• 벤처다이제스트
• /
• 제2호통권127호
• /
• pp.60-63
• /
• 2009

• 벤처다이제스트
• /
• 제1호통권126호
• /
• pp.58-61
• /
• 2009

• 한국지반공학회:학술대회논문집
• /
• 한국지반공학회 2005년도 춘계 학술발표회 논문집
• /
• pp.1267-1274
• /
• 2005

The system which can manage and analyse the tunnel information in design and construction stage was developed through several years' research named ITIS(Intelligent Tunnel Information System). Now, the system is being modified and improved for the use of international tunnel construction information sharing. Some countries have their own management system for tunnel construction, and one of them, called DEST(Data Evaluation System for Tunneling), is adopted for the international cooperation for this research of development. The aimed system is operated under world wide web environment.

• 생물정신의학
• /
• 제6권1호
• /
• pp.67-73
• /
• 1999

The cAMP-dependent protein kinase(PKA) is an intracellular enzyme with serine-threonine kinase activity that plays a key role in cell growth, differentiation, and apoptosis in eukaryotes. In order to understand the PKA signal transduction pathway regulating cell life cycle and identify its role, we focused on the characterization of up-/down-regulated genes by PKA using the differential display polymerase chain reaction. Seven differentially expressed sequence tags(DEST) have been obtained. Among these DESTs, 2 DESTs were homologous to the sequence of genes from BLAST search result. KC1-5 DEST that was up-regulated in A123.7 cells was highly corresponded to mouse apoptosis-related gene(MA-3) or mouse mRNA for topoisomerase inhibitor suppressed(TIS). MA-3 was induced in various types of apoptosis, specially in NGF-deprived apoptotic PC12 cells. TIS was down-regulated in the RVC lymphoma cells incubated with topoisomerase inhibitor that induces DNA strand breakages. PG1-1 DEST that was highly expressed in PC12 cells was corresponded to transposon Tn10 3'-end. Tnansposon Tn10 was up-regulated in differentiated myeloblastic ML-1 cells by 12-O-tetradecanoylphorbol-13-acetate. This study illuminates that MA-3/TIS was down-regulated by PKA activity, and transposon Tn10 was up-regulated by it.

• 한국방재학회:학술대회논문집
• /
• 한국방재학회 2007년도 정기총회 및 학술발표대회
• /
• pp.612-615
• /
• 2007

The environmental damage caused by river-improvement and slope of incision cliff can dest habitations of animate nature and it takes long time for restoration. River system constructed with stability of flood control, fine biotope, harmony with surroundings should be need to protect and restore nature. Therefore, in this study, conduct comparative experiment in various methods which are stable hydraulically and develop vegetation on revetment of bank, stream bed protection and disaster prevention aspect.

• 식물병연구
• /
• 제11권1호
• /
• pp.48-55
• /
• 2005

Cylindrocarpon destructans는 인삼 및 수목에 뿌리썩음병을 일으키는 토양 전염병 식물병원균이다. 신속 정확한 검출 가능성을 알아보기 위하여 종 특이적인 primer와 nested PCR 기법을 활용하여 인삼 유묘로부터 뿌리썩음 병균 C. destructans로 2차 PCR증폭을 실시한 결과 병원성이 확인된 C.destructans에서만 400bp의 종특이적 증폭산물을 얻을 수 있었다. 종 특이성 primer 와 nested PCR 기법을 이용한 인삼뿌리썩음병균 DNA에 대한 반응 민감도는 최저 약 1fg으로 나타나 단 몇 개의 포자만 존재해도 검출이 가능하였다. 또한, nested PCR 기법은 실제 이병토양에 심었을 경우에도 C.destructans 에 감염된 인삼 유묘로부터만 정확하게 병원균을 검출해 내었다. 종특이적 primer 와 nested PCR 기법을 이용한 본 연구 결과는 실제 재배농가에서 인삼 경작시 뿌리썩음병 진단에 매우 유용하게 활용될 수 있을 것으로 판단된다.

• Journal of international Conference on Electrical Machines and Systems
• /
• 제1권1호
• /
• pp.84-90
• /
• 2012

Various methods have been proposed to identify the flux position in an interior permanent magnet synchronous motor (IPMSM) without the use of mechanical sensors. To achieve this, a method that uses both the back electromotive force (EMF) and the saliency to identify the flux position in the IPMSM without the injection of high-frequency components at low speeds has been reported. This method was then extended in order to drive the motor with no load to a light load. We propose a combination of these methods with different proportional-integral (PI) controllers for controlling $di_{dest}$/dt and $di_{qest}$/dt. We also introduce compensation values $F_L$ and $F_H$ to reduce the position error when the estimation rule is being selected.

• Mycobiology
• /
• 제38권1호
• /
• pp.33-38
• /
• 2010

We have successfully applied the nested PCR to detect Cylindrocarpon destructans, a major pathogen causing root rot disease from ginseng seedlings in our former study. The PCR assay, in this study, was used to detect the pathogen from soils. The nested PCR using internal transcribed spacer (ITS) 1, 4 primer set and Dest 1, 4 primer set maintained the specificity in soils containing various microorganisms. For a soil DNA extraction method targeting chlamydospores, when several cell wall disrupting methods were tested, the combination of lyophilization and grinding with glass beads, which broke almost all the chlamydospores, was the strongest. The DNA extraction method which was completed based on the above was simple and time-saving because of exclusion of unnecessary stages, and efficient to apply in soils. As three ginseng fields whose histories were known were analyzed, the PCR assay resulted as our expectation derived from the field information. The direct PCR method will be utilized as a reliable and rapid tool for detecting and monitoring C. destructans in ginseng fields.

• Asian Pacific Journal of Cancer Prevention
• /
• 제13권7호
• /
• pp.3015-3023
• /
• 2012

The mitochondrial antioxidant protein manganese superoxide dismutase (MnSOD) may represent a new type of tumor suppressor protein. Overexpression of the cDNA of this gene by plasmid or recombinant lentiviral transfection in various types of cancer leads to growth suppression both in vitro and in vivo. We previously determined that changes in MnSOD expression had bidirectional effects on adriamycin (ADR) when combined with nitric oxide (NO). Radiation induces free radicals in a manner similar to ADR, so we speculated that MnSOD combined with NO would also have a bidirectional effect on cellular radiosensitivity. To examine this hypothesis, TE-1 human esophageal squamous carcinoma cells were stably transfected using lipofectamine with a pLenti6-DEST plasmid containing human MnSOD cDNA at moderate to high overexpression levels or with no MnSOD insert. Blastidicin-resistant colonies were isolated, grown, and maintained in culture. We found that moderate overexpression of MnSOD decreased growth rates, plating efficiency, and increased apoptosis. However, high overexpression increased growth rates, plating efficiency, and decreased apoptosis. When combined with NO, moderate overexpression of MnSOD increased the radiosensitivity of esophageal cancer cells, whereas high MnSOD overexpression had the opposite effect. This finding suggests a potential new method to kill certain radioresistant tumors and to provide radioresistance to normal cells.

• 한국항해학회지
• /
• 제20권2호
• /
• pp.1-13
• /
• 1996

Most of the ShipHandling Simulators of full-mission-bridge system need vast area to install and even PC-based maneuvering simulators are often equipped with Steering Wheel or Engine Telegraphe etc. of data input interface, which necessarily makes the user face with excessive financial burden. These have been one of the obstacles for the officers, captains, pilots and students in access to maneuvering simulation whenever they want to try it in advance prior to actual ship maneuvering. Subsequently, all the officers and captains come to have little chances to train themselves until they arualified as a pilot after a long period of time of realship maneuvering practice on board, which means they have to control they have to control their own ship at sea without clear understanding on her maneuverability when they are forced to do it on the way. And these lack of capability for maneuvering have used so often to result in marine casualties of collision with other ships or pier facilities while maneuvering in harbor. To prevent those accidents by means of enhancing their maneuvering ability, PC-based DeskTop Simulator that allows anyong to access readily at anytime is needed and in conformation to such demand this simulator has been developed. The Software this simulator written in Turbo Pascal Ver. 5.0 has adopted MMG mathmatical model theoretically in part and also it was designed to make it possible that all numeric data inputs and outputs with graphic presentation for maneuvering operation be carried out just only with keyboard and monitor console. With the Simulation software, all the officers, captains, pilots and even students who has a proper computer at hand are expected to be able to make an attempt to simulate the maneuvering of their ownship or any other types of them at any port in which they want to do it.