Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Isolation and Characterization of a cDNA Encoding CycD3 Gene from Potato(Solanum tuberosum L.)

감자 (Solanum tuberosum L.) CycD3유전자의 분리 및 특성 분석

  • Kang, In-Hong (Department of Genetic engineering, Sungkyunkwan University) ;
  • Choi, Seung-Ho (Department of Genetic engineering, Sungkyunkwan University) ;
  • Lee, Hong-Geun (Department of Genetic engineering, Sungkyunkwan University) ;
  • Hwang, Hyun-Sik (Department of Genetic engineering, Sungkyunkwan University) ;
  • Lee, Suk-Chan (Department of Genetic engineering, Sungkyunkwan University) ;
  • Jung, Tae-Young (Department of Genetic engineering, Sungkyunkwan University) ;
  • Lim, Hak-Tae (Department of Biotechnology, Kangwon University) ;
  • Bae, Shin-Chul (National Institute of Agricultural Biotechnology, RDA)
  • 강인홍 (성균관대학교 유전공학과) ;
  • 최승호 (성균관대학교 유전공학과) ;
  • 이홍근 (성균관대학교 유전공학과) ;
  • 황현식 (성균관대학교 유전공학과) ;
  • 이석찬 (성균관대학교 유전공학과) ;
  • 정태영 (성균관대학교 유전공학과) ;
  • 임학태 (강원대학교 생명공학부) ;
  • 배신철 (농업생명공학연구원)
  • Published : 2003.12.01
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Abstract

D-type cyclins are believed to regulate the G1 to S phase transition in response to nutrient and hormonal signals. We investigated the expression characteristics of the key cell-cycle regulators, mitotic and G1 cyclins in potato (Solanum tuberosum L.). We isolated D-type cyclin gene from potato and it was classified as D3 cyclin by sequence similarities and a phylogenetic analysis, and named as StcycD3;1. The accumulation of transcripts was predominantly associated with mitotically active organs, such as stolons, roots, flowers, leaves, and stems. Transcription of StcycD3;1 can be induced by sucrose.

D-type cyclin은 호르몬과 영양분의 영향을 받아 세포주기의 G1기에서 S기로 전환을 조절하는 인자이다. 우리는 감자에서 이 유전자를 분리 해냈고, 염기서열 분석을 통하여 D3 cyclin으로 분류하였다. 그리고 StCycD3;1이라 명명하였다. 다른 D cyclin유전자가 세포 분열이 활발한 조직에서 발현되는 것과 같이 StCycD3;1은 감자의 괴경, 뿌리, 꽃, 잎, 줄기, 뿌리줄기, 복지에서 다양한 발현 양상을 보였고, 영양분의 하나인 sucrose에 의하여 발현이 유도되는 것을 확인하였다.

Keywords

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