• Title/Summary/Keyword: D3-28S

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Phylogenetic Analysis of Ruminant Theileria spp. from China Based on 28S Ribosomal RNA Gene

  • Gou, Huitian;Guan, Guiquan;Ma, Miling;Liu, Aihong;Liu, Zhijie;Xu, Zongke;Ren, Qiaoyun;Li, Youquan;Yang, Jifei;Chen, Ze;Yin, Hong;Luo, Jianxun
    • Parasites, Hosts and Diseases
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    • v.51 no.5
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    • pp.511-517
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    • 2013
  • Species identification using DNA sequences is the basis for DNA taxonomy. In this study, we sequenced the ribosomal large-subunit RNA gene sequences (3,037-3,061 bp) in length of 13 Chinese Theileria stocks that were infective to cattle and sheep. The complete 28S rRNA gene is relatively difficult to amplify and its conserved region is not important for phylogenetic study. Therefore, we selected the D2-D3 region from the complete 28S rRNA sequences for phylogenetic analysis. Our analyses of 28S rRNA gene sequences showed that the 28S rRNA was useful as a phylogenetic marker for analyzing the relationships among Theileria spp. in ruminants. In addition, the D2-D3 region was a short segment that could be used instead of the whole 28S rRNA sequence during the phylogenetic analysis of Theileria, and it may be an ideal DNA barcode.

Effects of Essential Oils Supplementation on Growth Performance, IgG Concentration and Fecal Noxious Gas Concentration of Weaned Pigs

  • Cho, J.H.;Chen, Y.J.;Min, B.J.;Kim, H.J.;Kwon, O.S.;Shon, K.S.;Kim, I.H.;Kim, S.J.;Asamer, A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.1
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    • pp.80-85
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    • 2006
  • Ninety six crossbred pigs (Landrace${\times}$Yorkshire${\times}$Duroc) were used to determine the effects of essential oils (Fresta F $Conc^{(R)}$) supplementation on growth performance, immune response and fecal noxious gas of weaned pigs. Treatments were 1) NC (negative control; basal diet without antibiotics), 2) PC [positive control; basal diet+CSP (CTC+Sulfathiazole+Penicillin) 0.1%], 3) NCF (basal diet+Fresta F $Conc^{(R)}$ 0.03%) and 4) PCF [basal diet+CSP (CTC+Sulfathiazole+Penicillin) 0.1%+Fresta F $Conc^{(R)}$ 0.02%]. From d 0 to 14, ADFI was increased in pigs fed PCF diet (p<0.05). From d 14 to 28, pigs fed PCF diet had greater ADG and ADFI than pigs fed NC diet (p<0.05). From d 28 to 49, ADG and ADFI in pigs fed PCF diet were higher than in pigs fed NC diet (p<0.05). Through the entire experimental period, ADG and ADFI in pigs fed PCF diet were the highest compared to pigs fed NC and PC diets (p<0.05). There was no significant difference in fecal consistency score among the treatments (p>0.05). No statistical differences (p>0.05) were found in red blood cells (RBC) counts, white blood cells (WBC) counts, lymphocyte counts, total protein and albumin. Serum IgG concentration of PCF treatment was greater than that of other treatments (p<0.05). From d 0 to 14, there was no significant difference in digestibility of dry matter and nitrogen among the treatments (p>0.05). From d 14 to 28, digestibility of dry matter in pigs fed PC, NCF and PCF diets was higher than that of pigs fed NC diet (p<0.05) and treatments with added essential oils were higher than other diets on digestibility of nitrogen (p<0.05). Also, from d 28 to 49, digestibility of nitrogen in pigs fed PCF diet was the highest among others (p<0.05). On d 14 and 28, no statistical differences (p>0.05) were found in volatile fatty acid (VFA), ammonia nitrogen ($NH_3$-N) and hydrogen sulfide ($H_2S$) concentrations among treatments. On d 49, there was no significant difference in VFA concentration among the treatments (p>0.05). $NH_3$-N concentration in pigs fed PCF diet was lower than in pigs fed other diets (p<0.05). $H_2S$ concentration in pigs fed diets with added essential oils was lower than others. In conclusion, the results suggest that the dietary addition of essential oils and antibiotics into diets for weanling pigs improved growth performance, IgG concentration and nitrogen digestibility and decreased noxious gas concentration. Essential oils can be used to partly replace antibiotics in diets for weaned pigs without negative affects on growth performance.

The Effect of Vitamin D and Calcium on Cognitive Function and Depression in the Elderly Living in a City

  • Lee, Yu-Jin;Kim, Yun-Su
    • Research in Community and Public Health Nursing
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    • v.28 no.3
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    • pp.251-259
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    • 2017
  • Purpose: This study aims to examine the influence of vitamin D and calcium on depression and cognitive function of the elderly living alone in a city. Methods: The participants were registered in eight senior centers in S city and they had lived alone. Data were collected between November 28, 2014 and March 7, 2015. A total of 155 people participated in data collection to measure the serum vitamin D, the serum calcium, depression, and cognitive function. The data were analyzed with t-test, ANOVA, Pearson's correlation and multiple regression analysis. Results: There were significant differences in depression according to gender and perceptions of health status. Depression correlated significantly with the serum calcium and perceptions of health status, and a stepwise regression analysis showed that the perceptions of health status were significant. There were significant differences in cognitive function according to education level and age. Cognitive function correlated significantly with the serum vitamin D and a stepwise regression analysis showed that education level and age were significant. Conclusion: Consequently, elderly people with poor perceptions of their health status need a depressive intervention program and those with a higher age and lower level of education need a cognitive function intervention program.

A S/C/X-Band GaN Low Noise Amplifier MMIC (S/C/X-대역 GaN 저잡음 증폭기 MMIC)

  • Han, Jang-Hoon;Kim, Jeong-Geun
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.28 no.5
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    • pp.430-433
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    • 2017
  • This paper presents a S/C/X-band LNA MMIC with resistive feedback structure in 0.25 um GaN HEMT process. The GaN devices have advantages as a high output power device having high breakdown voltage, energy band gap and stability at high temperature. Since the receiver using the GaN device with high linearity can be implemented without a limiter, the noise figure of the receiver can be improved and the size of receiver module can be reduced. The proposed GaN LNA MMIC based on 0.25 um GaN HEMT device is achieved the gain of > 15 dB, the noise figure of < 3 dB, the input return loss of > 13 dB, and the output return loss of > 8 dB in the S/C/X-band. The current consumption of GaN LNA MMIC is 70 mA with the drain voltage 20 V and the gate voltage -3 V.

LUMINOSITY PROFILES OF dE AND dS0 GALAXIES IN THE VIRGO CLUSTER

  • Kim, Kyoo-Hyun;Lee, Kyung-Hoon;Ann, Hong-Bae
    • Journal of The Korean Astronomical Society
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    • v.39 no.3
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    • pp.57-71
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    • 2006
  • We investigated the structural parameters of a sample of 30 dwarf galaxies(15 dEs and 15 dS0s) in the Virgo Cluster using i-band images from the Sloan Digital Sky Survey Data Release 4. Among 28 galaxies for which surface brightness profiles were derived from ellipse fittings, 23 galaxies had a single component that was adequately described by a generalized $S\acute{e}rsic$ function with a shape parameter ranging from n=0.5 to 2, while 5 galaxies(2 dEs and 3 dS0s) had bulge and disk components that were fitted by a generalized $S\acute{e}rsic$ function and an exponential function, respectively. Since the majority of dwarf galaxies in the present sample had a single component, it seems likely that genuine dS0 galaxies that have disk and bulge components are quite rare in the Virgo Cluster. The similarity in structural parameters of genuine dS0 galaxies in the Virgo Cluster with those of Magellanic-type galaxies implies that the progenitors of dwarf lenticular galaxies in the Virgo Cluster were most likely Magellanic-type galaxies if dS0s are harassed late-type spirals.

The Study of Removal of Organic Matter by the Rockworm, Marphysa sanguinea (바위털갯지렁이, Marphysa sanguinea에 의한 유기물 제거에 관한 연구)

  • Baek, Ami;Lee, Kyoung Seon
    • Journal of Marine Life Science
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    • v.3 no.1
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    • pp.38-43
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    • 2018
  • The polychaetes are the dominant species in the benthic communities, and play an important role in improving the sediment quality through the action of ingesting organic materials. We evaluated the improvement ability of rockworm Marphysa sanguinea for the removal of organic matter. We used the three types of sediment (S1: coarse sand, S2: fine sand, S3: muddy sand), and analyzed TOC of seawater and sediment. Rockworm was effective in eliminating organic matter in the three types of sediment and the larger the particle, the higher the removal rate. Removal ability of rockworm for organic matter in sediment were calculated with 3.9856 ppm g-1d-1 in coarse sand, 2.8021 ppm g-1d-1 in fine sand, and 28.1142 ppm g-1d-1 in muddy sand. The results show that rockworm contributes to the removal of organic matter in the various sediment types.

Effects of Sodium Sulfite and Extrusion on the Nutritional Value of Soybean Meal in Piglets Weaned at 21 Days

  • Piao, X.S.;Jin, J.;Kim, J.D.;Kim, J.H.;Sohn, K.S.;Hyun, Y.;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.7
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    • pp.974-979
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    • 2000
  • A total of 80 weaned piglets (Landrace $\times$ Yorkshire $\times$ Large White) were used in a 28-day growth assay to detennine the optimal inclusion level of sodium sulfite ($Na_{2}SO_{3}$) as an extrusion enhancer of soybean meal for nursery piglets. piglets (21 d of age, 6.04 kg of BW) were grouped into 4 treatments in a completely randomized block design. Treatments were: 1) Extruded SBM (Control), 2) Extruded SBM with 0.5% $Na_{2}SO_{3}$ (0.5 ESBM), 3) Extruded SBM with 1.0% $Na_{2}SO_{3}$ (1.0 ESBM) and 4) Extruded SBM with 1.5% $Na_{2}SO_{3}$ (1.5 ESBM). Each treatment has 4 replicates of 5 heads per pen. In phase I (d 0 to 14), diets supplied 3,400 kcal ME/kg, 23% crude protein, 1.65% lysine, 0.50% methionine, 0.9% Ca and 0.8% P. Phase II (d 14 to 28) diets contained 3,300 kcal ME/kg, 21% crude protein, 1.45% lysine, 0.45% methionine, 0.9% Ca and 0.8% P. For d 0 to 14, piglets fed 1.5 ESBM had greater ADG, ADFI and FCR compared to piglets fed control and 0.5 ESBM diet. ADG was significantly higher in piglets fed 1.5 ESBM diet than other groups (p<0.05) except 1.0 ESBM. In phase II (d 14 to 28), there was no significant differences in production traits among treatments. For overall period (d 0 to 28), piglets fed diets with high sodium sulfite grew faster than piglets fed control and 0.5 ESBM diets. The highest ADG and the best FeR were obtained in piglets fed diets with 1.5 ESBM during the entire period. Piglets fed 1.5 ESBM diet showed significantly higher crude protein digestibility than 0.5 ESBM (p<0.05) at d 14 post-weanling, but not at d 28 post-weanling. There were no significant differences in digestibilities of total amino acids. In conclusion, the addition level of 1~1.5% sodium sulfite for SBM extrusion could be favorable for rate and efficiency of growth in weaning pigs.

Subjective Wear Test and Fit of Women's Sports Underwear Made of Cool-Touch Fabric (냉감소재로 제작한 여성 스포츠 언더웨어의 피트성과 착용시 주관적 평가)

  • Kim, Soyoung;Lee, Heeran;Choi, Jiyoung;Hong, Kyunghi
    • The Korean Journal of Community Living Science
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    • v.28 no.4
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    • pp.505-514
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    • 2017
  • Although studies on the development of cool touch fabrics have been conducted widely, the effects of fitted pattern on enhancing the cooling sensation are insufficient. To investigate the effect of cool-touch fabric and fit of women's sports underwear, 3D and 2D patterns of sleeveless top and sports leggings were constructed. The performance of cool touch was tested by the Qmax value and wear test with nine subjects. Objective fit evaluation was observed by 3D virtual clothing using Clo software. Subjects rated wearing sensation such as 'cooling sensation, fit, wear comfort and preferences of purchase' using Likert's scale in the environmental chamber at $25^{\circ}C$, 45 %RH. The Qmax value of the cool touch fabric was higher than that of the PET fabric, which was well reflected in 'cooling sensation', especially in the case of a tight-fitted 3D pattern. The cooling sensation of the cool-touch fabric was not significantly elevated with 3D tight pattern as long as the size of the 2D pattern was similar to that of 3D pattern. However, the purchase preference was highly correlated with 3D fit and wear comfort.

NOTE ON UPPER BOUND SIGNED 2-INDEPENDENCE IN DIGRAPHS

  • Kim, Hye Kyung
    • East Asian mathematical journal
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    • v.28 no.5
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    • pp.579-585
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    • 2012
  • Let D be a finite digraph with the vertex set V(D) and arc set A(D). A two-valued function $f:V(D){\rightarrow}\{-1,\;1\}$ defined on the vertices of a digraph D is called a signed 2-independence function if $f(N^-[v]){\leq}1$ for every $v$ in D. The weight of a signed 2-independence function is $f(V(D))=\sum\limits_{v{\in}V(D)}\;f(v)$. The maximum weight of a signed 2-independence function of D is the signed 2-independence number ${\alpha}_s{^2}(D)$ of D. Recently, Volkmann [3] began to investigate this parameter in digraphs and presented some upper bounds on ${\alpha}_{s}^{2}(D)$ for general digraph D. In this paper, we improve upper bounds on ${\alpha}_s{^2}(D)$ given by Volkmann [3].