• 제목/요약/키워드: D.B.H. analysis

검색결과 1,378건 처리시간 0.037초

Conformational Studies of Macrocyclic Corrin-Ring of Coenzyme B12 by NMR methods

  • Kim, Daesung;Park, Jung-Rae;Hoshik Won
    • 한국자기공명학회논문지
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    • 제3권1호
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    • pp.44-51
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    • 1999
  • An enzyme derived conformational changes of cobalamine is thought to be important in the homolytic cleavage of Co-C bond which is the first step of catalytic Cl-cycle of coenzyme B12-dependent enzymes. Modern 2D-NMR and NMR-based distance geometric studies were carried out to determine the 3D structure of corrin ring. Homonuclear and heteronuclear correlation NMR experiments were performed for complete 1H-NMR signal assignments. Distances between numerous proton pairs were deduced based on the NOE cross peak intensities and subsequently used as input into the distance geometry program for the 3D structure determination. The detailed 3D structure from the present NMR-based analysis was compared with the result from X-ray crystallographic study, which revealed greater conformational changes occur in benzimidazole group and sugar ring than in macrocyclic corrin and tetrapyrrole. In addition, the distance geometry used in this study was found to be quite useful for NMR-based structure determination of medium-sized molecules that give poor NOE effects arising from their intermediate tumbling rate ($\omega$$\tau$c 1.0).

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도시철도용 구동기어의 설계 요소에 대한 소음 기여도 분석 (Noise Source Identification of the Design Elements of the Driving Gear for the Urban Railway)

  • 김기남;이현철;선찬웅;이성욱
    • 한국소음진동공학회논문집
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    • 제25권7호
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    • pp.470-480
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    • 2015
  • As the railway noise guideline of the Ministry of Environment after 2017 is strictly enforced, the noise level at stationary condition of urban railway is demanding the reduction about 2 dB(A). And the noise level at running condition is reduced by 6 dB(A) at 80 km/h. Therefore, the devices that causes noise shall arrange for the improvement plan of noise reduction for each device. In this paper, we carried out a technical review of the driving gear used to drive the vehicle from a variety of noise-induced equipment of a urban railway. Analyze the causes of the current noise levels and noise cause about the driving gear used in current urban railway and this study analyzes the noise level and noise cause the drive gear being used in the current urban railway. Finally, in this paper proposes a scheme for reducing the noise that can be designed to reduce the noise with considering the noise cause analysis.

LNG 저장탱크 Roof 설계에 관한 연구 (The Study of Roof Design for LNG Storage Tank)

  • 홍성호;이강원;오병택;김영균;김기범
    • 한국가스학회지
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    • 제6권1호
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    • pp.32-37
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    • 2002
  • 천연가스는 청정 무공해 에너지라는 장점으로 소비량이 매년 크게 증가하고 있는 주요 에너지원이다 천연가스 수요의 증가에 따른 LNG 저장 시설의 확충이 요구되고 있어, 한국가스공사에서는 LNG 저장탱크 설계 기술을 개발하고 있다. 본 논문에서는 유한요소법을 이용하여 LNG 저장탱크의 Roof를 설계에 대하여 연구하였으며, 설계 및 평가에 적용된 하중 조건과 안전 코드는 BS7777 코드에 따라 연구하였다.

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Alloimmune and Skin Allograft Responses In 4-1BB (CD137)-deficient Mice

  • Wolisi, Godwin;Srirangam, Anjaiah;Vinay, Dass S.;Suh, Jae H.;Suh, Ho-Seok;Choi, Beom K.;Kwon, Byoung S.
    • IMMUNE NETWORK
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    • 제2권3호
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    • pp.133-136
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    • 2002
  • Background: The costimulatory molecule 4-1BB, a member of nerve growth factor receptor/tumor necrosis factor (NGFR/TNFR) super family, is involved in cell survival and death. Methods: In this study, female C57BL/6 ($H-2^b$) mice were used as a recipient, and DBA/2 ($H-2^d$) as a donor to assess a mixed lymphocyte reaction (MLR) and CTL response in vitro, and skin graft survival. IL-2, IFN level was measured by ELISA. Results: Mixed lymphocyte reaction (MLR) analysis showed that 4-1BB-deficient responder cells showed enhanced cellular proliferation over littermate controls. In contrast, IL-2 production was diminished only in 4-1BB knockout cultures. The IFN expression, on the other hand, was comparable between the groups. When female C57BL/6 ($H-2^b$) mice were grafted with the trunk skin of DBA/2 ($H-2^d$) mice, the in vivo tissue destruction of 4-1BB-deficient mice was not distinct from the normal littermates. Conclusion: These data suggest that 4-1BB is critical for the induction of alloreactive responses in vitro but 4-1BB alone could not change the course of skin rejection in vivo.

GIS 예방진단 시스템을 위한 정시간성 보장형 통신기기 개발 (Development on Communication Device with Timeliness Guaranteed for "GIS Preventive and Diagnosis System")

  • 민병운;명희철;최호웅;박창선;김정한;이병호;박동호;김윤관;이동철
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2007년도 제38회 하계학술대회
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    • pp.2032-2033
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    • 2007
  • There has been increasing interests of condition monitoring and diagnosis for electric equipment, which lead to the development of this system domestic and abroad. In the past, operators' interest was how to quickly repair and restore the electric equipments after failure. But, due to the North American Blackout in 2003 and the aging of equipments, users have paid attention to the condition based monitoring of electric equipment to prevent a fault like outrage. GIS-PDS("GIS Preventive and Diagnosis System") requiresa large amount of measurement data with timeliness for monitoring and analysis of real-time state of GIS(Gas Insulated Switchgear). We developed the timeliness-guaranteed communication device for GIS-PDS.

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하니컴 샌드위치 Panel을 이용한 LCD/PDP생산공정용 고기능성 복합 신소재 파렛트의 최적설계 (The Optimum Design of the Light-weight Composite Pallet Plank for Assembly Line of LCD/PDP by using Honeycomb Sandwich Panel)

  • 김윤해;최병근;손진호;조영대;엄수현;우병훈
    • 한국마린엔지니어링학회:학술대회논문집
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    • 한국마린엔지니어링학회 2005년도 전기학술대회논문집
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    • pp.388-394
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    • 2005
  • A typical honeycomb sandwich panel consists of two thin, high-strength facings bonded to a thick, light-weight core. Each component by itself is relatively weak and flexible, but when it combind in a sandwich panel they produce a structure that is stiff, strong, and lightweight. In addition to use in honeycomb sandwich panels, honeycomb is used for energy absorption, radio frequency shielding, light diffusion, and to direct air flow.Accordingly, the usage of honeycomb sandwich structure is very widely applied to the aircraft, the automobile, and marine industry, etc., because of these advantages. Generally, this honeycomb sandwich structure is manufactured by autoclave process.In this study, the honeycomb sandwich structure was produced by prepreg. To prove the suitability the honeycomb sandwich structure with prepreg, The optimum design of the skin materials and honeycomb sandwich structure were evaluated with the theory of stress analysis.

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Tragacanth gum 의 신다당류(新多糖類) C 의 화학구조(化學構造) - Tragacanth gum의 신다당류(新多糖類)에 관(關)한 연구(硏究) 제2보(第二報) - (Studies on the Chemical Structure of the New Polysaccharide C - (The New Polysaccharides of Gum Tragacanth. II) -)

  • 이성환
    • Applied Biological Chemistry
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    • 제3권
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    • pp.25-48
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    • 1962
  • tragacanth gum의 화학구조(化學構造)를 구명(究明)하기 위(爲)하여 미국(美國) 약전(藥典)의 tragacanth gum 분말(粉末)을 가지고 다음의 실험(實驗)을 통(通)하여 이의 성분(成分)의 하나인 polysaccharide C를 분리(分離)하여 이의 화학구조(化學構造)를 밝혔다. (1) tragacanth gum을 85% 주정(酒精)으로 처리(處理)해서 중성다당류(中性多糖類)로 polysaccharide C를 분리(分離)하였으며 구성당(構成糖)으로 L-rhamnose, D-xylose, L-arabinose 및 D-galactose를 paper chromatography와 Cellulose column chromatography로 분리(分離), 동정(同定)하였다. 이의 molar ratio는 2:1:17:9이며 비선광도(比旋光度)는 $[{\alpha}]^{30}_D-72.2이다. (2) 구성당(構成糖)의 결합위치(結合位置)를 구명(究明)하기 위(爲)해 Hawarth 법(法)과 Purdietldir(試藥)을 가지고 methyl화(化)시켜 methyl화(化) polysaccharide C를 얻었으며 비선광도(比旋光度) $[{\alpha}]^{22}_D-102를 보였다. 이것을 가수분해(加水分解)시켜 paper chromatography와 column chromatography를 통(通)해 methyl화단당(化單糖)으로 1,3,5-tri-O-methyl-L-arabofuranose, 3,4-di-O-methyl-L-rhamnose, 2,3-di-O-methyl-D-xylose, 2,3,4-tri-O-methyl-D-galactopyranose, 2,4-di-O-methyl-L-arabopyronose, 2,4-di-O-methyl-D-galactose, 2-O-methyl-L-arabinose 및 L-arabinose를 분리(分離), 동정(同定)하였다. (3) 산(酸)의 각종농도(各種濃度)에 따른 부분적(部分的) 가수분해(加水分解)를 시켜 polysaccharide C의 end group, 측지(側枝) 또는 주쇄(主鎖)를 이루는 구성당(構成糖)을 밝히기 위(爲)하여 0.05 N-HCl로 제1차(第一次) 가수분해(加水分解). 0.1N-HCl로 제2차(第二次) 가수분해(加水分解), 0.3N-HCl로 제3차(第三次) 가수분해(加水分解)를 하여 가수분해물(加水分解物)과 비가수분해물(非加水分解物)에서 각각(各各) 다음과 같은 구성단당(構成單糖)을 검출(檢出)하고 이들의 molar ratio를 측정(測定)하였다. 제1차(第一次) 가수분해물(加水分解物)(A)에서 L-arbinose, 비가수분해물(非加水分解物)(A')에서 L-rhamnose, D-xylose, L-arabinose 및 D-galactose; 제2차(第二次) 가수분해물(加水分解物)(B)에서 L-arabinose와 D-galactose, 비가수분해물(非加水分解物)(B')에서 L-rhamnose, D-xylose, L-arabinose, 및 D-galactose; 제3차(第三次) 가수분해물(加水分解物)(C)에서 L-rhamnose, D-xylose, L-arabinose 및 D-galactose, 비가수분해물(非加水分解物)(C')에서 D-xylose와 D-galactose를 검출(檢出)하였다. (4) 구성당(構成糖)의 형태(形態)와 구조(構造)를 밝히기 위(爲)해 polysaccharide C에 대한 periodate산화(酸化) 실험(實驗)을 하여 $C_5H_8O_4$당(當) periodate의 소비(消費)와 formic acid의 생성량(生成量)을 측정(測定)하였는데 periodate의 소비량(消費量)은 1.23 mole, formic acid의 생성량(生成量)은 0.78 mole이다.

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강원도산(江原道産) 소나무천연림생태계(天然林生態系)의 Biomass 및 Net Primary Production에 관(關)한 연구(硏究) (Biomass and Net Primary Productivity of Pinus densiflora Natural Ecosystem in Kangwondo, Korea)

  • 이수욱
    • 한국산림과학회지
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    • 제71권1호
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    • pp.74-81
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    • 1985
  • 강원도산(江原道産) 소나무천연림(天然林)의 생산성(生産性)을 파악(把握)하기 위하여 10본(本)의 표본목(標本木)을 벌도(伐倒)하고 각(各) 부위별(部位別) 및 총(總) biomass와 biomass 추정방정식(推定方程式) 모형으로는 $Wt=aD^bH^c$가 가장 적합(適合)하였다. 2) 임분(林分) biomass 추정량(推定量)은 지상부(地上部) 총량(總量)이 198.82 t/ha였으며 부위별(部位別)로는 수간목부(樹幹木部)가 136.82 t/ha, 생지부(生枝部)가 32.81 t/ha, 수피부(樹皮部)가 10.72 t/ha 엽량(葉量)이 9.07 t/ha 죽은가지가 7.30 t/ha 였다. 3) 순생산량(純生産量)은 지상부총량(地上部總量)이 15.87 t/ha/yr였으며 부위별(部位別)로는 수간목부(樹幹木部)가 7.04 t/ha/yr., 생지부(生枝部)가 4.91 t/ha/yr., 엽량(葉量)이 2.21 t/ha/yr., 소지(小枝)가 1.13 t/ha/yr., 수피(樹皮)가 0.52 t/ha/yr 였다. 4) Leaf efficiency는 1.876으로서 연간지상부(年間地上部) 총생산량(總生産量) 15.87 t/ha/yr와 비교적 잘 연관되어 있다.

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완충재의 동특성에 따른 중량충격음 증폭에 관한 해석적 연구 (The Effect of Dynamic Property of Absorbing Sheet on the Amplification of Heavy Weight Floor Impact Noise)

  • 황재승;문대호;박홍근;홍성걸;홍건호
    • 한국소음진동공학회논문집
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    • 제20권7호
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    • pp.651-657
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    • 2010
  • Previous experimental results performed by many researchers for a couple of decades in South Korea have shown that an absorbing sheet inserted in a conventional floating slab system for thermal insulation or vibration absorption may amplify the vibration of the slab system at specific frequency ranges depending on the material properties of the sheet. The amplified vibration, consequently, results in the heavy weight floor impact noise exceeding the sound level limit for an apartment house, 50 dB. In this study, the amplification mechanism is examined through numerical analysis and a new slab system is proposed to reduce the amplification and control the noise. The new slab system consists of studs connecting the base slab and upper concrete finishing yielding the dramatically increased stiffness of the slab. The numerical simulation is performed to investigate the effect of the slab system with studs on the vibration and noise control. The results show that the performance of the slab is sensitive to the number and location of studs, and the heavy weight floor impact noise can be reduced up to 6~7 dB compared to the conventional slab system at the optimal stud location.

Protein Profile in Corpus Luteum during Pregnancy in Korean Native Cows

  • Chung, H.J.;Kim, K.W.;Han, D.W.;Lee, H.C.;Yang, B.C.;Chung, H.K.;Shim, M.R.;Choi, M.S.;Jo, E.B.;Jo, Y.M.;Oh, M.Y.;Jo, S.J.;Hong, S.K.;Park, J.K.;Chang, W.K.
    • Asian-Australasian Journal of Animal Sciences
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    • 제25권11호
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    • pp.1540-1545
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    • 2012
  • Steroidogenesis requires coordination of the anabolic and catabolic pathways of lipid metabolism, but the profile of proteins associated with progesterone synthesis in cyclic and pregnant corpus luteum (CL) is not well-known in cattle. In Experiment 1, plasma progesterone level was monitored in cyclic cows (n = 5) and pregnant cows (n = 6; until d-90). A significant decline in the plasma progesterone level occurred at d-19 of cyclic cows. Progesterone level in abbatoir-derived luteal tissues was also determined at d 1 to 5, 6 to 13 and 14 to 20 of cyclic cows, and d-60 and -90 of pregnant cows (n = 5 each). Progesterone level in d-60 CL was not different from those in d 6 to 13 CL and d-90 CL, although the difference between d 6 to 13 and d-90 was significant. In Experiment 2, protein expression pattern in CL at d-90 (n = 4) was compared with that in CL of cyclic cows at d 6 to 13 (n = 5). Significant changes in the level of protein expression were detected in 32 protein spots by two-dimensional polyacrylamide gel electrophoresis (2-DE), and 23 of them were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Six proteins were found only in pregnant CL, while the other 17 proteins were found only in cyclic CL. Among the above 6 proteins, vimentin which is involved in the regulation of post-implantation development was included. Thus, the protein expression pattern in CL was disorientated from cyclic luteal phase to mid pregnancy, and alterations in specific CL protein expression may contribute to the maintenance of pregnancy in Korean native cows.