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Effects of Sowing Mixed Legume Forage and Applying Cattle Manure on the Productivity, Feed Values and Organic Hanwoo Feeding Capacity of Whole Crop Barley (청보리 재배 시 콩과 사료작물의 혼파와 우분의 시용이 생산성, 사료가치 및 유기한우 사육능력에 미치는 영향)

  • Jo, Ik Hwan
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.34 no.4
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    • pp.254-261
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    • 2014
  • This study was to assess the effect of sowing mixed legume forage and applying cattle manure on the productivity and feed value of whole-crop barley (WCB) as a representative of winter crops and to estimate feeding capacity of Korean native cattle (Hanwoo) per unit area by defining optimal application level of cattle manure for more fertile soil and manure recycling for the purpose of reduced environmental pollution and efficient production of organic forage contributable to production of organic animal products. Sowing mixed legume forage significantly (p<0.05) increased yields of dry matter (DM), crude protein (CP) and total digestible nutrients (TDN) for WCB compared to WCB alone sowing, but there was no difference between mixed sowing treatments. The yields of DM, CP and TDN were significantly (p<0.05) highest in 100~150 kg N/hg. The CP and acid detergent fiber (ADF) contents were highest in mixed sowing with hairy vetch, followed by sowing mixed forage pea and WCB alone (p<0.05). There were no differences in CP and neutral detergent fiber (NDF) contents among cattle manure application levels. As nitrogen application level increased, ADF content increased (p<0.05), but TDN content decreased (p<0.05). Feeding capacity of organic Hanwoo (head/ha) weighing 450 kg of body weight with 400 g of daily gain significantly (p<0.05) increased due to mixed sowing with legume forage, and with increasing application levels of fermented cattle manure, feeding capacity significantly (p<0.05) increased. The results indicate that mixed-sowing of WCB and legume forage not only cut nitrogen application level of fermented cattle manure by over 50 kg per ha but also increased their feed value and productivity, and feeding capacity. This would be contributable to diversity of forage resources as well as production of organic animal product by creating low cost and high quality of forage.

Effect of mixed Cropping with Legume and using fermented Cattle Manure on Productivity of Whole Crop Barley (Hordeum vulgare L.) and Organic Hanwoo feeding Capacity in Gyeongbuk Region of Korea (경북지역에서 콩과 사료작물의 혼파 재배와 발효 우분의 시용이 청보리의 생산성과 유기 한우 사육능력에 미치는 영향)

  • Jo, Ik-Hwan
    • Korean Journal of Organic Agriculture
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    • v.24 no.3
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    • pp.525-537
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    • 2016
  • This study was aimed to grow organic forage as a prime requirement for organic Hanwoo cattle production over a period of 3 years (2012~2015) in Gyeongbuk region, Korea through mixed cropping of winter fodder barley with legume and application of fermented cattle manure. The effect of barley-legume mixed cropping and application of different levels of fermented cattle manure was studied on forage productivity of whole-crop barley (WCB) and evaluation of feeding capacity of Korean native cattle (Hanwoo) per unit area. The collected data was analyzed using SAS 9.3 software. Barley-legume mixed cropping increased (P<0.05) yields of dry matter (DM), crude protein (CP) and total digestible nutrients (TDN) as compared to those of sole WCB as monoculture. Similarly, acid detergent fiber (ADF) contents and relative feed value (RFV) were also higher (P<0.05) in barley-legume mixed sowing forage compared with those of sole WCB as monoculture. In case of manure application, yields of DM, CP and TDN were also found (P<0.05) highest in 100~150 kg N/ha. However, varying cattle manure levels did not influence (P>0.05) CP and neutral detergent fiber (NDF) contents. In response to increasing nitrogen application level, ADF content increased (P<0.05) but TDN content and RFV decreased (P<0.05). The Barley-legume mixed sowing and increasing application levels of fermented cattle manure also significantly enhanced (P<0.05) feeding capacity of organic Hanwoo (head/ha) having 450 kg body weight with 400 g of daily gain. Findings of present study indicated that barley-legume biculture not only cut nitrogen application level of fermented cattle manure by over 50 kg per ha but also increased their feed value and productivity of forage as compared to WCB monoculture. This would be contribution to the diversity of forage resources as well as production of organic animal product by creating low cost and high quality organic forage.

Effect of Different Growing Stages of Winter Cereal Crops on the Quality of Silage Materials and Silages (맥류의 수확시기가 사일리지의 재료적 특성 및 품질에 미치는 영향)

  • Heo, J.M.;Lee, S.K.;Lee, I.D.;Lee, B.D.;Bae, H.C.
    • Journal of Animal Science and Technology
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    • v.47 no.5
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    • pp.877-890
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    • 2005
  • This study was carried out to determine the effect of different growing stages of winter cereal crops on the quality of silage materials and silages. Silages were made from the silage materials harvested at four growing stages(boot, heading, flowering, and yellow ripe) of barley, rye, oat, and wheat. Approximately 1 kg of silage materials harvested from each growing stage stored in vinyl bags with vacuum packing method and fermented at room temperature for 40 days. As the growing stages progressed, the moisture and crude protein contents of the silage materials decreased, and fiber contents(NDF, ADF and hemicellulose) increased. All the silage materials showed significantly higher contents of water soluble carbohydrate in the boot stages than in the flowering and yellow ripe stages. There was no tendency in acetic acid contents of silage materials cut at different growing stages. The overall pH of silage materials were in the range of 5.91-6.01, and there was no significant difference among growing stages. Buffering capacity of silage materials were in the range of 26.23-29.47meq/100g DM, and showed a tendency to decline as the growing stages proceeded. The moisture and crude protein contents of silages decreased significantly in all species as the growing stages proceeded, and the fiber contents vice versa. As the growing stages proceeded, the pH of the silages tended to increase, and the acetic, butyric, and lactic acid contents tended to decrease. The buffering capacity of silages had a tendency to decrease as the growing stages of winter cereal crops proceeded. Therefore, these features described above should be taken into consideration in order to make silages from winter crops economically.

A LIGHT AND ELECTRON MICROSCOPIC STUDY OF CHANCES IN INTERMAXILLARY SUTURE DURING THE RAPID MAXILLARY EXPANSION OF YOUNG ADULT DOGS (유성견 급속정중이개시 정중구개봉합부위의 조직학적 변화에 대한 연구)

  • Lee, Doo-Hee;Chung, Kyu-Rhim
    • The korean journal of orthodontics
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    • v.26 no.2 s.55
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    • pp.153-162
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    • 1996
  • Rapid maxillary expansion is widely used for the correction of anteroposterior discrepancies, constriction of the maxillary arch, etc. This experiment was undertaken to examine the serial changes in the osteogenesis as well as the collagen fiber bundles in the intermaxillary suture during the rapid maxillary expansion treatment. Four young female dogs aged 6 to 8 months old and not showing menarche yet were used for the experiment. The maxillary impression of dogs were taken, expansion device cast and Hyrax screw soldered at the midline in the 1st premolar area. RME device was delivered to the dogs and the activation of 0.25 mm per quarter-turn was done 2 times per day for 10 days until 5 mm separation was made. Separation of the maxilla was confirmed by X-ray. The animals were sacrificed on 0, 15, 30, 60 days from the finish of maxillary separation and preparations for light microscopy and surface electron microscopy were made. The sutures were cut into frontal serial sections for examination of the histological reactions. The following results were obtained and the conclusions made. 1. The edges of the two palatal plates bordering the midpalatal suture which at the beginning of the retention period were mainly composed of compact bone, underwent extensive resorption followed by new bone formation and gradually became spongy bone rich in bone marrow which in the 60 day retention animal became the compact bone with short intermaxillary suture space. During this transformation, newly formed trabecular bone tissues were added to the original margin. 2. Throughout the expansion period, the collagen fibers underwent successive changes such as stretching, loss of polarity, and finally fibrillogenesis. Towards the end of the expansion procedure, sharpey's fiber formation in newly formed bones were observed. 3. Bony spicules were found in the initial stage of retention on occlusal topographic X-rays, which later were confirmed to have ossified. 4. Judging from the histological changes occuring during the experimental expansion, excessive expansion will cause an excessive bleeding, and retard the remodeling of intermaxillary suture. According to the above results, the bone remodeling after rapid maxillary expansion was preceded by the migration of migratory cells into the intermaxillary suture area. The bone remodeling phenomena were on-going during the 2 months retention sample.

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Studies on Cropping System for Year-Round Cultivation of Forage Crops in Gyeongnam Province (경남지방에서 조사료 주년생산 작부체계에 관한 연구)

  • Kang, Dal-Soon;Kim, Dae-Ho;Shin, Hyun-Yul;Son, Gil-Man;Rho, Chi-Woong;Kim, Jung-Gon
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.29 no.2
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    • pp.137-152
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    • 2009
  • Present experiment was conducted at the field of Gyeongnam Agricultural Research and Extension Services in Jinju city for two continuous cropping seasons to develop several adaptable and valuable year-round forage-producing system for elevating self-sufficiency and dollar-saving by reduced importing of crude forage. Twenty cropping systems were tested in experiment using whole crop barley (WCB), oat, rye, Italian ryegrass (IRG), and triticale in winter season and com, sorghum, sorghum ${\times}$ sudangrass hybrid, and oat in summer time. Sorghum ${\times}$ sudangrass hybrid showed highest fresh forage yield among experimented summer season crops, and followed com. Com produced the most dry matter yield, and followed sorghum${\times}$sudangrass hybrid, sorghum and oat in order. There was no significant effect of former winter crops on fresh and dry matter production succeeding summer time crops. Among winter season forage crops tested, oat showed the highest fresh and dry matter when clipped on mid-May, and followed triticale, IRG, rye and WCB. Winter-time cultivated crops showed no clear effect on the growth and forage (fresh and dry matter) producing ability of following summer crops. There was the most protein content in oat plant among summer season planted crops, and in sorghum for acid detergent fiber (ADF) and in sorghum ${\times}$ sudangrass hybrid for neutral detergent fiber (NDF), respectively. While, com showed highest value of relative feed value (RFV) and total digestive nutrients (TDN) among those crops. Among winter crops, the highest crude protein was in oat plant showing no significant differences of ADF and NDF, while, relatively higher value of RFV was recognized with rye and triticale. Also, triticale contained more TDN as compare to other forage crops. The cropping combinations such as com followed by (fb) rye and maize fb triticale were regarded as promising systems having higher dry matter producing ability among tested combinations. Considering TDN producing potential, the combinations with sorghum ${\times}$ sudangrass hybrid fb triticale andlor rye were would be suitable ones, coincidently. There was a tendency which elevating pH, electric conductivity (EC) and organic matter (OM) contents in soil after experiment comparing to before planting. More crude protein content in plant was shown at mid-May clipping as compared to the forage at April cut in all winter season grown crops. ADF and NDF contents were increased by delayed clipping showing decreased tendency of RFV and TDN in plant. In conclusion, many cropping systems would be available using above mentioned forage crops according to farmer's conditions and scale, etc.

A Study of the Effect of the Permeability and Selectivity on the Performance of Membrane System Design (분리막 투과도와 분리도 인자의 시스템 설계 효과 연구)

  • Shin, Mi-Soo;Jang, Dongsoon;Lee, Yongguk
    • Journal of Korean Society of Environmental Engineers
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    • v.38 no.12
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    • pp.656-661
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    • 2016
  • Manufacturing membrane materials with high selectivity and permeability is quite desirable but practically not possible, since the permeability and selectivity are usually inversely proportional. From the viewpoint of reducing the cost of $CO_2$ capture, module performance is even more important than the performance of membrane materials itself, which is affected by the permeance of the membrane (P, stagecut) and selectivity (S). As a typical example, when the mixture with a composition of 13% $CO_2$ and 87% of $N_2$ is fed into the module with 10% stage cut and selectivity 5, in the 10 parts of the permeate, $CO_2$ represents 4.28 parts and $N_2$ represents 5.72 parts. In this case, the $CO_2$ concentration in the permeate is 42.8% and the recovery rate of $CO_2$ in this first separation appears as 4.28/13 = 32.9%. When permeance and selectivity are doubled, however, from 10% to 20% and from 5 to 10, respectively, the $CO_2$ concentration in the permeant becomes 64.5% and the recovery rate is 12.9/13 = 99.2%. Since in this case, most of the $CO_2$ is separated, this may be the ideal condition. For a given feed concentration, the $CO_2$ concentration in the separated gas decreases if permeance is larger than the threshold value for complete recovery at a given selectivity. Conversely, for a given permeance, increasing the selectivity over the threshold value does not improve the process further. For a given initial feed gas concentration, if permeance or selectivity is larger than that required for the complete separation of $CO_2$, the process becomes less efficient. From all these considerations, we can see that there exists an optimum design for a given set of conditions.

The Effect of Maturity Stage and Particle Length of Sorghum-Sudan Hybrid on the Quality of Silage (Sorghum-Sudan Hybrid의 생육시기와 절단길이가 Silage의 품종에 미치는 영향)

  • 최낙민;문영식;고영두
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.5 no.1
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    • pp.73-78
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    • 1985
  • The purpose of this study is to examine the effect of maturity stages and particle length on quality of silage made from Sorghum-sudan hybrid (Pioneer 988). The silage was made at heading, milky, dough and yellow ripe stage and cut 1.0, 2.5, 4.0cm at each stage. The quality of the silage was evaluated on the bases of the chemical compositions, content of organic acids, pH and $NH_3-N$ of the silage, and also the correlation between main compositions of the silage. The results were summarized as follows; 1. The moisture content was decreased with advancing maturity, but dry matter content was increased. Crude protein was decreased with advancing maturity at the level of 12.55 percentageat heading stage. Crude fiber was tended to increase. 2. The moisture content of silage was markedly decreased with advancing maturity (p<0.01). 3. The pH value of silage was the lowest when particle length was 2.5cm, and there was no significant difference between particle lengths. At the yellow ripe stage, the pH value was the lowest (3.53) and at the dough stage, the pH value was the highest (4.59) (p<0.01). 4. The rate of $NH_3-N$ to total-N was the highest (16.3%) at heading stage, the lowest (9.2%) at the dough stage. 5. The organic acid contents was not uniformly fixed at the particle length, but the highest quality silage was produced at yellow ripe stage as was not produced butyric acid and plenty of lactic acid was contented. 6. The correlations between pH and lactic acid (r=0.719), pH and total acid (r=-0.716), butyric acid and Flieg's score (r=0.872) were negative, respectively (p<0.01). And those between lactic acid and total acid (r=0.990), moisture and $NH_3-N$ (r=0.767) were positive, respectively (p<0.01).

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A STUDY ON THE DISTRIBUTION OF CALCITONIN GENE-RELATED PEPTIDE CONTAINING NERVE FIBERS IN RAT PULP FOLLOWING DENTINAL INJURY (상아질 손상 후 흰쥐 대구치 치수의 calcitonin gene-related peptide(CGRP) 함유 신경섬유 분포에 관한 연구)

  • Moon, Joo-Hoon;Park, Sang-Jin;Min, Byung-Soon;Choi, Ho-Young;Cho, Gi-Woon
    • Restorative Dentistry and Endodontics
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    • v.24 no.1
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    • pp.100-115
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    • 1999
  • The purpose of this study was to investigate the distribution of calcitonin gene-related peptide containing nerve fibers in rat pulp after dentinl injury by means of immunohistochemistry and confocal laser scanning microscope. The Spague-Dawley rats weighing about 250-300gm were used. The animals were devided into normal control and experimental groups. Experimental animals were sacrified 1, 2, 4, 7, 10, 21days after dentinal injury (dentin cutting, and then acid etching with 35% phosphoric acid) on the maxillary molar teeth. The maxillary teeth and alveolar bone were removed and immersed in the 4% paraformaldehyde in 0.1M phosphate buffer (pH 7.4), then were decalcified with 15% formic acid for 10 days. Serial frozen $50{\mu}m$ thick sections were cut on a cryostat. The rabbit CGRP antibody was used as a primary antibody with a dilution of 1:2000 in 0.01M PB. The sections were incubated for 48 hours at $4^{\circ}C$, and placed into biotinylated antirabbit Ig G as a secondary anti body with dilution of 1:200 in 0.01M PB and incubated in ABC(avidin-biotin complex). The peroxidase reaction was visualized by incubating the sections in 0.05% 3,3 diaminobenzidine tetrahydrochloride containing 0.02% $H_2O_2$. For the confocal laser scanning microscopic examination, Primary antibody reaction was same as immunoperoxidase stainning, but fluorescein isothiocyanate(FITC)-conjugate antirabbit IgG as a secondary antibody was used. The confocal laser scanning microscope was used for the examination. A series of images of optical sections was collected with a 20x objective at $3{\mu}m$ intervals throughout the depth of specimen. FITC fluerescence was registrated through a 488nm and 568nm excitation filter, and images were saved on optical disk. The stereoscopic images and three dimentionnal images were reconstructed by computer software, and then were analyzed. The results were as follows : 1. In normal control group, CGRP containing nerve fibers were coursed through the root with very little branching, and then formed a dense network of terminals in coronal pulp. 2. A slight increase in CGRP containing nerve fibers at 1 and 2day postinjury was noted subjacent to the injury site. In the 4day group, there were an extensive increase in the number of reactive fibers, followed by a partial return toward normal levels at 7~10 day postinjury, and return by 21days. 3. The sprouting of the CGRP containing nerve fibers was evident within 2day after dentinal injury, and by 4days there was a maximal increased, but was decreased at 7days and returned to normal 10~21 day postinjury. 4. In confocal laser scanning microscopic exammination, the distinct distribution pattern and sprouting reaction of CGRP containing nerve fibers were observed in stereoscopic images and three dimentional images. These results suggest that CGRP containing nerve fiber can be important role in the response to dental injury and pain regulation.

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Progress of Composite Fabrication Technologies with the Use of Machinery

  • Choi, Byung-Keun;Kim, Yun-Hae;Ha, Jin-Cheol;Lee, Jin-Woo;Park, Jun-Mu;Park, Soo-Jeong;Moon, Kyung-Man;Chung, Won-Jee;Kim, Man-Soo
    • International Journal of Ocean System Engineering
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    • v.2 no.3
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    • pp.185-194
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    • 2012
  • A Macroscopic combination of two or more distinct materials is commonly referred to as a "Composite Material", having been designed mechanically and chemically superior in function and characteristic than its individual constituent materials. Composite materials are used not only for aerospace and military, but also heavily used in boat/ship building and general composite industries which we are seeing increasingly more. Regardless of the various applications for composite materials, the industry is still limited and requires better fabrication technology and methodology in order to expand and grow. An example of this is that the majority of fabrication facilities nearby still use an antiquated wet lay-up process where fabrication still requires manual hand labor in a 3D environment impeding productivity of composite product design advancement. As an expert in the advanced composites field, I have developed fabrication skills with the use of machinery based on my past composite experience. In autumn 2011, the Korea government confirmed to fund my project. It is the development of a composite sanding machine. I began development of this semi-robotic prototype beginning in 2009. It has possibilities of replacing or augmenting the exhaustive and difficult jobs performed by human hands, such as sanding, grinding, blasting, and polishing in most often, very awkward conditions, and is also will boost productivity, improve surface quality, cut abrasive costs, eliminate vibration injuries, and protect workers from exposure to dust and airborne contamination. Ease of control and operation of the equipment in or outside of the sanding room is a key benefit to end-users. It will prove to be much more economical than normal robotics and minimize errors that commonly occur in factories. The key components and their technologies are a 360 degree rotational shoulder and a wrist that is controlled under PLC controller and joystick manual mode. Development on both of the key modules is complete and are now operational. The Korean government fund boosted my development and I expect to complete full scale development no later than 3rd quarter 2012. Even with the advantages of composite materials, there is still the need to repair or to maintain composite products with a higher level of technology. I have learned many composite repair skills on composite airframe since many composite fabrication skills including repair, requires training for non aerospace applications. The wind energy market is now requiring much larger blades in order to generate more electrical energy for wind farms. One single blade is commonly 50 meters or longer now. When a wind blade becomes damaged from external forces, on-site repair is required on the columns even under strong wind and freezing temperature conditions. In order to correctly obtain polymerization, the repair must be performed on the damaged area within a very limited time. The use of pre-impregnated glass fabric and heating silicone pad and a hot bonder acting precise heating control are surely required.

Development of an Eating Habit Checklist for Screening Elementary School Children at High Risk of Energy Overintake (초등학생의 에너지 과잉섭취 위험 진단을 위한 식습관평가표 개발)

  • Yon, Mi-Yong;Hyun, Tai-Sun
    • Journal of Nutrition and Health
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    • v.41 no.5
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    • pp.414-427
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    • 2008
  • The purpose of the study was to develop an eating habit checklist for screening elementary school children at high risk of energy overintake. Dietary habits, food intake, anthropometric data were collected from 142 children (80 boys and 62 girls) in the 4th to 6th grades of elementary schools. Energy intake, fat intake, and percentage of Estimated Energy Requirement (%EER) were used as indices to detect the risk of energy overintake of the children. Pearson correlation coefficients were calculated between dietary habit scores and energy overintake indices in order to select questions included in the checklist. TV watching during the meal, meal speed, meal amount, overintake frequency, eatingout frequency, snack frequency, frequency of eating Ramyun or fast foods showed significant correlations with energy overintake indices. Stepwise regression analysis was performed to give each item a different weight by prediction strength. To determine the cut-off point of the test score, sensitivity, specificity, and positive predictive values were calculated. The 7-item checklist with test results from 0 to 13 points was developed, and those with equal or higher than 5 points were diagnosed as a risk group of energy overintake. Among our subjects 13.4% was diagnosed as the risk group. Mean energy intake of the subjects in the risk group and the normal group were 2,650 kcal and 1,640 kcal, respectively. However, there were no significant differences of Index of Nutritional Quality (INQ) of the other nutrients except eating fiber between the risk group and the normal group. This checklist will provide a useful screening tool to identify children at high risk of energy overintake.