• Korean Journal of Remote Sensing
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• v.28 no.2
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• pp.191-202
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• 2012

A high resolution satellite imagery such as KOMPSAT-2 includes a material containing rational polynomial coefficient (RPC) for three-dimensional geopositioning. However, image geometries which are calculated from the RPC must have inevitable systematic errors. Thus, it is necessary to correct systematic errors of the RPC using several ground control points (GCPs). In this paper, we propose an efficient method for automatic correction of image geometries using tie points of a stereo pair and the Shuttle Radar Topography Mission (SRTM) Digital Elevation Model (DEM) without GCPs. This method includes four steps: 1) tie points extraction, 2) determination of the ground coordinates of the tie points, 3) refinement of the ground coordinates using SRTM DEM, and 4) RPC adjustment model parameter estimation. We validates the performance of the proposed method using KOMPSAT-2 stereo pair. The root mean square errors (RMSE) achieved from check points (CPs) were about 3.55 m, 9.70 m and 3.58 m in X, Y;and Z directions. This means that we can automatically correct the systematic error of RPC using SRTM DEM.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.26 no.3D
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• pp.375-383
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• 2006

Nationals Assembly have experiencing unbalance between capacities and demand of parking facilities because of increasing parking demand, free parking policy, long term parking and passenger car use of workers, usage of neighborhood office workers and visitors. The lack of parking facilities cause illegal parking on the street inside and outside of National Assembly which result in the disorder of parking culture. To solve these parking problems, this study suggest parking management system to enter the National Assembly for the short term and charging parking fee, operation by private company, constructing parking building by abrogation of upper limit and construction of ground park, and so on. The study, also, suggested to strengthen weekday or 10 day shift system, to increase shuttle bus and commuting bus, to activation of Han River waterside parking area, public transportation with those new parking management system. For the conclusion, the parking problems of National Assembly should be focused more on parking management than the parking facilities supply.

• Journal of the Korean Association of Geographic Information Studies
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• v.21 no.2
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• pp.34-43
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• 2018

DEM(Digital Elevation Model) being fundamental data in geospatial domain is produced from different data and using various methods. As they are derived their precision varies and altering derivative in application. In this work, we compare two widely used DEMs namely(Advanced Land Observing Satellite World 3D 30m and Shuttle Radar Topography Mission Global 30m) around coastal islands in Gyeongsangnam-do, South Korea. First statistics of each DEM were calculated and later overlapped in Landsat image for visual comparison of areas below and above zero-meter elevation. As a result, it was found that DEMs were highly correlated with each other and had similar statistics. Besides having few high differences in hilly land area, they were able to represent the coastal lines. It has also been noted that they have many negative values and should carefully select study area covering full watershed in coastal regions to avoid negative elevation even after filling the sinks.

• Journal of Microbiology
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• v.39 no.1
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• pp.31-36
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• 2001

A cDNA clone encoding the ribosomal protein S20 has been isolated from the Schizosaccharomyces pombe cDNA library by colony hybridization. The insert contained in the original plasmid pYJ10 was transferred intro shuttle vector pRS316 generate plasmid pYJll. The dDNA insert of plasmid pYJll, contains 484 nucleotides and encodes a protein of 118 amino acids with a calculated mass of 13,544 daltons. The deduced amino acid sequence of S. pombe ribosomal protein S20 is very homologous with fruit fly, rat, and budding yeast counterparts. It is also homologous with Xenopus S22 ribosomal protein. S. pombe ribosomal protein S20 appears to be relatively hydruphobic except the C-terminal region. The 728 bp upstream region of the S20 gene was amplified from chromosomal DNA and transferred into the BamHI/EcoRI site of the promoterles $\beta$-galactosidase gene of the vector YEp357R, which resulted in fusion plasmid pYS20. The synthesis of $\beta$-galactosidase from the fusion plasmid appeared to be the highest in the mid-exponential phase. The S. pombe cells with the fusion plasmid grown at 35$\^{C}$ gave lower $\beta$-galactosidase activity than the cells grown at 30$\^{C}$. Computer analysis showed the consensus sequence CAGTCACA in the upstream regions of various ribosomal protein genes in S. pombe, which would be involved in the coordinated expression of small ribosomal proteins.

• Environmental Analysis Health and Toxicology
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• v.14 no.1_2
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• pp.1-12
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• 1999

Recently, several new methods for the detection of genetic damages in vitro and in vivo based on molecular biological techniques were introduced according to the rapid progress in toxicology combined with cellular and molecular biology. Among these methods, mouse lymphoma thymidine kanase (tk) gene forward mutation assay, single cell gel electrophoresis (comet assay) and transgenic animal and cell line model as a target gene of lac I (Big Blue) and lac Z (Muta Mouse) gene mutation are newly introduced based on molecular toxicological approaches. The mouse lymphoma tk$\^$+/-/ gene assay (MOLY) using L5178Y tk$\^$+/-/ mouse lymphoma cell line is one of the mammalian forward mutation assays, and has many advantages and more sensitive than hprt assay. The target gene of MOLY is a heterozygous tk$\^$+/-/ gene located in 11 chromosome, so it is able to detect the wide range of genetic changes like point mutation, deletion, rearrangement, and mitotic recombination within tk gene or deletion of entire chromosome 11. The comet assay is a rapid, simple, visual and sensitive technique for measuring and analysing DNA breakages in mammalian cells, Also, transgenic animal and cell line models, which have exogenous DNA incorporated into their genome, carry recoverable shuttle vector containing reporter genes to assess endogenous effects or alteration in specific genes related to disease process, are powerful tools to study the mechanism of mutation in vivo and in vitro, respectively. Also in vivo acridine orange supravital staining micronucleus assay by using mouse peripheral reticulocytes was introduced as an alternative of bone marrow micronucleus assay. In this respect, there was an International workshop on genotoxicity procedure (IWGTP) supported by OECD and EMS (Environmental Mutagen Society) at Washington D. C. in March 25-26, 1999. The objective of IWGTP is to harmonize the testing procedures internationally, and to extend to finalization of OECD guideline, and to the agreement of new guidelines under the International Conference of Harmonization (ICH) for these methods mentioned above. Therefore, we introduce and review the principle, detailed procedure, and application of MOLY, comet assay, transgenic mutagenesis assay and supravital staining micronucleus assay.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2000.10a
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• pp.189-195
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• 2000

New concept of LNG-FPSO ship with moonpool and bilge step in bottom is considered and investigated in the point of motion reduction and sloshing phenomena of the cargo and operation tanks. The cargo capacity of the ship of which principle dimensions is L x B x D x t(design) =270.0 x 51.0 x 32.32 x 13.7(m) 16K at 98% loading condition. The two moonpools and rectangular step at bilge part are setted up specially for getting the effect of motion decrease. For the motion analysis, linearized three dimensional diffraction theory with the simplified boundary conditions is used. The six-degree of freedom coupled motion responses are calculated for the LNG-FPSO ship. Viscous effects on the roll motion responses of a vessel are taken into account in this calculation program using an empirical formula suggested by Ikeda, Himeno and Tanaka is used. The case study for the moonpool size had been carried out by theoretical estimation and experimental method. For the optimization of the moonpool size and effect of the step, 9 cases of its size and with and without step are considered. From the results of calculation and experiment, it can be concluded that this designed LNG-FPSO ship have possibility to carry out her missions in the rough sea as for the owner's demand waves condition. The motion responses, especially roll motion, for the designed LNG-FPSO ship are much lower than those of another drillship and shuttle tanker and limit criterions are satisfied. For the check of the cargo tank and operation tank sizes we have performed sloshing analysis in the irregular waves which focuses on the pressure distribution on the tank wall and the time history of pressure and free surface for No.2 and No5. tanks of LNG-FPSO with chamfers. Finally we got the tank size which has no resonance and no impact pressure in all filling in the bow quartering and beam sea.

• Journal of Ocean Engineering and Technology
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• v.16 no.1
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• pp.1-7
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• 2002

본 논문에서는 선체 하부에 moonpool과 bilge step을 장착한 새로운 개념으의 LNG-FPSO를 운동감소와 cargo, operation tank의 슬로싱 현상의 관점에서 기술하였다. LNG-FPSO의 주요제원은$L\times B\times D\times t(design)=270.0\times51.0\times32.32\times13.7(m)$ 이고 적용조건은 total corgo capacity of 161KT at 98% loading condition 이다. LNG-FPSO의 운동감소의 목적으로 2개의 moonpool과 선체하부 bilge 부분에 사각 step을 장착하였다. LNG-FPSO의 운동해석을 위해 단순화된 경계조건을 만족하는 선형화된 3차원 diffraction theory를 사용하였고 LNG-FPSO의 연성된 6-자유도 운동응답을 계산하였다. LNG-FPSO의 정확한 Roll 운동을 추정하기 위해 점성효과는 Himeno(1981)가 제안한 경험식을 사용하였다. Moonpool의 크기에 따른 운동감소의 경향을 파악하기 위해 이론적 계산과 실험적 방법으로 수행하였다. Moonpool 크기와 bilge step의 효과를 최적화하기 위해 총9가지의 case를 설정하였다. 이론 및 실험 결과로부터 본 LNG-FPSO는 moonpool과 bilge step의 장착으로 인한 감쇠력의 증가로 운동성능이 우수하다. 본 LNG-FPSO의 운동 응답중, 특별히 roll 운동이 다른 drillship, shuttle tanker등의 선박과 비교하여 상당히 작았고 이는 moonpool과 blige step의 장착으로 인한 효과로 판단된다. Cargo tank와 operation tank 크기를 검토 하기 위해 불규칙 해상중 sloshing 해석을 chamfer를 갖는 LNG-FPSO의 No.2, No.5 tank 벽면의 압력 분포와 자유표면의 time history에 초점을 맞추어 수행하였다. 최종적으로 tank 크기를 최적화 하였고 최적화된 tank는 선수사파와 횡파상태의 모든 filling에서 공진현상과 충격압력이 발생하지 않음을 확인하였다.

• Journal of the Korean Society for Heat Treatment
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• v.6 no.4
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• pp.237-242
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• 1993

The fatigue behaviors of mechanically alloyed Al-4Mg alloys dispersed with either $Al_2O_3$ or $MgAl_2O_4$ oxide particles were investigated. This study maily concerned with the role of coherency of dispersed particles with the matrix on the fatigue behavior of the alloys. The $MgAl_2O_4$ which has a spinel structure with the lattice parameter of exactly the twice of Al showed the habit relation with the matrix. The mechanically alloyed Al-4Mg alloys showed stable stress responses with fatigue cycles from start to failure regadless of strain amplitudes and of existence of dispersoids. The Al-4Mg alloy dispersed with $MgAl_2O_4$ showed not only the better static mechanical properties but also the better low cycle fatigue resistance than that with $Al_2O_3$, i.e., much higher plastic strain energy dissipated to failure, at low strain amplitude. However, this alloy showed inferior fatigue resistance to that dispersed with $Al_2O_3$ or that without dispersion at high strain amplitude. These results imply that $MgAl_2O_4$ may promote lowering the stacking fault energy of the alloy inherited from the coherency with the matrix so that dislocations shuttle back and forth on the same slip plane without cross slipping to other planes during fatigue at low strain amplitude resulting in long fatigue life.

• Journal of Microbiology and Biotechnology
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• v.11 no.3
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• pp.508-514
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• 2001

A stuructural gene (ycl) encoding novel yeast cell wall hydrolase, YCL, was cloned from alkalophilic Bacillus alcalophilus subsp. YB380 by PCR, and transformed into E. coli JM83. Based on the N-terminal and internal amino acid sequences of the enzyme, primers were designed for PCr. The positive clone that harbors 1.8 kb of the yeast cell wall hydrolase gene was selected by the colony hybridization method with a PCR fragment as a probe. According to the computer analysis, this gene contained a 400-base-paired N-terminal domain of the enzyme. Based on nucletide homology of the cloned gene, a 850 bp fragment was amplified and the C-terminal domain of the enzyme was sequenced. With a combination of the two sequences, a full nucleotide sequence for YCL was obtained. This gene, ycl, consisted of 1,297 nucleotides with 27 nucleotides with 27 amino acids of signal sequence, 83 redundant amino acids of prosequence, and 265 amino acids of the mature protein. This gene was then cloned into the pJH27 shuttle vector and transformed into the Bacillus subtilis DB104 to express the enzyme. It was confirmed that the expressed cell wall hydrolase that was produced by Bacillus subtilis DB104 was the same as that of the donor strain, by Western blot using polyclonal antibody (IgY) prepared from White Leghorn hen. Purified yeast cell wall hydrolase and expressed recombinant protein showed a single band at the same position in the Western blot analysis.

• International Journal of Ocean Engineering and Technology Speciallssue:Selected Papers
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• v.5 no.1
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• pp.22-28
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• 2002

A New concept for the LNG-FPSO ship, with moonpool and bilge step in bottom, is proposed. This concept is investigated with regard to motion reduction and sloshing phenomena of the cargo and operation tanks. The principal dimensions of the ship are $L\timesb B\times D\times t(design)=270.0\times51.0\times32.32\times13.7(m)$, with a total cargo capacity of 161KT; a 98% loading condition is considered for this study. The moonpools and rectangular step at the bilge have been designed for the purpose of decreasing the motion within the tank. For the motion analysis, linearized three-dimensional diffraction theory, with the simplified boundary condition was used. The six-degree of freedom coupled motion responses were calculated for the LNG-FPSO ship. Viscous effects on the roll motion responses of a vessel were taken into account in this calculation program, using an empirical formula suggested by Himeno(1981). The case study for the moonpool size has been conducted using theoretical estimation and the experimental method. For the optimization of the moonpool size and effect of the bilge step, 9 cases of its size, both with and without bilge step, were involved in the study. no motion responses, especially roll motion, for the designed LNG-FPSO ships are much lower than those of other drill ships and shuttle tankers. The limit criterions are satisfied. To check the cargo tank and operation tank sizes, we performed a sloshing analysis in the irregular waves which focuses on the pressure distribution on the tank wall and the time history of pressure and free surface for No.2 and 5 tanks of LNG-FPSO with chamfers. Finally, optimum tank sire was estimated.