• Title/Summary/Keyword: D1-D2 region

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Cercarial Chaetotaxy of Clonoychis sinensis (간흡충 세르카리아의 Chaetotaxy에 관한 연구)

  • 민득영;김재진
    • Parasites, Hosts and Diseases
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    • v.26 no.2
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    • pp.113-116
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    • 1988
  • The pattern of sensory papillae, chaetotaxy, of the cercaria of Clenorchis sinensis was observed, The chaetotaxy was as follows; 5~6 Ci 1, 4~5 Ci 2, 5, ~6 Ci3 at 1st row, 4 Cii 1, 2 Cii2, 4 Cii3, 5~6 Cii4 at 2nd row, 3~4 Ciii 1, 2~3 Ciii 2 at 3rd row, and 2 Civl, 2~3civ2, 2~3 Civ 3, at 4th row, in cephalic region; 2 AiV, 1 AiD, 2 AiiV, 1 AiiD, 2 Aiiiv, 2 AiiiD, 1 AivV, 1 AivD, 1 PiiD, 1 PiiiD, in ventral(V) and dorsal(D) portions of body. Caudal region revealed 2-2-2-2 formula.

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Blastobotrys illinoisensis, an Unrecorded Anamorphic Yeast Strain Isolated from the Gut of the Earthworm Eisenia fetida

  • Ji Yun Son;Myung Kyum Kim
    • The Korean Journal of Mycology
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    • v.50 no.4
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    • pp.373-381
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    • 2022
  • Strain E4, an unrecorded species of dimorphic fungi, was isolated from the gut of earthworms collected in Gyeonggi Province, South Korea. Nucleotide sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region revealed that this species is a member of the genus Blastobotrys, Blastobotrys illinoisensis. Strain E4 differed from its closest known species, B. mokoenaii and B. malaysiensis, by harboring 3-5 and 12-14 nucleotide substitutions in the D1/D2 and ITS regions, respectively. Phylogenetic analysis based on concatenated sequences of the D1/D2 region of the LSU rRNA gene and the ITS region also indicated that strain E4 belongs to the Blastobotrys clade and is distinct from other related species in the clade. The previously unreported isolate could be distinguished from closely related species by its inability to ferment carbon sources. To our knowledge, this is the first report on the isolation of Blastobotrys species from the gut of earthworms in Korea. The strain used was E4 (=KCTC 27831=JCM 33428).

Study on Sensory Papillae of Metagonimus yokogawai Cercaria (Metagonimus yokogawai 세르카리아의 감각유두에 관한 연구)

  • 김재진;민득영소진탁
    • Parasites, Hosts and Diseases
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    • v.22 no.1
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    • pp.11-20
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    • 1984
  • A number of studies on the papillae of cercariae of trematodes reported that the papillar patterns (or chaetotaxy) of cercariae might be an excellent method to attain better understanding of the digenetic trematodes (Richard, 1971 ; Short and Cartrett, 1973; Bayssade-Dufour, 1979) . The present study was aimed to determine the number, distribution pattern and structure of the sensory papillae of Metagonimus yokogawai cercariae, and to elucidate the chaetotaxy of this digenetic trematode. M. yokogawai cercariae were pipetted from a vial in which infected snails (Semisulcospira libertina) had been kept for 3 hours. The snails were collected from an endemic area of M. yokogawai, Boseong river in west-southern part of Korea. Observations of papillae were based on light microscopy of those stained with silver nitrate, and on scanning electron microscopy The results are summarized as follows: 1, All papillae observed were uniciliated. 2. Cilia in anterior tip were shorter than the others in other portions. 3. The body papillae were arranged in essentially symmetrical patterns, Total number of the papillae was 126(63 pairs) in average; anterior tip 40(20 pairs), ventral 20(10 pairs), lateral 42(21 pairs), and caudal 8(4 pairs). 4. The chaetotany of M. yokogawai cercaria was: Ci cycle ($3+3C_{I}V,{\;}2+2C_{I}L,{\;}2+3C_{I}D),{\;}C_{II}{\;}cycle(2C_{II}V,{\;}1C_{II}L,{\;}2C_{II}D),{\;}C_{lll}{\;}cycle{\;}(1+lC_{III}V,{\;}1C_{IlI}L),{\;}C_{IV}{\;}cycle{\;}(1C_{IV}V,{\;}IC_{lV}L){\;}in{\;}cephalic{\;}region:{\;}A_I(1A_{IV}V,{\;}1+2A_{I}L,{\;}1A_{I}D),{\;}A_{II}(1A_{II}V,{\;}1+3A_{II}L,{\;}1A_{II}D),{\;}A_{III}(1A_{III}V,{\;}1+1A_{III}L,{\;}1A_{III}D){\;}and{\;}A_{IV}(1A_{IV}V,{\;}2A_{IV}L)$ in antacetabular region: $1M_{I}V{\;}and{\;}2M_{I}L$ in median: $1+1P_{I}L,{\;}1P_{II}L,{\;}1P_{II}D,{\;}1P_{III}L,{\;}1P_{IV}L{\;}and{\;}1P_{IV}D$ in postacetabular region: 2-2-2-2 in caudal region.

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Correlation between total air pollutant emissions and incidence of type 1 diabetes in the Russian Federation

  • Choi, Hoon Sung;Kim, Jin Taek;Seo, Ji-Young;Linkov, Faina;Shubnikov, Evgeniy;Lee, Hong Kyu
    • Clinical and Experimental Pediatrics
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    • v.64 no.10
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    • pp.525-530
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    • 2021
  • Background: Exposure to air pollution (gaseous pollutants and/or particulate matter) has been associated with the incidence, prevalence, and mortality of type 1 diabetes (T1D). Purpose: To examine the quantitative relationship between air pollutant emissions and the incidence of T1D. Methods: We examined the association between the incidence of T1D and type 2 diabetes (T2D) in 2017 as well as that of T1D in patients younger than 15 years in 2016 with "emissions of air-polluting substances from stationary and mobile sources by regions of the Russian Federation in 2016" as reported by the Federal Diabetes Register of Russia downloaded from the Russian government website (http://www.mnr.gov.ru/docs/gosudarstvennye_doklady/o_sostoyanii_i_ob_okhrane_okruzhayushchey_sredy_rossiyskoy_federatsii/). Results: The incidence of T1D across all ages in each region of the Russian Federation correlated with the total air pollutants emitted in the region each year (r=0.278, P=0.013). The incidence of T2D was also correlated with the amount of air pollutants (r=0.234, P=0.037) and the incidence of T1D (r=0.600, P<0.001) in each country. Similarly, the incidence of T1D in patients younger than 15 years correlated with the total air pollutants emitted each year in each region (r=0.300, P=0.011). Conclusion: The quantitative relationship between the total air pollutants emitted and the incidence of T1D and T2D in the Russian Federation suggests that air pollution contributes to the development of T1D and T2D.

Phylogenetic Analysis of Dendropanax morbifera Using Nuclear Ribosomal DNA Internal Transcribed Spacer (ITS) Region Sequences (Internal transcribed spacer (ITS) region의 염기서열 분석에 의한 보길도산 황칠나무의 분자 계통학적 연구)

  • Shin, Yong Kook
    • Journal of Life Science
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    • v.26 no.11
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    • pp.1341-1344
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    • 2016
  • Dendropanax morbifera is an endemic tree species of Korea, it is restricted to the southern parts of Korea. The internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA) for Dendropanax morbifera grown at Bogil-do, Korea was determined. We investigated the sequence-based phylogenetic relationships of plants related and clarified its taxonomical position. The determined sequences consisted of 689 residues. ITS1 was 222 bp long while ITS2 was 233 bp long. The 5.8S rDNA was 160 bp long. The ITS region sequences of the Dendropanax species included in this study were obtained from GenBank. Oreopanax polycephalus was used as the outgroup. A pairwise alignment was calculated using the Clustal X program. A phylogenetic tree was constructed by the neighbor-joining method using the Tree view program. Sequence similarities among species including D. morbifera Bogil-do isolate showed the range 92.6 to 99.7% in sequence-based phylogenetic analysis using total 615 base pairs of ITS1, 5.8S rDNA and ITS2. D. morbifera Bogil-do isolate exhibited the highest degree of relatedness to D. chevalieri, sharing 99.7% ITS region similarity. D. morbifera Bogil-do isolate also showed to D. trifidus, sharing 99.4% ITS region similarity.

A Fast Algorithm for Region-Oriented Texture Coding

  • Choi, Young-Gyu;Choi, Chong-Hwan;Cheong, Ha-Young
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.9 no.6
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    • pp.519-525
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    • 2016
  • This paper addresses the framework of object-oriented image coding, describing a new algorithm, based on monodimensional Legendre polynomials, for texture approximation. Through the use of 1D orthogonal basis functions, the computational complexity which usually makes prohibitive most of 2D region-oriented approaches is significantly reduced, while only a slight increment of distortion is introduced. In the aim of preserving the bidimensional intersample correlation of the texture information as much as possible, suitable pseudo-bidimensional basis functions have been used, yielding significant improvements with respect to the straightforward 1D approach. The algorithm has been experimented for coding still images as well as motion compensated sequences, showing interesting possibilities of application for very low bitrate video coding.

FoxD2-AS1 is a prognostic factor in glioma and promotes temozolomide resistance in a O6-methylguanine-DNA methyltransferase-dependent manner

  • Shangguan, Wenbing;Lv, Xuyang;Tian, Nan
    • The Korean Journal of Physiology and Pharmacology
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    • v.23 no.6
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    • pp.475-482
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    • 2019
  • Glioma is the most common brain tumor with a dismal prognosis. While temozolomide (TMZ) based chemotherapy significantly improves survival in glioma patients, resistance against this compound commonly leads to glioma treatment failure. Overexpression of long-noncoding RNA (LncRNA) FoxD2 adjacent opposite strand RNA 1 (FoxD2-AS1) was identified to promote glioma development, but the role in TMZ resistance remains unclear. In this paper, we found that FoxD2-AS1 was overexpressed in recurrent glioma, high FoxD2-AS1 expression was significantly correlated with poor patient outcome. Methylation of $O^6$-methylguanine-DNA methyltransferase (MGMT) is significantly less frequent in high FoxD2-AS1 expression patients. Knockdown of FoxD2-AS1 decreased the proliferation, metastatic ability of glioma cells and promote the sensitivity to TMZ in glioma cells. Furthermore, knockdown of FoxD2-AS1 induced hypermethylation of the promoter region of MGMT. Our data suggested that FoxD2-AS1 is a clinical relevance LncRNA and mediates TMZ resistance by regulating the methylation status of the MGMT promoter region.

A Comparison of the Ability of Fungal Internal Transcribed Spacers and D1/D2 Domain Regions to Accurately Identify Candida glabrata Clinical Isolates Using Sequence Analysis

  • Kang, Min-Ji;Choi, Yoon-Sung;Kim, Sunghyun
    • Biomedical Science Letters
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    • v.24 no.4
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    • pp.430-434
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    • 2018
  • Candida glabrata is the second most prevalent causative agent for candidiasis following C. albicans. The opportunistic yeast, C. glabrata, is able to cause the critical bloodstream infections in hospitalized patients. Conventional identification methods for yeasts are often time consuming and labor intensive. Therefore, recent studies on sequence-based identification have been conducted. Recently, sequencing the D1/D2 domain of the large subunit ribosomal RNA gene and the internal transcribed spacers (ITS) 1 and ITS2 regions of the ribosomal DNA has proven useful for DNA-based identification of most species of fungi. In the present study, therefore, fungal ITS and D1/D2 domain regions were targeted and analyzed by DNA sequencing for the accurate identification of C. glabrata clinical isolates. A total of 102 C. glabrata clinical isolates from various clinical samples including bloodstream, catheterized urine, bile and other body fluids were used in the study. The results of the DNA sequence analysis showed that the mean standard deviation of species identity percent score between ITS and D1/D2 domain regions was $97.8%{\pm}2.9$ and $99.7%{\pm}0.46$, respectively. These results revealed that the D1/D2 domain region might be a better target for identifying C. glabrata clinical isolates based on DNA sequences than the ITS1 and ITS2 regions. However, in order to evaluate the usefulness of D1/D2 domain region for species identification of all Candida species, other Candida species such as C. albicans, C. tropicalis, C. dubliniensis, and C. krusei should be verified in further studies additionally.

Periodic Variations Of Water Temperature In The Seas Around Korea(II). Annual And Long Term Variations Of Surface Water Temperature In The Regions Of Mishima And Okinoshima (한국 근해 수온의 주기적 변화(II). 삼도와 중지도 해역 표면수온의 년주변화 및 장주기 변화)

  • Hahn, Sangbok
    • 한국해양학회지
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    • v.5 no.2
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    • pp.41-51
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    • 1970
  • Periodic characters of water temperature in the regions of the Mishima and the Okinoshima were derived through the analysis of the five days interval data during 1914 to 1970 mainly. In terms of ten days mean temperatures, annual variation function of the Mishima region, Korea Strait, is F($\theta_d$)=17.45-5.34 cos $\theta_d$-3.77 sin $\theta_d$+0.62 sin $2\theta_d$ -0.52 sin $3\theta_d$, where $\theta_d$=$\frac{\pi}{18}$(d-2), d is the order of ten days period 1 to 36. And in the region of Okinoshima, Tsushima Strait, we find F($\theta_d$)=18.88-5.39 cos $\theta_d$-3.60 sin $\theta_d$+0.52 sin $2\theta_d$. The annual mean temperature is 17.4$^{\circ}C$ in the Mishima region, 18.9$^{\circ}C$ in the Okinoshima region, and the amplitudes of annual variation functions are 7$^{\circ}C$ in both regions with minimum temperature in the middle ten days of February, maximum in the middle ten days of August. The long term variations of surface water temperature with 12 5 years period were observed in the annual mean temperature, monthly mean temperatures and the fixed day temperatures of every year. In addition to these, relatively short term variations were also found significant periods of 3 years, 4 years and 2 years, respectively.

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The High-Speed Extraction of Interest Region in the Parcel Image of Large Size (대용량 소포영상에서 관심영역 고속추출 방법에 관한 연구)

  • Park, Moon-Sung;Bak, Sang-Eun;Kim, In-Soo;Kim, Hye-Kyu;Jung, Hoe-Kyung
    • The KIPS Transactions:PartD
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    • v.11D no.3
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    • pp.691-702
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    • 2004
  • In this paper, we propose a sequence of method which extrats ROIs(Region of Interests) rapidly from the parcel image of large size. In the proposed method, original image is spilt into the small masks, and the meaningful masks, the ROIs, are extracted by two criterions sequentially The first criterion is difference of pixel value between Inner points, and the second is deviation of it. After processing, some informational ROIs-the areas of bar code, characters, label and the outline of object-are acquired. Using diagonal axis of each ROI and the feature of various 2D bar code, the area of 2D bar code can be extracted from the ROIs. From an experiment using above methods, various ROIs are extracted less than 200msec from large-size parcel image, and 2D bar code region is selected by the accuracy of 100%.