• IMMUNE NETWORK
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• v.8 no.1
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• pp.21-28
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• 2008

Background: A co-inhibitory molecule, B7-H4, is believed to negatively regulate T cell immunity by suppressing T cell proliferation and inhibiting cytokine production. However, the mechanism behind B7-H4-mediated tolerance remains unclear. Methods: Balb/c $(H-2^d)$ mice were fed with dendritic cell line, DC2.4 $(H-2^d)$ every day for 10 days. Meantime, mice were hydrodynamically injected with recombinant plasmid expressing B7-H4 fusion protein (B7-H4.hFc) or hFc via tail vein. One day after last feeding, mice were immunized with allogeneic B6 spleen cells. 14 days following immunization, mice were challenged with B6 spleen cells to ear back and the ear swelling was determined the next day. Subsequently, a mixed lymphocyte reaction (MLR) was also performed and cytokines profiles from the reaction were examined by sandwich ELISA. Frequency of immunosuppressive cell population was assayed with flow cytometry and mRNA for FoxP3 was determined by RT-PCR. Results: Tolerant mice given plasmid expressing B7-H4.hFc showed a significant reduction in ear swelling compared to control mice. In addition, T cells from mice given B7-H4.hFc plasmid revealed a significant hyporesponsiveness of T cells against allogeneic spleen cells and showed a significant decrease in Th1 and Th2 cytokines such as IFN-${\gamma}$, IL-5, and TNF-${\alpha}$. Interestingly, flow cytometric analysis showed that the frequency of CD4+CD25+FoxP3+ Tregs in spleen was increased in tolerant mice given recombinant B7-H4.hFc plasmid compared to control group. Conclusion: Our results demonstrate that B7-H4 synergistically potentiates oral tolerance induced by allogeneic cells by increasing the frequency of FoxP3+ CD4+CD25+ Treg and reducing Th1 and Th2 cytokine production.

• Food Science of Animal Resources
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• v.35 no.3
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• pp.360-369
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• 2015

Milk proteins have many potential sequences within their primary structure, each with a specific biological activity. In this study, we compared and investigated the bioactivities of hydrolysates of the domestic (A, B) and imported (C, D) skim milk powders generated using papain digestion. MALDI-TOF analysis revealed that all milk powder proteins were intact, indicating no autolysis. Electrophoretic analysis of hydrolysates showed papain treatment caused degradation of milk proteins into peptides of various size. The antioxidant activity of the hydrolysates, determined using 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and total phenolic contents (TPC) assays, increased with incubation times. In all skim milk powders, the antioxidant activities of hydrolysates were highest following 24 h papain treatment (TPC: A, 196.48 μM GE/L; B, 194.52 μM GE/L; C, 194.76 μM GE/L; D, 163.75 μM GE/L; ABTS: A, 75%; B, 72%; C, 72%; D, 57%). The number of peptide derived from skim milk powders, as determined by LC-MS/MS, was 308 for A, 283 for B, 208 for C, and 135 for D. Hydrolysate A had the highest antioxidant activity and the most potential antioxidant peptides amongst the four skim milk powder hydrolysates. A total of 4 β-lactoglobulin, 4 α_s1-casein, and 56 β-casein peptide fragments were identified as potential antioxidant peptides in hydrolysate A by LC-MS/MS. These results suggest that domestic skim milk could have applications in various industries, i.e., in the development of functional foods.

• Proceedings of the KIEE Conference
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• summer
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• pp.527-529
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• 2004

This paper researched leakage current characteristics of EHW insulators(36,000lbs) to artificially contaminated clay and kaolin through construction of long-periods testing facility. Insulators were contaminated and classified into the ESDD level(B, C and D) under IEC Pub. 60507 standards method. As a result of tests, leakage current of the heavily contaminated D level to clay was approximately 2,260 $[\muA]$, which is contaminated to kaolin was approximately 3,200 $[\muA]$, during initial rainfall. However after raining, leakage current of naturally washed contamination insulators was lower at approximately 300 $[\muA]$. As regular temperature $20^{\circ}C$, leakage current increased greatly in case humidity density was over $72\%$. But, leakage current was not influenced by temperature variation in case of regular $\%$ humidity.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.7
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• pp.927-930
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• 2006

This study was conducted to investigate potential effects of previously identified QTL regions on carcass traits in Hanwoo. The data analyzed in this study was collected from 326 steers of 67 proven sire. Thirteen micorsatellite markers spanning QTL regions on bovine chromosomes 1 and 14 were genotyped in 326 steers. The following breeding values were analyzed for QTL effects. Cold carcass weight breeding value (CCWBV), longissimus muscle area breeding value (LMABV), marbling score breeding value (MSBV) and backfat thickness breeding value (BFTBV). Chi-square tests were performed to compare frequencies of individual allele between high and low breeding value groups. Significant differences of allele frequencies in BMS711, MCM130, BMS4049, and BMS2263 were found. And also, in RM180, BL1029, BM4305, and BMS2055 there were significant differencies of allele frequencies. These results showed a potential application for investigation of putative QTL locations.

• The KSFM Journal of Fluid Machinery
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• v.5 no.4 s.17
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• pp.32-39
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• 2002

The purpose of this 3-D numerical simulation is to calculate and examine the complex 3-D stall phenomena on the rotor blade and wake distribution of the wind turbine. The flow characteristics of 500kW Horizontal Axis Wind Turbine (HAWT) are compared with the calculated 3-D stall phenomena and wake distribution. We used the CFX-TASCflow to predict flow and power characteristics of the wind turbine. The CFD results are somewhat consistent with the BEM (Blade Element Momentum) results. And, the rotational speed becomes faster, the 3-D stall region becomes smaller. Moreover, the pressure distribution on the pressure side that directly gets the incoming wind grows high as it goes toward the tip of the blade. The pressure distribution on the blade's suction side tells us that the pressure becomes low in the leading edge of the airfoil as it moves from the hub to the tip. However, we are not able to precisely predict on the power coefficient of the rotor blade at the position of generating complex 3-D stall region.

• Journal of the Korea institute for structural maintenance and inspection
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• v.19 no.1
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• pp.72-80
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• 2015

In this study, comparative analysis has been performed with regard to a bending stress and deformation at bottom slab of a concrete floating container terminal by live load distributions. In addition, a structural performance and behavior of the floating structure is considered using a numerical analysis. Through reviewed structural performance of a floating structure by live load distribution, the structure presented tensile behavior by two live load cases (A, B, D-type). Then, the other live load cases (C, E, F, G, H, I, J-type) shows compressive behavior. Especially, immoderately compressive stress was generated on bottom slab at specific load distribution. but, that should be decreased through controling buoyancy pre-flexion. Through reviewed structural behavior, slopes of structure by four live load cases (B, E, F, H-type) were exceeded in design criteria of mega-float. It should be estimated that it get out of the load case at loading container. In all, the present study can be considered as a benchmark of a floating container terminal in the absence of analysis and will be used to guide-line about serviceability of concrete floating container terminal.

• Korean Journal of Materials Research
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• v.2 no.4
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• pp.306-310
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• 1992

Effects of hafnium, boron and zirconium on the ductility of Ni$_3$(Al, Fe) intermetallic compounds were studied using tensile test and SIMS analysis. Ni$_3$(Al, Fe) alloy with 0.1 at.% Hf, 0.05 at.% B and 0.1 at.% Zr additions showed maximum elongations of about 30% at 300K, 10% at 300K and 14% at 473K, respectively. The fracture mode of the alloy without the additive was the mixture of intergranular and transgranular fractures, but the addition of Hf, Zr or B changed the fracture mode to transgranular only. SIMS analysis showed that the beneficial effects of Hf, Zr or B segregation on the grain boundary strength are consistent with the grain boundary cohesion theory.

• Phonetics and Speech Sciences
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• v.6 no.3
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• pp.63-72
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• 2014

The current study assessed the utility of acoustic analyses the most commonly used in routine clinical voice assessment including perturbation, nonlinear dynamic analysis, and Spectral/Cepstrum analysis based on signal typing of dysphonic voices and investigated their applicability of clinical acoustic analysis methods. A total of 70 dysphonic voice samples were classified with signal typing using narrowband spectrogram. Traditional parameters of %jitter, %shimmer, and signal-to-noise ratio were calculated for the signals using TF32 and correlation dimension(D2) of nonlinear dynamic parameter and spectral/cepstral measures including mean CPP, CPP_sd, CPPf0, CPPf0_sd, L/H ratio, and L/H ratio_sd were also calculated with ADSV(Analysis of Dysphonia in Speech and VoiceTM). Auditory perceptual analysis was performed by two blinded speech-language pathologists with GRBAS. The results showed that nearly periodic Type 1 signals were all functional dysphonia and Type 4 signals were comprised of neurogenic and organic voice disorders. Only Type 1 voice signals were reliable for perturbation analysis in this study. Significant signal typing-related differences were found in all acoustic and auditory-perceptual measures. SNR, CPP, L/H ratio values for Type 4 were significantly lower than those of other voice signals and significant higher %jitter, %shimmer were observed in Type 4 voice signals(p<.001). Additionally, with increase of signal type, D2 values significantly increased and more complex and nonlinear patterns were represented. Nevertheless, voice signals with highly noise component associated with breathiness were not able to obtain D2. In particular, CPP, was highly sensitive with voice quality 'G', 'R', 'B' than any other acoustic measures. Thus, Spectral and cepstral analyses may be applied for more severe dysphonic voices such as Type 4 signals and CPP can be more accurate and predictive acoustic marker in measuring voice quality and severity in dysphonia.

• Magazine of the Korean Society of Agricultural Engineers
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• v.31 no.1
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• pp.82-95
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• 1989

This study was carried out for the stability analysis of earth dam by the variation of compaction density. The test samples were taken from five kinds of soil used for banking material and the degree of compaction for this samples were chosen 100, 95, 90, 85, and 80 percent. The stability problems were analysed by the settlement and camber( extra banking) of dam, strength parameter and dam slope, and coefficient of permeability and seapage flow through dam body. The results of the stability analysis of earth dam are as follows. 1. The more the fine particle increases and lower the compaction degree becomes, the lower the preconsolidation load becomes but the compression index becomes higher. 2. Sixty to eighty percent of settlement of dam occurs during the construction period and the settlement ratio after completion of dam is inversly proportional to the degree of compaction. 3. The camber of dam has heigher value in condition that it has more fine particle(N) and heigher dam height(H) with the relation of H= e(aN-bH-e). 4. The cohesion(C) decreases in proportion to compaction degree(D) and fine particle(N) with the relation of C= aD+ bN-c, but the internal friction angle is almost constant regardless of change of degree of compaction. 5. In fine soil, strength parameter from triaxial compression test is smaller than that from direct shear test but, they are almost same in coarse soil regardless of the test method. 6. The safety factor of the dam slope generally decreases in proportion to cohesion and degree of compaction but, in case of coarse soil, it is less related to the degree of compaction and is mainly afected by internal friction angle. 7. Soil permeability(K) decreases by the increases of the degree of compaction and fine particle with relation of K=e(a-bl)-cN) 8. The more compaction thickness is, the less vertical permeability (Kv) is but the more h6rzontal permeability (KH) is, and ratio of Kv versus KH is largest in range from 85 to 90 percent of degree of corn paction. 9. With the compaction more than 85 percent and coefficient of permeability less than ${\alpha}$X 10-$^3$cm/sec, the earth dam is generally safe from the piping action.

• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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• 2002.11a
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• pp.187-190
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• 2002

The Electrodeless lamp is intended as a high efficacy replacement for the incandescent reflector lamp in many applications. but Increasing awareness of electromagnetic compatibility(EMC) issues in lighting application, the problems of interference generation by electrodeless fluorescent lighting system have been highlighted. In this paper, Maxwell 2D finite element analysis program(Ansoft) is used to obtain electromagnetic properties associated with the coil and nearby structures. and also evaluated Flux B, H by changing the input current.