• 제목/요약/키워드: DC21

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Identification of DC21 as a Novel Target Gene Counter-regulated by IL-12 and IL-4

  • Kong, Kyoung-Ah;Jang, Ji-Young;Lee, Choong-Eun
    • BMB Reports
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    • 제35권6호
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    • pp.623-628
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    • 2002
  • The Th1 vs. Th2 balance is critical for the maintenance of immune homeostasis. Therefore, the genes that are selectively-regulated by the Th1 and Th2 cytokines are likely to play an important role in the Th1 and Th2 immune responses. In order to search for and identify the novel target genes that are differentially regulated by the Th1/Th2 cytokines, the human PBMC mRNAs differentially expressed upon the stimulation with IL-4 or IL-12, were screened by employing the differential display-polymerase chain reaction. Among a number of clones selected, DC21 was identified as a novel target gene that is regulated by IL-4 and IL-12. The DC21 gene expression was up-regulated either by IL-4 or IL-12, yet counter-regulated by co-treatment with IL-4 and IL-12. DC21 is a dendritic cell protein with an unknown function. The sequence analysis and conserved-domain search revealed that it has two AU-rich motifs in the 3'UTR, which is a target site for the regulation of mRNA stability by cytokines, and that it belongs to the N-acetyltransferase family. The induction of DC21 by IL-12 peaked around 8-12 h, and lasted until 24 h. LY294002 and SB203580 significantly suppressed the IL-12-induced DC21 gene expression, which implies that PI3K and p38/JNK are involved in the IL-12 signal transduction pathway that leads to the DC21 expression. Furthermore, tissue blot data indicated that DC21 is highly expressed in tissues with specialized-resident macrophages, such as the lung, liver, kidney, and placenta. Together, these data suggest a possible role for DC21 in the differentiation and maturation of dendritic cells regulated by IL-4 and IL-12.

Screening and Characterization of Pro biotic Lactic Acid Bacteria Isolated from Korean Fermented Foods

  • Lim, Sung-Mee;Im, Dong-Soon
    • Journal of Microbiology and Biotechnology
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    • 제19권2호
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    • pp.178-186
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    • 2009
  • To examine their potential as probiotics, acid and bile tolerance, antibiotics resistance, adhesion capacity to Caco-2 and HT-29, and antibacterial activity, of LAB isolated from Korean fermented foods such. as dongchimi, kimchi, Meju, and doenjang were assayed against foodborne pathogenic bacteria. DC 55, DC 136, DC 222, KC 21, KC 24, KC 34, KC 43, KC 117, MJ 54, MJ 301, SP 33, and SP 170 strains were resistant to acid and bile conditions. In particular, DC 55, DC 136, KC 24, KC 43, and MJ 301 strains were highly resistant to higher than 20 ${\mu}g/ml$ concentrations of vancomycin, streptomycin sulfate, or amoxicillin, whereas, DC 222, KC 21, KC 34, KC 117, MJ 54, and SP 33 strains were susceptible to lower than 2 ${\mu}g/ml$ concentrations of those antibiotics. The adhesion to HT-29 and Caco-2 cells varied with the strains tested in a strain-dependent manner. The highest level of adhesion was observed with DC 55, KC 21, KC 24, and MJ 301 strains, having higher than 50% of adhesion to HT-29 or Caco-2 cells. In addition, Staphylococcus aureus was the most sensitive to KC 21, showing an inhibition of about 70%, and the antibacterial activity of KC 21 against S. aureus resulted most likely from both organic acids and bacteriocin. Based on its phenotypic characteristics and utilization of various sugars, the KC 21 strain was identified as Lactobacillus plantarum.

자기적 아크소호 기법을 이용한 직류 차단기 개발 (Development of DC Circuit Breaker using Magnet Arc Extinguisher)

  • 이성민;김효성
    • 전력전자학회논문지
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    • 제17권1호
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    • pp.21-26
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    • 2012
  • In recent years, DC distribution systems are becoming hot issue due to the increase in digital loads and DC generation systems according to the expansion of renewable energy technologies. However, removing the fault current in DC grids is comparably difficult since the current in DC grids has no zero-crossing point like in AC grids. Thus, developing dedicated DC circuit breakers for DC grids is necessary to get safety for people and electrical facilities. This paper proposes magnet arc extinguishing method to develop a 300[$V_{DC}$]/10[A] DC circuit breaker. The performance of the proposed DC circuit breaker was verified by an experimental circuit breaker test system built in this research.

Low price Fuel Cell Inverter System for 3[KW] Residential Power

  • Kwon, Soon-Kurl
    • 조명전기설비학회논문지
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    • 제21권4호
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    • pp.61-72
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    • 2007
  • This study proposed a high efficiency DC-DC converter with a new current doubler rectifier for fuel-cell systems for use with the Nexa(310-0027) PEMFC from the Ballard Co. The proposed high efficiency DC-DC converter for the fuel-cell system generated ZVS by applying partial resonance and using a phase shift PWM control method. Constantly switching frequency, loss of switching, peak current, and peak voltage were reduced by this system. In addition to this system, two inductors were attached to a rectifier circuit allowing it to be able to provide the direct current(DC) and DC voltage safely to a load with reduced ripple components. Also, by using the newly proposed current doubler rectifier, the high frequency DC-DC converter for the fuel cell system was capable of reaching a highest efficiency of 92[%] as compared to 88.3[%] efficiency in previous results, which means that efficiency increased 3.7[%]. The overall results were confirmed by a simulation and laboratory experiment.

입·출력 전압 변동을 고려한 3상 인터리브드 양방향 DC-DC컨버터의 전류리플 저감 기법 (Current Ripple Reduction Method of 3-phase Interleaved Bidirectional DC-DC Converter with the Consideration of Input and Output Voltage Variation)

  • 선다운;정재헌;노의철;정규범
    • 전력전자학회논문지
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    • 제21권5호
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    • pp.427-433
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    • 2016
  • This paper proposes a new method for the current ripple reduction of a three-phase interleaved bidirectional DC-DC converter. Usually, the three-phase interleaved bidirectional DC-DC converter is used for battery charging and discharging to reduce battery current ripple. In V2G application, a PWM AC-DC converter is used to connect the AC power grid and three-phase interleaved bidirectional DC-DC converter for battery charging and discharging. The magnitude of DC link voltage affects the battery current ripple magnitude. Therefore, the magnitude of the battery ripple current is analyzed with variations of battery and DC link voltages. The ripple current magnitude is found to be minimized by controlling the DC link voltage. Simulation and experimental results show the usefulness of the proposed method.

주파수 변조 방식 3상 인터리브드 양방향 DC-DC 컨버터의 과도상태 과전류를 최소화하기 위한 스위칭 기법 (A Switching Method for Minimizing the Over Current in Transient Response of 3-phase Interleaved Bidirectional DC-DC Converter with Frequency Modulation)

  • 배종우;정혜수;정재헌;노의철
    • 전력전자학회논문지
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    • 제21권6호
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    • pp.530-537
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    • 2016
  • This work deals with a switching method for minimizing overcurrent in a three-phase interleaved bidirectional DC-DC converter with frequency modulation. Generally, a three-phase interleaved DC-DC converter is used to reduce a current ripple component. The combined operation of three-phase and two-phase converters can significantly reduce the ripple component. However, the conventional PWM method cannot solve severe overcurrent during phase transfer or frequency variation for power control. To overcome this problem, this work proposes a new PWM switching method. A 3 kW DC-DC power converter is designed and implemented, and the converter is operated in discontinuous current mode with varying switching frequencies for power control. Simulation and experimental results show the validity of the proposed switching method. The proposed switching method can be widely used in the field of current ripple reduction for three-phase interleaved bidirectional DC-DC converters.

Antimicrobial Effects of a Hexapetide KCM21 against Pseudomonas syringae pv. tomato DC3000 and Clavibacter michiganensis subsp. michiganensis

  • Choi, Jeahyuk;Baek, Kwang-Hyun;Moon, Eunpyo
    • The Plant Pathology Journal
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    • 제30권3호
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    • pp.245-253
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    • 2014
  • Antimicrobial peptides (AMPs) are small but effective cationic peptides with variable length. In previous study, four hexapeptides were identified that showed antimicrobial activities against various phytopathogenic bacteria. KCM21, the most effective antimicrobial peptide, was selected for further analysis to understand its modes of action by monitoring inhibitory effects of various cations, time-dependent antimicrobial kinetics, and observing cell disruption by electron microscopy. The effects of KCM21 on Gram-negative strain, Pseudomonas syringae pv. tomato DC3000 and Gram-positive strain, Clavibacter michiganensis subsp. michiganensis were compared. Treatment with divalent cations such as $Ca^{2+}$ and $Mg^{2+}$ inhibited the bactericidal activities of KCM21 significantly against P. syringae pv. tomato DC3000. The bactericidal kinetic study showed that KCM21 killed both bacteria rapidly and the process was faster against C. michiganensis subsp. michiganensis. The electron microscopic analysis revealed that KCM21 induced the formation of micelles and blebs on the surface of P. syringae pv. tomato DC3000 cells, while it caused cell rupture against C. michiganensis subsp. michiganensis cells. The outer membrane alteration and higher sensitivity to $Ca^{2+}$ suggest that KCM21 interact with the outer membrane of P. syringae pv. tomato DC3000 cells during the process of killing, but not with C. michiganensis subsp. michiganensis cells that lack outer membrane. Considering that both strains had similar sensitivity to KCM21 in LB medium, outer membrane could not be the main target of KCM21, instead common compartments such as cytoplasmic membrane or internal macromolecules might be a possible target(s) of KCM21.

3[kW]급 연료전지용 전력변환장치의 개발 (Development of 3.0[kW]class Fuel Cell Power Conversion System)

  • 서기영
    • 조명전기설비학회논문지
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    • 제21권2호
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    • pp.54-63
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    • 2007
  • 최근 저전압 대전류 출력 특성을 갖는 연료전지를 위한 새로운 발전시스템으로 주목받고 있다. 연료전지 발전시스템에서는 DC-DC 승압용 컨버터와 DC-AC 인버터가 필요하다. 그러므로 본 논문에서는 연료전지의 전압을 $380[V_{DC}]$로 승압하기 위한 절연형 DC-DC 컨버터와 단상 $220[V_{AC}]$로 변환하기 위한 LC필터를 가진 PWM 인버터로 구성된 전력변환장치를 제안하였다. 특히 제안한 고주파 절연형 ZVZCS PWM DC-DC 컨버터는 환류 다이오드를 포함한 탭부 인덕터 필터를 이용하여 순환 전류를 저감시켰으며, 스위치 및 변압기의 턴-온, 턴-오프시에 오버슈트 전압이나 과도현상이 발생하지 않는다. 그리고 넓은 출력 전압 조정에도 효율을 $93{\sim}97[%]$정도 얻을 수 있으며, 출력 부하전류의 변화에 대해 거의 일정한 출력 전압 특성을 가졌다.

비접촉 전원장치에 적용한 LCC형 고주파 공진 DC/DC 컨버터에 관한 연구 (A Study on the LCC Type High Frequency DC/DC Converter for Contactless Power Supply System)

  • 김동희;황계호
    • 조명전기설비학회논문지
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    • 제21권6호
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    • pp.55-64
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    • 2007
  • 본 논문은 부하직렬 고주파 공진 DC/DC 컨버터에 가변 가능한 공진커패시터를 병렬로 삽입한 LCC형 고주파 공진 DC/DC 컨버터의 특성과 설계 예를 나타내고 있으며, 또한 턴-오프시 스위칭 손실을 줄이기 위해 ZVS를 가지는 소프트 스위칭을 사용하였다. 제안 컨버터는 PFM(Pulse Frequency Modulation) 스위칭 패턴을 사용하여 동작되며, PFM 제어에 의해 제안 회로의 출력전압을 제어하였다. 병렬커패시터의 커패시턴스의 변화에 따라, DC/DC 컨버터의 분석은 일반적으로 무차원화 파라미터를 사용하여 나타내었고, 회로 동작 특성은 스위칭 주파수와 파라미터로 행하였다. 또한 본 논문은 특성평가에 의해 제안 DC/DC 컨버터의 동작 특성과 회로 설계 방법을 제시하였다. 더욱이 본 논문은 실험을 통해 이론 분석의 정당성을 입증하였다. 향후 제안한 DC/DC 컨버터는 반도체 및 FPD의 클린룸에 선형이동 시스템의 비접촉 전원장치에 적용 가능하다고 생각된다.

DC23, a Triazolothione Resorcinol Analogue, Is Extensively Metabolized to Glucuronide Conjugates in Human Liver Microsomes

  • Shon, Jong Cheol;Joo, Jeongmin;Lee, Taeho;Kim, Nam Doo;Liu, Kwang-Hyeon
    • Mass Spectrometry Letters
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    • 제9권1호
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    • pp.24-29
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    • 2018
  • DC23, a triazolothione resorcinol analogue, is known to inhibit heat shock protein 90 and pyruvate dehydrogenase kinase which are up-regulated in cancer and diabetes, respectively. This study was performed to elucidate the metabolism of DC23 in human liver microsomes (HLMs). HLMs incubated with DC23 in the presence of uridine 5'-diphosphoglucuronic acid (UDPGA) and/or ${\beta}$-nicotinamide adenine dinucleotide phosphate (NADPH) resulted in the formation of four metabolites, M1-M4. M1 was identified as DC23-N-Oxide, on the basis of LC-MS/MS analysis. DC23 was further metabolized to its glucuronide conjugates (M2, M3, and M4). In vitro metabolic stability studies conducted with DC23 in HLMs revealed significant glucuronide conjugation with a $t_{1/2}$ value of 1.3 min. The inhibitory potency of DC23 on five human cytochrome P450s was also investigated in HLMs. In these experiments, DC23 inhibited CYP2C9-mediated tolbutamide hydroxylase activity with an $IC_{50}$ value of $8.7{\mu}M$, which could have implications for drug interactions.