• Journal of Biosystems Engineering
• /
• v.41 no.4
• /
• pp.365-372
• /
• 2016

Purpose: Tea is the most frequently consumed drink worldwide, next to water. About 75% of the total world tea production includes black tea, and withering is one of the major processing steps critical for the quality of black tea. There are two types of tea withering methods: physical and chemical withering. Withering can be achieved by using tat, tunnel, drum, and trough withering systems. Of these, the trough withering system is the most commonly used. This study focuses on the different types of withering, their effect on the various quality attributes of tea, and other aspects of withering methods that affect superior quality tea. Results: During physical withering, tea shoots loose moisture content that drops from approximately 70-80% to 60-70% (wet basis). This leads to increased sap concentration in tea leaf cells, and turgid leaves become flaccid. It also prevents tea shoots from damage during maceration or rolling. During chemical withering, complex chemical compounds break down into simpler ones volatile flavor compounds, amino acids, and simple sugars are formed. Withering increases enzymatic activities as well as the concentration of caffeine. Research indicates that about 15% of chlorophyll degradation occurs during withering. It is also reported that during withering lipids break down into simpler compounds and catechin levels decrease. Improper withering can cause adverse effects on subsequent manufacturing operations, such as maceration, rolling, fermentation, drying, and tea storage. Conclusion: Freshly harvested leaves are conditioned physically and chemically for subsequent processing. There is no specified withering duration, but 14-18 h is generally considered the optimum period. Proper and even withering of tea shoots greatly depends on the standards of plucking, handling, transportation, environmental conditions, time, and temperature. Thus, to ensure consumption of high quality tea, the withering step must be monitored carefully.

• Biomolecules & Therapeutics
• /
• v.29 no.4
• /
• pp.399-409
• /
• 2021

1,2-Diacetylbenzene (DAB) is a metabolite of 1,2-diethylbenzene, which is commonly used in the manufacture of plastics and gasoline. We examined the neurotoxic effects of DAB in young and old rats, particularly its effects on hippocampus. Previously, we reported DAB impairs hippocampal neurogenesis but that the underlying mechanism remained unclear. In this study, we evaluate the toxicities exhibited by DAB in the hippocampi of 6-month-old (young) and 20-month-old (old) male SD rats by treating animals intraperitoneally with DAB at 3 mg/kg/day for 1 week. Hippocampal areas were dissected from brains and RNA was extracted and subjected to RNA-seq analysis. RNA results showed animals exhibited age-dependent sensitivity to the neurotoxic effects of DAB. We observed that inflammatory pathways were up-regulated in old rats but that metabolism- and detoxification-related pathways were up-regulated in young rats. This result in old rats, especially upregulation of the TREM1 signaling pathway (an inflammatory response involved in Alzheimer's disease (AD)) was confirmed by RT-PCR. Our study results provide a better understanding of age-dependent responses to DAB and new insight into the association between DAB and AD.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2022.05a
• /
• pp.92-92
• /
• 2022

산 사면의 지반이 붕괴되어 흙, 모래, 자갈 그리고 물 등이 혼합하여 유동하는 토석류는 예측과 대비가 어려운 자연재해 중 하나 이다. 특히, 강우로 인해 발생하는 토석류의 경우 매우 빠르게 유동하기 때문에 피해 예측이 제한적이다. 이러한 토석류가 도심지역 또는 마을주변에서 발생할 경우 많은 인명 및 재산 피해가 발생한다. 따라서 토석류의 유동을 최소화시키기 위해선 1차적으로 수치모형을 통한 전반적인 유동 및 피해 규모 예측이 이루어져야 하며, 이러한 분석을 바탕으로 사방댐과 같은 구조물의 효율적인 설계가 이루어져야 한다. 이에 수치모형을 통해 토석류의 유동을 분석하고자 하는 많은 연구가 진행된 바 있으며, 사방댐 설계 분석 또한 수치모형과 실험을 통해 연구된 바 있다. 선행연구들에 따르면, 1) 발생부로부터의 거리, 2) 토석류 에너지의 감소, 3) 침식-연행 작용, 4) 사방댐의 용량 등이 효율적인 사방댐 설계에 영향을 미친다고 분석된 바 있다. 하지만 위의 항목들에 대한 종합적인 비교분석은 미비한 실정이다. 따라서 본 연구에선 위에서 제시한 4가지의 항목들을 바탕으로 사방댐 설계에 중요한 요소를 평가하고 산정하고자 한다. 토석류의 유동과 사방댐을 모의분석하기 위해 Deb2D 수치모형을 활용하였으며, Voellmy 유변학적 모형과 침식-연행-퇴적 작용을 분석할 수 있는 알고리즘을 사용하여 토석류의 유동을 현실에 가깝게 모의하였다. 2011년 서울 우면산에서 발생한 산사태 유역들 중에서 래미안 아파트 유역과2019년 강원도 갈남리에서 발생한 산사태를 대상지구로 선정하였다. 연구 결과에 따르면 4가지 요소들 중에서 사방댐의 용량이 효율적인 사방댐 설계에 가장 주요한 요인으로 분석되었다.

• Korean Journal of Optics and Photonics
• /
• v.14 no.4
• /
• pp.413-422
• /
• 2003

We have calculated the power extraction efficiency and the lasing wavelength distribution of index-coupled DFB lasers at threshold for various kL and facet reflectivity combinations, and compared with those above-threshold. The power extraction efficiency increases as the asymmetry of the facet reflectivities increases. The power extraction efficiency above-threshold is slightly larger than that at threshold. Since the relative photon density around the center region increases as kL increases, the power extraction efficiency decreases. The uniformity of the distribution of lasing wavelength over the stop band increases due to the relief of mode degeneracy as the asymmetry of the facet reflectivities increases. In the case of AR-HR combination, the lasing wavelength distributions at threshold are similar to those above-threshold. However, in the case of AR-AR combination, the lasing wavelength at threshold is concentrated on both edges of the stop band, while it is concentrated only on the longer wavelength edge above-threshold. As kL increases, the range of the lasing wavelength distribution increases due to the increase of the stop band. The effect of AR reflectivity on the power extraction and the lasing wavelength distribution is very weak.

• Journal of Embryo Transfer
• /
• v.25 no.1
• /
• pp.9-14
• /
• 2010

This study was conducted to find out the effects of artificial shrinkage (AS) on post-thaw development of bovine embryos. The blastocoelic cavity of blastocyst was punctured to remove its fluid contents and then incubated in the holding medium (HM) for 10 min. The punctured and non-punctured (control) blastocysts were equilibrated in vitrification solution 1 (VS1; TCM-199+20% FBS+10% EG) for 5 min and vitrification solution 2 (VS2; TCM199+20% FBS+35% EG+5% PVP+0.5 M Sucrose) for 1 min and vitrified by direct dropping into the liquid nitrogen. Vitrified blastocysts (punctured and control) were thawed and cultured in vitro (12 hr) for studying survival and hatching rates. The levels of shrinkage were measured by the volume of the blastocyst during equilibration in VS1 (at 1, 3 and 5 min of equilibration) and VS2 (at 30 and 60 sec of equilibration) that was considering the volume of non-punctured blastocyst in HM as 100%. The levels of shrinkage were higher in punctured group (62.4, 64.6, 64.3% at 1, 3 and 5 min in VS1; 50.6 and 52.7% at 30 and 60 sec in VS2) than control group (84.8, 86.6, 86.4% at 1, 3 and 5 min in VS1; 72.1 and 68.8% at 30 and 60 sec in VS2), but within each group the levels of shrinkage were similar. The survival (90.9%) and hatching (50.0%) rates of vitrified blastocysts at 12 hr post-thaw were higher in punctured group than that in control group (76.9% and 0.0% respectively). We confirmed that vitrification solutions (VS1 and VS2) have no toxic effect on the survival of blastocysts because the survival rates of blastocysts exposed to VS1 and VS2 for 24 hr were similar between punctured and control groups (94.3 vs. 96.0%; p>0.05). In conclusion, the preliminary data show that AS of blastocyst may improve survival and hatching rate after thawing.

• Journal of Embryo Transfer
• /
• v.26 no.4
• /
• pp.277-282
• /
• 2011

The present research was carried out to evaluate the possibility of increasing female offspring production ratios using artificial insemination buffer (AIB) before artificial insemination (AI). In this experiment, we optimized AIB composition, made an AIB gun and analyze factors affecting AI non-return rate after AIB treatment. The AIB was made with the base of Tris-buffer supplemented with L-arginine and several other chemicals that might reduce the motility of male sperm compared to the female counterpart, therefore, increasing the possibility of fertilization by female sperm. AIB must be deposited into $2^{nd}$ to $4^{th}$ cervix by AIB gun. After 15 min of AIB deposition, frozen semen was deposited into the same place. A total of 348 cattle were inseminated with AIB insemination, and there were no significant differences between AIB and traditional AI non-return rates (56.8% vs. 55.7%). The AI non-return rate in AIB group, however, differed significantly among 7 Hanwoo farms. The parturition numbers ($1^{st}$ to $7^{th}$) of cows did not affect AIB AI rate. The proportion of AIB AI success rates was significantly higher in Hanwoo cows than in dairy cows (61.0% vs. 48.7%), but the average AI success rate did not differ significantly between AIB and conventional AI (56.8% vs. 55.7%). The female offspring production rate in $2^{nd}$ to $4^{th}$ cervix deposition place was significantly higher than that in the uterus body (77.7% vs. 59.6%, p<0.05). The injection volume of AIB in 5 and 10 ml was significantly higher than that in 2 ml (77.7%, 78.7% vs. 51.8%, p<0.05), but there were no differences in AIB injection volume between 5 and 10 ml. The best exposure time of AIB in the cervix was 10 to 15 min rather than 5 min (79.2%, 77.2% vs. 52.6%, p<0.05). AIB therefore needs to have an exposure time of at least over 10 min for a higher production rate of female offspring. In conclusion, AIB could be used in AI industry to increase the female offspring ratio and AIB AI can increase the AI success rate.

• The Journal of Art Theory & Practice
• /
• no.1
• /
• pp.145-168
• /
• 2003

Im 18. Jahrhundert stritt man urn allgemeing$\"{u}$ltige Kriterien f$\"{u}$r die Kunstkritik, welche ebenda aufzubl$\"{u}$hen begann. Im Zentrum dieser Auseinandersetzung, die uns als "Laokoon-streit" bekannt ist, befanden sich Johann Joachim Winckelmann einerseits und ein franz$\"{o}$sischer Bildhauer Etienne-Maurice Falconet(1716-1791) andererseits. Die beiden waren von ganz unterschiedlichen Kunstideen $\"{u}$berzeugt. Obwohl sie niemals zusammentraf, fielen sie in eine heftige schriftliche Diskussion. Wie bekannt, vertrat Winckelmann den aufkommenden Klassizismus. Er setzte die griechische Kunst auf die h$\"{o}$chste Rangstufe auf und bezeichnet sie als Tr$\"{a}$ger der sogenannten "edlen Einfalt und stillen Gr$\"{o}{\beta}$e, die sonst in der Natur verborgen bleibe. Nach Winckelmann sollte der K$\"{u}$nstler die griechischen Vorbilder wie Apollo von Belvedere und Laokoon nachahmen, um das wesentlich Sch$\"{o}$ne zu erlernen. Falconet $\"{u}$bte eine scharfe Kritik gegen die Meinung Winckelmanns und die Diletantismus in der Kunstkritik aus. Selbst als Theoretiker warf er vor, dass die Klassizisten um ihr dogmatisch struktuiertes Lehrgeb$\"{a}$ude willen alle andere Eigenschaften der Kunst sowie Kunstwerke $\"{u}$bersahen und ihre Apotheose der klassischen Kunst auf die Entwicklung der Kunst eher als Hindernis wirkte. Im besonderen Hinblick auf den Ausdruck der menschlichen Leidenschaft distanzierte Falconet sich von der Lehrmeinung des Klassizismus, n$\"{a}$mlich Zur$\"{u}$ckhaltung derselben Leidenschaft wegen der idealen Sch$\"{o}$nheit. Vor dem Laokoon teilten Falconet und die Klassizisten ihre Meinung un$\"{u}$berbr$\"{u}$ckbar voneinander. F$\"{u}$r diese war der Laokoon die Repr$\"{a}$sentation des erhabenen Menschen, der leidet, ohne seinen Affekt zu enth$\"{u}$llen. Im Gegensatz sah Falconet in demselben Werk einen erlungenen Ausdruck des menschlichen Gef$\"{u}$hls. Sein Deb$\"{u}$twerk ist also die Visualisierung seiner Kunstauffassung. Unter Einfluss Pierre Pugets sowie seines Lehrers Jean-Baptiste Lemoyne bem$\"{u}$ht er sich darum, den Ausdruck der menschlichen Leidenschaft durch kunsttechnische Leistung und wissenschaftliche Forschung zu erstarken. Daraus ergab sich die expressive Ausdrucksform des Affekts. In diesem Fall verband sich Falconet mit der Tradition des barocken Kunstwollens, aber er ging einen Schritt weiter, indem er die Ausdrucksweise noch realistischer und lebendiger zu machen vermag. Faconet war vielleicht der einzige K$\"{u}$nstler, der Vorteile det barocken Kunst ausnut zen konnte, ohne dabei die klassische und realistische Formensprache zu verlieren. Dutch sein Werk und seine theoretischen Schriften er$\"{o}$ffnete er neue Prinzipien sowohl f$\"{u}$r die Kunstpraxis als auch f$\"{u}$r die Kunstkritik, deren wesentliche Sinn jedoch erst in der Romantik anerkannt wurde.