• Title/Summary/Keyword: D 002

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Extraction of Total Flavonoids from Lemongrass Using Microwave Energy: Optimization Using CCD-RSM (마이크로웨이브 에너지를 이용한 레몬그라스로부터 플라보노이드 성분의 추출: CCD-RSM을 이용한 최적화)

  • Yoo, Bong-Ho;Jang, Hyun Sick;Lee, Seung Bum
    • Applied Chemistry for Engineering
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    • v.32 no.2
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    • pp.168-173
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    • 2021
  • In this study, we measured total flavonoids after extracting the total flavonoids from lemongrass which is known to have a high content of antioxidant ingredients when using microwave energy. Also, optimal extraction conditions of active ingredients using central composite design-response surface methodology (CCD-RSM) were presented. Both ultrapure water and alcohol were used as extraction solvents and the volume ratio of ethanol/ultrapure water, microwave irradiation time, and microwave irradiation power were set as independence variables. And the extraction yield and total flavonoids were measured. The optimal extraction conditions using CCD-RSM were the volume ratio of ethanol/ultrapure water = 56.3 vol.%, the microwave irradiation time = 6.1 min, and the microwave irradiation power = 574.6 W. We could also obtain expected results of yield = 17.2 wt.% and total flavonoids = 44.7 ㎍ QE/mL dw under the optimum conditions. The comprehensive satisfaction degree of this formula was 0.8562. The P-value was calculated for the yield of 0.037 and the total flavonoids content of 0.002. The average error from actual experiments established for the verification of conclusions was lower than 2.5%. Therefore, a high favorable level could be obtained when the CCD-RSM was applied to the optimization of extraction process.

Effect of Mask Wearing and Type on Cardiopulmonary Resuscitation Accuracy, Fatigue and Physiological Changes

  • Sung-Hwan Bang;Hyo-Suk Song;Gyu-Sik Shim;Hee-Jeong Ahn
    • Journal of the Korea Society of Computer and Information
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    • v.28 no.7
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    • pp.113-120
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    • 2023
  • The purpose of this study was the accuracy of cardiac compression, fatigue, and physiological changes of the rescuer for different mask type in cardiopulmonary resuscitation(CPR). Data collection was from 9 to 12 May 2022, the participants were a total of 24 paramedic students with a BLS provider at D University. The students participated in an experiment in which 12 students each wore a surgical mask (Dental mask) and a fine particle 94% blocking mask (KF94 mask) and performed CPR for 2 minutes over a total of 7 times. As a result of the study, in the analysis of the quality of the rescuer's chest compression according to the type of mask, there was a significant difference in the compression speed (F=24.91, p<.001) and bad compression hand position (F=14.54, p=.024) in the group wearing the KF94, Fatigue showed significant differences in both the Dental mask group (F=51.16, p<.001) and the KF94 mask group (F=63.49, p<.001). Among the physiological changes, heart rate showed a significant difference between the Dental mask group (F=34.79, p<.001) and the KF94 mask group (F=35.55, p<.001), and the respiratory rate showed a significant difference between the Dental mask group (F=25.02, p=.001) and the KF94 mask group(F=23.02, p=.002). Therefore, in order to improve the quality of efficient chest compression and reduce the fatigue and physiological changes of rescuers, it will be necessary for rescuers to wear suitable personal protective equipment.

Proposal of Mobilized Passive Earth Pressure to Allowable Wall Displacement and Movement Types in Sandy Soil (벽체 허용변위와 양상을 고려한 사질토지반에서 수동측토압 제안)

  • Yoon, Young-Ho;Kim, Tae-Hyung;Kim, Tae-O;Woo, Min-seok
    • Journal of the Korean Geotechnical Society
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    • v.39 no.7
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    • pp.5-15
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    • 2023
  • The evaluation of passive earth pressure plays a crucial role in the design of earth-retaining structures such as retaining walls and temporary earth-retaining walls to withstand horizontal earth pressure. In the earth pressure theory, active and passive earth pressures represent the earth pressures at the limit state, where the wall displacement reaches the maximum allowed displacement. In the design of earth-retaining structures, the passive earth pressure is considered as the resisting force. In this context, the limit displacement at which passive earth pressure occurs is significantly greater than that associated with the active earth pressure. Therefore, it is irrational to apply this displacement directly to the calculation of passive earth pressure. Instead, it is necessary to consider the mobilized passive earth pressure exerted at the allowable horizontal displacement to evaluate the structural stability. This study proposes an allowable wall displacement, denoted as 0.002 H (where H represents the excavation depth), based on a literature review that focuses on sandy soils. To calculate the mobilized passive earth pressure from the wall displacement, a semi-empirical equation is proposed. By analyzing the obtained data on mobilized passive earth pressure, a reduction factor applicable to Rankine's passive earth pressure is proposed for practical application in sandy soils under different wall movement types.

Sound Stress Alters Physiological Processes in Digestion and Immunity and Enhances Insecticide Susceptibility of Spodoptera exigua (스트레스 음파에 따른 파밤나방(Spodoptera exigua ) 소화 및 면역 생리작용 저하와 살충제 감수성 제고 효과)

  • Park, Jung-A;Seok, Jung-Kyun;Prasad, Surakasi Venkara;Kim, Yong-Gun
    • Korean journal of applied entomology
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    • v.50 no.1
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    • pp.39-46
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    • 2011
  • This study analyzed effects of different sound treatments in frequencies and intensities on digestion and immune physiological processes of the beet armyworm, Spodoptera exigua larvae. Without effect on egg hatch, sound treatments with 100-5,000 Hz at 95 dB suppressed feeding behavior and inhibited a digestive enzyme activity. In addition, two dimensional electrophoresis of midgut luminal proteins indicated a marked difference of the sound-treated larvae. In response to 5,000 Hz at 95 dB, larvae showed a significant decrease in hemocyte nodule formation against fungal challenge along with significant suppression in phospholipase $A_2$ activity in hemocyte and plasma. With increase of sound frequencies, the treated larvae showed an enhanced susceptibility to insecticides. Such sound frequency effect was significantly modulated with different sound intensities. These results suggest that sound treatment may give adverse stress to physiological processes of S. exigua larvae and may be applied to a nonchemical insect pest control.

Tensile and Fatigue Behavior of ASS304 for Cold Stretching Pressure Vessels at Cryogenic Temperature (Cold Stretching 압력용기용 ASS304 소재의 극저온 인장 및 피로거동)

  • Choi, Hoon Seok;Kim, Jae Hoon;Na, Seong Hyun;Lee, Youn Hyung;Kim, Sung Hun;Kim, Young Kyun;Kim, Ki Dong
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.40 no.5
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    • pp.429-435
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    • 2016
  • Cold stretching(CS) pressure vessels from ASS304 (austenitic stainless steel 304) are used for the transportation and storage of liquefied natural gas(LNG). CS pressure vessels are manufactured by pressurizing the finished vessels to a specific pressure to produce the required stress ${\sigma}_k$. After CS, there is some degree of plastic deformation. Therefore, CS vessels have a higher strength and lighter weight compared to conventional vessels. In this study, we investigate the tensile and fatigue behavior of ASS304 sampled by CS pressure vessels in accordance with the ASME code at cryogenic temperature. From the fatigue test results, we show S-N curves using a statistical method recommended by JSEM-S002. We carried out the fractography of fractured specimens using scanning electron microscopy (SEM).

A Comparative Study on Sleep State, Sleep Time, and Quality of Life of Vietnam Migrant Women by Sasang Constitution (베트남 이주여성의 사상체질별 수면상태, 수면시간 및 삶의 질 비교연구)

  • Jang, Eunsu;Kim, Yunyoung;Baek, Younghwa;Moon, Sujeong;Lee, Siwoo;Kim, Sang-Hyuk
    • Journal of Sasang Constitutional Medicine
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    • v.32 no.1
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    • pp.39-49
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    • 2020
  • The purpose of this study was to find out how sleep characteristics by Sasang constitution (SC) appear in Vietnamese immigrant women in Korea, and how sleep aberration by SC affected the quality of life. The number of 217 Vietnamese immigrant women joined this study. We surveyed SC with Korea Sasang Constitutional Diagnostic Questionnaire (KS-15) and sleep characteristics with Pittsburgh Sleep Quality Index(PSQI) questionnaire. We evaluated quality of life(QoL) with Euro Quality of Life-5Dimension(EQ 5D) questionnaire. A Chi-square test and a one-way ANOVA test were conducted according to SC, and T test also used to compare PSQI groups. Significant p was .05. The number(rate) of Taeeumin(TE), Soeumin(SE), and Soyangin(SY) were 40(18.4%), 55(25.3%), and 122(56.2%) respectively. The distribution of obstructive factors of sleep were significantly different according to SC(Too Cold in Sleeping, x =13.378, p=.037, Too Hot in Sleeping x2=12.834, p=.046). The distribution of daytime drowsiness(x2=15.379, p=.018) and falling concentration power (x2=17.533, p=.008) was significantly different according to SC. The sleep status of the subjects showed a significant negative correlation with the EQ 5D Visual Analogue Scale(VAS) (r =-.399, p<.001), and the EQ 5D Index (r=-.211, p=.002). In the EQ VAS, TE showed a difference between the good and bad sleeping groups (t=3.955, p<.001). SE showed a difference between the two groups in the EQ 5D Index (t=2.332, p=.037). SY did not show a difference between the two groups. This study reveals that sleeping is closely related with QoL and distribution of obstructive factors of sleep may be different according to SC. Further study to confirm the constitutional difference in race at Vietnam is needed.

Association between Dietary Sodium Intake and Abdominal Obesity in Pre-diabetes Korean Adults (전당뇨병 성인에서 나트륨 섭취와 복부비만과의 상관관계)

  • Lim, So Young;Yang, Soo Jin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.5
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    • pp.763-771
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    • 2014
  • The objective of this study was to assess the relationship between dietary sodium intake and prevalence of abdominal obesity in Korean adults. We used data from the Korea National Health and Nutrition Examination Survey V-1 and analyzed data on 4,475 Koreans (${\geq}30$ years old). Subjects were divided into three groups according to fasting plasma glucose (FPG): 1) normal (FPG <100 mg/dL), 2) pre-diabetes ($100mg/dL{\leq}FPG{\leq}125mg/dL$), and 3) diabetes (FPG ${\geq}126mg/dL$ or subjects diagnosed with diabetes). The subjects in each category were stratified by dietary sodium intake as well as index of abdominal obesity. We found that dietary sodium intake was positively correlated with waist circumference (WC) (P=0.002) and was particularly high in the pre-diabetes group. In multiple logistic regression analysis, the normal and diabetes groups showed no association between dietary sodium intake and WC, whereas the pre-diabetes group with a high sodium intake exhibited a significant association (odds ratio (OR)=1.479, P=0.029) between dietary sodium intake and WC. Further, the OR for abdominal obesity in the high sodium intake group with pre-diabetes was 1.590 after adjusting for age and sex (P=0.012). In addition, the ORs for the prevalence of abdominal obesity with fasting glucose, fasting insulin, and homeostasis model assessment of insulin resistance were significantly higher in the pre-diabetes group with high sodium intake compared with low sodium intake. Moreover, these associations were significant even after adjusting for confounding variables (model 2: age and sex; model 3: age, sex, and total energy intake). Our results suggest a strong association between sodium intake and abdominal obesity in pre-diabetes Korean adults.

Interspecific protoplast Fusion and Sexuality in Pleurotus (느타리의 종간(種間) 원형질체(原形質體) 융합(融合) 및 유성(有性))

  • Yoo, Young-Bok;You, Chang-Hyun;Park, Yong-Hwan;Lee, Yeon-Hee;Chang, Kwon-Yawl;Peberdy, John F.
    • The Korean Journal of Mycology
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    • v.15 no.3
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    • pp.135-141
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    • 1987
  • Interspecific heterokaryons were obtained by polyethylene glycol induced fusion of protoplasts from auxotrophic mutants of P. ostreatus+P. cornucopiae, P. ostreatus+P. florida, P. ostreatus+P. sajor-caju, P. florida+P. cornucopiae, P. florida+P. sajor-caju and P. sajor-caju+P. cornucopiae protoplasts. The Fusion products of protoplasts were induced by MCM+benomyl, but segregation of sectors were not identified. Interspecific fusion products of protoplasts between incompatible strains did not form true clamp connections and did not produce fruit bodies. For induction of fertility, interspecific heterokaryons crossed with their parents by hyphal anastomosis.

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Development and Growth of Larvae of Four Bivalve Species (4종 조개류 유생의 발생과 성장)

  • 허영백;허성범
    • Journal of Aquaculture
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    • v.13 no.2
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    • pp.119-128
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    • 2000
  • Exposure to air and increased temperature induced successful spawning in Mytilus edulis, M. coruscus Crassostrea gigas and Pinctata fucata martensii. Developmental durations required for an egg to attain D-shaped larva and the D-shaped larva to reach pediveliger stage were estimated in these bivalves. Size of fertilized eggs was the largest (70.3 ${\mu}m$) in M. coruscus and the smallest (45.3 ${\mu}m$) in P. fucata martensii. At 17$^{\circ}C$, M. edulis and M. coruscus attained D-shaped larval stage within 48 hours after fertilization but those of C. gigas and B. fucata martensiii within 24 and 22 hours at 21 and 26$^{\circ}C$, respectively. The development duration required for a D-shaped larva to attain pediveliger stage was the longest (27 days) in M. coruscus and ranged between 20 and 22 days for the others. The shell length of the pediveliger was the longest (274.9 ${\mu}m$) in C. gigas and smallest (190.9 ${\mu}m$) in P. fucata martensii Length and height of larval shell was highly correlated with each other in all the 4 species. The shell height of C. gigas was more than the shell length beyond the size of 100 ${\mu}m$ shell length. However, shell length of the others was always longer than shell height at the larval stage.

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Response of broilers to supplementation of branched-chain amino acids blends with different valine contents in the starter period under summer conditions

  • Kop-Bozbay, Canan;Akdag, Ahmet;Atan, Helin;Ocak, Nuh
    • Animal Bioscience
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    • v.34 no.2
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    • pp.295-305
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    • 2021
  • Objective: The objectives of this study were to compare the effects of normal and low protein content (PC) of starter diet supplemented or not with blends of branched-chain amino acids (BCAAs) on growth performance of broilers under summer conditions and to investigate whether these effects altered some quality traits and the characteristics of gastrointestinal tract. Methods: A total of 768 mixed-sex broiler chicks (Ross 308, one-d-old) with an average initial body weight (BW) of 47.6±1.03 g were allocated into six treatments with four replications in 2×3 factorial arrangement. Factors were: PC, normal (N, 22% to d 15); and low (L, 20% to d 15); and added BCAA blends, L-leucine, L-isoleucine, and L-valine at zero (0L:0I:0V); 1.0, 0.25, and 0.25 (4L:1I:1V); or 1.0, 0.25, 0.75 (4L:1I:3V) g/kg of diet. Hence, six dietary treatments were named as N0L:0I:0V, N4L:1I:1V, N4L:1I:3V, L0L:0I:0V, L4L:1I:1V, and L4L:1I:3V. Average indoor temperature and humidity were 32.8℃±1.7℃ and 61.1%±4.12%, respectively. Results: BW, feed conversion ratio (FCR) and carcass weight were not affected by PC, BCCA and their interaction (p>0.05). The L diets decreased the water holding capacity of the breast (p = 0.002) and thigh (p = 0.050) meats and dressing percentage (p = 0.005) compared to the N diets. The 4L:1I:1V diet decreased breast yield compared to the 0L:0I:0V diets (p = 0.041). The effect of PC on feed intake, mortality and gastrointestinal trait weight were depended on the L:I:V ratios under summer conditions due to interactions between factors (p<0.05). The FI and mortality of L4L:1I:1V broilers were lower than those of N4L:1I:1V birds (p<0.05). Conclusion: It was concluded that the blends of BCAAs used failed to improve performance and to promote breast yields, because diets with normal or with reduced protein supplemented or not with BCAAs up to d 15 produced a similar BW and FCR in broilers raised in hot-climate conditions.