• BMB Reports
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• 제41권7호
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• pp.542-547
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• 2008

D-type cyclins control the onset of cell division and the response to extracellular signals during the G1 phase. In this study, we transformed a D-type cyclin gene, Nicta;CycD3;4, from Nicotiana tabacum using an Agrobacterium-mediated method. A predicted 1.1 kb cyclin gene was present in all of the transgenic plants, but not in wild-type. Northern analyses showed that the expression level of the Nicta;CycD3;4 gene in all of the transgenic plants was strong when compared to the wild-type plants, suggesting that Nicta;CycD3;4 gene driven by the CaMV 35S promoter was being overexpressed. Our results revealed that transgenic plants overexpressing Nicta;CycD3;4 had an accelerated growth rate when compared to wild-type plants, and that the transgenic plants exhibited a smaller cell size and a decreased cell population in young leaves when compared to wild-type plants.

• 미생물학회지
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• 제46권2호
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• pp.122-126
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• 2010

RAD53은 효모의 검문지점 경로가 DNA 손상을 감지하여 여러 가지 후속적인 세포 내 반응을 일으키는 데 핵심적인 역할을 하는 인산화 효소일 뿐만 아니라, dNTP 생성에 중요한 RNR 유전자 등의 전사 활성화 과정에도 관여하는 효모의 생존에 필수적인 유전자이다. 본 연구에서는 rad53${\Delta}$ 돌연변이의 hydroxyurea에 대한 민감성을 억제하는 억제자로서 CYC8을 동정하였다. CYC8 유전자가 많은 사본으로 존재할 때 rad53${\Delta}$ 균주의 hydroxyurea에 대한 내성이 증가하였으나, CYC8과 복합체로 작용하는 TUP1은 다사본 억제자로 작용하지 못하였다. 반면, 삭제 돌연변이의 경우, cyc8${\Delta}$과 tup1${\Delta}$ 모두 억제자로 작용하였다. CYC8은 효모에서 프리온 단백질로 작용하기 때문에 과량 발현되면 정상적인 CYC8 단백질의 잘못된 접힘을 유발하게 되고, 결과적으로 우성의 $cyc8^-$ 표현형이 나타나게 된다. 따라서 CYC8이 다사본 억제자로 작용하는 이유는 이러한 프리온의 특성 때문으로 추측된다. CYC8이 다사본이거나 cyc8${\Delta}$ 돌연변이일 경우 모두 RNR 유전자의 전사가 증가되는 것을 관찰하였다. 따라서 CYC8에 의한 rad53${\Delta}$ 돌연변이의 억제는 RNR 증가에 따른 세포 내 dNTP 증가 때문으로 생각된다.

• Plant Biotechnology Reports
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• 제2권4호
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• pp.227-231
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• 2008

D-class cyclins play important roles in controlling the cell cycle in development and in response to external signals by forming the regulatory subunit of cyclin-dependent kinase (CDK) complexes. To evaluate the effects of D-class cyclins in transgenic rice plants, Arabidopsis cyclin D2 gene (CycD2) was linked to the maize ubiquitin1 promoter (Ubi1) and introduced into rice by the Agrobacterium-mediated transformation method. Genomic deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and Western blot hybridizations of the Ubi1:-CycD2 plants revealed copy number of transgene and its increased expression in leaf and callus cells at messenger RNA (mRNA) and/or protein levels. The H1 kinase assay using the immunoprecipitates of protein extracts from the Ubi1:CycD2 plants and nontransgenic controls demonstrated that the introduced Arabidopsis CycD2 forms a functional CycD2/CDK complex with an unidentified CDK of rice. Shoot and root growth was enhanced in the Ubi1:CycD2 seedlings compared with nontransgenic controls, together, suggesting that Arabidopsis cyclin D2 interacts with a rice cyclin-dependent kinase, consequently enhancing seedling growth.

• Journal of Plant Biotechnology
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• 제30권4호
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• pp.329-334
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• 2003

D-type cyclin은 호르몬과 영양분의 영향을 받아 세포주기의 G1기에서 S기로 전환을 조절하는 인자이다. 우리는 감자에서 이 유전자를 분리 해냈고, 염기서열 분석을 통하여 D3 cyclin으로 분류하였다. 그리고 StCycD3;1이라 명명하였다. 다른 D cyclin유전자가 세포 분열이 활발한 조직에서 발현되는 것과 같이 StCycD3;1은 감자의 괴경, 뿌리, 꽃, 잎, 줄기, 뿌리줄기, 복지에서 다양한 발현 양상을 보였고, 영양분의 하나인 sucrose에 의하여 발현이 유도되는 것을 확인하였다.

• Journal of Photoscience
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• 제9권2호
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• pp.240-242
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• 2002

In Drosophila melanogaster, it is known that the circadian clock consists of an autoregulatory feedback loop, which includes so-called clock genes, such as per, tim, dClk and cyc and produces periodical expression of per. It is recently suggested, however, that the circadian oscillation without the rhythmical expression of per is involved in the regulation of circadian locomotor rhythms. In the present study, we examined the existence and the property of the possible per-less oscillation using arrhythmic clock mutant flies carrying per$^{01}$, tim$^{01}$, dClk$^{Jrk}$ or cyc$^{01}$. When temperature cycles consisting of 25$^{\circ}$Ｃ and 30$^{\circ}$Ｃ with varying periods (T = 8~32 hr) were given, they showed rhythms synchronizing with the given cycle under constant darkness (DD). per$^{01}$ and tim$^{01}$ flies always showed a peak around 7 hr after the onset of thermophase irrespective of Ts of temperature cycles, while dClk$^{Jrk}$ and cyc$^{01}$ flies did not. In addition, several days were necessary to establish a clear temperature entrainment in per$^{01}$ and tim$^{01}$ flies, when they were transferred from a constant temperature to a temperature cycle under DD. These results suggest that per$^{01}$ and tim$^{01}$ flies have a temperature-entrainable weak oscillatory mechanism. The fact that dClk$^{Jrk}$ and cyc$^{01}$ flies did not show any sign of the endogenous oscillation suggests that the per-less oscillatory mechanism may require CLK and CYC.

• Geomechanics and Engineering
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• 제37권2호
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• pp.123-133
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• 2024

This study investigates the effects of loading frequency (f) and specimen size on the liquefaction resistance of clean sand. A series of cyclic direct simple shear tests were conducted on Jumunjin sand with varying consolidated relative densities (40% and 80%), f values (0.05, 0.10, and 0.20 Hz), and diameter to height (D/H) ratios (3.63, 3.18, 2.82, and 2.54). The results demonstrated the significant influence of f and D/H ratio on the number of cycles to liquefaction (N_cyc-liq) and the cyclic resistance ratio (CRR₁₅). It was observed that increasing f linearly increased N_cyc-liq. Increasing the specimen height also led to higher N_cyc-liq values irrespective of the f or relative density. Moreover, a positive correlation between CRR₁₅ and f indicated that higher f yielded higher CRR₁₅. This relationship was more pronounced in dense sand than in loose sand. Specimen height also significantly affected CRR₁₅, with increasing the specimen height resulting in higher CRR₁₅ values. Furthermore, the effect of f on CRR₁₅ was less significant compared to the influence of specimen height. The effect of f on the normalized cyclic resistance ratio (NCRR) was relatively negligible for loose sand but more substantial for dense sand depending on the D/H ratio. Data analysis revealed that the NCRR generally decreases as the D/H ratio increases. An interpolation formula was provided to calculate the NCRR based on the D/H ratio regardless of the f and relative density.

• BMB Reports
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• 제49권11호
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• pp.587-589
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• 2016

The circadian clock system enables organisms to anticipate the rhythmic environmental changes and to manifest behavior and physiology at advantageous times of the day. Transcriptional/translational feedback loop (TTFL) is the basic feature of the eukaryotic circadian clock and is based on the rhythmic association of circadian transcriptional activator and repressor. In Drosophila, repression of dCLOCK/CYCLE (dCLK/CYC) mediated transcription by PERIOD (PER) is critical for inducing circadian rhythms of gene expression. Pacemaker neurons in the brain control specific circadian behaviors upon environmental timing cues such as light and temperature cycle. We show that amino acids 657-707 of dCLK are important for the transcriptional activation and the association with PER both in vitro and in vivo. Flies expressing dCLK lacking AA657-707 in $Clk^{out}$ genetic background, homologous to the mouse Clock allele where exon 19 region is deleted, display pacemaker-neuron-dependent perturbation of the molecular clockwork. The molecular rhythms in light-cycle-sensitive pacemaker neurons such as ventral lateral neurons ($LN_vs$) were significantly disrupted, but those in temperature-cycle-sensitive pacemaker neurons such as dorsal neurons (DNs) were robust. Our results suggest that the dCLK-controlled TTFL diversify in a pacemaker-neuron-dependent manner which may contribute to specific functions such as different sensitivities to entraining cues.

• Journal of Microbiology and Biotechnology
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• 제16권11호
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• pp.1678-1683
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• 2006

A novel suspension-phase enzyme reaction system for the intermolecular transglycosylation of L-ascorbic acid into 2-O-${\alpha}$-D-glucopyranosyl L-ascorbic acid supplementing extrusion starch as the glycosyl donor was developed using cyclodextrin glucanotransferase from Thermoanaerobacter sp. A high conversion yield compared to the conventional soluble-phase enzyme reaction system using cyclodextrins and soluble starch was achieved. The optimal reaction conditions were 2,000 units of cycIodextrin glucanotransferase, 20 g/l of L-ascorbic acid, and 50 g/l of extrusion starch at $50^{\circ}C$ for 24 h. The new suspension-phase enzyme reaction system also exhibited several distinct advantages other than a high conversion yield, including a lower accumulation of oligosaccharides and easily separable residual extrusion starch by centrifugation or filtration in the reaction mixture, which will facilitate the purification of 2-O-${\alpha}$-D-glucopyranosyl L-ascorbic acid. The new suspension-phase enzyme reaction system seems to be potentially applicable as the industrial process for the production of thermally and oxidatively stable 2-O-${\alpha}$-D-glucopyranosyl L-ascorbic acid.

• 한국안전학회지
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• 제7권2호
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• pp.47-56
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• 1992

The retardation effect of fatigue crack propagation after cyclic overloading seems to be affected by strain hardening exponent. Namely, for the material with high values of n, the delay effect is found to be severe. We proposed a modified crack retardation equation which may apply the retardation of fatigue crack growth after a cyclic overloading, as (da/dN)'$_{cyc}$=($\mu$n＋λ)B $\Delta$ $K^{q}$ /[(1－ $R_{eff}$) $K_{cf}$ －$\Delta$K]. where, $R_{eff}$ is effective stress ratio [＝( $K_{min}$－K, os)/( $K_{max}$－ $K_{res}$)] The constants $\mu$＝－0.5 and λ＝0.6, and the values are found to be identical for materials such as aluminum (A 1060), steel (SS 34), brass ( $B_{s}$ SIB) and stainless steel (SUS 304) used in this investigation. (SUS 304) used in this investigation.ation.n.n.

• Molecules and Cells
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• 제20권1호
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• pp.136-141
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• 2005

Mild stresses such as high temperature ($30^{\circ}C$) or a low $H_2O_2$ concentration induced transient cell cycle arrest at G1/S or G2/M depending on the cell cycle stage at which the stress was applied. When stresses were introduced during G0 or G1, the G1/S checkpoint was mainly used; when stresses were introduced after S phase, G2/M was the primary checkpoint. The slowing of cell cycle progression was associated with transient delays in expression of A-, B-, and D-type cyclins. The delay in expression of NtcycA13, one of the A-type cyclins, was most pronounced. The levels of expression of Ntcyc29 (a cyclin B gene) and of CycD3-1 differed most depending on the applied stress, suggesting that different cellular adjustments to mild heat and a low concentration of $H_2O_2$ are reflected in the expression of these two cyclins.