• Title/Summary/Keyword: D1-D2 region

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간흡충 세르카리아의 Chaetotaxy에 관한 연구 (Cercarial Chaetotaxy of Clonoychis sinensis)

  • 민득영;김재진
    • Parasites, Hosts and Diseases
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    • 제26권2호
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    • pp.113-116
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    • 1988
  • 간흡충 세르카리아의 감각유두 분포양상 즉 chaetotaxy를 관찰하였다. 이 세르카리아의 chaetotaxy는 두부(cephalic region)에서 제1열에 5∼6 Ci 1, 4∼5 Ci 2, 5∼6 Ci 3, 제2열에 4 Cii 1, 2 Cii 2, 4 Cii 3, 5∼6 Cii 4, 제3열에 3∼4 Ciii 1, 2∼3 Ciii 2, 및 제 4열에 2 Civ 1, 2∼3 Civ 2, 2∼3 Civ 3의 분포를 보였으며, 몸통 부위 (body region)의 각측(V) 및 때측(D)에서 2 AiV, 1 AiD, 2 AiiV, 1 AiiB,2 kiiiv,2 AiiiD, 1 AivV, 1 AivD, 1 PiiD, 1 PiiiD, 그리고 꼬리 부위(caudal region)에서 2-2-2-2의 분포양상을 보였다. 이 결과를Opisthorchis chabaudi 및 Metagonimus yokugawai에서 보고된 chaetotaxy와 비교하였다.

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Blastobotrys illinoisensis, an Unrecorded Anamorphic Yeast Strain Isolated from the Gut of the Earthworm Eisenia fetida

  • Ji Yun Son;Myung Kyum Kim
    • 한국균학회지
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    • 제50권4호
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    • pp.373-381
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    • 2022
  • Strain E4, an unrecorded species of dimorphic fungi, was isolated from the gut of earthworms collected in Gyeonggi Province, South Korea. Nucleotide sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region revealed that this species is a member of the genus Blastobotrys, Blastobotrys illinoisensis. Strain E4 differed from its closest known species, B. mokoenaii and B. malaysiensis, by harboring 3-5 and 12-14 nucleotide substitutions in the D1/D2 and ITS regions, respectively. Phylogenetic analysis based on concatenated sequences of the D1/D2 region of the LSU rRNA gene and the ITS region also indicated that strain E4 belongs to the Blastobotrys clade and is distinct from other related species in the clade. The previously unreported isolate could be distinguished from closely related species by its inability to ferment carbon sources. To our knowledge, this is the first report on the isolation of Blastobotrys species from the gut of earthworms in Korea. The strain used was E4 (=KCTC 27831=JCM 33428).

Metagonimus yokogawai 세르카리아의 감각유두에 관한 연구 (Study on Sensory Papillae of Metagonimus yokogawai Cercaria)

  • 김재진;민득영소진탁
    • Parasites, Hosts and Diseases
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    • 제22권1호
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    • pp.11-20
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    • 1984
  • A number of studies on the papillae of cercariae of trematodes reported that the papillar patterns (or chaetotaxy) of cercariae might be an excellent method to attain better understanding of the digenetic trematodes (Richard, 1971 ; Short and Cartrett, 1973; Bayssade-Dufour, 1979) . The present study was aimed to determine the number, distribution pattern and structure of the sensory papillae of Metagonimus yokogawai cercariae, and to elucidate the chaetotaxy of this digenetic trematode. M. yokogawai cercariae were pipetted from a vial in which infected snails (Semisulcospira libertina) had been kept for 3 hours. The snails were collected from an endemic area of M. yokogawai, Boseong river in west-southern part of Korea. Observations of papillae were based on light microscopy of those stained with silver nitrate, and on scanning electron microscopy The results are summarized as follows: 1, All papillae observed were uniciliated. 2. Cilia in anterior tip were shorter than the others in other portions. 3. The body papillae were arranged in essentially symmetrical patterns, Total number of the papillae was 126(63 pairs) in average; anterior tip 40(20 pairs), ventral 20(10 pairs), lateral 42(21 pairs), and caudal 8(4 pairs). 4. The chaetotany of M. yokogawai cercaria was: Ci cycle ($3+3C_{I}V,{\;}2+2C_{I}L,{\;}2+3C_{I}D),{\;}C_{II}{\;}cycle(2C_{II}V,{\;}1C_{II}L,{\;}2C_{II}D),{\;}C_{lll}{\;}cycle{\;}(1+lC_{III}V,{\;}1C_{IlI}L),{\;}C_{IV}{\;}cycle{\;}(1C_{IV}V,{\;}IC_{lV}L){\;}in{\;}cephalic{\;}region:{\;}A_I(1A_{IV}V,{\;}1+2A_{I}L,{\;}1A_{I}D),{\;}A_{II}(1A_{II}V,{\;}1+3A_{II}L,{\;}1A_{II}D),{\;}A_{III}(1A_{III}V,{\;}1+1A_{III}L,{\;}1A_{III}D){\;}and{\;}A_{IV}(1A_{IV}V,{\;}2A_{IV}L)$ in antacetabular region: $1M_{I}V{\;}and{\;}2M_{I}L$ in median: $1+1P_{I}L,{\;}1P_{II}L,{\;}1P_{II}D,{\;}1P_{III}L,{\;}1P_{IV}L{\;}and{\;}1P_{IV}D$ in postacetabular region: 2-2-2-2 in caudal region.

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Correlation between total air pollutant emissions and incidence of type 1 diabetes in the Russian Federation

  • Choi, Hoon Sung;Kim, Jin Taek;Seo, Ji-Young;Linkov, Faina;Shubnikov, Evgeniy;Lee, Hong Kyu
    • Clinical and Experimental Pediatrics
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    • 제64권10호
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    • pp.525-530
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    • 2021
  • Background: Exposure to air pollution (gaseous pollutants and/or particulate matter) has been associated with the incidence, prevalence, and mortality of type 1 diabetes (T1D). Purpose: To examine the quantitative relationship between air pollutant emissions and the incidence of T1D. Methods: We examined the association between the incidence of T1D and type 2 diabetes (T2D) in 2017 as well as that of T1D in patients younger than 15 years in 2016 with "emissions of air-polluting substances from stationary and mobile sources by regions of the Russian Federation in 2016" as reported by the Federal Diabetes Register of Russia downloaded from the Russian government website (http://www.mnr.gov.ru/docs/gosudarstvennye_doklady/o_sostoyanii_i_ob_okhrane_okruzhayushchey_sredy_rossiyskoy_federatsii/). Results: The incidence of T1D across all ages in each region of the Russian Federation correlated with the total air pollutants emitted in the region each year (r=0.278, P=0.013). The incidence of T2D was also correlated with the amount of air pollutants (r=0.234, P=0.037) and the incidence of T1D (r=0.600, P<0.001) in each country. Similarly, the incidence of T1D in patients younger than 15 years correlated with the total air pollutants emitted each year in each region (r=0.300, P=0.011). Conclusion: The quantitative relationship between the total air pollutants emitted and the incidence of T1D and T2D in the Russian Federation suggests that air pollution contributes to the development of T1D and T2D.

Internal transcribed spacer (ITS) region의 염기서열 분석에 의한 보길도산 황칠나무의 분자 계통학적 연구 (Phylogenetic Analysis of Dendropanax morbifera Using Nuclear Ribosomal DNA Internal Transcribed Spacer (ITS) Region Sequences)

  • 신용국
    • 생명과학회지
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    • 제26권11호
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    • pp.1341-1344
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    • 2016
  • 보길도에서 자라고 있는 황칠나무(Dendropanax morbifera)를 구입하여, 캘러스로 유도한 후, ribosomal DNA(nrDNA)의 internal transcribed spacer (ITS) region의 염기서열을 결정하였다 보길도의 황칠나무(Dendropanax morbifera)의 ITS region의 염기서열을 분석한 결과, 총 689염기를 결정하였다. 결정된 689염기 중에서 ITS1은 222 개염기, 5.8S rDNA는 160염기, ITS2는 233염기인 것으로 판명되었다. GenBank의 BLAST 프로그램(http://www.ncbi.nlm.nih.BLAST)을 사용하여 GenBank/EMBL/DDBJ에 등록되어 있는 Dendropanax 속 33의 염기서열을 수집한 후 multiple alignment를 수행한 결과, 유사도는 99.7%(D. chevalieri)에서 92.6%(Dendropanax arboreus)로 나타났으며, 일본황칠나무(D. trifidus)와는 유사도가 99.4%로 판명되었다.

A Fast Algorithm for Region-Oriented Texture Coding

  • Choi, Young-Gyu;Choi, Chong-Hwan;Cheong, Ha-Young
    • 한국정보전자통신기술학회논문지
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    • 제9권6호
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    • pp.519-525
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    • 2016
  • This paper addresses the framework of object-oriented image coding, describing a new algorithm, based on monodimensional Legendre polynomials, for texture approximation. Through the use of 1D orthogonal basis functions, the computational complexity which usually makes prohibitive most of 2D region-oriented approaches is significantly reduced, while only a slight increment of distortion is introduced. In the aim of preserving the bidimensional intersample correlation of the texture information as much as possible, suitable pseudo-bidimensional basis functions have been used, yielding significant improvements with respect to the straightforward 1D approach. The algorithm has been experimented for coding still images as well as motion compensated sequences, showing interesting possibilities of application for very low bitrate video coding.

FoxD2-AS1 is a prognostic factor in glioma and promotes temozolomide resistance in a O6-methylguanine-DNA methyltransferase-dependent manner

  • Shangguan, Wenbing;Lv, Xuyang;Tian, Nan
    • The Korean Journal of Physiology and Pharmacology
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    • 제23권6호
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    • pp.475-482
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    • 2019
  • Glioma is the most common brain tumor with a dismal prognosis. While temozolomide (TMZ) based chemotherapy significantly improves survival in glioma patients, resistance against this compound commonly leads to glioma treatment failure. Overexpression of long-noncoding RNA (LncRNA) FoxD2 adjacent opposite strand RNA 1 (FoxD2-AS1) was identified to promote glioma development, but the role in TMZ resistance remains unclear. In this paper, we found that FoxD2-AS1 was overexpressed in recurrent glioma, high FoxD2-AS1 expression was significantly correlated with poor patient outcome. Methylation of $O^6$-methylguanine-DNA methyltransferase (MGMT) is significantly less frequent in high FoxD2-AS1 expression patients. Knockdown of FoxD2-AS1 decreased the proliferation, metastatic ability of glioma cells and promote the sensitivity to TMZ in glioma cells. Furthermore, knockdown of FoxD2-AS1 induced hypermethylation of the promoter region of MGMT. Our data suggested that FoxD2-AS1 is a clinical relevance LncRNA and mediates TMZ resistance by regulating the methylation status of the MGMT promoter region.

A Comparison of the Ability of Fungal Internal Transcribed Spacers and D1/D2 Domain Regions to Accurately Identify Candida glabrata Clinical Isolates Using Sequence Analysis

  • Kang, Min-Ji;Choi, Yoon-Sung;Kim, Sunghyun
    • 대한의생명과학회지
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    • 제24권4호
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    • pp.430-434
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    • 2018
  • Candida glabrata is the second most prevalent causative agent for candidiasis following C. albicans. The opportunistic yeast, C. glabrata, is able to cause the critical bloodstream infections in hospitalized patients. Conventional identification methods for yeasts are often time consuming and labor intensive. Therefore, recent studies on sequence-based identification have been conducted. Recently, sequencing the D1/D2 domain of the large subunit ribosomal RNA gene and the internal transcribed spacers (ITS) 1 and ITS2 regions of the ribosomal DNA has proven useful for DNA-based identification of most species of fungi. In the present study, therefore, fungal ITS and D1/D2 domain regions were targeted and analyzed by DNA sequencing for the accurate identification of C. glabrata clinical isolates. A total of 102 C. glabrata clinical isolates from various clinical samples including bloodstream, catheterized urine, bile and other body fluids were used in the study. The results of the DNA sequence analysis showed that the mean standard deviation of species identity percent score between ITS and D1/D2 domain regions was $97.8%{\pm}2.9$ and $99.7%{\pm}0.46$, respectively. These results revealed that the D1/D2 domain region might be a better target for identifying C. glabrata clinical isolates based on DNA sequences than the ITS1 and ITS2 regions. However, in order to evaluate the usefulness of D1/D2 domain region for species identification of all Candida species, other Candida species such as C. albicans, C. tropicalis, C. dubliniensis, and C. krusei should be verified in further studies additionally.

한국 근해 수온의 주기적 변화(II). 삼도와 중지도 해역 표면수온의 년주변화 및 장주기 변화 (Periodic Variations Of Water Temperature In The Seas Around Korea(II). Annual And Long Term Variations Of Surface Water Temperature In The Regions Of Mishima And Okinoshima)

  • 한상복
    • 한국해양학회지
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    • 제5권2호
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    • pp.41-51
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    • 1970
  • 수권(Hydrosphere)은 기권(Atmosphere)과 암권(Lithosphere)의 중간에 있으며 이에 대한 연구는 지구 물리학 분야에서 가장 중요하게 다루어 지는 것 중의 하나이다. 특히 수권의 70% 이상을 차지하고 있는 해양에서의 환경 변화를 조사하는 것은 해황의 변동을 예측하여 수산업이나 기타의 해중 시설물을 설치하는 데 중요한 자료를 제공해 주는 기초 적인 분야이다.

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대용량 소포영상에서 관심영역 고속추출 방법에 관한 연구 (The High-Speed Extraction of Interest Region in the Parcel Image of Large Size)

  • 박문성;박상은;김인수;김혜규;정회경
    • 정보처리학회논문지D
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    • 제11D권3호
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    • pp.691-702
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    • 2004
  • 본 문은 고속으로 이송되는 컨베이어 벨트 환경에서 획득된 대용량 소포 영상에서 다양한 관심영역(ROI:Region of Interest)을 고속 추출할 수 있는 방법을 제안한다. 제안된 방법의 친 번째 단계에서는 영상을 32${\times}$32 픽셀 크기의 마스크로 나누고 그 중 내부 픽셀의 최대값과 최소값의 차이값이 최저 동적 입계값보다 큰 경우에 1차 후보로 검출한다. 두 번째 단계에서는 1차 후보 마스크를 다시 8${\times}$8크기의 서브마스크로 나누고 적셀 값의 편차(deviation)을 기준으로 최저 임계값보다 큰 마스크만 남김으로서 불필요한 영역을 제거한다. 이러한 전처리 과정을 거쳐 1차원 바코드, 2차원 바코드, 소포의 외형 경계, 레이블 및 문자와 같은 소포의 정보가 기록되어 있는 영역(ROI)을 제외한 나머지 영역을 제거한다. 후처리 과정에서는 위의 ROI 중 2차원 바코드 영역만을 추출하기 위해서 각각의 ROI에 대하여 기준축을 생성한 결과를 이용하여 각각의 영역을 검증하였다. 이와 같은 방법으로 실험한 결과 대용량 소포영상에서 약 200msec 이내에 다양한 ROI를 추출하였으며 100%의 정확도로 2차원 바코드 영역을 지정할 수 있음을 확인하였다.