Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Effect of Copper on the Growth and Methanol Dehydrogenase Activity of Methylobacillus sp. Strain SK1 DSM 8269

  • Kim, Si W. (Dept. of Biology, Yonsei Univ.) ;
  • Kim, Young M. (Dept. of Biology, Chosun University)
  • Published : 1996.06.01
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Abstract

Methylobacillus sp. strain SK1, which grows only on methanol, was found to grow in the absence of added copper. The doubling time (t$_{d}$ = 1.3 h) of the bacterium growing at the exponential growth phase at 30.deg.C in the absence of copper was the same as that of the cell growing in the presence of copper. The bacterium growing after the exponential phase in the absence of copper, however, grew faster than the cell growing in the presence of copper. Cells harvested after thee arly stationary phase in the presence of copper were found to exhibit no methanol dehydrogenase (MDH) activity, but the amount and subunit structure of the enzyme in the cells were almost the same as that in cells harboring active MDH. Pellets of the cells harvested after the early stationary phase in the presence of copper were pale green. Cell-free extracts prepared from cells harvested at the early stationary phase in the presence of copper were pink and exhibited MDH activity, but it turned dark-green rapidly from the surface under air. The green-colored portions of the extracts showed no MDH activity and contained c-type cytochromes that were oxidized completely. The inactive MDH activity and contained c-type cytochromes that were oxidized completely. The inactive MDH proteins in the green portions were found to have antigenic sites identical to those of the active one as the inactive MDHs in cells grown in the presence of copper. The bacterium was found to accumulate copper actively during the exponential growth phase. MDH prepared from cells grown in the presence or absence of copper was found to be more stable under nitrogen gas than under air. Methanol at 10 mM was found to enhance the stability of the MDH under air.r.

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References

  1. The biochemistry of methlotrophs Anthony, C
  2. Adv. Microbial Physiol. v.27 Bacterial oxidation of methane and methanol Anthony, C
  3. Bacterial energy transduction Quinoproteins and energy transduction Anthony, C.;C. Anthony(ed.)
  4. Biochem. J. v.104 The microbial oxiation of methanol: Prurification and some properties of the alcohol dehydrogenase of Pseudomonas sp. M27 Anthony, C.;L.Z. Zatman
  5. J. Gen. Microbiol. v.135 The role o cytochromes and blue copper proteins in growth of an obligate methlotroph on methanol and methylamine Auton, K.A.;C. Anthony
  6. Biochem. J. v.260 The methylamine oxidase system of an obligate methylotroph Auton, K.A.;C. Anthony
  7. Methane and methanol utilizers The physiology and biochemistry of aerobic methanol-utilizing Gram-negative and Gram-positive bacteria Dijkhuizen, L.;P.R. Levering;G.E. de Vries;J.C. Murrell(ed.);H. Dalton(ed.)
  8. Standard methods for the examination of water and wastewater(16th ed.) Determination of metals Greenberg, A.E.;R.R. Trussell;L.S. Clesceri;M.A.H. Franson
  9. J. Bacteriol. v.171 Purification and some properties of carbon monxide dehydrogenase from Acinetobacter sp. strain JC1 DSM 3803 Kim, K.S.;Y.T. Ro;Y.M. Kim
  10. Kor. J. Micrboiol. v.29 Methanol dehydrogenase of an obligate methanolotroph Kim, S.W.;B.H. Kim;Y.M. Kim
  11. Mol. Cells v.1 Methanol dehydrogenase of an obligate methanolotroph Kim, S.W.;Y.T. Ro;Y.M. Kim
  12. J. Bacteriol. v.148 Purification and some properties of carbon monoxide dehydrogenase from Pseudomonas carboxydohydrogena Kim, Y.M.;G.D. Hegeman
  13. FEMS Microbiol. Lett. v.29 Relationship between carbon monoxide dehydrogenase and a small plasmid in Pseudomonas carboxydovorans Kwon, M.O.;Y.M. Kim
  14. Nature v.227 Cleavage of sturctural proteins during the assmelby of the head of phage T Laemmli, U.K
  15. J. Gen. Microbiol. v.131 The role of cytochromes and blue copper proteins in the oxidation of methanol and methylamine in organism 4025, an obliagate methylotroph Lawton, S.A.;C. Anthony
  16. Annu. Rev. Mcirobiol. v.44 Methylotrophs: Genetics and commerical applications Lidstrom, M.E.;D.I. Stirling
  17. J. Biol. Chem. v.193 Protein measurment with the Folin phenol reagent Lowry, O.H.;N.J. rosebrough;A.L. Farr;R.J. rANDALL
  18. Handbook of experimental immunology Immunodiffusion and immunoelectrophoresis Ouchterlony, O.;L.A. Nilsson
  19. Anal. Biochem. v.118 A new discontinuous buffer system for the electrophoresis of cationic proteins at enar-neutral pH Thomas, J.M.;M.E. Hodes
  20. Biotechnol. Lett. v.6 Copper requirement and its influences on the growth of the obligate methylotrophic bacterial strain 4025 Vrdoljak, M.;C. Marie;V. Johanides
  21. J. Biol. Chem. v.244 The reliability of molecular weight determination by dodecyl sulfate-polyacrylamide gel electrophoresis Weber, K.;M. Osborn