• Title/Summary/Keyword: Cycle-by-cycle variations

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Development and Validation of Real-time PCR to Determine Branchiostegus japonicus and B. albus Species Based on Mitochondrial DNA (Real-time PCR 분석법을 이용한 옥돔과 옥두어의 종 판별법 개발)

  • Chung, In Young;Seo, Yong Bae;Yang, Ji-Young;Kim, Gun-Do
    • Journal of Life Science
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    • v.27 no.11
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    • pp.1331-1339
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    • 2017
  • DNA barcoding is the identification of a species based on the DNA sequence of a fragment of the cytochrome C oxidase subunit I (COI) gene in the mitochondrial genome. It is widely applied to assist with the sustainable development of fishery-product resources and the protection of fish biodiversity. This study attempted to verify horse-head fish (Branchiostegus japonicus) and fake horse-head fish (Branchiostegus albus) species, which are commonly consumed in Korea. For the validation of the two species, a real-time PCR method was developed based on the species' mitochondrial DNA genome. Inter-species variations in mitochondrial DNA were observed in a bioinformatics analysis of the mitochondrial genomic DNA sequences of the two species. Some highly conserved regions and a few other regions were identified in the mitochondrial COI of the species. In order to test whether variations in the sequences were definitive, primers that targeted the varied regions of COI were designed and applied to amplify the DNA using the real-time PCR system. Threshold-cycle (Ct) range results confirmed that the Ct ranges of the real-time PCR were identical to the expected species of origin. Efficiency, specificity and cross-reactivity assays showed statistically significant differences between the average Ct of B. japonicus DNA ($21.85{\pm}3.599$) and the average Ct of B. albus DNA ($33.49{\pm}1.183$) for confirming B. japonicus. The assays also showed statistically significant differences between the average Ct of B. albus DNA ($22.49{\pm}0.908$) and the average Ct of B. japonicus DNA ($33.93{\pm}0.479$) for confirming B. albus. The methodology was validated by using ten commercial samples. The genomic DNA-based molecular technique that used the real-time PCR was a reliable method for the taxonomic classification of animal tissues.

Reproductive Cycle of Small Filefish, Rudarius ercodes (그물코쥐치, Rudarius ercodes의 생식주기)

  • LEE Taek Yuil;HANYU Isao
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.17 no.5
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    • pp.423-435
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    • 1984
  • The reproductive cycle of the small filefish, Rudarius ercodes was investigated based on the annual variations of gonadosomatic index(GSI) and hepatosomatic index(HSI) by electronic and photic microscophy. The specimens used were collected at the coastal area of Benden island, Sizuokagen, Japan, from September 1982 to August 1983. GSI began to increase from March, starting season of longer daylength and higher water temperature, and reached the maximum value between June and August. It began to decrease from September with the lowest value appearing between November and February without any evident variation. The annual variations of HSI were not distinct in male filefish and were negatively related to GSI in female : HSI decreased in the summer season when the ovary was getting mature and reached the maximum in the winter season when the ovary was getting retrogressive. The ovary consisted of a pair of saccular structure with numerous ovarian sacs branched toward the median cavity. Oogonia divided and proliferated along the germinal epithelium of the ovarian sac. Young oocytes with basophile cytoplasm showed several scattering nucleoli along the nuclear membrane. when the oocytes growing to about 300 ${\mu}m$, nuclear membrane to disappear with nucleus migrating toward the animal pole. The regions of protoplasm were extremely confined within the animal hemisphere in which most of cytoplasms were filled with yolk materials and oil drops. After ovulation, residual follicles and growing oocytes remaining in the ovarian sacs degenerated. But perinucleatic young oocytes without follicles formed were not degenerated, and growing continuously still in the next year. Mitochondria and endoplasmic reticula in the cytoplasm remarkably increased with oocytes maturing and yolk accumulating. Those were considered to be functionally related to the yolk accumulation. Five or six layers of possible vitellogenin, oval-shaped disc structures with high electron density, appeared in the apex of follicular processes stretching to the microvilli pits of mature oocytes. Testis consisting of a pair of lobular structures in the right and left were united in the posterior seminal vesicle, Cortex of testis was composed of several seminiferous tubules, and medulla consisting of many sperm ducts connected with tubules. Steroid hormone-secreting cells with numerous endoplasmic reticula and large mitochondria of well developed cristae were recognized in the interstitial cells of the growing testis. Axial filament of spermatozoon invaginated deeply in the central cavity of the nucleus and the head formed U-shape with acrosome severely lacking, mitochondria formed large globular paranuclei at the posterior head, and microtubular axoneme of the tail represented 9+9+2 type. The annual reproductive cycles could be divided into five successive stages : growth(March to July), maturation(May to September), Spawning(mid May to early October) and resting stages(October to February). The spawning peak occurred from June to August.

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Soil CO2 Efflux and Leaf-Litter Decomposition of Quercus variabilis and Pinus densiflora Stands in the Southern Region of Korean Peninsular

  • Kim, Sung Bin;Jung, Nam Chul;Lee, Kye-Han
    • Journal of Korean Society of Forest Science
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    • v.98 no.2
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    • pp.183-188
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    • 2009
  • It is necessary to determine the amount of carbon dioxide ($CO_2$) absorbed by plants and released from forest floor into atmosphere, to gain a better understanding how forests participate in the global carbon cycle. Soil $CO_2$ efflux, litter production, and decomposition were investigated in Q. variabilis and P. densiflora stands in the vicinity of Gwangju, Chonnam province. Soil $CO_2$ efflux was measured using Infrared Gas Analyzer (IRGA) at midday of the 10th day at every month over 12-month period, to quantify seasonal and annual budgets of soil $CO_2$ efflux. Soil temperature and soil moisture were measured at the same time. Seasonal soil $CO_2$ efflux in Q. variabilis and P. densiflora were the highest in summer season. In August, maximum soil $CO_2$ efflux in Q. variabilis and P. densiflora was 7.49, $4.61CO_2{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, respectively. Annual $CO_2$ efflux in each stand was 1.77, $1.67CO_2kg{\cdot}m^{-2}$ respectively. Soil $CO_2$ efflux increased exponentially with soil temperature and related strongly in Q. variabilis ($r^2$=0.96), and in P. densiflora ($r^2$=0.91). Litter production continued throughout the year, but showed a peak on November and December. Annual litter production in the Q. variabilis and P. densiflora stands were $613.7gdw{\cdot}m^{-2}{\cdot}yr^{-1}$ and $550.5gdw{\cdot}m^{-2}{\cdot}yr^{-1}$.$yr^{-1}$, respectively. After 1 year, % remaining mass of Q. variabilis and P. densiflora litter was 48.2, 57.1%, respectively. The soil $CO_2$ efflux rates in this study showed clear seasonal variations. In addition, the temporal variation in the $CO_2$ efflux rates was closely related to the soil temperature fluctuation rather than to variations in the soil moisture content. The range of fluctuation of soil $CO_2$ efflux and litter decomposition rate showed similar seasonal changes. The range of fluctuation of soil $CO_2$ efflux and litter decomposition rate was higher during summer and autumn than spring and winter.

The Morphological Changes of Deltaic Barrier Islands in the Nakdong River Estuary after the Construction of River Barrage (하구둑 건설 이후 낙동강 하구역 삼각주 연안사주의 지형변화)

  • Kim, Sung-Hwan
    • Journal of the Korean Geographical Society
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    • v.40 no.4 s.109
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    • pp.416-427
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    • 2005
  • This paper aims to investigate morphological changes of deltaic barrier islands in the Nakdong Estuary and especially their spatial variations after barrage construction. We analyzed shorelines, geometrical centroids, and areas to reveal the changes of barrier islands. Here, we suggest three interesting points from this study. First, each individual barrier island in the Nakdong estuary goes through a different stage of the geomorphic cycle. The frontal barrier islands such as Sinja-do and Doyo-deung grow because they are located in front of the gates of the barrage. Sediments in water out of the gates are moved to offshore and then reworked by coastal processes such as waves and tides. Second, on the contrary, Baekhap-deung located behind Doyo-deung now diminishes indicating that sediments mainly move to the frontal growing island. Third, there is no morphological change in several barrier islands far away from the main flow of the Nakdong river such as Jinwoo-do, Daema-deung, and Jangja-do. In conclusion, barrier islands in the Nakdong estuary show distinct spatial variations. As a barrier island is closer to the main channel or is in the frontal location, there happens a very dynamic change in the morphology of the island.

Diel Horizontal Migration of Planktonic Copepods in the Surf Zone of Yongil Bay, Korea (영일만 쇄파대에 나타나는 부유성 요각류의 주야 수평이동)

  • Suh Hae-Lip;Yu Ok-Hwan
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.29 no.4
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    • pp.527-536
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    • 1996
  • The patterns of diel horizontal migration (DHM) of 7 copepod species are compared as part of a general investigation of the zooplankton adaptations to the surf zone habitats. In a sandy shore surf zone of Yongil Bay, 3 sites such as the bottom and surface of 1 m water depth and water's edge are sampled with a sledge net(n=108). The surf zone copepod assemblage is dominated by 7 species; Acartia hudsonica, Fseudodiaptomus marinus, Paracalanus indicus, Calanus sinicus, Oithona similis, Sinocalanus tenellus and Labidocera bipinnata. Threefold variations in copepod abundance are observed within a diel cycle. Abundances of 7 dominant species and total copepods captured in the surface exhibit significant diel differences, but those taken in the bottom are not significantly affected by diel period. It is shown that about $90\%$ of the surf zone copepods performed DHM. The nocturnal high densities of copepods occurred for a neap tide when the offshore winds prevailed, suggesting the animals' ability for horizontal orientation and an active locomotion without invoking passive transportation by currents. Photoreactive behavior of copepods triggered by relative changes in light intensity may be a primary factor inducing DHM by aggregating in the surf zone during the night and spreading out at day; then copepods may reduce encounters with visual predators. In A. hudsonica, ontogenetic variations in timings of DHM are evident. Such variations are likely to minimize intraspecific competition for diets. Data on shoreward migration of copepods indicate that A. hudsonica, P. indicus, O. similis and S. tenellus can maintain swimming velocities of about $20m\;h^{-1}$ for durations of more than an hour. Our observations of strong diel difference in abundances point out the need for both day and night samplings in surf zone habitats, if the importance of these habitats to planktonic copepods are to be fully understood.

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The Variations of Stratospheric Ozone over the Korean Peninsula 1985~2009 (한반도 상공의 오존층 변화 1985~2009)

  • Park, Sang Seo;Kim, Jhoon;Cho, Nayeong;Lee, Yun Gon;Cho, Hi Ku
    • Atmosphere
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    • v.21 no.4
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    • pp.349-359
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    • 2011
  • The climatology in stratospheric ozone over the Korean Peninsula, presented in previous studies (e.g., Cho et al., 2003; Kim et al., 2005), is updated by using daily and monthly data from satellite and ground-based data through December 2009. In addition, long-term satellite data [Total Ozone Mapping Spectrometer (TOMS), Ozone Monitoring Instrument (OMI), 1979~2009] have been also analyzed in order to deduce the spatial distributions and temporal variations of the global total ozone. The global average of total ozone (1979~2009) is 298 DU which shows a minimum of about 244 DU in equatorial latitudes and increases poleward in both hemispheres to a maximum of about 391 DU in Okhotsk region. The recent period, from 2006 to 2009, shows reduction in total ozone by 6% relative to the values for the pre-1980s (1979~1982). The long-term trends were estimated by using a multiple linear regression model (e.g., WMO, 1999; Cho et al., 2003) including explanatory variables for the seasonal variation, Quasi-Biennial Oscillation (QBO) and solar cycle over three different time intervals: a whole interval from 1979 to 2009, the former interval from 1979 to 1992, and the later interval from 1993 to 2009 with a turnaround point of deep minimum in 1993 is related to the effect of Mt. Pinatubo eruption. The global trend shows -0.93% $decade^{-1}$ for the whole interval, whereas the former and the later interval trends amount to -2.59% $decade^{-1}$ and +0.95% $decade^{-1}$, respectively. Therefore, the long-term total ozone variations indicate that there are positive trends showing a recovery sign of the ozone layer in both North/South hemispheres since around 1993. Annual mean total ozone (1985~2009) is distributed from 298 DU for Jeju ($33.52^{\circ}N$) to 352 DU for Unggi ($42.32^{\circ}N$) in almost zonally symmetric pattern over the Korean Peninsula, with the latitudinal gradient of 6 DU $degree^{-1}$. It is apparent that seasonal variability of total ozone increases from Jeju toward Unggi. The annual mean total ozone for Seoul shows 323 DU, with the maximum of 359 DU in March and the minimum of 291 DU in October. It is found that the day to day variability in total ozone exhibits annual mean of 5.7% in increase and -5.2% in decrease. The variability as large as 38.4% in increase and 30.3% in decrease has been observed, respectively. The long-term trend analysis (e.g., WMO, 1999) of monthly total ozone data (1985~2009) merged by satellite and ground-based measurements over the Korean Peninsula shows increase of 1.27% $decade^{-1}$ to 0.80% $decade^{-1}$ from Jeju to Unggi, respectively, showing systematic decrease of the trend magnitude with latitude. This study also presents a new analysis of ozone density and trends in the vertical distribution of ozone for Seoul with data up to the end of 2009. The mean vertical distributions of ozone show that the maximum value of the ozone density is 16.5 DU $km^{-1}$ in the middle stratospheric layer between 24 km and 28 km. About 90.0% and 71.5% of total ozone are found in the troposphere and in the stratosphere between 15 and 33 km, respectively. The trend analysis reconfirms the previous results of significant positive ozone trend, of up to 5% $decade^{-1}$, in the troposphere and the lower stratosphere (0~24 km), with negative trend, of up to -5% $decade^{-1}$, in the stratosphere (24~38 km). In addition, the Umkehr data show a positive trend of about 3% $decade^{-1}$ in the upper stratosphere (38~48 km).

The Study of Coastal Change Detection Ortho Aerial Photo and Hydrographic Survey: Parcels Submerged (정사항공사진과 해양조사측량을 이용한 해안선변화 탐지에 관한 연구: 포락지 중심으로)

  • Choi Chul Eung;Kim Youn Soo;Suh Yong Cheol
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.17 no.2
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    • pp.61-69
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    • 2005
  • The coastal erosion and the look of a heap which are mainly occurred in the district along the coast are found for various forms such as the estuary closing, the estuary sand bar the development of the coast sand bar, and the modification of coastline. Recently, due to the coastal development, these transformations have been intensified. The change of coast, which has been made slow progress is required long-term study on a searching examination of the root cause and a suggestion of a counter measure. In this research, ortho aerial photos were produced to analyze volumes of topographical alternations that have been progressed fer the long run, by 10s cycle from 1940s through 1990s, to compute accurate volumes of coastline variation, through a datum point and G.C.P (Ground Control Point). Also in this study, without respect to water level, the coastline variation was analyzed by using comparatively analyzed a Idlest land map, a cadastral map. And to analyze topographical variation volumes, the tidal station's materials was used under consideration f3r tide. Finally, topographical variation volumes are comparatively analyzed through surveying and sounding and a point of fine of aviation photographing was calculated and revised. After this research, by using ortho aerial photos, We can understand efficiency of these in computing volumes of variations of coastline by analyzing quantitatively erosion and look of a heap. Besides, in the future, these will be used for information gathering of the coastline integration control system.

Research for Time Variation of $C_{20}$ Using GRACE and SLR Measurements (GRACE 및 SLR 자료를 이용한 $C_{20}$의 시계열 변화 연구)

  • Huang, He;Yun, Hong-Sic;Lee, Dong-Ha
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.26 no.5
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    • pp.513-518
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    • 2008
  • The research of global-scale mass redistribution and it changed by Earth gravity filed variation observations, including Earth's oblateness $J_2$(also called low degree spherical harmonic coefficient $C_{20}$), is in continuous progress. Recently, the comparative analysis of geodetic observation SLR can be made by the development of GRACE and other time-variable gravity measurements. In this study, $C_{20}$ time series changes in the value of comparative analysis was got by GRACE monthly Gravity filed model (CSR RL04) for the period April 2002 to May 2008. And comparative analysis the harmonic coefficients of $C_{20}$ was obtained from SLR observations. Signal analysis for two time-series data was made by wavelet transform, CWT(continuous wavelet transform), XWT(cross wavelet transform) and WTC(wavelet coherence) methods. The results indicate that GRACE and SLR values for $C_{20}$ had both decreasing trend, as well as SLR data represent the annual frequencies, and GRACE was semiannual variations. In addition, the results of GRACE and SLR had a strong correlation with the XWT and WTC in an annual cycle.

Variations in Nutrients and CO2 Uptake with Growth of Undaria pinnatifida from the South Coast of Korea (미역 (Undaria pinnatifida)의 생장에 따른 영양염과 CO2 흡수율 변화)

  • Shim, Jeong-Hee;Hwang, Jae-Ran;Lee, Jae-Seong;Kim, Jong-Hyun;Kim, Sung-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.43 no.6
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    • pp.679-686
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    • 2010
  • To investigate the contribution of macroalgae to biogeochemical nutrients and carbon cycles, we measured the uptake rates of nutrients and $CO_2$ by Undaria pinnatifida using an incubation method in an acrylic chamber. From January to March 2010, U. pinnatifida was sampled at Ilkwang, a well-known area of macroalgae culture in Korea. The initial and final concentrations of nutrients, dissolved oxygen, total alkalinity, and pH of the chamber water were measured, and production/uptake rates were calculated using concentration changes, chamber volume, and incubation time. The production rate of dissolved oxygen by U. pinnatifida (n = 32) was about $5.4{\pm}4.0\;{\mu}mol\;g_{fw}^{-1\;}h^{-1}$. The uptake rate of total dissolved inorganic carbon (TDIC), calculated by total alkalinity and pH, was $7.9{\pm}6.5\;{\mu}mol\;g_{fw}^{-1}\;h^{-1}$. Nutrients uptake averaged $141.7{\pm}119.2$ nmol N $g_{fw}^{-1}\;h^{-1}$ and $15.0{\pm}9.1$ nmol P $g_{fw}^{-1}\;h^{-1}$. A positive linear correlation ($r^2$ = 9.6) existed between the production rate of dissolved oxygen and the uptake rate of total dissolved inorganic carbon, suggesting that these two factors serve as good indicators of U. pinnatifida photosynthesis. The relationships between fresh weight and uptake rates of nutrients and $CO_2$ suggested that younger specimens (<~50 g fresh weight) are much more efficient at nutrients and $CO_2$ uptake than are specimens >50 g. The amount of carbon uptake by the total biomass of U. pinnatifida in Korea during the year of 2008 was about 0.001-0.002% of global ocean carbon uptake. Thus, more research should be focused on macroalgae-based biogeochemical cycles to evaluate the roles and contributions of macroalgae to the global carbon cycle.

Controlled Reproductive Cycle of Rockfish (Sebastes inermis) by Water Temperature and Photoperiod (수온과 광주기에 의한 볼락(Sebastes inermis)의 번식주기 조절)

  • KO Chang Soon;CHANG Young Jin;LIM Han Kyu;KIM Jong-Hyun;CHO Kee Chae
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.5
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    • pp.713-720
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    • 1998
  • Experiments on the control of rockfish, Sebastes inermis reproduction were performed by artificially changed water temperature (WT) and photoperiod (PP). The experiments were divided into three conditions; artificial changes of both WT and PP delayed two months against their natural variations (Exp.I), natural WT and PP replaced with artificial light (Exp.II) and natural WT and PP (Exp.III) from September to April (220 days). Initial gonadosomatic index (GSI) in female groups of experiments was 0.2. Female GSI of responded group in Exp.I began to increase from December and reached the highest value in February with 15.0, while the un-responded group kept the lower value during the experimental period. Female GSI of Exp.II and Exp.III reached the highest values in January with 13.0 and 14.7, respectively. Male GSI of Exp,I reached the maximum in October, and that of Exp.II and III reached the maximum in November and then rapidly decreased. Among the experimental groups, male GSI did not show the different tendency. Monthly changes of hepatosomatic index in both sexes showed the reciprocal tendency with those of GSI. The control of WT and PP did not affect on the changes of HSI and condition factor of rockfish. From the histological observations, maturation of ovary was delayed by the changes of WT and PP, but that of testis was not influenced. Judging from the above results, the maturation and parturition of female rockfish could be delayed one or two months by artificial changes of WT and PP.

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