• Journal of Microbiology and Biotechnology
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• 제11권4호
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• pp.636-643
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• 2001

Bacillus sp. AIR-5, a strain from soil, produced an extracellular maltopentaose-forming amylase from amylose and soluble starch. This bacterium produced 8.9 g/l of maltopentaose from 40 g/l of soluble starch in a batch fermentation and the maltopentaose made up 90 % of the maltooligosaccharides produced (from maltose to maltoheptaose). The culture supernatant was concentrated using a 30 K molecular weight cut-off membrane and purified by DEAE-Cellulose and Sephadex G-150 column chromatographies. The purified protein showed one band on a native-PAGE and its molecular mass was estimated as 250 kDa. The 250-kDa protein was composed of tetramers of a 63-kDa protein. the isoelectric point of the purified protein was pH 6.9, and the optimum temperature for the enzyme activity was $45^{\circ}C$. The enzyme was quickly inactivated above $55^{\circ}C$, and showed a maximum activity at pH 8.5 and over 90% stability between a pH of 6 to 10. The putative N-terminal amino acid sequence of AIR-5 amylase, ATINNGTLMQYFEWYVPNDG, showed a 96% sequence similarity with that of BLA, a general liquefying amylase.

• 자원환경지질
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• 제17권4호
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• pp.289-298
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• 1984

Uraniferous black slates of the Okchon sequence occur in Koesan (northeast) through Miwon-Boun (middle) to the southwest off Taejon (southwest) within the Okchon fold belt. The Uraniferous balck slates in the southwest off Taejon are particularly well developed in Chubu (northeast) and Moksso-ri (middle) areas whereas they are less developed in Jinsan (southwest) area. The uraniferous beds range from less than a meter to 40 meters in thickness and range from less than 0.02% $U_3O_8$ (cut-off-grade) to 0.05% $U_3O_8$ in the southwestern district off Taejon. Electron microprobe analysis of uranium-minerals found in graphitic slate samples enables to estimate their major compositions semi-quantitatively so that uraninite, ferro-uranophane and chlopinite are tentatively identified. Uranium-minerals are closely associated with carbon and metal sulfides. Correlation analysis of trace element concentrations revealed that U and F.C., and U and Mo are lineary correlative respectively and their correlation coefficients are positively high whereas those of U and V, U and Mn, and U and Zr are negatively low, implying that uranium mineralization has been closely related with concentrations of carbon and molybdenum. Stable isotope analyses of pyrite sulfur range widely from +11.5% to -23.3% in ${\delta}^{34}S$ values whereas those of graphite carbon fall within a narrow range between -23.3% and -28.9% in ${\delta}^{13}C$ values. The wide range of ${\delta}^{34}S$ values suggests that the sulfur could be of meteoric origin rather than of igneous source. The narrow range of ${\delta}^{13}C$ values, which are close to those of coal, indicates that the graphite is organic carbon in origin. Therefore, it is concluded that the uranium mineralization in the Okchon sequence took place primarily in sedimentary environment rich in organic matter and sulfide ion, both of which served as the reducing agents to convert soluble uranyl complex to insoluble uranium dioxide.

• 한국터널지하공간학회 논문집
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• 제13권3호
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• pp.215-231
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• 2011

본 논문에서는 연직 불연속면을 포함하는 지반에서 얕은 심도 2-arch 터널 굴착에 따른 거동특성을 실험적으로 연구하였으며, 2-arch 터널 굴착에 따른 필러부 하중전이 특성을 관찰하였다. 연직 불연속면의 위치를 변화시키고, 2-arch터널 시공단계별로 모형실험을 수행하였다. 실험결과, 2-arch 터널 굴착에 따른 이완하중이 불연속면이 위치한 곳에 집중되었고, 불연속면에 차단되어 불연속면을 넘어서까지 하중이 전이되지는 않았다. 또한 인접한 터널의 하반을 굴착할 때보다 상반을 굴착할 때에 필러부와 지반변형에 더 큰 영향을 미치는 것으로 나타났다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제30권3호
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• pp.186-192
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• 2004

Purpose: Human tooth proteins are highly heterogeneous, comprising diverse proteins derived from a number of genes. The attempts to identify protein for activity of tooth matrix proteins have been defied by several factors. First, the amount of proteins within teeth is very small relative to many extracellular matrix proteins of other tissues. Second, the bioassay system is tedious and needed for long time. Therefore we tried to find easy techniques, which increase the product rate, and an assay of small proteins, with which amino acid sequence is possible without additional procedures. Materials and Methods: Total protein were extracted from 300 g enamel removed teeth and 600 g teeth with 4 mol/L guanidine HCl and purified by gel chromatography. Aliquot of proteins was implanted into muscle pouches in Sprague-Dawley rats for bioassay. By SDS-PAGE and membrane blotting, molecular weight of each protein was estimated and a partial amino acid sequence was obtained. Each fraction blotted on the membrane was cut out and inserted in rat ectopic model. Results: In dissociative method, total tooth proteins were obtained 1mg/ml from enamel removed teeth and 3.5 mg/ml from teeth. In SDS-PAGE, four clear bands at the sites corresponding to 66, 40, 20 and 18 kD. Especially The 66 kD band was clearly exhibited. Amino acid sequencing from tooth could be possible using PVDF membrane blotting technique. In amino acid sequencing, 66 kD protein was identified as albumin. Conclusion: Compared with conventional method for extraction of teeth protein and bioassay of proteins, the methods in this study were easy, time-saving and more productive technique. The matured tooth proteins omitting additional procedure of mechanical removal of enamel were simply analyzed using blotted PVDF membrane. This method seems to make a contribution as a technique for bioassay and amino acid sequencing of protein.

• 한국통신학회논문지
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• 제27권8A호
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• pp.786-795
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• 2002

본 논문에서는 계층적 은닉 마코프 모델을 이용한 히스토그램과 모우멘트 기반의 동영상 장면전환 검출 방법을 제안한다. 제안된 방법은 웨이블릿 변환된 영상의 저주파 부 밴드로부터 히스토그램을 추출하며, 고주파 부 밴드로부터는 방향성 모우멘트를 추출한다. 그리고 수동적으로 분할된 비디오로부터 추출한 히스토그램 차와 모우멘트 차를 관측값으로 사용하여 은닉 마코프 모델을 학습한다. 비디오 분할 과정은 두 단계로 구성되는데, 먼저 히스토그램 기반의 은닉 마코프 모델은 입력된 비디오에 대하여 셧, 컷, 그리고 점진적인 장면전환의 3개의 범주로 분할한다. 그리고 두 번째 단계에서는 모우멘트 기반의 은닉 마코프 모델을 사용하여 점진적인 장면 전환을 더 세밀하게 페이드와 디졸브로 분할한다. 실험결과 제안된 방법은 기존의 경계값 기반의 방법보다 더 효율적으로 동영상의 셧 경계를 분할하였음을 볼 수 있었다.

• 자원환경지질
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• 제5권1호
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• pp.1-20
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• 1972

A large anorthositic mass outcropped as mushroom-like body extending up to 46km which occurs in the Hadong kaoline district of southern Korea. The anorthositic mass is in contact with the metamorphic, plutonic and sedimentary rocks. The metamorphic rocks are of granitic gneiss and banded gneiss, etc; the plutonic rocks are of gabbroic and dioritic rocks, schistose granite, syenite, diorite and granite. The sedimentary rocks include siltstone and pebbly sandstone of Lower Gyeongsang System, Cretaceous in age. The anorthositic mass shows a gradational contact with the metamorphic and sedimentary rocks, and is cut by the plutonic rocks except gabbroic and dioritic rocks. The anorthositic mass is leucocratic in the central portion of the mass, and, in turn, grades to rock phases in which ma/ic minerals are irregularly scattered, then to the well-lineated rock and finally to the banded gneiss. Lineation of the anorthositic mass is accordant with that of the surrounding banded gneiss, and the lineation continues toward the gneiss. In some places, the rock phases in which mafics are scattered is gradational with adjacent sedimentary rocks. The anorthositic mass in contact with gabbroic and dioritic rocks shows spotted features. Various replacement features seen under the microscope and paragenetic sequence of the mineral components in the anorthositic rocks cannot be considered as the origin of magmatic crystallization. From the field and microscopic observations, it is concluded that the anorthositic mass was formed from replacement of the metamorphic rocks and plutonic rocks by the anorthositic magma.

• Parasites, Hosts and Diseases
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• 제55권6호
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• pp.653-658
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• 2017

Hypoderma spp. larvae cause subcutaneous myiasis in several animal species. The objective of the present investigation was to identify and characterize morphologically and molecularly the larvae of Hypoderma spp. collected from cattle (Bos taurus taurus) and red deer (Cervus elaphus) in the district of Castelo Branco, Portugal. For this purpose, a total of 8 larvae were collected from cattle (n=2) and red deer (n=6). After morphological identification of Hypoderma spp. larvae, molecular characterization was based on PCR-RFLP and mitochondrial CO1 gene sequence analysis. All larvae were morphologically characterized as the third instar larvae (L3) of H. actaeon. Two restriction enzymes were used for molecular identification of the larvae. TaqI restriction enzyme was not able to cut H. actaeon. However, MboII restriction enzyme differentiated Hypoderma species showing 210 and 450 bp bands in H. actaeon. Furthermore, according to the alignment of the mt-CO1 gene sequences of Hypoderma species and to PCR-RFLP findings, all the identified Hypoderma larvae were confirmed as H. actaeon. This is the first report of identification of Hypoderma spp. (Diptera; Oestridae) from cattle and red deer in Portugal, based on morphological and molecular analyses.

• Journal of Mechanical Science and Technology
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• 제16권3호
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• pp.292-301
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• 2002

Ultrasound waves interact strongly with the orientation and sequence of the plies in a layup when propagating in the thickness direction of composite laminates. Also the layup orientation greatly influences its properties in a composite laminate. If the layup orientation of a ply is misaligned, it could result in the part being rejected and discarded. Now, most researchers cut a small coupon from the waste edge and use a microscope to optically verify the ply sequences on important parts. This may add a substantial cost to the production since the test is both labor intensive and performed after the part is cured. A nondestructive technique would be very beneficial, which could be used to test the part after curing and requires less time than the optical test. Therefore we have developed, reduced, and implemented a novel ply-by-ply vector decomposition model for composite laminates fabricated from unidirectional plies. This model decomposes the transmission of a linearly polarized ultrasound wave into orthogonal components through each ply of a laminate. High probability is found, by comparisons between the model and tests, in characterizing cured layups of the laminates by using the proposed method.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2001년도 춘계학술대회논문집A
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• pp.401-406
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• 2001

When propagating the thickness direction of composite laminates ultrasound waves interacts strongly with the orientation and sequence of the plies in a layup. Also the layup orientation greatly influences its properties in a composite laminate. If one ply of the layup orientation is misaligned, it could result in the part being rejected and discarded. Now, most researchers cut a small coupon from the waste edge and use a microscope to optically verify the ply sequences on important parts. Those may add a substantial cost to the product since the test is both labor hard and performed after the part is cured. A nondestructive technique would be very beneficial, which could be used to test the part after curing and require less time than the optical test. Therefore we have developed, reduced, and implemented a novel ply-by-ply vector decomposition model for composite lam mates fabricated from unidirectional plies. This model decomposes the transmission of a linearly polarized ultrasound wave into orthogonal components through each ply of a laminate. It is found that a high probability shows between the model and tests developed in characterizing cured layups of the laminates.

• 한국정보처리학회논문지
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• 제6권4호
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• pp.868-876
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• 1999

본 논문에서는 MPEG으로 압축된 비디오(MPEG-compressed video)를 대상으로 내용기반 색인(content-based indexing)에 기초가 될 동영상 자도 d분할에 관한 효과적인 방법을 제안한다. 제안하는 방법은 MPEG 시퀀스의Ⅰ(Intra), P(Predictive), B(bidirectional) 픽쳐 구성에 구애받지 않고 장면 전환점(scene change)을 검출해 낸다. 컷(cut) 검출을 위해서는 Ⅰ픽쳐의 dc 계수와 P, B 픽쳐의 매크로 블록 참조 특성을 이용하여 차이 측도(difference measure)를 설정한다. 그리고 점진적인 (gradual)장면 전환에서는 p, B 픽쳐의 참조 블록 비율을 이용하여 정확하게 장면 전환 지점을 검출한다. 이때 MPEG 시퀀스를 완전히 복원하지 않고 필요한 데이터만을 추출해 내어 전체 데이터 처리 과정을 좀 더 효율적으로 구성한다. 차이 척도의 성능과 검출 결과는 정확도(precision)와 완전추출도(recall)를 기준으로 비교분석하고, 제안한 방법을 다양한 MPEG 시퀀스에 적용시켜 검출 결과와 수행 시간 측면에서 그 효율성을 확인하였다.