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Intraspecies Volatile Interactions Affect Growth Rates and Exometabolomes in Aspergillus oryzae KCCM 60345

  • Singh, Digar;Lee, Choong Hwan
    • Journal of Microbiology and Biotechnology
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    • v.28 no.2
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    • pp.199-209
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    • 2018
  • Volatile organic compounds (VOCs) are increasingly been recognized as the chemical mediators of mold interactions, shaping their community dynamics, growth, and metabolism. Herein, we selectively examined the time-correlated (0 D-11 D, where D = incubation days) effects of intraspecies VOC-mediated interactions (VMI) on Aspergillus oryzae KCCM 60345 (S1), following co-cultivation with partner strain A. oryzae KACC 44967 (S2), in a specially designed twin plate assembly. The comparative evaluation of $S1_{VMI}$ (S1 subjected to VMI with S2) and its control ($S1_{Con}$) showed a notable disparity in their radial growth ($S1_{VMI}$ < $S1_{Con}$) at 5 D, protease activity ($S1_{VMI}$ > $S1_{Con}$) at 3-5 D, amylase activity ($S1_{VMI}$ < $S1_{Con}$) at 3-5 D, and antioxidant levels ($S1_{VMI}$ > $S1_{Con}$) at 3 D. Furthermore, we observed a distinct clustering pattern for gas chromatography-time of flight-mass spectrometry datasets from 5 D extracts of $S1_{VMI}$ and $S1_{Con}$ in principle component analysis (PC1: 30.85%; PC2: 10.31%) and partial least squares discriminant analysis (PLS-DA) (PLS1: 30.77; PLS2: 10.15%). Overall, 43 significantly discriminant metabolites were determined for engendering the metabolic variance based on the PLS-DA model (VIP > 0.7, p < 0.05). In general, a marked disparity in the relative abundance of amino acids ($S1_{VMI}$ > $S1_{Con}$) at 5 D, organic acids ($S1_{VMI}$ > $S1_{Con}$) at 5 D, and kojic acid ($S1_{VMI}$ < $S1_{Con}$) at 5-7 D were observed. Examining the headspace VOCs shared between S1 and S2 in the twin plate for 5 D incubated samples, we observed the relatively higher abundance of C-8 VOCs (1-octen-3-ol, (5Z)-octa-1,5-dien-3-ol, 3-octanone, 1-octen-3-ol acetate) having known semiochemical functions. The present study potentially illuminates the effects of VMI on commercially important A. oryzae's growth and biochemical phenotypes with subtle details of altered metabolomes.

Stability of Spheroplasts from Saccharomyces cerevisiae D-71 and Zygosaccharomyces rouxii SR-S (Saccharomyces cerevisiae D-71과 Zygosaccharomyces rouxii SR-S로부터 조제한 원형질체의 안정성)

  • 정창기;김찬조;이종수
    • Microbiology and Biotechnology Letters
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    • v.16 no.4
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    • pp.293-296
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    • 1988
  • Stability of spheroplasts prepared from Saccharomyces cerevisiae D-71, a thermophilic strain and Zygosaccharomyces rouxii SR-S, an osmotolerant strain were studied. Stability of spheroplast from Saccharomyces cerevisiae D-71 was highest in 0.8M KCI and 1.0M sorbitol ; that from Zygosaccharomyces rouxii SR-S was highest in 0.4M KCI and mannitol and that from both strains was less than 10% for sonic oscillation at 20Kc for 60 sec. In centrifugation at 10000 x g for 10 min., stability of spheroplast from Saccharomyces cerevisiae D-71 was 93% and that from Zygosaccharomyces rouxii SR-S was 84%. Breakage of spheroplast from Saccharomyces cerevisiae D-71 was 99% and that from Zygosaccharomyces rouxii SR-S was 55% for UV irradiation with 15W UV lamp at a distance of 20 cm for 60 min.

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6-18 GHz MMIC Drive and Power Amplifiers

  • Kim, Hong-Teuk;Jeon, Moon-Suk;Chung, Ki-Woong;Youngwoo Kwon
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.2 no.2
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    • pp.125-131
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    • 2002
  • This paper presents MMIC drive and power amplifiers covering 6-18 ㎓. For simple wideband impedance matching and less sensitivity to fabrication variation, modified distributed topologies are employed in the both amplifiers. Cascade amplifiers with a self-biasing circuit through feedback resistors are used as unit gain blocks in the drive amplifier, resulting in high gain, high stability, and compact chip size. Self impedance matching and high-pass, low-pass impedance matching networks are used in the power amplifier. In measured results, the drive amplifier showed good return losses ($S_11,{\;}S_{22}{\;}<{\;}-10.5{\;}dB$), gain flatness ($S_{21}={\;}16{\;}{\pm}0.6{\;}dB$), and $P_{1dB}{\;}>{\;}22{\;}dBm$ over 6-18 GHz. The power amplifier showed $P_{1dB}{\;}>{\;}28.8{\;}dBm$ and $P_{sat}{\;}{\approx}{\;}30.0{\;}dBm$ with good small signal characteristics ($S_{11}<-10{\;}dB,{\;}S_{22}{\;}<{\;}-6{\;}dB,{\;}and{\;}S_{21}={\;}18.5{\;}{\pm}{\;}1.25{\;}dB$) over 6-18 GHz.

Physiological Response of Four Corn Cultivars to Soil Salinity (토양염농도에 따른 식용옥수수 품종들의 생장특성)

  • Kim, Sun;Choi, Weon-Young;Jeong, Jae-Hyeok;Lee, Kyung-Bo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.3
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    • pp.293-298
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    • 2014
  • This study conducted experiments on the reclaimed land of Saemangeum located in Jeongrabuk-do in order to gain basic information about growth characteristics and yield ability according to soil salinity. Having soil excluding salt as a control group, this study adjusted the specimens' soil salinity to level 4 and then planted four varieties including Ilmichal Corn to investigate the growth or grain yield according to salinity. About the corn establishment rate according to soil salinity, over 97% up to $3.2dS\;m^{-1}$ was established, and then, it was reduced gradually according to the increase of concentration. According to the salt concentration of soil more required growth duration from seeding to heading comparing to non-treatment salt was delayed, at $1.6dS\;m^{-1}$, two days were delayed, at $3.2dS\;m^{-1}$, four to six days were delayed differently by varieties, and at $4.8dS\;m^{-1}$, six to 10 days were delayed. About the plant fresh weight according to soil salinity, Chalok 4 and Eolrukchal indicated 93%~97% or so compared with the salt-free one at $1.6dS\;m^{-1}$, and Chalok No. 4 showed 79% at the salinity of $3.2dS\;m^{-1}$, too, and it was a relatively higher growth percentage than those of the other varieties. In terms of dried seed weight according to soil salinity, compared with the corns cultivated in the control group, averagely 12.1% was lowered at the time of cultivation at $1.6dS\;m^{-1}$, and $3.2dS\;m^{-1}$ 40% was lowered, and about 70% was lowered at $4.8dS\;m^{-1}$. According to the result of examining the point of time that dried seed start to reduce due to soil salinity with the regression equation, soil salinity which starts the reduction of grain weight is $1.67dS\;m^{-1}{\sim}2.18dS\;m^{-1}$, and it differs by varieties, and EC of 50% that the yield reduces in half is $2.96dS\;m^{-1}{\sim}4.45dS\;m^{-1}$. And the degree of influence on each of the growth factors according to soil salinity is founded to be in the order of establishment rate

LUMINOSITY PROFILES OF dE AND dS0 GALAXIES IN THE VIRGO CLUSTER

  • Kim, Kyoo-Hyun;Lee, Kyung-Hoon;Ann, Hong-Bae
    • Journal of The Korean Astronomical Society
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    • v.39 no.3
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    • pp.57-71
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    • 2006
  • We investigated the structural parameters of a sample of 30 dwarf galaxies(15 dEs and 15 dS0s) in the Virgo Cluster using i-band images from the Sloan Digital Sky Survey Data Release 4. Among 28 galaxies for which surface brightness profiles were derived from ellipse fittings, 23 galaxies had a single component that was adequately described by a generalized $S\acute{e}rsic$ function with a shape parameter ranging from n=0.5 to 2, while 5 galaxies(2 dEs and 3 dS0s) had bulge and disk components that were fitted by a generalized $S\acute{e}rsic$ function and an exponential function, respectively. Since the majority of dwarf galaxies in the present sample had a single component, it seems likely that genuine dS0 galaxies that have disk and bulge components are quite rare in the Virgo Cluster. The similarity in structural parameters of genuine dS0 galaxies in the Virgo Cluster with those of Magellanic-type galaxies implies that the progenitors of dwarf lenticular galaxies in the Virgo Cluster were most likely Magellanic-type galaxies if dS0s are harassed late-type spirals.

A study on wideband strip-line balun using a power divider and a phase converter (전력 분배기와 위상 변환기를 이용한 광대역 스트립라인 발룬에 관한 연구)

  • Lee, Chang-Seok;Park, Ung-Hee
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.12 no.7
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    • pp.1160-1164
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    • 2008
  • This paper presents an wideband stripline balun. And, the proposed balun is composed of one power divider and two phase converters on stripline substrate. The target characteristics of the proposed balun is the combination loss of below -0.05 dB, an amplitude imbalance of ${\pm}0.5\;dB$ and a phase imbalance of $180{\pm}10$ degrees, with the reflection coefficient(S11) of below -10 dB over frequencies ranging from 500 to 1500 MHz. The fabricated balun occupies the area of $12(W){\times}220(L){\times}2.3(T)\;mm3$. Experimental measurement shows that the fabricated balun has an amplitude imbalance of ${\pm}0.7\;dB$, a phase imbalance of $180{\pm}8$ degrees and an insertion loss of about -2 dB with the reflection coefficient(S11) of below -10 dB over frequencies ranging from 500 to 1,500 MHz.

Changes of Soil Salinity due to Flooding in Newly Reclaimed Saline Soil (신간척지 토양에서 담수에 의한 토양염도 변화에 대한 개관)

  • Ryu, J.H.;Yang, C.H.;Kim, T.K.;Lee, S.B.;Kim, S.;Baek, N.H.;Choi, W.Y.;Kim, S.J.;Chung, D.Y.
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.spc
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    • pp.45-46
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    • 2009
  • This study was carried out to identify the changes of EC during desalinization due to flooding in newly reclaimed saline soil. To do this, experimental plots were made of rotary tillage+water exchanging plot, flooding plot and rainfall flooding plot. In rotary tillage+water exchanging plot, drainage, rotary tillage and flooding were conducted at the interval of 7 days. In rotary tillage+water exchanging plot and flooding plot, plots were irrigated at the height of 10 cm. After 38 days desalinization, changes of EC values at top soil (0~20 cm) were as follows. In rotary tillage+water exchanging plot, EC decreased from $21.38dS\;m^{-1}$ to $2.16dS\;m^{-1}$ and in flooding plot, EC decreased from $13.97dS\;m^{-1}$ to $2.22dS\;m^{-1}$. In rotary tillage+water exchanging plot and flooding plot, EC values decreased below the EC criterion ($4.0dS\;m^{-1}$) of saline soil. In rainfall flooding plot, EC values decreased or increased according to amounts of rainfall and rainfall time. After 38 days, EC decreased from $16.7dS\;m^{-1}$ to $12.35dS\;m^{-1}$. In flooding plot, changes of EC due to soil depth were investigated. After 38 days desalinization, changes of EC due to soil depth were as follows. At 0~10 cm depth, EC value decreased from $13.08dS\;m^{-1}$ to $0.74dS\;m^{-1}$ (94.3% of salt was desalinized). At 10~20 cm depth, EC value decreased from $14.80dS\;m^{-1}$ to $3.69dS\;m^{-1}$ (75.2% of salt was desalinized). At 20~30 cm depth, soil was desalinized slowly compared with upper soil, EC value decreased from $13.57dS\;m^{-1}$ to $6.93dS\;m^{-1}$ (48.9% of salt was desalinized).

Culture study on the hybrid by interspecific crossing between Porphyra pseudolinearis and P. dentata (Bangiales, Rhodophyta), two dioecious species in culture

  • Kim, Nam-Gil
    • ALGAE
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    • v.26 no.1
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    • pp.79-86
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    • 2011
  • Physiological studies on the hybrid by crossing between two dioecious species, Porphyra pseudolinearis and P. dentata from Korea were conducted at constant temperatures (5, 10, 15, 20, and $25^{\circ}C$), at photon flux densities (10, 20, 40, and $80\;{\mu}mol\;m^{-2}s^{-1}$) under photoperiods (14 L : 10 D and 10 L : 14 D). In the hybrid, higher growth of conchocelis was observed at 20 and $40\;{\mu}mol\;m^{-2}s^{-1}$ under 14 L : 10 D. Conchosporangial branches were produced under $10-80\;{\mu}mol\;m^{-2}s^{-1}$ at only $25^{\circ}C$, and were abundant when the conchocelis was cultured under 10 L : 14 D. Foliose thalli of the hybrid grew well at the conditions of $10-20^{\circ}C$, 10 L : 14 D and $15-20^{\circ}C$, 14 L : 10 D. The foliose thalli grew very slowly at $5^{\circ}C$ and $30^{\circ}C$, respectively. No archeospores were observed at any culture conditions. Spermatangial and zygotosporangial sori were formed at the marginal portion of mature thallus. Zygotospores from the hybrid were released at $10-2^{\circ}C$ under both photoperiods, and gave rise to form conchocelis filament. Monoecious thalli were observed at $10^{\circ}C$ under 14 L : 10 D. Neither monospores nor protothalli were produced from the conchocelis in culture.

Acoustic Properties of Electrostrictive Buzzer Using PZN-BT-PT Ceramics (PZN-BT-PT 세라믹스를 이용한 전왜 BUZZER의 음향 특성)

  • 유준현;김현재;박창엽
    • The Transactions of the Korean Institute of Electrical Engineers
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    • v.41 no.1
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    • pp.50-55
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    • 1992
  • Piezoelectric Buzzer which has been generally used requires about 30kV/cm poling process and has the aging effects. In this study, 0.85 PZN - 0.10 BT - 0.05 PT system ceramics with additives of 0 - 2 wt% YS12TOS13T were fabricated and investigated on electromechanical coupling coefficient(kS1pT), electric field induced charge coefficient(dS131T), and sound level. As the results, in the 0.4 wt% YS12TOS13T added composition ceramics compared with the basic, kS1pT was increased from 0.355 to 0.39 and induced piezoelectric d constant increased from 204 to 220 x 10S0-12T (C/N) and sound level of electrostrictive Buzzer has the highest value of 71.5 dB under 8kV/cm bias electric field.

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A successful Mustard operation for complete transposition of the great arteries combined with VSD, ASD, dextrocardia and PS: a report of one case (심실중격결손, 심방중격결손, 폐동맥협착과 우심증을 동반한 완전대혈관전위증의 치험)

  • 조중구
    • Journal of Chest Surgery
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    • v.15 no.3
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    • pp.346-354
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    • 1982
  • A Complete transposition of the great arteries combined with V.S.D, A.S.D, dextrocardia, and P.S is a rare congenital anomaly. The patient was a 10 year-old female whose complaints were frequent URI, exertional dyspnea, and cyanosis at rest since birth. Cheat X-ray films showed Dextrocardia ; situs inversus, moderate cardiomegaly, and Characteristic egg-shape heart shadow. E.K.G, Echocardiography, Cardiac Catheterization, and Angio-Cardiography were performed. Open heart Surgery was done under diagnosis of d-TGA, Dextrocardia, V.S.D, A.S.D, and P.S. At the time of Operation, Dextroeardia, T.G.A, Secndum type A.S.D, A.S.D, and P.S. At the time of Operation, Dextroeardia, T.G.A, Secndum type A.S.D. ($2.0{\times}2.0cm$. in diameter), V.S.D. type II ($1.5cm{\times}1.5cm$ in diameter), and pulmonary valvular stenosis were noted. Mustard operation using pericardial Baffle in the atrium for T.G.A. was perforsned. Teflon patch graft for closure of V.S.D. through tricuspid orifice and pulmonary Valvulotomy through pulmonary arteriotomy were performed. The post-op, low cardiac output Syndrome and dysrhythmicawere developed till Postoperative day #7, so that was controlled by inotropic and antiarrhythmic agents. After that, patient's conditions were Uneventful.

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