• Journal of Microbiology and Biotechnology
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• 제30권2호
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• pp.155-162
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• 2020

Acetyl xylan esterase (AXE; E.C. 3.1.1.72) is one of the accessory enzymes for xylan degradation, which can remove the terminal acetate residues from xylan polymers. In this study, two genes encoding putative AXEs (LaAXE and BhAXE) were cloned from Lactobacillus antri DSM 16041 and Bacillus halodurans C-125, and constitutively expressed in Escherichia coli. They possess considerable activities towards various substrates such as p-nitrophenyl acetate, 4-methylumbelliferyl acetate, glucose pentaacetate, and 7-amino cephalosporanic acid. LaAXE and BhAXE showed the highest activities at pH 7.0 and 8.0 at 50℃, respectively. These enzymes are AXE members of carbohydrate esterase (CE) family 7 with the cephalosporine-C deacetylase activity for the production of antibiotics precursors. The simultaneous treatment of LaAXE with Thermotoga neapolitana β-xylanase showed 1.44-fold higher synergistic degradation of beechwood xylan than the single treatment of xylanase, whereas BhAXE showed no significant synergism. It was suggested that LaAXE can deacetylate beechwood xylan and enhance the successive accessibility of xylanase towards the resulting substrates. The novel LaAXE originated from a lactic acid bacterium will be utilized for the enzymatic production of D-xylose and xylooligosaccharides.

• Asian-Australasian Journal of Animal Sciences
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• 제28권7호
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• pp.999-1005
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• 2015

Bacillus-based feed additive was evaluated for its efficacy on growth performance, nutrient digestibility, fecal gas emission, and the consumption of time and amount of water for cleaning the pen of growing finishing pigs. A total of 120 growing pigs ($23.59{\pm}1.41kg$) were used in a 16-wk feeding trial. Pigs were randomly distributed into 1 of 2 treatments on the basis of body weight and sex. There were 12 replicate pens per treatment, with 5 pigs (3 barrows and 2 gilts) per pen. Dietary treatments were CON which was basal diet, and T1 which was CON+62.5 ppm microbial feed additive that provided $1.47{\times}10^8cfu$ of Bacillus organisms per gram of supplement. During the weeks 0 to 6, average daily gain (ADG) in T1 treatment was higher (p<0.05) than CON, but no improvement in average daily feed intake (ADFI) and feed efficiency (G:F) was noted. During 6 to 16 weeks, no difference (p>0.05) was noted in growth performance. However, ADG was improved (p<0.05) and overall ADFI tended (p = 0.06) to improve in T1 compared with CON. At week 6, the co-efficient of apparent total tract digestibility (CATTD) of dry matter (DM) nitrogen (N) was increased (p<0.05) in T1 compared with CON. Fecal $NH_3$ emission was decreased (p<0.05) in T1 compared with CON, at the end of 6th and 15th weeks. The time and water consumed for washing the pens were decreased (p<0.05) in T1 compared with CON. In conclusion, supplementation with Bacillus-based feed additive could improve the overall growth performances, increase the CATTD of DM and decrease the fecal $NH_3$ content and the time and water consumed in washing the pens for growing-finishing pigs.

• 생물정신의학
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• 제15권2호
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• pp.92-100
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• 2008

Objectives : The purpose of this study was to investigate 1) the neuropsychological deficits with major depressive disorder(MDD) in depressed state and 2) the changes of neuropsychological dysfunctions during depressed episodes and remitted periods in the MDD group. Methods : 12 patients with MDD and 70 normal controls who were diagnosed and classified by DSM-IV and SCID-IV interview participated in this study. The psychopathology was measured using the Hamilton rating scale for depression(HAM-D) and Brief Psychiatric Rating Scale(BPRS). The memory function, executive function, and sustained attention were measured by a trained psychologist using the Korean version of Memory Assessment Scale(K-MAS), Wisconsin Card Sorting Test(WCST), and Vigilance(VIG) and Cognitrone (COG) in Vienna Test System. After 6 weeks of treatment, we retested the cognitive tests in order to measure the cognitive functions in remitted states. Results : Patients with MDD achieved significantly lower score in sentence immediately recall, verbal memory score and total memory score of the K-MAS, total errors of the WCST, response time of Vigilance and response time at "Yes" response of Cognitrone than normal controls at baseline. After 6 weeks of medication, the psychiatric symptoms in the patient group were attenuated, and most of the neuropsychological functions including attention, memory, and frontal/executive function were improved except for response time of Cognitrone. Conclusions : This study provides evidence for distinct neuropsychological deficits in patients with MDD on their depressed states and remitted periods. The impairment on response time remains after remission, and this would be a trait marker of major depressive disorder.

• Asian-Australasian Journal of Animal Sciences
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• 제31권10호
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• pp.1611-1618
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• 2018

Objective: The objective of this experiment was to examine the effects of different amylose/amylopectin ratios on rumen fermentation and development of fattening lambs. Methods: Forty-eight 7-day-old male Small-tailed Han sheep${\times}$Northeast fine wool sheep were randomly assigned to four treatments of dietary amylose/amylopectin ratios (0.12, 0.23, 0.24, and 0.48 in tapioca starch, corn starch, wheat starch and pea starch diets, respectively). Three lambs from each treatment were slaughtered at 21, 35, 56, and 77 days of age to determine the rumen fermentation and development. Results: Compared with tapioca starch diet, the pea starch diet significantly increased the concentration of ammonia nitrogen in the ruminal fluid of lambs but significantly decreased the bacterial protein content. At 56 and 77 d, the rumen propionate concentration tended to be greatest in the tapioca starch group than in other groups. The rumen butyrate concentration was the greatest in lambs fed on pea starch compared with those fed on other starch diets. Furthermore, the pea starch diet significantly stimulated rumen development by increasing the papillae height, width and surface area in the rumen ventral or dorsal locations in lambs. However, different amylose/amylopectin ratios diets did not significantly affect the feed intake, body weight, average daily gain, the relative weight and capacity of the rumen in lambs with increasing length of trial periods. Conclusion: Lambs early supplemented with a high amylose/amylopectin ratio diet had favourable morphological development of rumen epithelium, which was not conducive to bacterial protein synthesis.

• Journal of Microbiology
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• 제34권2호
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• pp.172-178
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• 1996

Methylobacillus sp. strain SK1, which grows only on methanol, was found to grow in the absence of added copper. The doubling time (t$_{d}$ = 1.3 h) of the bacterium growing at the exponential growth phase at 30.deg.C in the absence of copper was the same as that of the cell growing in the presence of copper. The bacterium growing after the exponential phase in the absence of copper, however, grew faster than the cell growing in the presence of copper. Cells harvested after thee arly stationary phase in the presence of copper were found to exhibit no methanol dehydrogenase (MDH) activity, but the amount and subunit structure of the enzyme in the cells were almost the same as that in cells harboring active MDH. Pellets of the cells harvested after the early stationary phase in the presence of copper were pale green. Cell-free extracts prepared from cells harvested at the early stationary phase in the presence of copper were pink and exhibited MDH activity, but it turned dark-green rapidly from the surface under air. The green-colored portions of the extracts showed no MDH activity and contained c-type cytochromes that were oxidized completely. The inactive MDH activity and contained c-type cytochromes that were oxidized completely. The inactive MDH proteins in the green portions were found to have antigenic sites identical to those of the active one as the inactive MDHs in cells grown in the presence of copper. The bacterium was found to accumulate copper actively during the exponential growth phase. MDH prepared from cells grown in the presence or absence of copper was found to be more stable under nitrogen gas than under air. Methanol at 10 mM was found to enhance the stability of the MDH under air.r.

• 한국식품과학회지
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• 제43권3호
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• pp.369-374
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• 2011

D. geothermalis의 dgeo_0475 유전자로부터 만들어지는 효소를 정제하여 그 특성을 확인하였다. DGMA는 분자량이 약 68 kDa 크기의 효소로서 ${\beta}$-CD, soluble starch 및 pullulan을 가수분해하는 CD 분해 효소임을 확인하였다. 효소의 최적 온도는 $40^{\circ}C$ 최적 pH 는 6.0이며 대부분의 기질들을 glucose와 maltose로 가수분해 하였고 pullulan 및 soluble starch로부터 미량의 panose를 생성하였다. ${\beta}$-CD를 가장 잘 가수분해하나 기질간 상대적 활성차이는 다른 CD 분해효소에 비하여 크지 않았다.

• Journal of Microbiology and Biotechnology
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• 제32권7호
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• pp.855-861
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• 2022

A white-pigmented, non-motile, gram-negative, and rod-shaped bacterium, designated CYS-02^T, was isolated from soil sampled at Suwon, Gyeonggi-do, Republic of Korea. Cells were strictly aerobic, grew optimally at 20-28℃ and hydrolyzed Tween 40. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain CYS-02^T formed a lineage within the family Comamonadaceae and clustered as members of the genus Variovorax. The closest members were Variovorax guangxiensis DSM 27352^T (98.6% sequence similarity), Variovorax paradoxus NBRC 15149^T (98.5%), and Variovorax gossypii JM-310^T (98.3%). The principal respiratory quinone was Q-8 and the major polar lipids contain phosphatidylethanolamine (PE), phosphatidylethanolamine (PG), and diphosphatidylglycerol (DPG). The predominant cellular fatty acids were C_16:0, summed feature 3 (C_16:1ω7c and/or C_16:1ω6c) and summed feature 8 (C_18:1ω7c and/or C_18:1ω6c). The DNA GC content was 67.7 mol%. The ANI and dDDH values between strain CYS-02T and the closest members in the genus Variovorax were ≤ 79.0 and 22.4%, respectively, and the AAI and POCP values between CYS-02^T and the other related species in the family Comamonadaceae were > 70% and > 50%, respectively. The genome of strain CYS-02^T showed a putative terpene biosynthetic cluster responsible for antioxidant activity which was supported by DPPH radical scavenging activity test. Based on genomic, phenotypic and chemotaxonomic analyses, strain CYS-02^T was classified into a novel species in the genus Variovorax, for which the name Variovorax terrae sp. nov., has been proposed. The type strain is CYS-02^T (= KACC 22656^T = NBRC 00115645^T).

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2022년도 학술발표회
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• pp.445-445
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• 2022

최근 새만금 방조제 건설이 완료됨에 따라 주변 연안지역의 지형에 많은 변화가 감지되었다. 본 연구대상지는 격포해수욕장으로서 새만금 사업 준공 후 연안침식에 따른 모래 유실 등으로 인해 양빈사업 등이 검토되고 있는 상황이다. 본 연구에서는 연안지형 변화 탐지를 위한 UAV (Unmanned Aerial Vehicle) 활용기술을 제시하는 것으로서 총 3회에 걸쳐 UAV 영상을 촬영하였다. 영상촬영은 DJI Inspire 2 UAV를 활용하였으며 VRS(Virtual Reference Service) 측량성과와 연계하여 Pix4D Mapper SW를 통해 정사영상과 수치표면모델(DSM; Digital Surface Model)을 제작하였다. 먼저 2018. 6. 29 ~ 2018. 12. 10 사이의 지형변화 탐지를 수행한 결과 침식과 퇴적의 최대값은 각각 2.56m와 2.24m로 나타났으며 평균적으로는 0.01m의 퇴적이 발생하였다. 그리고 2018. 6. 29 ~ 2019. 6. 14 동안의 침식과 퇴적의 최대값은 각각 2.31m와 2.28m로 나타났으며 평균값은 0.02m의 침식이 발생하였다. 또한 2018. 12. 10 ~ 2019. 6. 14 사이에는 침식과 퇴적의 최대값이 각각 2.28m와 2.55m로 나타났으며 평균값은 0.03m의 침식이 발생하였다. 지형변화를 보다 상세히 모니터링하고자 퇴적과 침식구간을 나누어 분석을 수행한 결과, 2018. 6. 29 ~ 2018. 12. 10 사이에는 0.5m 이내의 침식과 퇴적구간 면적이 각각 13,324.4m²와 14,667.3m²로 퇴적구간의 면적이 1,342.9m² 만큼 높게 나타났으며, 2018. 12. 10 ~ 2019. 6. 14 사이에는 0.5m 이내의 침식과 퇴적구간 면적이 각각 16,176.6m²와 11,723.0m²로 침식구간의 면적이 4,453m² 만큼 높게 나타났다. 또한 2018. 12. 10 ~ 2019. 6. 14 사이에는 0.5m 이내의 침식과 퇴적구간 면적이 각각 16,821.6m²와 11,126.4m²로 침식구간의 면적이 5,695.2m² 만큼 크게 분석되었다. 이와 같이 UAV 영상 기반의 연안지형 모니터링을 수행할 경우 시계열 지형변화를 효과적으로 모니터링할 수 있으며, 이러한 업무는 새만금 방조제 건설에 따른 지형변화의 영향평가 등 다양한 연안업무에 활용될 수 있을 것이다.

• 생물정신의학
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• 제3권2호
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• pp.277-287
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• 1996

본 연구는 주정중독에 동반된 우울증 환자의 치료에서 적어도 행동학적 부작용의 관점에서는 PAR이 AMI보다 우수함을 증명하였다. 이러한 PAR의 행동학적 효과의 우수성은 환자의 삶의 질을 향상시키고 결국 치료결과를 높이는데 관여하리라고 생각된다.

• 한국지리정보학회지
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• 제14권1호
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• pp.84-93
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• 2011

제방선 지도화는 하천지역의 환경보호와 하천 범람 방지, 그리고 하천 개발에 있어 매우 중요하다. 라이다(LiDAR)와 항공 정사영상(aerial ortho-image)과 같은 원격탐사 데이터의 활용은 대상 지역에 접근하지 않고도 대상 지역에 관한 지형 정보를 얻을 수 있다는 점 때문에, 하천 지도화 작업에 효율적이다. 라이다 자료는 얕은 물을 관통하는 능력과 높은 수직 정확도 때문에 하천구역 지도화 작업에 활용되어 오고 있다. 영상자료의 활용 또한 영상처리 기법을 이용하여 여러 특징들을 추출할 수 있다는 점 때문에 하천 지도화 작업에 효율적이다. 본 논문에서는 라이다와 항공 정사영상을 각각 활용하여 3차원 제방선 지도화 작업을 수행하였다. 그리고 지상 실측정보들을 통해 두 자료로부터 추출된 제방선들의 정확도를 측정하고, 두 측정 결과들을 비교한다. 통계적인 결과에서 나타나듯이 라이다를 활용하여 추출된 3차원 제방선이 항공 정사영상을 활용하여 추출된 3차원 제방선에 비해 수평 및 수직 정확도가 훨씬 더 높다는 것을 보여준다.