• Asian-Australasian Journal of Animal Sciences
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• v.23 no.12
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• pp.1543-1551
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• 2010

A major aim of cattle genome research is to identify candidate genes associated with meat quantity and quality through QTL analysis for application in the livestock industry. Therefore, this study focused on discovery of useful SNPs within the LOC534614 gene, containing 12273_165 SNP which is located on the same site as the QTL on chromosome 6, and evaluation of the association between SNP and body weight and cold carcass weight in Hanwoo (Korean cattle) As a result of a BLAST search of the NCBI web site, we discovered that the mRNA sequence of the LOC534614 gene was similar to that of the coiled-coil domain containing 158 (CCDC158) for dog and human. According to the direct DNA sequence from the CCDC158 gene, we identified 19 polymorphic SNPs within exons and their flanking regions. Among them, 17 polymorphic SNPs were selected for genotyping in Hanwoo (n = 476) and seventeen marker haplotypes containing 12273_165 SNP (frequency >0.1) were identified. As a result of the association between 17 polymorphic SNPs and Hanwoo (n = 476), g.8778G>A SNP in exon 6 was found to be a non-synonymous SNP, and was significantly associated with body weight and cold carcass weight (p<0.05). We discovered 19 polymorphic SNPs in the CCDC158 gene on the QTL region of BTA 6 in Hanwoo and identified that the g.8778G>A SNP was significantly associated with body weight and cold carcass weight (p<0.05), which causes an amino acid variation from valine to methionine. Furthermore, statistical analysis demonstrated that the CCDC158 gene is strongly associated with body weight and cold carcass weight in Hanwoo. In this regard, the g.8778G>A SNP in the CCDC158 gene can be useful as a positional candidate for body weight and cold carcass weight for marker-assisted selection in Hanwoo.

• Journal of Korean Tunnelling and Underground Space Association
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• v.16 no.4
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• pp.373-386
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• 2014

This paper concerns the deformation behavior of tunnels crossing weak zone during excavation. A three dimensional finite element model was adopted in order to conduct a parametric study on the orientation of weaj zone in terms of strike and dip angle relative to the tunnel longitudinal axis. The results of the analyses were then analyzed so that the tunnel displacements in terms of the ratios between the crown settlement and springline displacement can be related to the orientation of the weak zone. The results indicate that the displacement ratios between the tunnel crown and springline tend to quantitatively change when a weak zone exists near or ahead of the tunnel suggesting that the displacement ratios can be effectively used to predict the weak zone during tunnelling. Practical implications of the findings are discussed.

• Tunnel and Underground Space
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• v.4 no.2
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• pp.132-143
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• 1994

Many methods and techniques to reduce ground vibrations are well known. Some of them are to adopt electric millisecond detonators with a sequential blasting machine or an initiating system with an adequate number of delay intervals. The types of electric detonators manufactured in korea include instantaneous, decisecond and millisecond delays but numbers of delay intervals are only limited from No.1 to No.20 respectively. It is not sufficient to control accurately millisecond time with these detonators in tunnel excavation. Sequential fire time refers to adding an external time delay to a detonators norminal firing time to obtain sequential initiation and it is determined by sequential timer setting. To reduce the vibration level, sequential blasting machine(S.B.M) with decisecond detonators was adopted. A total of 134 blasts was recorede at various sites. Blast-to-structure distances ranged from 20.3 to 42.0 meter, where charge weight varied from 0.24 to 0.75 kg per delay. The results can be summarized as follow: 1. The effects of sequential blasting machine on the vibration level are discussed. The vibration level by S.B.M are decreased approximately 14.38~18.05% compare to level of conventional blasting and cycle time per round can be saved. 2. The empirical equations of particle velocity were obtained in S.B.M and conventional blasting. V=K(D/W1/3)-n, where the values for n and k are estimated to be 1.665 to 1.710 and 93.59 to 137 respectively. 3. The growth of cracks due to vibrations are found but the level fall to within allowable value.

• Tunnel and Underground Space
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• v.32 no.4
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• pp.272-284
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• 2022

This study conducted tunnel blasting to evaluate the blasting effect of a shear thickening fluid-based blasting stemming material and a sealed plug device under development. STF single stemming and STF stemming materials were combined with plugs to a tunnel blasting to which the SAV-Cut method was applied, and the advanced rate and fragmentation of tunnel blasting muck pile were compared when sand stemming was used. Tunnel advanced rate was evaluated using a 3D laser scanner. When the STF stemming material and STF stemming material with the plug were compared to the sand stemming material, it increased by 5.7 and 5.36%, respectively. As a result of evaluation of the fragmentation of tunnel blasting muck pile, it was the best when the STF stemming material was applied, and it decreased by about 61% compared to the case of sand stemming blasting. However, no significant improvement in blasting effect was observed with the application of plug devices.

• Journal of Microbiology and Biotechnology
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• v.33 no.11
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• pp.1428-1436
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• 2023

The three Gram-negative, catalase- and oxidase-positive bacterial strains RS43^T, HBC28, and HBC61^T, were isolated from fresh water and subjected to a polyphasic study. Comparison of 16S rRNA gene sequence initially indicated that strains RS43^T, HBC28, and HBC61^T were closely related to species of genus Curvibacter and shared the highest sequence similarity of 98.14%, 98.21%, and 98.76%, respectively, with Curvibacter gracilis 7-1^T. Phylogenetic analysis based on genome sequences placed all strains within the genus Curvibacter. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the three strains and related type strains supported their recognition as two novel genospecies in the genus Curvibacter. Comparative genomic analysis revealed that the genus possessed an open pangenome. Based on KEGG BlastKOALA analyses, Curvibacter species have the potential to metabolize benzoate, phenylacetate, catechol, and salicylate, indicating their potential use in the elimination of these compounds from the water systems. The results of polyphasic characterization indicated that strain RS43^T and HBC61^T represent two novel species, for which the name Curvibacter microcysteis sp. nov. (type strain RS43^T =KCTC 92793^T=LMG 32714^T) and Curvibacter cyanobacteriorum sp. nov. (type strain HBC61^T =KCTC 92794^T=LMG 32713^T) are proposed.

• Geomechanics and Engineering
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• v.36 no.3
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• pp.231-245
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• 2024

In recent decades, the protection and vulnerability of civil structures under explosion loads became a critical issue in terms of security, which may cause loss of lives and structural damage. Concrete retaining walls also restrict soils and slopes from displacements; meanwhile, intensive temporary loading may cause massive damage. In the current study, the modified Johnson-Holmquist (also known as J-H2) material model is implemented for concrete materials to model damages into the ABAQUS through user-subroutines to predict the blasting-induced concrete damages and volume strains. For this purpose, a 3D finite-element model of the concrete retaining wall was conducted in coupled Eulerian-Lagrangian simulation. Subsequently, a blast load equal to 500 kg of TNT was considered in three different positions due to UFC 3-340-02. Influences of the critical parameters in smooth blastings, such as distance from a free face, position, and effective blasting time, on concrete damage rate and destroy patterns, are explored. According to the simulation results, the concrete penetration pattern at the same distance is significantly influenced by the density of the progress environment. The result reveals that the progress of waves and the intensity of damages in free-air blasting is entirely different from those that progress in a dense surrounding atmosphere such as soil. Half-damaged elements in air blasts are more than those of embedded explosions, but dense environments such as soil impose much more pressure in a limited zone and cause more destruction in retaining walls.

• Fisheries and Aquatic Sciences
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• v.27 no.5
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• pp.294-305
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• 2024

Tomini Bay in Gorontalo offers significant potential for amphidromous goby-fry, a group of fish found in at least five estuaries in the area, namely Bone-Bolango, Paguyaman, Bilungala, Tombulilato, and Taludaa Estuaries. Preliminary results were limited to only two locations in estuarine waters, namely the Bone-Bolango and Paguyaman rivers. Further exploration of goby-fry species in various locations within Tomini Bay is essential to enhance knowledge about the abundance and aid conservation efforts. Therefore, this study identified the goby-fry species and created their distribution map in the waters of Tomini Bay Gorontalo. The samples used were obtained from daily catches of fishermen in the five estuaries during the recruitment period from February to September 2022. These samples were categorized based on their morphological similarities and specific melanophore patterns. Distinct groups with different melanophore patterns from those previously reported were identified as newly recorded species, photographed, and described in terms of their morphology. Furthermore, two specimens from each newly recorded species underwent molecular identification using the cytochrome oxidase subunit 1 (COI) gene for DNA amplification and were analyzed through the Basic Local Alignment Search Tool (BLAST) method. The phylogenetic tree was constructed using the Maximum Likelihood Method. The results showed the existence of nongoby-fry species caught together with goby fry school. A total of 75,881 goby-fry and 1,687 nongoby-fry were successfully collected. Among the goby-fry species, 13 were identified, including three new records, namely Eleotris fusca (Forster, 1801), Sicyopterus microcephalus (Bleeker, 1855), and Sicyopus zosterophorus (Bleeker, 1856). This study also documented the existence of nongoby-fry species, namely Anguilla celebesensis (Kaup, 1856), Moringua microchir (Bleeker, 1853), and Microphis leiaspis (Bleeker, 1854). It significantly contributed to the understanding of fish biodiversity in Tomini Bay.

• The Journal of the Acoustical Society of Korea
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• v.37 no.4
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• pp.223-231
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• 2018

Recently, due to various environmental problems, blast tracks in tunnel are replaced with concrete tracks, but they have more adverse effects on noise than blast tracks so that additional noise measures are needed. Among these measures, sound-absorbing blocks start to be used due to its easy and quick installation. However, the performance of sound absorption blocks need to be verified under real environmental and operational conditions. In this paper, interior noise levels in KTX train cruising in Dalseong tunnel are measured before and after the installation of sound-absorbing blocks and the measured data are analyzed and compared. Additionally, noise reduction are estimated by modeling the high speed train, the tunnel and absorption blocks. Measurement devices and methods are used according to ISO 3381 and the equivalent sound pressure levels during the cruising time inside the tunnel are computed. In addition to overall SPLs(Sound Pressure Levels), 1/3-octave-band levels are also analyzed to account for the frequency characteristics of sound absorption and equipment noise in a cabin. In addition, to consider the effects of train cruising speeds and environmental conditions on the measurements, the measured data are corrected by using those measured during the train-passing through the tunnels located before and behind the Dalseong tunnel. Analysis of measured results showed that the maximum noise reduction of 6.8 dB (A) can be achieved for the local region where the sound-absorbing blocks are installed. Finally, through the comparison of predicted 1/3-octave band SPLs for the KTX interior noise with the measurements, the understanding of noise reduction mechanism due to sound-absorbing blocks is enhanced.

• Publications of The Korean Astronomical Society
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• v.12 no.1
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• pp.63-84
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• 1997

We carried out high-resolution(FWHM=3' .3) HI 21 cm observations of the supernova remnant(SNR) PKS0607+17 and HII region S261 using Arecibo 305-m telescope. The observation was to investigate whether the high-velocity(HV) gas detected in the southern area of PKS0607+17 by Koo & Heiles(1991) is physically associated with the SNR or not. The velocity of the HV gas ranges from +64 km/s to +87 km/s, which is difficult to result from the Galactic rotation. The HV gas could be the gas accelerated by supernova blast wave. However, because the observation of Koo and Heiles(1991) was carried out using Hat Creek radio telescope(FWHM $\simeq$ 36'), the association of the HV gas with the SNR could not be investigated. Using the Arecibo HI 21cm data, we have found that the HV gas appears m the southern part of the SNR and its velocity ranges from +61 km/s to +77 km/s. But the HV gas is scattered m the whole field, not only toward PKS0607+17 but also outside the SNR Accordingly the HV gas is probably not associated with the SNR, but is accidentally aligned along the same line of sight toward the SNR. Instead we have found that HI clouds at low velocities could be possibly associated with the SNR. In Arecibo HI 21cm channel maps the HI gas seems to surround the southern boundary of the SNR at $V_{LSR}$ = +19.6 ~ +40.2 km/s. But because the region of the Arecibo HI 21cm observation is not wide enough to examine the HI gas distribution, we investigated this area using the Berkely low-latitude HI survey data(Weaver & Williams 1974) too. There we found HI gas surrounding the radio continuum boundary of PKS0607+17 at $V_{LSR}$ = +21.6 ~ +258 km/s. It is possible that this HI gas is associated with the SNR, in which case, the velocity of the SNR $V_o$ $\simeq$ +26 km/s, its distance d $\simeq$ 12.5 kpc and its radius R $\simeq$ 145 pc. If we assume that the expansion velocity is ~10 km/s, then the age of the SNR is $\sim4.4\times10^6$ years. PKS0607+17 could be one of the oldest SNRs in the Galaxy. We also studied HI propertities of the HII region S261, which is $\sim1^{\circ}$ away from PKS0607+17. There has been no high-resolution m 21 cm observational study on S261. We discovered HI cloud located at the north-eastern part of S261 at $V_{LSR}$ = +5 km/s ~ +10 km/s, which is possibly associated with the HII region. The central velocity of the HI cloud $V_{LSR}$ = +7.2 km/s and the corresponding distance d = 1.5 kpc. This velocity is comparable to the radio recombination line velocities.

• Journal of the Korean Recycled Construction Resources Institute
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• v.6 no.4
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• pp.112-118
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• 2012

Steel industry produces many by-products and wastes such as blast furnace slag, electric arc furnace slag, and converter slag. As in the case of rock, the main component of steel slag are CaO and $SiO_2$ ; further, steel slag is as alkaline as portland cement or concrete. Electric arc furnace oxidizing slag is possible to use as an aggregate for concrete ; however, it has been reclaimed because of it's expansibility caused by free CaO. Recently, a innovative rapid cooling method for melting steel slag has been developed in Korea, which reduces free lime content to minimum level and increases the stability of iron oxide. Therefore, this study describes the results of a series of research to utilize globular shape of electric arc furnace oxidizing slag fine aggregates made by rapidly cooled method for the construction industry by cooling rapidly melted slag from the steel industry. First of all, an experiment was carried out to investigate the quality characteristics of rapidly cooled electric arc furnace oxidizing slag fine aggregates in order to determine whether they can be applied to the construction industry. Then, by applying them to concrete of various particle sizes, we explored experimentally the desired condition to apply rapidly cooled electric arc furnace oxidizing slag fine aggregates to concrete.