• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.36-36
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• 2017

Out of twenty common protein amino acids, there are many kinds of non protein amino acids (NPAAs) that exist as secondary metabolites and exert ecological functions in plants. Mimosine (Mim), one of those NPAAs derived from L. leucocephala acts as an iron chelator and reversely block mammalian cell cycle at G1/S phases. Cysteine (Cys) is decisive for protein and glutathione that acts as an indispensable sulfur grantor for methionine and many other sulfur-containing secondary products. Cys biosynthesis includes consecutive two steps using two enzymes-serine acetyl transferase (SAT) and O-acetylserine (thiol)lyase (OASTL) and appeared in plant cytosol, chloroplast, and mitochondria. In the first step, the acetylation of the ${\beta}$-hydroxyl of L-serine by acetyl-CoA in the existence of SAT and finally, OASTL triggers ${\alpha}$, ${\beta}$-elimination of acetate from OAS and bind $H_2S$ to catalyze the synthesis of Cys. Mimosine synthase, one of the isozymes of the OASTLs, is able to synthesize Mim with 3-hydroxy-4-pyridone (3H4P) instead of $H_2S$ for Cys in the last step. Thus, the aim of this study was to clone and characterize the cytosolic (Cy) OASTL gene from L. leucocephala, express the recombinant OASTL in Escherichia coli, purify it, do enzyme kinetic analysis, perform docking dynamics simulation analysis between the receptor and the ligands and compare its performance between Cys and Mim synthesis. Cy-OASTL was obtained through both directional degenerate primers corresponding to conserved amino acid region among plant Cys synthase family and the purified protein was 34.3KDa. After cleaving the GST-tag, Cy-OASTL was observed to form mimosine with 3H4P and OAS. The optimum Cys and Mim reaction pH and temperature were 7.5 and $40^{\circ}C$, and 8.0 and $35^{\circ}C$ respectively. Michaelis constant (Km) values of OAS from Cys were higher than the OAS from Mim. Inter fragment interaction energy (IFIE) of substrate OAS-Cy-OASTL complex model showed that Lys, Thr81, Thr77 and Gln150 demonstrated higher attraction force for Cys but 3H4P-mimosine synthase-OAS intermediate complex showed that Gly230, Tyr227, Ala231, Gly228 and Gly232 might provide higher attraction energy for the Mim. It may be concluded that Cy-OASTL demonstrates a dual role in biosynthesis both Cys and Mim and extending the knowledge on the biochemical regulatory mechanism of mimosine and cysteine.

• Journal of the Korean Society for Nondestructive Testing
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• v.35 no.2
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• pp.141-149
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• 2015

In the fabrication of electronic circuits used in electronic products, molybdenum thin films are deposited on semiconductors to prevent oxidation. During the deposition, the presence of a particle or dust at the interface between the thin film and substrate causes the decrease of adhesion, performance, and life cycle. In this study, a damage measurement targeting two kinds of glass substrate, with and without particles, was performed in order to measure the change in the molybdenum thin film deposition area in the presence of a particle. Clean and dirty molybdenum thin film specimens were fabricated and directly deposited on a substrate using the sputtering method, and a reflection-type digital holographic interferometer was configured for measuring the damage. Reflection-type digital holography has several advantages; e.g., the configuration of the interferometer is simple, the measurement range can be varied depending on the magnification of a microscopic lens, and the measuring time is short. The results confirm that reflection-type digital holography is useful for the measurement of the damage and defects of thin films.

• Journal of Korean Society of Transportation
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• v.33 no.5
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• pp.478-487
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• 2015

This study proposed a Variable Speed Limits(VSL) algorithm to use traffic information based on Cumulative Demand-Capacity Analysis and evaluated its performance. According to the analysis result, the total of delay consisted of 3 separate parts. There was no change in total travel time although the total of delay decreased. These effects was analysed theoretically and then, evaluated through VISSIM, a microscopic simulator. VISSIM simulation results show almost same as those of theoretical analysis. Furthermore in SSAM analysis with VISSIM simulation log, the number of high risk collisions decreased 36.0 %. However, the total delay decrease effect is not real meaning of decrease effect because the drivers' desired speed is same whether the VSL model is operated or not. Nevertheless this VSL model maintains free flow speed for longer and increases the cycle of traffic speed fluctuation. In other words, this is decrease of delay occurrence and scale. The decrease of speed gap between upstream and downstream stabilizes the traffic flow and leads decrease number of high risk collision. In conclusion, we can expect increase of safety through total delay minimization according to this VSL model.

• Journal of the Korean Ceramic Society
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• v.45 no.12
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• pp.839-844
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• 2008

$TiO_2$ is an environment-friendly semiconducting material, and it has photocatalytic and hydrophilic effect. There are a lot of reports on the photocatalytic characteristics of $TiO_2$, such as organic pollutants resolving, anti-bacterial, and self-purification material. In this paper, $TiO_2$ micron-sized powders were deposited on the glass by room temperature powder spray in vacuum process, so called aerosol deposition (AD), and nano-grained $TiO_2$ photocatalytic thin films were fabricated. The thickness of the films were controlled by changing the number of deposition cycle. Morphologies and characteristics of the AD-$TiO_2$ thin films were examined by SEM, TEM, XRD, and UV-Visible Spectrophotometer. As the thickness of $TiO_2$ films increased, surface roughness increased. By this increment, the reaction area between film and pollutant was enlarged, resulting in better photocatalytic property.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.47 no.1
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• pp.20-27
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• 2010

This paper describes a design of 5-stage pipelined de-blocking filter with power reduction scheme and proposes a efficient memory architecture and filter order for high performance H.264/AVC Decoder. Generally the de-blocking filter removes block boundary artifacts and enhances image quality. Nevertheless filter has a few disadvantage that it requires a number of memory access and iterated operations because of filter operation for 4 time to one edge. So this paper proposes a optimized filter ordering and efficient hardware architecture for the reduction of memory access and total filter cycles. In proposed filter parallel processing is available because of structured 5-stage pipeline consisted of memory read, threshold decider, pre-calculation, filter operation and write back. Also it can reduce power consumption because it uses a clock gating scheme which disable unnecessary clock switching. Besides total number of filtering cycle is decreased by new filter order. The proposed filter is designed with Verilog-HDL and functionally verified with the whole H.264/AVC decoder using the Modelsim 6.2g simulator. Input vectors are QCIF images generated by JM9.4 standard encoder software. As a result of experiment, it shows that the filter can make about 20% total filter cycles reduction and it requires small transposition buffer size.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.1 no.2
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• pp.125-136
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• 1997

Digestion of a municipal wastewater sludge by the anaerobic sequencing batch reactor(ASBR) was investigated to evaluate the performance of the ASBR process at a critical condition of high-solids-content feed. The reactors were operated at an HRT of 10 days with an equivalent loading rate of 0.8-1.5 gVS/L/d at $35^{\circ}C.$ The main conclusions drawn from this study were as follows: 1. Digestion of a municipal wastewater sludge was possible using the ASBR in spite of high concentration of settleable solids in the sludge. The ASBRS with 3- and 4-day cycle period showed almost identical high digestion performances. 2. No adverse effect on digestion stability was observed in the ASBRS in spite of withdrawal and replenishment of $30\%\;or\;40\%$ of liquid contents. A conventional anaerobic digester could be easily converted to the ASBR without any stability problem. 3. Flotation thickening occurred in thicken step of the ASBRS throughout steady state, and floating bed volume at the end of thicken period occupied about $70\%$ of the working volume of the reactor. Efficiency of flotation thickening in the ASBRS could be comparable to that of additional gravity thickening of a completely mixed digester. 4. Solids were accumulated rapidly in the ASBR during start-up period. Solids concentrations in the ASBRS were 2.6 times higher than that in the completely mixed control reactor at steady state. Dehydrogenase activity had a strong correlation with the solids concentration. Dehydrogenase activity of the digested sludge in the ASBR was 2.9 times higher than that of the sludge in the control reactor, and about 25 times higher than that of the subnatant in the ASBR. 5. Remarkable increase in equivalent gas production of $52\%$ was observed at the ASBRS compared with the control reactor in spite of similar Quality of clarified effluent from the ASBRS and control reactor. The increase in gas production from the ASBRS was believed to be combined results of accumulation of microorganisms, higher driving force applied, and additional long-term degradation of organics continuously accumulated.

• Journal of The Korean Association For Science Education
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• v.33 no.4
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• pp.794-806
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• 2013

The purpose of this study is to analyze what kinds of science education theories are targeted in the "Examination for Appointing Secondary School Science Teachers (EASST)." For the analyses, we extracted the contents related to the science education theories in the question items of the EASST of 2008 through 2012, and categorized those theories into science curriculum, history of science and philosophy of science, scientific inquiry, theory of teaching and learning, model of teaching and learning, and assessment. The results of this study indicated that the theory of teaching and learning appeared most frequently and there were high proportions of question items related to the following topics: contents in science curriculum, scientific method, contemporary philosophy of science, process of inquiry, Ausubel's theory, learning cycle model by Lawson, cooperative learning, criteria of performance assessment, and etc. While we, as science educators, believed that the other categories such as 'history of science' provides important topics for pre-service science teachers, questions items dealing with those were rarely found in the past EASSTs. As EASST has strong influences on the professional developments of pre-service science teachers, more research should be pursued on how much and what domains of science education theories would be appropriate for the test.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.10
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• pp.248-258
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• 2020

This study proposed a breakdown structure for maintenance and management technologies under the concept of comprehensive asset management at the life cycle level of infrastructure based on benchmarking with other developed countries. For this purpose, a comparative case study was performed to review and analyze the existing definitions and hierarchies for infrastructure maintenance, repair, and rehabilitation (MR&R) systems under major industrialized countries and South Korea. In accordance with the ratio of maintenance costs to GDP, the U.S., U.K, and Japan were selected to review their systems. The classifications and definitions of MR&R technologies under the laws were analyzed. The result showed that most developed countries differentiate maintenance and repair from improvement and constitute a system centered on preventive maintenance activities. On the other hand, Korea's system for facility management is not definitely classified and still focused on reactive structures, which need to be improved. In this study, as proposed, a breakdown structure established the concept of Maintenance and Management, Maintenance & Repair, and Performance Improvement. Consequently, this study could be used as the basis for the implementation of preventive MR&R activities and reasonable resource allocations from an asset management point of view.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.40 no.3
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• pp.127-138
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• 2016

Diesel vehicles should be equipped with urea-selective catalytic reduction(SCR) system as a high-performance catalyst, in order to reduce harmful nitrogen oxide emissions. In this study, a three-dimensional Eulerian-Lagrangian CFD analysis was used to numerically predict the multiphase flow characteristics of the urea-SCR system, coupled with the chemical reactions of the system's transport phenomena. Then, the numerical spray structure was modified by comparing the results with the measured values from spray visualization, such as the injection velocity, penentration length, spray radius, and sauter mean diameter. In addition, the analysis results were verified by comparison with the removal efficiency of the nitrogen oxide emissions during engine and chassis tests, resulting in accuracy of the relative error of less than 5%. Finally, a verified CFD analysis was used to calculate the interanl flow of the urea-SCR system, thereby analyzing the characteristics of pressure drop and velocity increase, and predicting the uniformity index and overdistribution positions of ammonia.

• Journal of the Korea Institute of Building Construction
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• v.10 no.5
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• pp.45-54
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• 2010

Recently more and more construction projects have become high-rise, complex and intelligent. Accordingly, such projects require an integrated management system for tasks, with a lean approach to construction with work processes for management and productivity. In particular, Construction Information Technology (CIT) fields are concerned with Building Information Modeling (BIM), which represents the process of generating and managing building data during its life cycle. Constructability research has progressed for the project goal which is a cost-time-quality of optimization by integrated construction knowledge and experience. However, the current constructability process has not been performed efficiently, as the existing 2D drawings and papers lack consistent and accurate information, it is difficult to share the contents of work, and the use of information is inefficient. This study proposes that the reformation and enhancement of BIM-based constructability work process can lead to brilliant performance in the framework of the construction phase through achieving collaboration between the design team and the workers at the site.