• 생명과학회지
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• 제27권1호
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• pp.95-99
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• 2017

우리는 이전실험에서 배양세포를 사용하여 4종류의 천연물(Corydaline, (${\pm}$)-Car-3-ene-2,5-dione, Cinobufagin, Corilagin)이 ERAP1와 FOXO1 (DFA16) 유전자발현을 2배 이상 상승시키는 것을 증명하였다. 본 실험은 1% agar, 5% sucrose, natural compound $20{\mu}l$를 넣은 먹이를 만들어 4시간 starvation후에 4시간 동안 먹였다. Cinobufagin와 Corilagin를 먹이면 대조군에 비하여 6-8일 정도 더 생존하였다. RT-PCR 실험결과 ERAP1와 FOXO1 유전자 발현을 조절하는 것이 증명되었다. 산업곤충질병 진단과 치료에 사용될 것이며, 초파리를 대신한 생쥐에서도 동일한 결과를 실험하여 수명연장을 위한 신약으로 발전시킬 것이다.

• 생명과학회지
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• 제22권11호
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• pp.1558-1570
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• 2012

방사선 생물학에서 방사선에 대한 반응으로 매개되는 다양한 기작에 대한 분석을 위해 여러 종류의 모델 생물체를 사용해 왔다. 모델 생물체는 생물학적으로 온전한 in vivo 환경을 제공할 수 있기 때문에 방사선에 의해 발생되는 세포 내 현상은 물론 생리적인 현상이나 병리학적인 현상을 규명하는 데 있어서 모델 생물체를 사용하는 것은 효과적인 방법이 될 수 있다. 지금까지 축적된, 모델 생물체를 이용한 방사선 생물학적 연구결과들은 새로운 방사선치료 보조제의 개발, 방사선치료 효율 증진 등에 적용되어 여러 질병에 대한 임상연구의 기초가 되어왔다. 이렇게 유용하게 사용된 여러 모델 생물체에 있어서, 각각의 모델에 대한 개별적인 정보에 대한 연구는 다양한 방면에서 이루어지고 있지만, 통합적인 비교, 분석 및 정리를 한 경우는 부족한 실정이다. 따라서, 본 논문에서는 방사선 생물학에서 지금까지 많이 사용된 모델 생물체 4종(효모, 예쁜꼬마선충, 초파리, 생쥐)에 대해 각 생물체가 갖는 모델로써의 특징과 장단점 그리고 방사선 생물학 연구에 이용된 사례 등을 서술하고자 한다.

• International Journal of Industrial Entomology and Biomaterials
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• 제3권2호
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• pp.121-126
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• 2001

A cDNA encoding a cathepsin D homologue was cloned from a cDNA library of the mulberry longicorn beetle, Apriona germari. Sequence analysis of the cDNA encoding the cathepsin D homologue of A. germari revealed that the 1,158 bp cDNA has an open reading frame of 386 amino acid residues. The deduced protein sequence of the A. germari cathepsin D homologue shows high homology with cathepsin D in insects, Aedes aegypti (68.2% amino acid similarity) and Drosophila melanogaster (67.2% amino acid similarity). Two aspartic residues and six cystein residues in the A. germari cathepsin D homologue are present at identical locations in all of the other catepsins D. Unlike cathepsins D in two insect species, A. gemari cathepsin D homologue appears to have two putative glycosylation sites, rather than one. Phylogenetic analysis revealed the A. germari cathepsin D homologue is more closely related to insect cathepsins D than to the other animal cathepsins D. Northern blot analysis suggests that A. germari cathepsin D homologue gene is expressed in most if not all, body tissues.

• International Journal of Industrial Entomology and Biomaterials
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• 제9권1호
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• pp.127-130
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• 2004

We describe here the cloning and characterization of a cDNA encoding a putative myosin light chain-2 (MLC-2) from the mole cricket, Gryllotalpa orientalis. The G. orientalis MLC-2 cDNA sequences comprised of 615 bp with 205 amino acid residues with a calculated molecular weight of approximately 23 kDa. The deduced protein sequence of G. orientalis MLC-2 cDNA showed 64% and 54% identity to Drosophila melanogaster MLC-2 and D. yakuba MLC-2, respectively. Northern blot analysis confirmed the muscle-specific expression of G. orientalis MLC-2.

• Interdisciplinary Bio Central
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• 제5권3호
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• pp.6.1-6.7
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• 2013

Many insects such as mosquitoes cause life-threatening diseases such as malaria, yellow fever and West Nile virus. Malaria alone infects 500 million people annually and causes 1-3 million death per year. Volatile insect repellents, which are detected through the sense of smell, have long been used to protect humans against insect pests. Antifeed-ants are non-volatile aversive compounds that are detected through the sense of taste and prevent insects from feeding on plants. The molecular targets and signaling path-ways required for sensing insect repellents and antifeedants are poorly understood. Transient Receptor Potential (TRP) Ca2+-permeable cation channels exist in organisms ranging from C. elegans to D. melanogaster and Homo sapiens. Drosophila has 13 family members, which mainly function in sensory physiology such as vision, thermotaxis and chemotaxis. G protein-coupled receptors (GPCRs) initiate olfactory signaling cascades in mammals and in nematodes C.elegans. However, the mechanisms of G protein signaling cascades in insect chemosensation are controversial. In this review, I will discuss the putative roles of G protein-coupled receptors (GPCRs) and Transient Receptor Potential (TRP) channels as targets for insect repellents.

• 한국동물학회지
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• 제34권1호
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• pp.123-130
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• 1991

Several parameters of u -glycerol-3-pholphate dehydrogenase (GPDH) such as activity, content and translatable mRNA levels were measured to elucidate mechanism underlving developmental and tissue specific regulation of 6PDH activity in Drosophila melonogastrr. In adult segments, most of total GPDH activity (62%1 Iwas detected in thorax where GPDH-1 resided, while 32% of total GPDH aUiviD was only detected in abdomen where GPDH-3 resided. The relative synthesis of GPDH was, however, similar in both tissues, although 58% of total GPDH was synthesized in abdomen. These results strongly suggest that the turnover rate of the abdominal enzyme (GPDH-3) was much more rapid than that of thoracic enzymes (GPDH-1). In nitro translation and immunoblotting experiments also indicate that GPDH-3 was arised by posttranslational modification from a single polypeptide (GPDH-1).

• 한국동물학회지
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• 제7권2호
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• pp.41-47
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• 1964

D.auraria is a species belonging to the D.melanogaster group, and this species was divided into 3 races(A, B and C race ) by morphoogical difference of the genitals . Korean populations of D.auraria A race are polymorphic with respect to the pigmentation of the abdominal tergites. The female shows two forms of color pattern, dark and light, on the sixth to the ninth tergites, and the male has no distinction shown by the female. Crossing experiments in the laboratory have shown that the difference between these color forms is due to a single pair of allelic genes located on an autosome. In natural populations, the light forms of females are always commoner than the dark one. The number of the light form increases relatively in southern localities and decreases relatively in northern localities. Furthermore, the number of the light form increases relatively in summer and decreases relatively in fall and spring. By genetic analyses of these wild flies, the relation, d/d>d/D>D/D has been disclosed as regards the relative frequencies of the genotypes in natural populations. Three experimental populations have been set up in the laboratory. Homozygous dark forms and homozygous light forms were mixed together and cultured in population cage at the temperature of 25 $^{\circ}C$. Approximately one year later, the frequencies of the light and the dark forms reached an equilibrium , the light one being usually more frequent than the dark one. This indicates that the heterozygous dark form possesses the highest adaptive value, the homozygous light being intermediate and the homozygous dark lowest. In number of adults hached, the homozygous light form was superior to the heterozygous dark form and the homozygous dark form, but the differences are scarcely significant.

• Molecules and Cells
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• 제26권6호
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• pp.576-582
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• 2008

Nervous system development takes place after positional information has been established along the dorsal-ventral (D/V) axis. The initial subdivision provided by a gradient of nuclear dorsal protein is maintained by the zygotic genes expressed along the D/V axis. In this study, an investigation was conducted to determine the range of Dpp function in repressing the expression of eagle (eg) that is present in intermediate neuroblasts defective (ind) and muscle specific homeobox (msh) gene domain. eg is expressed in neuroblast (NB) 2-4, 3-3 and 6-4 of the msh domain, and NB7-3 of the ind domain at the embryonic stage 11. In decapentaplegic (dpp) loss-of-function mutant embryos, eg was ectopically expressed in the dorsal region, while in dpp gain-of-function mutants produced by sog or sca-GAL4/UAS-dpp, eg was repressed by Dpp. It is worthy of note that Dpp produced from sim;;dpp embryos showed that Dpp could function at long range. However, Dpp produced from en-GAL4/UAS-dpp or wg-GAL4/UAS-dpp primarily acted at short-range. This result demonstrated that this discrepancy seems to be due to the repression of Dpp to EGFR signaling in sim;;dpp embryos. Taken together, these results suggest that Dpp signaling works at short-range, but can function indirectly at long-range by way of repression of EGFR signaling during embryonic neurogenesis.

• International Journal of Industrial Entomology and Biomaterials
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• 제2권1호
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• pp.1-6
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• 2001

This paper reports a few characteristics of seven insect mitochondrial genomes sequenced completely (Bombyx mori, Drosophila melanogaster, D. yakuba, Apis mellifera, Anopheles gambiae, A. quadrimaculatus, and Locusta migratoria). Comparative analysis of complete mt genome sequences from several species revealed a number of interesting features (base composition, gene content, A+T-rich region, and gene arrangement, etc) of insect mitochondrial genome. The properties revealed by our work shed new light on the organization and evolution of the insect mitochondrial genome and more importantly open up the way to clearly aimed experimental studies for understanding critical roles of the regulatory mechanisms (transcription and translation) in mitochondrial gene expression.

• Bulletin of the Korean Chemical Society
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• 제30권2호
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• pp.323-326
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• 2009

SR proteins are essential splicing regulators and also modulate alternative splicing events, which function both as redundant and substrate-specific manner. The Drosophila B52/SRp55, a member of the SR protein family, is essential for the fly development in vivo, as deletion of B52 gene results in lethality of animals at the second instar larval stage. Identification of the splicing target genes of B52 thus should be crucial for the understanding of the specific developmental role of B52 in vivo. In this study, we performed whole-genome DNA microarray experiments with a B52- knock-out animal. Analysis of the microarray data not only provided the B52-dependent gene expression signature, but also revealed a larval-stage specific, alternative splicing target gene of B52. Our result thus provides a starting point to understand the essential function of B52 at the organismal level.