• Title/Summary/Keyword: DC21

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Identification of DC21 as a Novel Target Gene Counter-regulated by IL-12 and IL-4

  • Kong, Kyoung-Ah;Jang, Ji-Young;Lee, Choong-Eun
    • BMB Reports
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    • v.35 no.6
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    • pp.623-628
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    • 2002
  • The Th1 vs. Th2 balance is critical for the maintenance of immune homeostasis. Therefore, the genes that are selectively-regulated by the Th1 and Th2 cytokines are likely to play an important role in the Th1 and Th2 immune responses. In order to search for and identify the novel target genes that are differentially regulated by the Th1/Th2 cytokines, the human PBMC mRNAs differentially expressed upon the stimulation with IL-4 or IL-12, were screened by employing the differential display-polymerase chain reaction. Among a number of clones selected, DC21 was identified as a novel target gene that is regulated by IL-4 and IL-12. The DC21 gene expression was up-regulated either by IL-4 or IL-12, yet counter-regulated by co-treatment with IL-4 and IL-12. DC21 is a dendritic cell protein with an unknown function. The sequence analysis and conserved-domain search revealed that it has two AU-rich motifs in the 3'UTR, which is a target site for the regulation of mRNA stability by cytokines, and that it belongs to the N-acetyltransferase family. The induction of DC21 by IL-12 peaked around 8-12 h, and lasted until 24 h. LY294002 and SB203580 significantly suppressed the IL-12-induced DC21 gene expression, which implies that PI3K and p38/JNK are involved in the IL-12 signal transduction pathway that leads to the DC21 expression. Furthermore, tissue blot data indicated that DC21 is highly expressed in tissues with specialized-resident macrophages, such as the lung, liver, kidney, and placenta. Together, these data suggest a possible role for DC21 in the differentiation and maturation of dendritic cells regulated by IL-4 and IL-12.

Screening and Characterization of Pro biotic Lactic Acid Bacteria Isolated from Korean Fermented Foods

  • Lim, Sung-Mee;Im, Dong-Soon
    • Journal of Microbiology and Biotechnology
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    • v.19 no.2
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    • pp.178-186
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    • 2009
  • To examine their potential as probiotics, acid and bile tolerance, antibiotics resistance, adhesion capacity to Caco-2 and HT-29, and antibacterial activity, of LAB isolated from Korean fermented foods such. as dongchimi, kimchi, Meju, and doenjang were assayed against foodborne pathogenic bacteria. DC 55, DC 136, DC 222, KC 21, KC 24, KC 34, KC 43, KC 117, MJ 54, MJ 301, SP 33, and SP 170 strains were resistant to acid and bile conditions. In particular, DC 55, DC 136, KC 24, KC 43, and MJ 301 strains were highly resistant to higher than 20 ${\mu}g/ml$ concentrations of vancomycin, streptomycin sulfate, or amoxicillin, whereas, DC 222, KC 21, KC 34, KC 117, MJ 54, and SP 33 strains were susceptible to lower than 2 ${\mu}g/ml$ concentrations of those antibiotics. The adhesion to HT-29 and Caco-2 cells varied with the strains tested in a strain-dependent manner. The highest level of adhesion was observed with DC 55, KC 21, KC 24, and MJ 301 strains, having higher than 50% of adhesion to HT-29 or Caco-2 cells. In addition, Staphylococcus aureus was the most sensitive to KC 21, showing an inhibition of about 70%, and the antibacterial activity of KC 21 against S. aureus resulted most likely from both organic acids and bacteriocin. Based on its phenotypic characteristics and utilization of various sugars, the KC 21 strain was identified as Lactobacillus plantarum.

Development of DC Circuit Breaker using Magnet Arc Extinguisher (자기적 아크소호 기법을 이용한 직류 차단기 개발)

  • Lee, Sung-Min;Kim, Hyo-Sung
    • The Transactions of the Korean Institute of Power Electronics
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    • v.17 no.1
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    • pp.21-26
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    • 2012
  • In recent years, DC distribution systems are becoming hot issue due to the increase in digital loads and DC generation systems according to the expansion of renewable energy technologies. However, removing the fault current in DC grids is comparably difficult since the current in DC grids has no zero-crossing point like in AC grids. Thus, developing dedicated DC circuit breakers for DC grids is necessary to get safety for people and electrical facilities. This paper proposes magnet arc extinguishing method to develop a 300[$V_{DC}$]/10[A] DC circuit breaker. The performance of the proposed DC circuit breaker was verified by an experimental circuit breaker test system built in this research.

Low price Fuel Cell Inverter System for 3[KW] Residential Power

  • Kwon, Soon-Kurl
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.21 no.4
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    • pp.61-72
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    • 2007
  • This study proposed a high efficiency DC-DC converter with a new current doubler rectifier for fuel-cell systems for use with the Nexa(310-0027) PEMFC from the Ballard Co. The proposed high efficiency DC-DC converter for the fuel-cell system generated ZVS by applying partial resonance and using a phase shift PWM control method. Constantly switching frequency, loss of switching, peak current, and peak voltage were reduced by this system. In addition to this system, two inductors were attached to a rectifier circuit allowing it to be able to provide the direct current(DC) and DC voltage safely to a load with reduced ripple components. Also, by using the newly proposed current doubler rectifier, the high frequency DC-DC converter for the fuel cell system was capable of reaching a highest efficiency of 92[%] as compared to 88.3[%] efficiency in previous results, which means that efficiency increased 3.7[%]. The overall results were confirmed by a simulation and laboratory experiment.

Current Ripple Reduction Method of 3-phase Interleaved Bidirectional DC-DC Converter with the Consideration of Input and Output Voltage Variation (입·출력 전압 변동을 고려한 3상 인터리브드 양방향 DC-DC컨버터의 전류리플 저감 기법)

  • Sun, Daun;Jung, Jae-Hun;Nho, Eui-Cheol;Joung, Gyu-Bum
    • The Transactions of the Korean Institute of Power Electronics
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    • v.21 no.5
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    • pp.427-433
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    • 2016
  • This paper proposes a new method for the current ripple reduction of a three-phase interleaved bidirectional DC-DC converter. Usually, the three-phase interleaved bidirectional DC-DC converter is used for battery charging and discharging to reduce battery current ripple. In V2G application, a PWM AC-DC converter is used to connect the AC power grid and three-phase interleaved bidirectional DC-DC converter for battery charging and discharging. The magnitude of DC link voltage affects the battery current ripple magnitude. Therefore, the magnitude of the battery ripple current is analyzed with variations of battery and DC link voltages. The ripple current magnitude is found to be minimized by controlling the DC link voltage. Simulation and experimental results show the usefulness of the proposed method.

A Switching Method for Minimizing the Over Current in Transient Response of 3-phase Interleaved Bidirectional DC-DC Converter with Frequency Modulation (주파수 변조 방식 3상 인터리브드 양방향 DC-DC 컨버터의 과도상태 과전류를 최소화하기 위한 스위칭 기법)

  • Bae, Jongwoo;Jeong, Hyesoo;Jung, Jae-Hun;Nho, Eui-Cheol
    • The Transactions of the Korean Institute of Power Electronics
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    • v.21 no.6
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    • pp.530-537
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    • 2016
  • This work deals with a switching method for minimizing overcurrent in a three-phase interleaved bidirectional DC-DC converter with frequency modulation. Generally, a three-phase interleaved DC-DC converter is used to reduce a current ripple component. The combined operation of three-phase and two-phase converters can significantly reduce the ripple component. However, the conventional PWM method cannot solve severe overcurrent during phase transfer or frequency variation for power control. To overcome this problem, this work proposes a new PWM switching method. A 3 kW DC-DC power converter is designed and implemented, and the converter is operated in discontinuous current mode with varying switching frequencies for power control. Simulation and experimental results show the validity of the proposed switching method. The proposed switching method can be widely used in the field of current ripple reduction for three-phase interleaved bidirectional DC-DC converters.

Antimicrobial Effects of a Hexapetide KCM21 against Pseudomonas syringae pv. tomato DC3000 and Clavibacter michiganensis subsp. michiganensis

  • Choi, Jeahyuk;Baek, Kwang-Hyun;Moon, Eunpyo
    • The Plant Pathology Journal
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    • v.30 no.3
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    • pp.245-253
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    • 2014
  • Antimicrobial peptides (AMPs) are small but effective cationic peptides with variable length. In previous study, four hexapeptides were identified that showed antimicrobial activities against various phytopathogenic bacteria. KCM21, the most effective antimicrobial peptide, was selected for further analysis to understand its modes of action by monitoring inhibitory effects of various cations, time-dependent antimicrobial kinetics, and observing cell disruption by electron microscopy. The effects of KCM21 on Gram-negative strain, Pseudomonas syringae pv. tomato DC3000 and Gram-positive strain, Clavibacter michiganensis subsp. michiganensis were compared. Treatment with divalent cations such as $Ca^{2+}$ and $Mg^{2+}$ inhibited the bactericidal activities of KCM21 significantly against P. syringae pv. tomato DC3000. The bactericidal kinetic study showed that KCM21 killed both bacteria rapidly and the process was faster against C. michiganensis subsp. michiganensis. The electron microscopic analysis revealed that KCM21 induced the formation of micelles and blebs on the surface of P. syringae pv. tomato DC3000 cells, while it caused cell rupture against C. michiganensis subsp. michiganensis cells. The outer membrane alteration and higher sensitivity to $Ca^{2+}$ suggest that KCM21 interact with the outer membrane of P. syringae pv. tomato DC3000 cells during the process of killing, but not with C. michiganensis subsp. michiganensis cells that lack outer membrane. Considering that both strains had similar sensitivity to KCM21 in LB medium, outer membrane could not be the main target of KCM21, instead common compartments such as cytoplasmic membrane or internal macromolecules might be a possible target(s) of KCM21.

Development of 3.0[kW]class Fuel Cell Power Conversion System (3[kW]급 연료전지용 전력변환장치의 개발)

  • Suh, Ki-Young
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.21 no.2
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    • pp.54-63
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    • 2007
  • Recently, a fuel cell with low voltage and high current output characteristics is remarkable for new generation system. It needs both a DC-DC step-up converter and DC-AC inverter to be used in fuel cell generation system. Therefor, this paper, consists of an isolated DC-DC converter to boost the fuel cell voltage $380[V_{DC}]$ and a PWM inverter with LC filter to convent the DC voltage to single-phase $220[V_{AC}]$. Expressly, a tapped inductor filter with freewheeling diode is newly implemented in the output filter of the proposed high frequency isolated ZVZCS PWM DC-DC converter to suppress circulating current under the wide output voltage regulation range, thus to eliminate the switching and transformer turn-on/off over-short voltage or transient phenomena. Besides the efficiency of 93-97[%]is obtained over the wide output voltage regulation ranges and load variations.

A Study on the LCC Type High Frequency DC/DC Converter for Contactless Power Supply System (비접촉 전원장치에 적용한 LCC형 고주파 공진 DC/DC 컨버터에 관한 연구)

  • Kim, Dong-Hee;Hwang, Gye-Ho
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.21 no.6
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    • pp.55-64
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    • 2007
  • This paper represents characteristics and design example of series loaded LCC type high frequency resonant DC-DC converter with variable parallel capacitor in the secondary side of inductive power transformer. In this converter, ZVS(zero voltage switching) technique is applied to reduce turn-off switching losses, and the applied converter used the PFM switching pattern to control output voltage. The operating characteristics of the proposed converter is analyzed using nomalized parameter such as switching frequency and load factor with varing the secondary parallel resonant capacitor. The results of analysis show the operating characteristics and design method of the proposed converter using characteristic values. And the proposed converter can be applied for the contactless power supply with linear transfer system such as dean room facilities of semiconductor and Flat Panel Display.

DC23, a Triazolothione Resorcinol Analogue, Is Extensively Metabolized to Glucuronide Conjugates in Human Liver Microsomes

  • Shon, Jong Cheol;Joo, Jeongmin;Lee, Taeho;Kim, Nam Doo;Liu, Kwang-Hyeon
    • Mass Spectrometry Letters
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    • v.9 no.1
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    • pp.24-29
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    • 2018
  • DC23, a triazolothione resorcinol analogue, is known to inhibit heat shock protein 90 and pyruvate dehydrogenase kinase which are up-regulated in cancer and diabetes, respectively. This study was performed to elucidate the metabolism of DC23 in human liver microsomes (HLMs). HLMs incubated with DC23 in the presence of uridine 5'-diphosphoglucuronic acid (UDPGA) and/or ${\beta}$-nicotinamide adenine dinucleotide phosphate (NADPH) resulted in the formation of four metabolites, M1-M4. M1 was identified as DC23-N-Oxide, on the basis of LC-MS/MS analysis. DC23 was further metabolized to its glucuronide conjugates (M2, M3, and M4). In vitro metabolic stability studies conducted with DC23 in HLMs revealed significant glucuronide conjugation with a $t_{1/2}$ value of 1.3 min. The inhibitory potency of DC23 on five human cytochrome P450s was also investigated in HLMs. In these experiments, DC23 inhibited CYP2C9-mediated tolbutamide hydroxylase activity with an $IC_{50}$ value of $8.7{\mu}M$, which could have implications for drug interactions.