• Journal of Embryo Transfer
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• v.20 no.1
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• pp.25-33
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• 2005

This study is a part of research that development of effective genetic resources preservation system using the in vitro spermatogenesis, in vitro insemination and culture system. We aimed for establishment of in vitro culture system with in vitro activated porcine oocytes. The porcine oocytes were matured for 48 hours in $TCM199+10\%$ FCS and activated with $7\%$ ethanol. The activated oocytes were cultured for 7 days in $TCM199+10\%$ FCS or $NCSU23+0.4\%$ BSA medium. The activated oocytes were not developed to the blastocyst stage in $TCM199+10\%$ FCS medium. However in $NCSU23+0.4\%$ medium, those were developed to blastocyst with $3\%$ of treated oocytes. We extended maturation duration of porcine follicular oocytes fur 48, 52, 56, 60, 64, 68, and 72 hours and activated with $7\%$ ethanol and cultured using $NCSU23+0.4\%$ BSA medium. The six percents of activated oocytes were developed to blastocyst in 48 hours and $10\%$ in 52 hours with comparatively low rates suggested to be not fully activated by regenerated MPF. Maturation durations from 56 hours to 68 hours supported to develop upto $11.9\~18.3\%$ of blastocysts. However the developmental rate was declined to $7.2\%$ at 72 hours of maturation duration because of cytoplasmic deterioration. The assumed time window for activation will be $56\~68$ hours of maturation duration. When the matured oocytes were activated with electric pulse of 1, 1.2, 1.4, 1.6, 1.8 and 2.0kV/cm for $80{\mu}s$, although appling the electric current once was not enough for activation, appling twice with 1.6kV/cm for $80{\mu}s$ was shown the highest developmental rate with $11.3\%$. When those were compared with activating methods, $15.7%$ of blastocyst rate was obtained in the $7\%$ ethanol. That was higher than those in electric pulse with $9.5\%$ and calcium ionophore method with $5.8\%$. In this experimental condition, the $7\%$ ethanol treatment was the most effective method for activating porcine oocytes.

• Korean Journal of Weed Science
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• v.2 no.1
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• pp.7-12
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• 1982

The experiment was studied to know the competitive ability of rice against weeds as affected by rice maturity and plant spacing. For both cultivars, Tongil (12 days later) and Yeongnamjosaeng, the least amount of weed was obtained from the 10 ${\times}$ 10cm plant spacing while no difference between 40 ${\times}$ (10 ${\times}$ 10cm) and 30 ${\times}$ 15cm plant spacings was observed. When weed was nor pulled out by hand, significant yield loss was resulted in 30 ${\times}$ 15cm and 40 ${\times}$ (10 ${\times}$ 10cm) plant spacings while no difference between weeding regime was observed at the 10 ${\times}$ 10cm plant spacing in both cultivars. Rice grain yield was unaffected by plant spacing for hand weeded plot in both rice cultivars. For noweeding plot, however, the highest grain yield was obtained from 10 ${\times}$10cm plant spacing and also this yield was not significantly different from the yield of hand weeded plot while significant yield loss was recognized at the 30 ${\times}$ 15cm and 40 ${\times}$ (10 ${\times}$ 10cm) plant spacings in noweeding plot. There was also a good relationship between weed weight and grain yield for both cultivars (Tongil; Y = 5.68 - 0.0127 X, r = -0.814$^*$; Yeongnamjosaengv Y = 5.04 - 0.0314 X, r =-0.9704$^{**}$). Based on these relationship, weed weight of 188.2g/sq.m for Yeongnamjosaeng and 223.6g/sq.m for Tongil was needed to reduce rice yield by 50%, respectively. This result implied that Tongil compete more efficiently against weed than Yeongnamjosaeng and hence, could be concluded that late maturing cultivar was more competitive against weed compared to early maturing cultivar.

• The Journal of the Petrological Society of Korea
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• v.9 no.2
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• pp.84-98
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• 2000

Daejeon-sa basalt in the Mt. Juwang area composed of 12 basalt flows alternate with 9 peperites and each basalt and peperite has the variety of thickness. Peperites yielded in Daejeon-sa basalt are mixed of basalt with reddish shale, of which textural type is globular peperite. Basalts yielded in Daejeon-sa basalt are massive basalt without vesicule, although sometimes vesicules are founded in upper within a flow unit. The basalt has mainly pseudomorph of olivine as phenocryst, and also plagioclase and clinopyroxene phenocryst. Matrix is mainly subophitic texture. The plotting result on the TAS diagram shows these basalts belong to the sub-alkaline, and it can be subdivided into calc-alkaline series on the basis of the diagram of Si02 vs. K20 and of alkali index vs. A1203 diagram. According to plots of wt.% oxides vs. wt.% MgO, abundances of A1203 and CaO increase with decreasing MgO while F ~ dOecre~ase . With decreasing MgO compatible elements decrease while incompatible elements increase. In spider diagram of MORB-normalized trace element patterns, HFS elements are nearly similiar with MORB, but LIL elements are enriched. Especially, contents of Ce, F: and Sm are enriched but Nb is depleted. In the chondrite-normalized REE patterns light REEs are enriched than heavy REEs. Tectomagmatic discrimination diagrams shows basalts in the study area are formed in the tectonomagmatic environment of subduction zone under continental margin. This result accord with characters of chemical composition mentioned above. Cr vs. Y diagram and CeM, vs. Ce diagram show that the primary magma of the basalts may formed by the about 15% partial melting of garnet-peridotite in the mantle wedge. After then, Daejeon-sa basalts may formed from evolved magma undergone mainly olivine fractional crystallization and contarnination of crustal materials before eruption.

• Journal of Preventive Medicine and Public Health
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• v.31 no.1 s.60
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• pp.91-103
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• 1998

Under experimental conditions, UVB radiation, a type of ultra violet radiation, has shown to .elate with the occurrence of skin erythema (sun-burn) in human and skin cancer in experimental animal. Cumulative exposure to UVB is also believed to be at least partly responsible for the 'aging' process of the skin in human. It has also been observed to have an effect of altering DNA (deoxyribonucleic acid). UVB radiation is both an initiator and a promoter of non-melanoma skin cancer. Meta-analysis is a new discipline that critically reviews and statistically combines the results of previous researches. A recent review of meta-analysis in the field of public health emphasized its growing importance. Using a meta-analysis in this study, we explored more reliable dose-response relationships between UVB radiation and skin cancer incidence. We estimated skin cancer incidence using measured UVB radiation dose at a local area of Seoul (Shin chou-dong). The studies showing the dose-response relationships between UVB radiation and non-melanoma skin cancer incidence were searched and selected for a meta-analysis. The data for 7 reported epidemiological studies of three counties (USA, England, Australia) were pooled to estimated the risk. We estimated rate of incidence change of skin cancer using pooled data by meta-analysis method, and exponential and power models. Using either model, the regression coefficients for UVB did not differ significantly by gender and age. In each analysis of variance, non-melanoma skin cancer incidence after removing the gender and age and UVB effects was significant (p>0.01). The coefficients for UVB dose were estimated $2.07\times10^{-6}$ by the exponential model and 2.49 by the power model. At a local area of Seoul (Shinchon-Dong), BAF value were estimated 1.90 and 2.51 by the exponential and power model, respectively. The estimated BAP value were increased statistical power than that of primary studies that using a meta-analysis method.

• Journal of Dairy Science and Biotechnology
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• v.27 no.2
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• pp.7-16
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• 2009

Appenzeller cheese samples were prepared by addition of 0.5, 1.0, and 2.0% green tea (Camellia sinensis, CS) powder and control cheese. We examined various quality characteristics of the novel cheese, such as viable-cell counts, pH, water-soluble nitrogen (WSN), non-casein nitrogen (NCN), non-protein nitrogen (NPN), and catechin level during maturation for 16 weeks at $14^{\circ}C$. To develop a Korean natural cheese containing green tea powder, we also analyzed the changes in the polyacrylamide gel electrophoresis pattern, chemical composition, and sensory qualities. The viable cell counts of the samples were not significantly different. Until the $3^{rd}$ week, the pH of the CS cheese decreased with an increase in the maturation time. However, the pH gradually increased by the $12^{th}$ week, while WSN, NCN, NPN also increased. The WSN, NCN, NPN, and catechin values for the CS cheese samples were significantly higher than the values for the control cheese. The polyacrylamide gel electrophoretic pattern of caseins for the CS cheese indicated that this cheese degraded more rapidly than the control cheese did. In the sensory evaluation, cheese with 1.0% CS powder showed the highest scores in taste and appearance and good scores in flavor and texture. These results indicate that 1.0% CS is the optimal value for addition to cheese, and cheese containing 1.0% CS shows good physiological properties and reasonably high overall sensory acceptability.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.1
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• pp.49-60
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• 2008

A study was conducted to determine the effect of alcohol-fermented feedstuff formulated with byproducts on the fermentation characteristics and dry matter disappearance in the rumen. Dietary treatments were either a soybean curd-based alcohol-fermented feedstuff(AFS) and brewery grain-based alcohol-fermented feedstuff(AFB). The AFS and AFB are composed of 50% commercial beef cattle feed, 50% soybean curd dreg, 5% molasses and 0.5% yeast, and 25% commercial beef cattle feed, 25% brewery grain, 25% soybean curd dreg, 25% corn grit, 5% molasses and 0.5% yeast, respectively. The ruminally cannualted Korean cattle were utilized to investigate the change of ammonia, pH alcohol, volatile fatty acids, and DM digestibility at 0, 2, 4, 8 and 12 hr after feeding. The rumen ammonia concentrations were significantly lower in AFS and AFB with incubation time, especially at 6 hr incubation(AFS, 0.7 mg/dl; AFB, 1.5 mg/dl; control 2.5 mg/dl). Lower rumen pH was observed in AFS and AFB during the early stage of incubation, but no significant difference was found at late stage of incubation. The total VFA concentrations were not affected by diet treatments at 2 hr incubation time, but the concentration significantly decreased after that. The dry matter disappearance was significantly lower in AFS and AFB during the early stage of incubation. However, the dry matter disappearance of AFS and AFB was similar to that of control during the late stage of incubation. It is concluded that the industrial byproducts such as soybean curd dreg and brewery grain were effective materials to make an alcohol fermented feedstuffs and resulted in better fermentation characteristics in the rumen when both were applied to Hanwoo.

• Korean Journal of Agricultural Science
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• v.14 no.2
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• pp.358-372
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• 1987

This study was performed to obtain data which can be applied to use of water-proof mortars. The data was based on the properties of water-proof mortars depending upon various mixing ratios to compare those of cement mortar. The water-proof agents used were retard and accelerate type which are being used as mortar structures. The water-proof agents, mixing ratios of cement to fine aggregate were 1:1, 1:2, 1:3 and 1:4. The results obtained were summarised as follows; 1. The results of flow test, water-cement ratio was increased with the increasing of mixing ratio. 2. The permeability were increased in poorer mixing ratio and higher water pressure. 3. The bulk density was decreased with the increasing of mixing ratio, and compressive and tensile strength were increased with increasing of the bulk density. 4. At 1:1 mixing ratio, the highest strengths were showed and strengths were decreased with the increasing of mixing ratio. 5. The absorption rates were increased in- poorer mixing ratio 6. The correlation between W/C, permeability, bulk density, compressive strength and absorption rate were highly significant as a straight line, respectively.

• Journal of Animal Environmental Science
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• v.15 no.3
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• pp.251-262
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• 2009

In this research dairy cattle barn facilities what are 4,198 houses hold over 50 heads were surveyed by scale and province. Full-time farms hold over 50 heads breed total 344,514 heads. Each of Farms holds 50 to 99 heads were 79.8 percent and breed average 82.1 heads. Dairy cattle barns were constructed september 1995 averagely. Each of barns have $1,740.0\;m^2$ scale. The construction type of dairy cattle barn was almost litter barn type 84.0%, freestyle type 5.1%, mooring+litter ground type 17.3% and other types 4.4%. The litter barn type was popular in small farms. But in large farms, freestyle type was popular than small farms. The construction type of dairy cattle barn was almost litter barn type 84.0%, freestyle type 5.1%, moohng+ltter ground type 17.3% and other types 4.4%. Type of dairy cattle robotic milking system was pipeline 41.5%, herringbone 22.8% and tandem 35.8%. The pipeline type was popular in small farms which have 50~99 heads. But in large farms which have over 200 heads, tandem type was popular than small farms. Proportion of floor type of dairy cattle barn was almost litter type 94.9%. Scraper type was popular in large farms than in small farms. Proportion of roof type of dairy cattle barn was slate 32.5%, vinyl 16.3%, sunlight 11.1%, panel 10.9, zinc plate 8.8 and steel plate 8.3%. Roof type was lots of slate type before 1995. But vinyl type is increasing after 1995. Proportion of wall type of dairy cattle barn was almost open type 83.3% and winch-curtain 26.8%. Utilization period of dairy cattle barn was 9.2 years about milker, 7.9 years about automatic feeder, 9.2 years about waterer and 10.4 years about electric facilities. In this results, there were lots of improvements about automatic feeder.

• Korean Journal of Cognitive Science
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• v.13 no.3
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• pp.1-10
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• 2002

This study was performed to analyze the physical attributes of the faces and affective words on the fares. For analyzing physical attributes inside of a face, 36 facial features were selected and almost of them were the lengths or distance values. For analyzing facial contour 14 points were selected and the lengths from nose-end to them were measured. The values of these features except ratio values normalized by facial vortical length or facial horizontal length because the face size of each person is different. The principal component analysis (PCA) was performed and four major factors were extracted: 'facial contour' component, 'vortical length of eye' component, 'facial width' component, 'eyebrow region' component. We supposed the five-dimensional imaginary space of faces using factor scores of PCA, and selected representative faces evenly in this space. On the other hand, the affective words on faces were collected from magazines and through surveys. The factor analysis and multidimensional scaling method were performed and two orthogonal dimensions for the affections on faces were suggested: babyish-mature and sharp-soft.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.4
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• pp.503-526
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• 1996

A synoptic survery of chemical characteristics in the last Sea of Korea was carried out at the 11 stations near Ullungdo in November, 1994 on board R/V Tam-Yang. On the basis of the vortical distribution patterns of temperature, salinity and dissolved oxygen, water masses in the study area are divided into five groups; 1) Tsushima Surface Water (TSW), 2) Tsushima Middle Water (TMW), 3) East Sea Intermediate Water (ESIW), 4) last Sea Proper Water (ESPW), 5) Mixed Water (MW). In the vertical profiles of nutrients, the concentrations were very low in the surface layer and increased rapidly near the thermocline. There was a slight decrease in the ESIW and the concentrations were constant with the depth below 300m except dissolved silicate which still increased with depth. Relatively high value of Si/P ratio (25.2) in ESPW, whick is the oldest water mass, suggests that Si is regenerating more slowly compared to other nutrients. The relatively high value of N/P ratio (18.6) in the surface layer might be related to high vertical eddy diffusivity $(K_z)$ of $1.19\;cm^{2}/sec$ and high nitrate upward flux of $103.7\;{\mu}g-at/m^{2}/hr$, compared to the values reported in other areas. Apparent Oxygen Utilization (AOU) was very low in the surface layer and increased in the TMW, but there was a slight decrease in the ESIW. The highest value of AOU occurred in the ESPW. The slpoe of P/AOU was 0.50. The study on the relationship between water masses and nutrient distribution patterns is important in understanding the regeneration processes of nutrients in the polar region of the last Sea.