyung;Song, Geun-Suk;Yeo, Sang-Geon
    • Korean Journal of Veterinary Pathology
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    • v.1 no.2
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    • pp.119-124
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    • 1997
  • The objective of this study was to develop digoxigenin (DIG)-labeled in situ hybridization (ISH) test for diagnosis of Aujeszky's Disease(AD) in infected organs. Specific DNA with well conserved gene sequences encoding gp50 antigen in AD virus (ADV) was obtained by Polymerase Chain Reaction (PCR) method. A pair of oligonucleotide primers used in PCR allowed amplification of a 217 bp sequence from the gp50 ADV gene. The DNA was then labeled with DIG by primer labeling method for use as probe in ISH test to detect ADV nucleic acids in various tissue. Positive hybridization was demonstrated by dark pigmentation in nuclei and cytoplasm of ADV infected cells particularly in brain tonsillar crypt epithelium and pulmonary alveolar cells. This result suggests that ISH is a valuable sensitive and rapid diagnostic test for AD.

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A Study on application of Trapezoidal Steel Box Tunnelling Method (지중압입체를 이용한 지하구조물 축조방법의 적용성 연구)

  • Jun, Sung Bai
    • Journal of the Society of Disaster Information
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    • v.4 no.2
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    • pp.138-154
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    • 2008
  • The conventional non-dig underground structure building method which made an appearance to reduce the social and environmental costs and maximize the efficiency of the social overhead capital facilities could not help being uneconomical because of many problems such as unnecessary excessive excavation, water leakage, obstacle interference, difficulty of curvilinear application and connection complexity between propelled and injected bodies due to indiscriminate application of small and large circular steel pipes without consideration of the site conditions. The T.S.T.M, in which a protruded square tube is applied as a propulsion and injection body in a design that considered site conditions such as ground condition, depth of soil and live load, was able to be economical as it solved the problems of water resistance, minimization of obstacle interference and curvilinearity, and we can see that it can be applied to all grounds by utilizing or complementing the target ground in terms of engineering. Also in configuring the transverse section, it is possible to not only secure excellent structural safety but also implement all of the above engineering characteristics not only in the square cross section but also in the arch cross section, so it was possible to build structures on any section or ground, and we could confirm the LCC reduction effect and the VE effect.

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A Data Driven Index for Convergence Sensor Networks (융합 센서 네트워크를 위한 데이터 기반 색인)

  • Park, Jeong-Seok
    • Journal of the Korea Convergence Society
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    • v.7 no.6
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    • pp.43-48
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    • 2016
  • Wireless sensor networks (WSN) can be more reliable and easier to program and use with the help of sensor database management systems (SDMS). SDMS establish a user-friendly SQL-based interface to process declarative user-defined queries over sensor readings from WSN. Typical queries in SDMS are ad-hoc snapshot queries and long-running, continuous queries. In SDMSs queries are flooded to all nodes in the sensor net, and query results are sent back from nodes that have qualified results to a base station. For query flooding to all nodes, and result flooding to the base station, a lot of communication energy consuming is required. This paper suggests an efficient in-network index solution, named Distributed Information Gathering (DIG) to process range queries in a sensor net environment that can save energy by reducing query and result flooding.

Characterization of growth hormone-like sequence of loach, Misgurnus mizolepis (미꾸라지 성장 호르몬 염기 서열의 특성에 대하여)

  • Kim, Jin-Kyung;Song, Young-Hwan
    • Journal of fish pathology
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    • v.7 no.2
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    • pp.95-103
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    • 1994
  • We have prepared cDNA libray of loach. M. mizolepis in order to isolate cDNA clone of growth hormone gene. Total RNA was isolated from pituitary of loach, and then mRNA was further purified from total RNA by oligo (dT)-coupled magnetic beads. The purified mRNA was used as substrates to prepare cDNA. The resulting cDNA was ligated into the EcoRV/Smal site of pBlueKS+. The ligation mixture have transformed E. coli JM109 strain with electroporator to obtain high yield of transformation efficiency. All the transformants was screened with DIG-labeled Tilapia growth hormone gene by high density colony hybridization. After isolating 10 putative colonies showing the positive signals, secondary colony hybridization and southern hybridization could confirm it as true clones. The nucleotide sequence of one candidate, pCGHI, was compared with 312 bp DNA fragment used as DNA probe and show 52% relative homology to Tilapia growth hormone gene.

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Development of DNA probe for a protistan parasite of tunicate Halocynthia roretzi

  • Choi, Dong-Lim;Hwang, Jee-Youn;Choi, Hee-Jung;Hur, Young-Baek
    • Journal of fish pathology
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    • v.23 no.3
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    • pp.313-322
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    • 2010
  • Edible tunicate Halocynthia roretzi, one of the most commercially important aquatic organisms in Korea, has been killed by tunic softness syndrome since last decade. The intracellular protistan parasite observed by the transmission electron microscope in hemocytes of the tunicate was considered to be the causative agent of the mass mortality. The goal of the present work is to examine the characteristic features of the parasite by identifying the 18S rDNA sequences of the parasite. The experiments conducted include amplification of presumptive 18S rDNA from diseased tunicate tissues with UNonMet-PCR and sequencing the product. A preliminary phylogenetic analysis was performed on the presumptive parasite rDNA. A digoxigenin labeled DNA probe was designed on the basis of the sequences of rDNA. Dig-ISH assay was conducted to diagnose the protistan parasite. A PCR using UNonMet-PCR primer generated 595 bp SSU rDNA fragment. Subsequently, PCRs with primer pair expended this sequence to 1542 bp. This is the first partial sequences of SSU rDNA gene to be published on the protistan parasite that has presumed causing the mass mortality of tunicate. Since the Dig-ISH technique demonstrated the presence of infection in hemocytes on the all host tissues, the fragment was confirmed to be the intracellular protistan parasite SSU rDNA. A phylogenetic analysis suggested that the protistan parasite may be a unique eukaryote that is closely related to Apicomplexa.

Advanced Tools for Modeling, Design and Optimization of Wind Turbine Systems

  • Iov Florin;Hansen Anca Daniela;Jauch Clemens;Sorensen Poul;Blaabjerg Frede
    • Journal of Power Electronics
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    • v.5 no.2
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    • pp.83-98
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    • 2005
  • As wind turbine technology and control has advanced over the last decade, this has led to a high penetration of wind turbines into the power system. Whether it be for a large wind turbine or an offshore wind farm with hundreds of MW power capacity, the electrical system has become more and more important in controlling the interaction between the mechanical system of the wind turbine and the main power system. The presence of power electronics in wind turbines improves their controllability with respect not only to its mechanical loads but also to its power quality. This paper presents an overview of a developed simulation platform for the modeling, design and optimization of wind turbines. The ability to simulate the dynamic behavior of wind turbines and the wind turbine grid interaction using four simulation tools (Matlab, Saber, DIgSILENT and HAWC) is investigated, improved and extended.

Revisiting Social Discount Rates for Public Investment

  • SONG, JOONHYUK
    • KDI Journal of Economic Policy
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    • v.39 no.2
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    • pp.75-98
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    • 2017
  • This paper aims to estimate the social discount rate (SDR) rather than dig into its theoretical foundation. As SDRs can be derived by investigating both the rate of return on investment and the social time preference rate, we estimate the marginal productivity of both private and public capital and the time preference rate based on the Euler equation. In order to provide a single representative SDR, the weighted averages of the marginal productivity and time preference rate, whose weights are determined by the flow of funds data reflecting the social demand of funds, are presented. Based on the empirical results, we argue that the marginal productivity of private capital stands in the middle of the 3% range while that of public capital varies from 4.5% to 8.6%, with the time preference rate showing a decreasing trend from 3.2% in the early 2000s to 1.2% by around 2030. The single representative SDR or the weighted SDR is estimated to be approximately 3.0~4.5% and expected to continue its downward trend for the foreseeable future.